PAF Antagonistic Activity of 2-hydroxy-3-methoxybenzoic Acid Glucose Ester from Gentiana scabra

  • Huh, Hoon (College of Pharmacy, Seoul National University) ;
  • Kim, Hye-Kyong (College of Pharmacy, Seoul National University) ;
  • Lee, Hern-Ku (Medical School, Chonbuk National University)
  • Published : 1998.08.01

Abstract

In order to find out anti-platelet activating factor (PAF) from natural resources, Korean medicinal plants used for the treatments of peripheral circulation disorders were tested for their possible protective effects on PAF-induced anaphylactic shock. From the above screening, the methanol extract of Gentiana scabra showed a potent antagonistic activity against PAF. Water suspension of the extract was partitioned with $CH_2$$CI_2$ and EtOAc, successively. The EtOAc fraction which showed the highest activity was chromatographed on silica gel to yield 6 fractions. From the fraction which showed higher PAF-antagonistic activity than the other fractions, compound 1 was isolated by recrystallization. On the basis of spectral data, compound 1 was identified as 2-hydroxy-3-methoxybenzoic acid glucose ester. The compound prevented the mice from the PAF-induced death at a dose of 300 $\mu\textrm{g}$/mouse.

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