• Title/Summary/Keyword: 침지법

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Experimental Infection for the Neutralization of White Spot Syndrome Virus (WSSV) in Wild Captured Sand Shrimp, Crangon affinis (자연산 자주새우(Crangon affinis)에서 흰반점바이러스(WSSV)의 neutralization을 위한 인위감염(experimental infection))

  • Gong, Soo-Jung;Kim, Yeong-Jin;Choi, Mi-Ran;Kim, Sung-Koo
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1294-1298
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    • 2010
  • White spot syndrome virus (WSSV) is one of the most virulent viral agents threatening the penaeid shrimp culture industry. This study was carried out to evaluate the susceptibility of the sand shrimp, Crangon affinis, to WSSV as an alternative experimental model. WSSV caused 100% mortality in C. affinis within 7 days after experimental infection by immersion. Based on challenge studies, it was confirmed that C. affinis could be a potential host in WSSV transmission. Also, the neutralization of WSSV was carried out using an antiserum raised against recombinant envelop protein rVP466 to evaluate the WSSV infection mechanism. A constant amount of WSSV (at $1{\times}10^4$ diluted stocks) was incubated with various amounts of antiserum and then mixed to 20 l reservoir for the immersion challenge of C. affinis for neutralization. At 5 days post challenge, the shrimp in the positive control immersed in the immersion reservoir containing WSSV stock showed 100% mortality. The shrimps challenged with the 3 different mixtures of WSSV and rVP466 antiserum (1:0.1, 1:0.5 and 1:1) showed 100%, 68.8% and 68.8% mortality at 14 days post challenge, respectively. These results indicated that the antiserum raised against rVP466 could block WSSV infection in C. affinis. Therefore, this study confirmed that C. affinis can be naturally infected by WSSV as another potential host and that C. affinis can be used as an alternative experimental animal instead of penaeid shrimps.

A Study of Cosmetic Sustainability Evaluation of Powder Base Make-up Products (파우더 베이스 메이크업 제품의 지속성 평가 방법 연구)

  • Lee, Sang Gil;Kim, Ki Jung;Kim, Young Ho;Pyo, Hyeong Bae;Lee, Dong Kyu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.209-218
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    • 2015
  • Once powder base makeup products are applied to the skin, the products are formed a film and attached on the skin for 8 to 12 hours. The makeup film is deformed by secretions such as sweat and sebum secreted from the skin. Thus, durability of the film is an important quality factor in the makeup and its evaluation is also important. In this study, characteristics of the semi-finished powder products such as water absorption, oil absorption, water repellent and oil repellent were evaluated in a number of ways. Also, simple methods, which are not affected by evaluation conditions such as a difference between sweat and sebum secretion, temperature and humidity, were examined to predict the durability of the products. We measured water absorption, oil absorption, water repellent and oil-repellent properties of semifinished product by tablet, capillary and dipping method and then compared with the data of color difference meter and ROBO skin analyzer. Results showed that the durability of powder base makeup products was associated with more oil absorption and water-repellent characteristics than water absorption and oil-repellent. Oil absorption characteristics by tablet and capillary method and water-repellent characteristics by dipping method provides a simple and quick method to precdict the durability of the makeup products.

Comparison of pretreatment of fallen leaves for application evaluation by Bio-ethanol raw material (바이오에탄올 원료로서 활용평가를 위한 낙엽의 전처리 비교)

  • Choi, Hyoyeon;Kim, Jaehyung;Pak, Daewon
    • Journal of Energy Engineering
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    • v.23 no.3
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    • pp.241-246
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    • 2014
  • This study is to compare characteristics of saccharification reactions applying to enzymatic hydrolysis of pretreated fallen leaves for bio-ethanol production. It experimented pretreatment of acid, alkaline in the chemical. This experiment includes preteatment of acid and alkaline in chemical, soaking, shaking and autoclaving method, which were applied to biomass. In result, the glucose production from alkaline-NaOH method was 263 mg glucose/ g biomass comparing with them of acid-HCl method. Thus, alkaline-NaOH method is superior than the acid-HCl method for chemical preteatment of fallen leaves. Also, when various chemical treatments were compared, they were all. Based on the results of this study, we found that leaves, one of biomass, are possible in pretreatment and enzymatic hydrolysis process, and they are likely to affect bio-ethanol production in the future.

Studies on the Control of Fusarium Cucurbitaceous Plants (2) On the Fungicidal Effects for the Control of Cucumber Fusarium Wilt (오이류 덩굴쪼김병 방제에 관한 연구 (2) 오이 덩굴쪼김병 방제를 위한 약제의 효과에 관하여)

  • Lee Du Hyung
    • Korean journal of applied entomology
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    • v.7
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    • pp.77-81
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    • 1969
  • The effects of submerged method. Zentmyer's drench method. pot test and field test with materials such as Carbamisol (ammonium methyldithiocarbamic acid $30\%$), NCS (ammonium N-methyldithiocarbamate$50\%$, Similuton(ethyl phenethynylmeroury $3.3\%$), Soill(N-ethylmercuric p-toluensulfonanilide $4\%$, Captan (N-trichloromethylthio-1, 2, 3, 6-tetra-hydrophthalimide $80\%$), Difolatan(N-tetrachloroet-hylthio 4cyelohexeno-1,2-dicarboximide $80\%$, Grand(2, 3-dibromepropionitryl $20\%$, trichloronitroethylene $20\%$) and Calcium hydroxide upon occurrence of Fusarium wilt of cucumber were studied. The results are summarized as follows. 1. According to submerged method, all the compounds tested were highly inhibitory to mycelial growth of Fusarium oxysporum f. cucumerinum except calcium hydroxide. But several compounds were no inhibitory to the causal fungus except Grand and Similuton, with Zentmyer's drench method. 2. Grand was the most effective fungicide for the control of cocumber Fusarium wilt in the pot test, and followed by Similuton and calcium hydroxide. The population of Fusarium oxysporm was markedly reduced when soil was treat with Grand compared with other chemicals. 3. Fungicidal effects upon occurrence of Fusarium wilt of cucumber under the field experiment varied with the fungicides. Of the fungicides tested. Grand gave the best control, and followed by Difolatan.

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A comparative study of physical properties of $TiO_2$ thin films according to a coating method on orthodontic wires and brackets (교정용 와이어 및 브라켓에 이산화티탄 광촉매 코팅 시 코팅방법에 따른 비교연구)

  • Koh, Eun-Hee;Cho, Jin-Hyoung
    • The korean journal of orthodontics
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    • v.36 no.6
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    • pp.451-464
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    • 2006
  • The purpose of this study was to search for an appropriate method of coating $TiO_2$ on orthodontic appliances. $TiO_2$ thin films were deposited on orthodontic wires and brackets using sol-gel, CVD (Chemical Vapor Deposition) and PE-CVD (Plasma Enhanced-CVD) methods. The roughness of $TiO_2$-coated surfaces was investigated via scanning electron microscope (SEM) and adhesive strength of $TiO_2$ thin films was measured by adhesive tape pull test. Methylene blue degradation test was carried out to evaluate the photocatalytic activity of $TiO_2$ and the corrosion resistance of $TiO_2$ thin films against fluoride solution was also analyzed by observing the surfaces of $TiO_2$-coated wires and brackets via SEM after immersion in sodium fluoride solution. Through the comparison of properties and photocatalytic activity of $TiO_2$ thin films according to the coating methods, the following results were obtained. Smoother surfaces of $TiO_2$ thin films were generated by CVD or PE-CVD methods than through the sol-gel method or the control. Adhesive strength of the $TiO_2$ thin films was highest in PE-CVD and gradually became lower in the order of CVD, then the sol-gel method. Photocatalytic activity of $TiO_2$ thin films on methylene blue was the highest in PE-CVD and gradually became lower in the order of CVD, then the sol-gel method. Corrosion resistance of $TiO_2$ thin films against fluoride solution was stronger in CVD and PE-CVD methods than in the sol-gel method. The results of this study suggest that the CVD or PE-CVD methods is more appropriate than the sol-gel method for $TiO_2$ coating on orthodontic wires and brackets.

The Characteristic of Different Cultivar of Okra (Abelmoschus esculentus) under the Plastic House Condition in Jeju Island (제주지역에서 오크라 무가온 재배에 따른 품종별 특성)

  • Kim, Chun-Hwan;Ahn, Yul-Kyun;Seong, Ki-Cheol;Lee, Jin-Su;Song, Uen-Young;Kim, Seong-Cheong;Moon, Doo-Kyong
    • Journal of Bio-Environment Control
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    • v.20 no.1
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    • pp.40-44
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    • 2011
  • This study was conducted to develop method of producing okra in the plastic house and the 11 cultivar of okra were planted in plastic house for selecting proper cultivar in Korea. As result of observing growth, commercial cultivar germinated better than wild collected. The germination rate in 'No 1', 'No 4' and 'No 5' were better than in 'No 2' and 'No 3'. The plant height of 'No. 4', which is one of wild collected, showed biggest plant height moreover 'Greensode'. The numbers of node, which could indicate to set fruit, showed 4.2~6.5 per plant in most cultivar and 'No 2' and 'No 5' could yield more than others. 'Greensode' was planted in plastic house for finding out suitable planting method. The seeds were soaked could germinate 100% and those were taken 8 days for germinating but control seeds needed 15 days to germinate. The yield of 'Greensode' which was planted in $45{\times}75cm$ with planted 3 and 4 per hill could best produce in plastic house.

Pretreatment sterilization of garlic and ginger using antimicrobial agents and blanching (데치기 및 초고압 처리를 이용한 항균처리 마늘 및 생강의 살균 효과 연구)

  • Choi, Jun-Bong;Cheon, Hee Soon;Chung, Myong-Soo;Cho, Won-Il
    • Korean Journal of Food Science and Technology
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    • v.50 no.2
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    • pp.172-178
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    • 2018
  • The sterilization rates were investigated to develop effective pretreatment methods of garlic and ginger using antimicrobial agents and blanching. Antibacterial components in chopped garlic and ginger, such as allicin, ginger, and shogaol, exhibited an antibacterial effect that reduced bacteria by a factor of $10^1(CFU/g)$. The total number of bacteria in garlic and ginger was decreased by $10^1(CFU/g)$ after individually soaking in 1% (w/w) citric acid, calcium oxide and 200 ppm sodium hypochlorite solution for 1 h, respectively. When chopped garlic and ginger were immersed in 1% (w/w) calcium oxide solution for 1 h, the bactericidal effect was increased by $10^4-fold$. Total cells with thermoduric bacteria exhibited a $10^3-10^4-fold$ reduction after heating for 30-40 s at $90-95^{\circ}C$. The combination of soaking in 200 ppm sodium hypochlorite solution and blanching at $95^{\circ}C$, 40 s showed $10^1-fold$ decrease in total number of cells compared to blanching in whole ginger.

Effects of Treatment Methods of Fire-retardant and Layup of Treated Veneers on the Performances of Plywoods (내화약제(耐火藥劑)의 처리방법(處理方法) 및 처리단판(處理單板)의 조판형태(調板形態)가 합판(슴板)의 성능(性能)에 미치는 영향(影響))

  • Son, Jung-Il;Cho, Jae-Sung;Suh, Jin-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.27 no.3
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    • pp.39-50
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    • 1999
  • This research was carried out to investigate the development of fire-retardancy treatment technology and performance evaluation of fire-retardant treated plywoods. Radiata pine, keruing, dillenia, calophyllum and terminalia veneers were treated by normal(conventional) pressure soak(NPS) and vacuum-pressure-soak(VPS) using 20% water solution of diammonium phosphate. Then, 4.8mm thick, 3ply plywoods were fabricated with combination of fire-retardant treated, untreated or water-immersion types and several composition types of radiata pine and keruing veneers, i,e. the uniform and the mixed types in species composition, and the homogenious and the alternate layer types in veneer treatment. In composed species, the retention and the treatment effects of fire-retardant chemicals III radiata pine was still greater than those of keruing. The effect of VPS treatment was larger than that of NPS treatment, however, adhesive bonding strength and bending strength of plywoods treated by these two methods were not necessarily lowered, compared to those of untreated plywood. And also, fire endurance performance of the urea melamine resin-bonded plywood was greater than that of the phenol resin-bonded plywood. In result, the appropriate combination in veneer species and layer as well as alternate fire-retardant treatments would be more efficiently available in service.

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Influence of Membrane Material and Structure on Fouling of a Submerged Membrane Bioreactor (침지형 막 분리 활성슬러지법에서 막의 재질 및 구조가 파울링에 미치는 영향)

  • Choi, Jae-Hoon;Kim, Hyung-Soo
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.1
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    • pp.31-36
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    • 2008
  • This work was performed to evaluate the effect of membrane material and structure on fouling in a submerged membrane bioreactor(MBR). Three types of microfiltration membranes with the same pore size of 0.1 $\mu$m but different materials, polytetrafluoroethylene (PTFE), polycarbonate(PCTE) and polyester(PETE), were used. While PETE membrane exhibited the most rapid flux decline throughout the operation, PCTE and PTFE had a similar tendency with regard to permeability. Difference in permeability between PETE and the other membranes gradually decreased with time, which was probably due to chemical cleaning. The higher TOC rejection of PETE membrane could be attributable to its faster fouling, resulting from a larger amount of foulants to get attached to the membrane in a shorter time. DOC fractionation using a DAX-8 resin showed that the composition of each fraction between the supernatant and permeates did not change significantly with operation time, indicating that membrane hydrophilicity/hydrophobicity was not a dominant factor affecting to MBR fouling in this study. Compared to other membranes, the fouling of PETE membrane was more influenced by pore clogging (irreversible fouling), which would probably contribute to a higher organic rejection of the PETE membrane.

Selection of Low Toxic Pesticides and Residual Toxicity to Cotesia glomerata (배추나비고치벌 (Cotesia glomerata)에 대한 저독성 약제 및 잔류독성)

  • Choi, Byeong-Ryeol;Lee, Si-Woo;Park, Hyung-Man
    • Korean journal of applied entomology
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    • v.46 no.2
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    • pp.251-259
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    • 2007
  • For the development of integrated pest management system by harmonizing biological and chemical control, some experiments wee carried out to select low toxic pesticides against natural enemies. and their residual toxicity were evaluated. Leaf dipping method, body dipping method, and diet treatment method were set up for the toxicity evaluation against Cotesia glomerata adults. We had tested 46 different pesticides (31 insecticides, 11 fungicides, 4 herbicides) at recommending concentration commonly used to control diamond back moth, disease and up-land weeds in chinese cabbage field. Twenty three insecticides, eleven fungicides, and four herbicides were shown to be low toxic to C. glomerata adults in the treatment of body dipping. After insecticide spraying at recommending dose on the chinese cabbage, we examined residual effect of insecticides by introducing natural enemies on different days. Safety interval for the introduction of C. glomerata adults was established according to the residual toxicity of pesticides. Safe insecticides for the introduction of C. glomerata adults at one day after treatment (DAT) were thiacloprid, acephate, chlorfenapyr, clothianidin and at 3 DAT were imidacloprid, deltamethrin, thiamethoxam, dimethylvinphos, emamectin benzoate.