• BMB Reports
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• 제32권5호
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• pp.468-473
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• 1999

Nuclear Factor 1 (NF1) proteins are a family of transcriptional factors consisting of four different types: NF1-A, -B, -C, and -X. Some NF1 transcription factors contain a heptasequence motif, SPTSPSY, which is found as a repeat sequence in the carboxy terminal domain (CTD) of the largest subunit of RNA polymerase II. A similar heptasequence, SPTSPTY, is contained in rat liver NF1-A at a position between residues 469 and 475. In order to investigate the roles of the individual amino acids of the heptasequence of rat liver NF1-A in transcriptional activation, we systematically substituted single and multiple amino acid residues with alanine residue(s) and evaluated the transcriptional activities of the mutated NF1-A. Substitution of a single amino acid reduced transcriptional activity by 10 to 30%, except for the proline residue at position 473, whose substitution with alanine did not affect transcriptional activity. However, changes of all four serine and threonine residues to alanine or of the tyrosine residue along with the serine residue at position 469 to alanine reduced the activity to almost background levels. Our results indicate that multiple serine and threonine residues, rather than a single residue, may be involved in the modulation of the transcriptional activities of the factor. Involvement of the tyrosine residue is also implicated.

• 한국응용과학기술학회지
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• 제37권2호
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• pp.318-327
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• 2020

본 연구의 목적은 신체활동에 참여하는 노인의 지각된 건강상태가 회복탄력성에 미치는 영향을 규명하는 것이었다. 이러한 목적을 달성하기 위해 총 300부의 설문지를 배포 후 회수하였고 응답내용이 부실하거나 일부 누락된 자료 18부를 제외하고 총 282부를 SPSS 19.0 Ver. 통계프로그램을 이용하여 기술통계분석, 신뢰도 분석, 탐색적 요인분석, 상관관계분석, 그리고 중다회귀분석을 실시하여 다음과 같은 결론을 얻었다. 신체활동에 참여하는 노인의 지각된 건강상태 하위 요인인 건강관심요인, 현재건강요인과 미래건강요인은 회복탄력성에 유의한 영향을 미치는 것으로 나타났다.

• 동의생리병리학회지
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• 제35권1호
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• pp.15-21
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• 2021

Donggwaja (Benincasae Semen), the seed of Benincasa hispida (Thunb.) Cogn., has been used in Korean traditional medicine to control the body heat and water retention caused by various diseases. Both the symptoms targeted by the herbal medicine in clinic and studies with disease mouse models support the potential anti-inflammatory effect of Donggwaja. However, it is less understood how Donggwaja exerts its possible anti-inflammatory effect. Here, we present evidence that Donggwaja suppresses macrophage inflammatory reactions via expressing tumor necrosis factor a-induced protein 3 (TNFAIP3 or A20) and suppressing NF-kB activity. The ethanol extract of Donggwaja (EED) showed no toxicity when added to RAW 264.7 cells less than 100mg/ml. When treating the cells for 16 h, EED significantly suppressed the nuclear localization of NF-kB, suggesting that EED suppresses NF-kB activity. Concordantly, a semi-quantitative RT-PCR analysis showed that EED decreased the expression of prototypic pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-a, IL(interleukin)-6, and IL-1b. EED induced in RAW 264.7 cells the expression of A20, a ubiquitin modulator that suppresses inflammatory signaling cascades initiated from TLR4 and TNF and IL-1 receptors, while not affecting the induction of Nrf2, an anti-inflammatory factor that could suppress the effect of NF-kB. These results suggest that EED exerts its suppressive effect on inflammation, at least in part, by expressing anti-inflammatory factor A20 and suppressing pro-inflammatory factor NF-kB activity.

• 한국실내디자인학회논문집
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• 제26호
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• pp.57-63
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• 2001

The aim of this study is to measure sensitivity reaction of human being with a physical properties of color and average size of particle for building materials and predict the influence of color and average size of particle in evaluation of building materials. As a results, 1) In results of qualitative evaluation construction, all 16 adjectives were extracted by higher evaluation items and ,total 14 adjectives were extracted as evaluation adjectives except adjectives of a contrary concept in each other. 2) According to the result of factor analysis, all 4 group of $\ulcirner$potency$\lrcirner$, $\ulcirner$activity$\lrcirner$, $\ulcirner$evaluation$\lrcirner$, $\ulcirner$warmness$\lrcirner$ were extracted. In this time, $\ulcirner$potency$\lrcirner$ as the first factor indicates the most hign original value. Consequently, $\ulcirner$potency$\lrcirner$ factor have an hign influence in evaluation of building materials. 3) As a influence analysis of evaluation variable by evaluation item $\ulcirner$potency$\lrcirner$ factor have an high influence by influence of average size of a particle, $\ulcirner$activity$\lrcirner$ factor have influence hignly by influence of brightness, $\ulcirner$evaluation$\lrcirner$ factor have an hign influence by influence of average site of a particle and the hue, $\ulcirner$warmness$\lrcirner$ factor have an hign influence mainly by influence of the hue

• 대한가정학회지
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• 제35권1호
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• pp.99-110
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• 1997

This research was focused on the identification of difficult temperament of childhood. In order to identify the difficult temperament of childhood 336 of 3 and 6 graders were investigated. the difficult temperament of childhood was defined as the cahracteristics which were perceived as difficult for taking care of by mothers related to negative mother's attitude and related to the children's low competence. Mothers felt difficult in caring for the children with high activity and negative mood. Mothers showed negative attitude to the children with negative mood and low persistence. And the children with unpredictability and low persistence had lower perceived competence. In short high activity unpredictability negative mood and low persistence might be said as difficult temperament of childhood, These temperament categories-activity predictability mood and persistence-appeared as one factor in factor analysis. unpredictability negative mood and low persistency were shown as the difficult temperament in other studies but high activity should be examined more carefully.

• 생약학회지
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• 제21권2호
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• pp.186-192
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• 1990

This study was conducted to further purify and characterize a polysaccharide fraction from whole parts of Duchesnea indica (Andr.) Focke, which was previously reported to show an antitumor activity by us. Mild acid-treatment of the fraction by 5% acetic acid yielded three subtractions (Fr. A,B and C) on a Sephadex G-25. Fr. A was composed of uronic acid, hexose, pentose and phenolic substance, Fr. C mainly phenolic substance, and Fr. B contained hexose. All three fractions exhibited colony stimulating factor activity.

• Journal of Ginseng Research
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• 제14권3호
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• pp.364-372
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• 1990

This experiment was performed to investigate the effects of ginseng saponin fractions (total saponin, triol saponin. diol saponin) and lipopolysacrharide (LPS) on the tllmoricidal activity of macrophage. The ginseng saponin fractions had little effect on the tumoricidal activity of macrophage (less than 10%). When the ginseng saponin fractions were treated with LPS, the effects of tumoricidal activation of macrophage increased a relatively high percent, and the total saponin and triol saponin (20-35%) were ulore effectual than diol saponin (15-25%). The effects of ginseng saponin and LPS on the tumoricidal activity of macrophage were mediated by the induction of macrophage-release factor(5) which has(have) the capacity of tumor cell killing. And the quantity of the (actors) was(were) increased by the contact of macrophage with tumor cell.

• Archives of Pharmacal Research
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• 제21권4호
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• pp.436-439
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• 1998

In order to find out anti-platelet activating factor (PAF) from natural resources, Korean medicinal plants used for the treatments of peripheral circulation disorders were tested for their possible protective effects on PAF-induced anaphylactic shock. From the above screening, the methanol extract of Gentiana scabra showed a potent antagonistic activity against PAF. Water suspension of the extract was partitioned with $CH_2$$CI_2$ and EtOAc, successively. The EtOAc fraction which showed the highest activity was chromatographed on silica gel to yield 6 fractions. From the fraction which showed higher PAF-antagonistic activity than the other fractions, compound 1 was isolated by recrystallization. On the basis of spectral data, compound 1 was identified as 2-hydroxy-3-methoxybenzoic acid glucose ester. The compound prevented the mice from the PAF-induced death at a dose of 300 $\mu\textrm{g}$/mouse.

• 한국기후변화학회지
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• 제9권4호
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• pp.407-421
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• 2018

In this paper, I analyze the GHG (greenhouse gas) emission factor of the domestic railway transportation sector using the LMDI (Log Mean Divisia Index) methodology. These GHG factors are the emission factor effect, energy intensity effect, transportation intensity effect, and economic activity effect. The analysis period was from 2011 to 2016, and the analysis objects were an intercity railway, wide area railway, and urban railway. The results show that the GHG emission of railway transportation sector decreased during these 6 years. The factors decreasing the GHG emission are the emission factor effect, energy intensity effect, and transportation intensity effect, while the factor increasing the GHG emission is the economic activity effect.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2010년도 춘계 학술대회 제22권1호
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• pp.307-308
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• 2010

본 연구는 플라이애시 생산지 및 로트별 품질변화에 따른 콘크리트 압축강도 발현 특성에 미치는 요인을 분석하기 위한 기초연구로, 플라이애시 생산지 및 로트별 모르타르 활성도 지수와의 상관관계를 검토하였다.