Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)

'진귤' (Citrus sunki) 의 웅성가임 연관 SCAR 마커 개발

  • Chae, Chi-Won (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Dutt, Manjul (Citrus Research and Education Center, University of Florida/IFAS) ;
  • Yun, Su-Hyun (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Jae-Ho (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Dong-Hoon (Citrus Research Station, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 채치원 (국립원예특작과학원 감귤시험장) ;
  • ;
  • 윤수현 (국립원예특작과학원 감귤시험장) ;
  • 박재호 (국립원예특작과학원 감귤시험장) ;
  • 이동훈 (국립원예특작과학원 감귤시험장)
  • Received : 2011.09.02
  • Accepted : 2011.12.13
  • Published : 2011.12.31
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Abstract

In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

감귤류 중 수술이 퇴화되어 웅성불임형질을 나타내는 '청견' 품종에 정상적인 수술의 형태를 가진 웅성가임인 '진귤' 품종을 교배하여 150개체의 $F_1$ 집단을 구축하여 수술이 퇴화되는 개체와 정상인 개체를 분리하였다. 분리된 F1 개체들을 사용하여 SRAP 기법과 집단 분리 분석법(BSA)을 조합하여 웅성 가임 연관 마커 개발에 활용하였다. $F_1$ 집단 내 150개체 중 66개체가 퇴화 수술을 갖고 있으며 웅성 가임성과 웅성 불임성의 분리비는 1:1이며 $x^2$ 값은 2.16(p=0.05)이었다. 197개의 SRAP 프라이머 조합들 중 웅성가임 특이밴드를 형성하는 3개의 SRAP 프라이머 조합(F4/R27, F39/R60, 및 F15/R37)을 선발하였으며, 이 중 F39/R60 프라이머에 특이적으로 증폭하는 DNA단편의 염기서열을 기본으로 하여 새롭게 작성한 양방향 프라이머 조합 중 웅성 가임 계통에서만 약 1.4 Kb의 특이밴드를 증폭하는 프라이머 조합, pMS 33U/pMS 1462L를 선발하여 SCAR 마커를 개발 하였다. 이러한 결과는 개발된 SCAR 마커로 무핵성 계통들의 육종 선발에 효율성을 높일 수 있을 것으로 기대된다.

Keywords

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