Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Selection of a Susceptible Line (Susceptible to Pectobacterium 1, Atstp1) to Soft-rot Disease in T-DNA Insertion Mutants Pool of Arabidopsis

무름병에 감수성인 애기장대 돌연변이체 Atstp1 선발

  • 최창현 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 김민갑 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 안일평 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 박상렬 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 배신철 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과) ;
  • 황덕주 (농촌진흥청 국립농업과학원 농업생명자원부 신작물개발과)
  • Received : 2010.11.08
  • Accepted : 2010.11.20
  • Published : 2010.12.01
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Abstract

Pectobacterium carotovorum subsp. carotovorum (Pcc) causes soft rot disease in various plants. Although many studies about Pcc have been going on, little is known yet about the defense genes from plants. To identify defense associated genes in response to Pcc, we screened about 20 thousand Arabidopsis T-DNA knock out lines by inoculation with Pcc. We obtained a line (Atspt1) showing more susceptible symptom compared to WT (Col-0) on 1 day after the inoculation of Pcc on leaves of Arabidopsis with toothpicks. In this study, we optimized the system to select resistant and susceptible lines to Pcc from T-DNA inserted pool of Arabidopsis and expect the system and Atspt1 might be used for molecular breeding to produce resistant vegetables against Pcc.

본 연구는 애기장대에서 무름병에 대한 저항성 유전자를 탐색하고자 2만여개의 T-DNA 삽입 돌연변이군을 이용하여 Pcc에 대한 스크리닝을 수행하고 이 방법을 소개한 연구다. 1차 선발을 통하여 15개의 저항성 line과 20개의 감수성 line을 선발하였으며, 이로부터 2차 선발하여 3개의 저항성 line과 4개의 감수성 line을 선발하였고, 최종적으로 3차 선발을 통하여 1개의 감수성 line (Atstp1)을 선발할 수 있었다. 현재 Atstp1을 이용해 flanking sequencing 하여 유전자를 탐색하고 있으며, 앞으로 클로닝을 통하여 다양한 무름병 저항성 식물 개발에 유용하게 이용될 것으로 기대한다.

Keywords

References

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