• Title/Summary/Keyword: 추출제

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Effect of Medicinal Plant Water Extracts on Glucose-regulating Enzyme Activities in Goto-Kakizaki Rat Liver Cytosol (약용식물 물 추출물이 Goto-Kakizaki 흰쥐의 간 세포액에서 당대사 관련효소 활성에 미치는 영향)

  • Kim, Dae-Jung;Chung, Mi-Ja;You, Jin-Kyoun;Seo, Dong-Joo;Kim, Jeong-Mi;Choe, Myeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.10
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    • pp.1331-1335
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    • 2009
  • We have studied the anti-diabetic effects of medicinal plant water extracts on hepatic glucose-regulating enzymes such as glucokinase (GK) and acetyl-CoA carboxylase (ACC). $\alpha$-Glucosidase inhibitor is usually used to prevent and treat type II diabetes; thus, anti-$\alpha$-glucosidase activity of medicinal plant water extracts was assayed. The hepatic cytosol faction of a type II diabetic animal (Goto-Kakizaki rat) was used in GK and ACC activity assays. The medicinal plants were Lycium chinense (JGP), Discorea japonica Thunb. (SY), Pyrus pyrifolia (YSB), Cornus officinalis (SSY), Paeonia suffruticosa ANDR. (MDP), Cordyceps militaris (DCH), and Acanthopanax senticosus (GSO). JGP, SY, YSB, and SSY water extracts increased the hepatic GK activity and all medicinal plant water extracts led to an increase in hepatic ACC activity. YSB, SSY, MDP, and GSO water extracts showed significantly higher anti-$\alpha$-glucosidase activity than control samples. The highest anti-$\alpha$-glucosidase activity was observed in GSO water extract and the anti-$\alpha$-glucoside activity was higher than that of Acarbose (reference $\alpha$-glucosidase inhibitor). We suggest that JGP, SY, YSB, and SSY water extracts may exert an anti-diabetic effect by enhancing the glucose metabolism and that YSB, MDP and GSO may be used as natural $\alpha$-glucosidase inhibitors in type II diabetic conditions. Increased ACC activity by plant water extracts may provide additional anti-diabetic effect.

Separation of Caffeine and Catechin Compounds from Green Tea by Quercetin Molecular Imprinted Solid-Phase Extraction (케르세틴 분자각인 고정상 추출을 이용한 녹차에서 카페인 및 카테킨 화합물의 분리)

  • Jin, Yin-Zhe;Row, Kyung-Ho
    • Journal of the Korean Chemical Society
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    • v.51 no.2
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    • pp.165-170
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    • 2007
  • In this work, caffeine and some catechin compounds such as +C, EGC and EGCG were extracted from green tea using quercetin molecular imprinted polymers in solid-phase extraction. For synthesis of MIP, quercetin as the templates, MAA as the monomer, EGDMA as the crosslinker and AIBN as the initiator were used. For extraction of caffeine and catechin compounds from green tea, the solid-phase extractions of a load followed by wash and elution procedures were done with water, methanol and methanol:acetic acid=90:10 (vol.%) as the solvents, respectively. HPLC analysis (C18 column, 5 μm, 250×4.6 mm) with the mobile phase of methanol:water=40:60 (vol.%) at a flow rate of 0.5 ml/min was adopted for the quantitative determination. By solid-phase extraction, the resolutions of caffeine and some catechin compounds from green tea were increased. The quercetin-MIP had higher selectivity to +C compounds.

A Study on the Mass Transfer of Extraction Process by Use of Hollow Fiber Membrane Module (실관막 모듈을 이용한 추출공정의 물질전달에 관한 연구)

  • Kim, Young-Il;Jin, Do-Won;Kim, Jong-Hyun;Choi, Dai-Ung;Park, Dong-Won
    • Applied Chemistry for Engineering
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    • v.7 no.5
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    • pp.977-984
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    • 1996
  • Liquid-liquid extractions by use of hollow fiber membrane module are fast because of the large surface area per volume. In these membranes, the extractant and feed can be contacted at high speed and two flows are completely independent, so there are no problems with loading and channeling. In this paper, it was investigated the selectivities of extractants for extraction of heavy metals from aqueous solution into organic extractants by using the hollow fiber membrane. To identify the effect of distribution ratio on mass transfer in the membrane, we also compared the distribution ratio with mass transfer coefficient. From these experiments for the system with high distribution ratio, effect of the distribution ratio on mass transfer was weak compare with the low distribution ratio system in the hollow fiber membrane.

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A Study on the In-Situ Soil Vapor Extraction and Soil Flushing for the Remediation of the Petroleum Contaminated Site (유류로 오염된 토양 복원을 위한 토양가스추출 및 세척공정의 현장적용 연구)

  • Ko, Seok-Oh;Kwon, Soo-Youl;Yoo, Hee-Chan;Kang, Hee-Man;Lee, Ju-Goang
    • Journal of the Korean Society of Hazard Mitigation
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    • v.1 no.3 s.3
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    • pp.83-92
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    • 2001
  • Field investigations for subsurface soil and groundwater at a gas station showed that the site was severely contaminated and even petroleum compounds as free liquid state were observed. Pilot-scale soil flushing and soil vapor extraction process(SVE) were applied to evaluate the effectiveness of pollutants removal. Surfactant solution, Tween 80, was used to enhance the solubility of petroleum compounds and resulted in about 10 times increase on TPH(Total Petroleum Hydrocarbon) concentration. As for SVE method, maximum concentration of TPH and BTEX reached within 24 hours of extraction and then continuously decreased. Considerations on the groundwater level and the kinetic limitation for volatilization of contaminants have to be taken into account for the effective application of SVE process.

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Separation of Immunoglobulin G from Colostrum by Reverse Micelles of Cationic Surfactant (양이온 계면활성제로 형성된 역미셀을 이용한 초유 Immunoglobulin G의 분리)

  • 노경현;임지영
    • Food Science of Animal Resources
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    • v.24 no.1
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    • pp.80-85
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    • 2004
  • This study was carried out to separate immunoglobulin G(IgG) from colostrum using reverse micellar extraction of cationic surfactant and to suggest suitable extraction conditions. The reconstituted colostrum powder was solubilized into a reverse micellar phase containing CDAB(cetyldimethylethyl ammonium bromide) by mixing equal volume of the aqueous and organic phase with constant stirring. The solubilization of proteins from the aqueous to the organic phase was manipulated by pH and ionic strength of the aqueous phase and concentration of surfactant in the organic phase. Based on the SDS-PAGE and densitometry, about more than 90% of initial IgG was remained in the aqueous phase after reverse micellar extraction. Although the aqueous phase contained lactoferrin and bovine serum albumin as minor components, about 93% of the total protein was IgG. The efficient extraction was achieved by the reaction of sodium phosphate buffer(pH 8) containing 50 mM KCl and organic phase containing 100 mM CDAB. The separation of IgG using reverse micellar extraction was simple, highly efficient and easy to be scaled up.

Stability of Some Fried Foods Prepared with Oils Containing Rhus javanica $Linn{\acute{e}}$ Ethanol Extract with Several Synergists (붉나무 추출물과 몇가지 synergist를 첨가한 기름 튀김 식품의 저장 안정성 비교)

  • Shin, Dong-Hwa;Lee, Yun-Jae;Chang, Young-Sang;Kang, Woo-Suk
    • Korean Journal of Food Science and Technology
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    • v.24 no.6
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    • pp.547-551
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    • 1992
  • The antioxidative effects of Rhus javanica $Linn{\acute{e}}$ ethanol extract (RE) with or without some synergists were determined by measurement of POV and TBA of oil in ramyun and potato flake fried in palm oil or lard. RE with ascorbic acid and with tocopherol significantly increased the oxidative stability of ramyun. The results obtained from sensory evaluation were similar to those of POV and TBA values. RE with phosphoric acid and with ascorbic acid retarded the oxidation of potato flakes fried in palm oil or lard during storage.

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Anti-Inflammatory Effect of Germinated Mung Bean and Hairdye Applications (발아 녹두 추출물의 항염증 효능 및 염모제 응용)

  • Kim, Eun-Seok;Jeong, Noh-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.1
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    • pp.23-30
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    • 2014
  • In this study, a research of anti-inflammatory effect and application for cosmetics after extracting of germinated mung bean. Germinated mung bean extract was showed high NO constraining effect compared with $250{\mu}g/m{\ell}$ concentrated mung bean extract and germinated mung bean extract. Also shown is the highest nitrogen oxide inhibiting effect that using 1,3-butylene glycol when comparing the results of extraction conditions of a water-soluble, and extracted mung bean germinated for 5 days. The result of applying the germinated mung bean extract hairdye inflammatory reaction happens many showed nitrogen oxides higher inhibitory effect than that hair dye containing germinated mung bean extract is not included. Therefore, germinated mung bean extract is expected to the abirritant and anti-inflammatory agent as the material for cosmetic.

Management of Earthworm Casts using Pesticides and Tea Tree (Camellia oliefera) Extracts in Turfgrass of Golf Courses (골프장에서 차나무류 추출물과 화학 방제제를 이용한 지렁이 분변토 발생 관리)

  • Shin, Chong Chang;Kim, Jong Kyung;Choi, Sung Hwan;Jeon, Byungduk;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.5 no.1
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    • pp.35-41
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    • 2016
  • Earthworm cast is a harmful factor which reduces the uniformity of management of turfgrass and play quality in golf courses and playground. This research was conducted to carry out the efficacy of some pesticide (bifenthrin GR + clothianidin GR, ethoprophos GR, imidacloprid SC, picoxystrobin WG, thiophanate-methyl WP) and tee tree (Camellia oliefera) extracts against reducing earthworm cast in golf courses. All tested materials were acted reducing earthworm cast, thiophanate-methyl among them showed the highest effect reducing earthworm casts in golf courses. Tea tree extracts was occurred similar efficacy among tested chemical pesticides. However, pellet type byproduct of tea seed extract and liquid formulation of tea tree extract were better than granule formulation of them. Byproduct of tea seed and tea tree extract can be used for alternative biological management material for reducing earthworm cast in heavily casting occurrence area.

The Antioxidant and Skin Whitening Effect of Withania somnifera (Winter Cherry) (윈터체리 추출물의 항산화 및 미백 개선 효과)

  • Kim, Dae Yong;Kim, Mee Kyung;Kim, Bong-Woo
    • Journal of Food Hygiene and Safety
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    • v.30 no.3
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    • pp.258-264
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    • 2015
  • Withania somnifera has been used in folk medicine to treat various ailments for centuries. In this studies to investigate the whitening effect of Withania somnifera extracts as an active ingredient for whitening cosmetics, the antioxidant capacity and the effects of Withania somnifera extracts on melanogenesis in B16-F10 melanoma cells were identified. Withania somnifera extracts significantly reduced both tyrosinase activity and melanin content in a concentration-dependent manner. Furthermore, it was found that Withania somnifera extracts decreased ${\alpha}-MSH$ (melanocyte-stimulating hormone)-induced tyrosinase activity and MITF(microphthalmia associated transcription factor) protein expression. These data indicate that Withania somnifera extracts attenuate ${\alpha}$-MSH-stimulated melanin synthesis by modulating MITF expression and that they may be a useful therapeutic agent for treating hyperpigmentation and an ingredient of whitening cosmetics.

Studies on Xanthine Oxidase from Bovine Thyroid Glands -[Part 1] Purification and Substrate Specificity- (소의 갑상선에 있는 크산친 옥시다아제에 관한 연구 -[제1보] 효소의 정제와 기질특이성-)

  • Lee, Hyo-Sa
    • Applied Biological Chemistry
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    • v.21 no.2
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    • pp.112-118
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    • 1978
  • Xanthine oxidase from bovine thyroid glands was purified to apparent homogeneity when judged by analytical disc gel electrophoresis. The purification procedures include pancreatin digestion, butanol extraction, ammonium sulfate precipitation, calcium phosphate gel adsorption, ultrafiltration, calcium phosphate gel-cellulose column chromatography, gel filtration, preparative Sephadex G-25 column electrophoresis, and preparative polyacrylamide gel electrophoresis. The enzyme was enriched 1,000-fold. However, its specific activity was markedly low as compared with highly purified milk enzyme. Thyroidal xanthine oxidase exhibited a low specificity for substrates and electron acceptors. The kinetic properties of thyroid xanthine oxidase were found to be similar to those of the milk enzyme on the basis of Michaelis constants for common substrates.

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