• Journal of Korean Society of Forest Science
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• v.96 no.4
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• pp.387-392
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• 2007

Characteristics of needle morphology and anatomy were examined in 14 populations of Abies nephrolepis (Trautv.) Maxim. and A. koreana Wilson. Additionally we studied the classification index to distinguish between the species by the method of discriminant analysis. Characteristics of needle for A. nephrolepis could be distinguished from those for A. koreana by flatten arrangement, thin and long length for needle form, many stomata row, and marginal position of resin duct Nested ANOVA showed that there were statistically significant differences among populations as well as among individuals within populations in all 9 needle traits. For the needle indices such as needle thickness, number of stomata row, and the distance between resin duct and vascular for both species, variance components among populations were larger than those among individuals within populations. The characteristics that contributed most to the separation of A. nephrolepis and A. koreana according to the discriminant analysis using stepdisc procedures were needle index and thickness of needle, needle arrangement index, distance between resin duct and vascular, and number of stomata row.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.358-369
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• 2011

The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from genus Chrysosplenium were sequenced to address phylogenetic relationship. ITS including 5.8S sequence varied in length from 647 bp to 653 bp. Among them, 219 sites were variable sites with parsimony-informative. The aligned sequences were analyzed by maximum parsimony (MP) and neighbor-joining (NJ) methods. In the strict consensus trees of parsimony analysis, the monophyly of Chrysosplenium was supported by 100% bootstrap value. The first clade, C. pseudofauriei was at the basal position of the genus, and others formed two clades with high bootstrap support. The second clade included Ser. Pilosa and Ser. Oppositifolia and third clade included Ser. Alternifolia and Ser. Flagellifera. The NJ trees showed essentially the same topology. Finally, DNA sequences of ITS regions were useful phylogenetic marker in this genus. Based on the ITS and ridge seed morphological results, C. sphaerospermum Maxim. and C. valdepilosum (Ohwi) S.H. Kang & J.W. Han were discussed their scientific names and taxonomic positions.

• Journal of Life Science
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• v.21 no.12
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• pp.1659-1665
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• 2011

In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.429-433
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• 2007

Shoot weight of Orostachys japonicus should determine its profitability. The study was carried out to measure the effect of nitrogen (N) fertilization level (0, 5, 10, 20kg/10a) on its growth, morphological characters, and flowering. Night-break treatment of 2 hours at midnight were done since Aug. 25 when afterward samples were taken every 4 weeks to Nov. 16. Growth, morphological and flowering related characters were measured from each sample. Plant height, inflorescence length, number of leaves including bracts and stem diameter were decreased with increased N levels. Leaf and bract, floret, shoot and total dry weights as well as withering rate showed similar response to the above morphological characters. Florets were less formed with increased N levels but were not flowered due to the night-break treatment, meaning that N fertilization had little effect on its growth and anthesis of the florets.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.40-47
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• 2009

Heavy reliance on organosynthetic chemical methods to control agricultural pest tends to deteriorate and lower fertility of soil. We studied annual occurrence of earthworm in environment-friendly agroecosystem and attempted to determine the degree of soil contamination. Candidate bioindicators of earthworm in agroecosystem were selected through analysis of earthworm diversities. Depending on the degree of pesticide use, the fields were devided into conventional, less-pesticide, pesticide free, and organic fields. A nearby field not in agricultural use was chosen as a control. This study was carried out in the greenhouses (lettuce and grape) in 2006 and in orchards (apple, pear, peach, citrus and grape) in 2007. Earthworm was collected from 50 $\times$ 50 $\times$ 10 cm$^3$ of respective soil. We found 11 species from 5 genera and 3 families, and 12 species from 4 genera and 3 families in the greenhouses and in orchards, respectively. The contents of organic matter in soil in the greenhouses were variable but quite less so in orchards. Amynthas heteropodus occurred most frequently in both sites suggesting that it could be adopted as a bioindicator in agroecosystem in Korea.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.539-547
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• 2002

본 연구는 풋콩 탈협기 개발을 위한 기초연구로서 풋콩 탈협기의 급치를 교환할 수 있고, 급동의 주속도와 송풍팬의 회전속도 및 공기 흡입구의 개구면적을 조절할 수 있는 실험장치를 제작하고, 급치의 종류가 풋콩의 탈협 및 손상에 미치는 영향을 분석하였다. 또한 송풍팬의 회전속도와 흡입 공기구의 개구비 변화 따른 선별 성능을 분석하였다. 그 결과를 요약하면 다음과 같다. 1 급치의 종류에 따른 풋콩의 미 탈협율은 철선 급치가 최대 1.6%, 최소 0.0%,평균 0.7%로서 가장 낮게 나타났고, 그 다음이 경도 60의 고무제 급치로서 최대 2.0%, 최소 0.0% 평균 1.1%, 경도 80의 고무제 급치는 최대 2.5%, 최소 0.0% 평균 1.7% 순으로 높아졌다. 또한 풋콩의 미탈협율은 급동 원주속도가 18m/s에서 55m/s로 증가할수록 감소하였다. 이는 급동의 원주 속도가 증가함에 따라 급치의 선단이 풋콩 꼬투리에 가하는 타격력이 증가하기 때문인 것으로 생각된다. 2. 급치의 종류에 따른 풋콩의 손상율은 경도 80의 고무제 급치는 최대 5.2%, 최소 1.3%, 평균 2.8%로서 가장 낮게 나타났고, 그 다음이 경도 60의 고무제 급치로서 최대 5.7%, 최소 2.4% 평균 3.8%, 철선 급치는 최대 6.3%, 최소 2.6% 평균 4.2% 순으로 높아졌다. 또한 풋콩의 손상율은 급동 원주속도가 증가할수록 풋콩 꼬투리의 손상율은 증가하였다. 이 또한 급등의 원주 속도가 증가함에 따라 급치의 선단이 풋콩 꼬투리에 가하는 타격력이 증가하기 때문인 것으로 생각된다. 3. 공기흡입구의 개구면적을 증가시킬수록 선별율은 높게 나타났으나 개구비가 60%이상에서는 선별율이 크게 차이가 나지 않았으며, 송풍팬의 원주속도를 높일수록 선별율은 높게 나타났다.도 콤바인이 직선구간 및 선회구간을 주행하며 수확작업이 가능함을 알 수 있었다. 그러나 곡선구간에서는 최대오차가 65.5 cm로 매우 크게 나타나, 콤바인을 무인 자율주행으로 수확하기에는 어려움이 있는 것으로 나타났다. 실제 포장은 이론적인 완전한 직선보다는 작은 굴곡이 있는 곡선의 형태가 이루어져 있으므로 주행 오차를 감소하기 위해서는 기계시각을 이용하면 보다 정밀한 조향을 이룰 수 있을 것으로 예상된다. 포장에서 DGPS 신호, 자이로 센서 등을 이용한 콤바인의 무인주행 장치는 무인 수확작업을 위한 가능성을 보여주었고, 일부의 센서의 기능을 개선하면 만족한 성능을 나타낼 수 있을 것으로 판단된다.dislocations form local defect arrangements at the grooves permitting the substantial reduction in defect density over the remainder of the interfacial area.한 최대의 감자 재배지역을 형성하였다. 제주도는 산지지형과 따뜻한 기온으로 2기작이 가능하고, 감자가공 공장설립과 교통발달에 따른 육지 시장과의 접근이 용이해졌기 때문에 남한에서 2번째로 큰 감자재배지역이 되었다.(요약 및 결론에서 발췌)그람양성균에서 효과적이었으며, 농도별 항균력시험 결과 농도가 증가할수록 비례하여 저해율도 증가함을 알 수 있었다. 첨가농도를 달리하여 미생물의 생육도를 측정한 결과, fraction II磎꼭\ulcorner경우 그람양성균에 대해 500 ppm 이상에서 뚜렷한 증식억제효과를 나타내었다.서 뚜렷한 증식억제효과를 나타내었다.min/+}$계 수컷 이형접합체 형질전환 마우스에 AIN-76A 정제사료만을 투여한 대조군은 1.40$\pm$

• Reproductive and Developmental Biology
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• v.30 no.3
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• pp.157-162
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• 2006

Endogenous 84 amino acid parathyroid hormone(PTH) is synthesized as a pre-pro hormone by the chief cells of the parathyroid glands. Physiological actions of PTH include regulation of bone metabolism, renal tubular reabsorption of calcium and phosphate, and intestinal calcium absorption. In addition, PTH stimulates new bone formation by extraordinary stimulation of osteoblastic activity and decreasing calcium excretion by the kidney. In this study, we constructed and tested retrovirus vectors designed to express the human parathyroid hormone(hPTH) gene under the control of the tetracycline-inducible promoters. To increase the hPTH gene expression at turn-on state, woodchuck hepatitis virus posttranscriptional regulatory element(WPRE) sequence was also introduced into retrovirus vector at downstream region of either the hPTH gene or the sequence encoding reverse tetracycline-controlled transactivator(rtTA). Transformed primary culture cells(porcine fetal fibroblast, PFF, chicken embryonic fibroblast, CEF) were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the hPTH gene expression level using two step RT-PCR and ELISA Higher hPTH expression($3{\tims}10^4\;pg/ml,\;5.3{\times}10^4\;pg/ml$) and tighter expression control(up to 8 fold) were observed from the vector in which the WPRE sequence was placed at downstream of the hPTH gene. The resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.191-197
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• 2013

Two medium-leaf ecotypes (CY6069, CY6097) belonging to one species (Zoysia japonica) of Korean lawngrasses were selected in sod production fields in Jang Seong, Korea. They were reported to have distinct morphological and growth rate characteristics different from the preferred medium-leaf type zoysiagrass in Korea. This study was conducted to define further the genotypic difference at the molecular level and to develop DNA marker based on the specific DNA fragment. Polymorphic DNA fragments were first explored by using randomly amplified polymorphic DNA (RAPD) primers, which were then converted into PCR-based sequence characterized amplified region (SCAR) markers. The CY6069-specific primer set amplified about 550 bp successfully, while the CY6097 marker produced the expected 690 bp band, by which those markers were nominated by CY6069_550 and CY6069_690 SCARs, respectively. Together with the reported morphological and other phenotypic features, the SCAR markers confirmed in this study will be useful to identify those medium-leaf zoysiagrass genotypes when they are cultivated with other vegetatively propagated warm-season turfgrasses in sod farms.

• Korean Journal of Plant Tissue Culture
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• v.25 no.3
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• pp.165-169
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• 1998

Anthers of three cross combinations of hot-pepper(Capsicum annuum L.) were cultured on Dumas De Vaulx medium supplemented with some growth regulators. The embryo production efficiency and the diversity for agronomic traits in $A_2$ lines were investigated. The embryo production frequencies of hybrid combinations were ranged from 16.4% to 43.4%, the highest embryo induction combination was DGSH $\times$ C-NH with 43.4% embryogenic efficiency. Among total 275 $A_2$ lines, phenotypic variants were found in six lines, 2.1% variant frequency. The diversity of $A_2$ lines derived from anther culture was different according to the cross combinations. Fruit color was within parental range, no transgressive variation was observed. However leaf color showed transgressive variation. In fruit length, fruit width and fruit weight, one C-HC $\times$ DGSH and DGSH $\times$ C-NH showed great diversity compared with doner parents while Cheokjo 1 $\times$ C-NH crossed with Cheokjo 1 with big fruit shape showed small diversity. Stem length to 1st branch was relatively similer to or longer than donor parents. Stem thick exhibited remarkable diversity. Node number to 1st branch distributed alomost within the range of donor parents in C-HC $\times$ DGSH combination, however great transgressive variations were observed in DGSH $\times$ C-NH and Cheokjo 1 $\times$ C-NH combinations.

• Animal Systematics, Evolution and Diversity
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• no.nspc5
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• pp.71-79
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• 2005

Two hypotrichous ciliates inhabiting the representative terrestrial habitats of Jindo Island, Holostichides chardezi Foissner, 1987 and Rigidocortex octonucleatus (Foissner, 1988) are found from Jindo Island. These two species are newly recorded from Korea. The description was based on the observation of living specimens, protargol impregnated specimens and biometric analysis. The morphological variations among the populations of these species were investigated with morphometry. The diagnostic characteristics of these species are as follows: Holostichides chardezi : elongate body flexible and twisted, yellowish subpellicular granules scattered entire cortex, adoral zone of membranelles covering about $28\%$ of body length, left and right rows of mid-ventral cirri paired, left row ended at buccal vertex and right row extending to center of body, no transverse cirri, both marginal rows of cirri not confluent at posterior end, 28-42 macronuclei and 1-5 micronuclei scattered entire body. 3 caudal cirri and 4 dorsal kineties. Rigidocortex octonucleatues: elongate-oval body rigid and not contractile, yellowish cytoplasmic crystals and fat globules densely packed, adoral zone of membranelles covering about $40\%$ of body length, 5 transverse cirri, both marginal rows of cirri not confluent at posterior end, 8 macronuclei longitudinally arranged in median of cell, 3 caudal cirri and 7-10 fragmented dorsal kineties.