• Title/Summary/Keyword: 저항성 탐색

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Identification of Leaf Blast Resistance Genes Derived from a Korean Weedy Rice, Ganghwaaengmi 11 (잡초성벼인 강화앵미11 유래 잎도열병 저항성 유전자 탐색)

  • Suh, Jung-Pil;Cho, Young-Chan;Kim, Jeong-Ju;Shin, Young-Seop;Yang, Chang-Ihn;Roh, Jae-Hwan;Kim, Yeon-Gyu
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.390-396
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    • 2010
  • A weedy rice, Ganghwaaengmi 11, shows high level of leaf blast resistance. The chromosomal number and locations of genes conferring the leaf blast resistance were detected by QTL (quantitative trait loci) analysis using SSR markers in the 120 RILs (recombinant inbred lines) derived from the cross between Nagdongbyeo and Ganghwaaengmi 11. Ganghwaaengmi 11 expressed compatibility with 20 of the 45 inoculated blast isolates, in contrast to Nagdongbyeo with 44 compatible isolates. To identify QTLs affecting partial resistance, RILs were assessed in upland blast nursery in three regions and inoculated with selected nine blast isolates. QTLs for resistance to blast isolates were identified on chromosomes 7, 11 and 12. Three QTLs associated with blast resistance in nursery test at three regions were also detected on chromosomes 7, 11 and 12. The QTL commonly detected on chromosome 12 was only increased blast resistance by Ganghwaaengmi 11 allele. This QTL accounted for 60.3~78.6% of the phenotypic variation in the blast nursery test. OSR32 and RM101 markers tightly linked to QTL for blast resistance on chromosome 12 might be useful for marker-assisted selection (MAS) and gene pyramiding to improve the blast resistance of japonica rice.

Identification of a Major QTL, qSTV11SG, Associated with Resistance to Rice Stripe Virus Disease Originated from Shingwangbyeo in Rice (Oryza Sativa L.) (신광벼 유래의 벼 줄무늬잎마름병 저항성 주동 QTL qSTV11SG탐색)

  • Kwak, Do-Yeon;Lee, Bong-Chun;Choi, Ilyoung;Yeo, Un-Sang;Cho, Jun-Hyun;Lee, Ji-Yoon;Song, You-Chun;Yun, Yeong-Nam;Park, Dong-Soo;Kang, Hang-Won;Nam, Min-Hee;Lee, Jong-Hee
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.464-469
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    • 2011
  • Virus diseases often cause serious damage to rice production in Asia. The lack of information on virus resistance genes has been a major obstacle for the breeding of resistant varieties. In order to identify DNA marker associated with resistance against rice stripe virus (RSV), the quantitative trait locus (QTL) was carried out using advanced backcross population developed from a cross between RSV-resistant tongil type cultivar Shinkwang and susceptible japonica cultivar Ilpum. A RSV resistance QTL $qSTV11^{SG}$ explaining 44.2% of the phenotypic variation was identified on chromosome 11 of Tongil type rice cultivar 'Shingwang'. $qSTV11^{SG}$ was tightly linked to DNA marker RM6897. The RM6897 divided as resistance type allele and susceptible type alleles. Twenty seven resistant varieties showed the resistant-type allele and 23 susceptible varieties were susceptible-type allele to the marker of RM6897. This results and the molecular markers presented here may be useful in rice breeding for improving RSV resistance in japonica rice.

Mapping and Race Specific Reaction of the Resistance Gene Pi45(t) in Rice (벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성)

  • Kim, Dong-Min;Ju, Hong-Guang;Yang, Paul;Han, Seong-Sook;Roh, Jae-Hwan;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.42-49
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    • 2011
  • QTL analysis for blast resistance was carried out using 140 $BC_3F_3$ lines derived from a cross between Ilpum as a recurrent parent and Moroberekan as a donor parent. 140 $BC_3F_3$ lines with the parents were inoculated with nine blast isolates. To identify QTLs for resistance to nine blast isolates, 134 SSR markers showing polymorphisms between the parents were genotyped for the 140 $BC_3F_3$ lines. A total of 17 resistance QTLs to nine isolates were detected on chromosomes 2, 3, 4, 6, 7, 9 and 10. The phenotypic variance explained by each QTL ranged from 8.2% to 26.4%. The Moroberekan alleles contributed the positive effect at these 17 QTL loci. In a previous study, the QTL, Pi45(t) for durable resistance to blast was identified using a sequential planting method. To know the relationship between Pi45(t) and the isolate-specific resistance gene, an $F_2$ population was developed from a cross between Ilpum and an introgression line harboring Pi45(t). $F_3$ lines segregating for the Pi45(t) were inoculated to three isolates. $F_3$ lines from the $F_2$ plants with the Moroberekan segment at the target region showed resistance to two isolates. This result seems to indicate that the Pi45(t) and the isolate-specific resistance gene are tightly linked or the resistance is controlled by the same gene(s). The markers linked to genes controlling blast resistance would be useful in developing blast resistance lines in the breeding program.

Determinant Factors of Innovation Resistance of Social Media (소셜미디어 혁신저항 결정요인에 관한 연구)

  • Jeong, Hwa-Seob
    • The Journal of the Korea Contents Association
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    • v.13 no.6
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    • pp.158-166
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    • 2013
  • This study gave attention to the people who has resistance of the social media that tens to be the use as innovations. Therefore, this study examined the determinant factors of psychological resistance of the social media such as Twitter focused on students as none-users of social media. Total of 268 none-users participated in this study. The results were as follows. First, relative advantage influenced negatively on innovation resistance. Second, perceived complexity influenced significantly not on innovation resistance. Third, perceived risk influenced positively on innovation resistance. Therefore, social media related to social relationship should improve relative advantage, the other way, decrease perceived risk such as defamation and personal attack.

Identification of Quantitative Trait Loci for Resistance to Soybean Cyst Nematode Race 5 (콩 Cyst 선충 Race 5에 대한 저항성 QTL 탐색)

  • Choi, In-Soo;Kim, Yong-Chul;Kim, Sung-Man;Lee, Chung-Yeol;Park, Hyean-Cheal;Halina T. Skorupska
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.712-721
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    • 1997
  • The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E02$^3$ on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E02$^3$ and W03, pK418C and pK418E$_1$) and one unlinked RAPD marker, G10$^1$ were used for multiple regression analysis. Marker combination which was composed of 4 markers, E02$^3$, G10$^1$, W03, and pK418E$_1$, explained the highest amount of phenotypic variation by SCN (35.2%). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.

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Selection of a Susceptible Line (Susceptible to Pectobacterium 1, Atstp1) to Soft-rot Disease in T-DNA Insertion Mutants Pool of Arabidopsis (무름병에 감수성인 애기장대 돌연변이체 Atstp1 선발)

  • Choi, Chang-Hyun;Kim, Min-Gab;Ahn, Il-Pyung;Park, Sang-Ryeol;Bae, Shin-Chul;Hwang, Duk-Ju
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.312-315
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    • 2010
  • Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc.

Search for Multiple Disease Resistance in Groundnut (땅콩의 복합병 저항성 탐색)

  • Mathur, Sneh;Doshi, Anila
    • Korean journal of applied entomology
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    • v.26 no.4 s.73
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    • pp.257-259
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    • 1987
  • One thirty one varieties of Groundnut were screened for search of multiple disease resistance against rust caused by Puccinia arachidis speg. and leaf spots caused by Cereospora arachidis Hori and Phaeoisariopsis personata . Out of these, 7 were resistant, and 11 were moderatly resistant. Percent disease severity and its' effect on yield was assessed.

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