• Korean Journal of Weed Science
• /
• v.11 no.3
• /
• pp.219-223
• /
• 1991

Effects of media and low temperature on germination of weeds were examined to get basic information for establishing weed control methods. Soil and agar(0.4%) was good media for germination of weeds at laboratory. Germination was improved by placing weed seeds at 5$^{\circ}C$ low temperature for 10-20 days. Germination was improved by storing weed seeds in water absorbed gauge and in vinylbag at 5$^{\circ}C$ low temperature for 30-40 days. Germination was increased by burial of weed seeds at 10cm of soil depth for 30-60 days.

• Korean Journal of Food Science and Technology
• /
• v.41 no.3
• /
• pp.284-291
• /
• 2009

This study was conducted to characterize the enhanced antioxidant and anticancer activities of Berberis koreana bark following a low temperature and high pressure extraction process. The results indicate that the B. koreana bark extracted as described showed a 93% increase in DPPH radical scavenging activity. Inhibition activity of xanthine oxidase was highest by this extraction process. In addition the growth of human lung cancer cells (A549), human stomach cancer cells (AGS), human breast cancer cells (MCF-7) and human liver cancer cells (Hep3B) were inhibited by 70.8%, 86.2%, 84.3% and 62.5% respectively. These data indicate that this low temperature and high pressure extraction technique results in the efficient extraction of bioactive compounds from rigid plant materials. This process could also be combined with other techniques to improve extraction yields and identify new biologically active substances from relatively hard plants.

• Korean Journal of Plant Tissue Culture
• /
• v.21 no.1
• /
• pp.15-22
• /
• 1994

To understand the molecular mechanism of hardening process in somatic embryo development and artificial seed germination in celery (Apium graveolens L.), the changes of protein synthesis by ABA or cold teatment at early globular stage were examined. Protein content and nitrate reductase activity in ABA- or cold-treated somatic embryo and seedlings were higher than that in unheated ones. The protein content and nitrate reductase activity were more prominent in somatic embryos than in seedlings. From two-dimensional electrophoresis, several protein spots specific to ABA or cold treatment were identified: 30 KD, 32 KD, 171 KD and 205 KD at heart-shaped stage; and 29 KD, 33 KD, 37 KD, 38 KD, 41 KD, 55 KD, 66 KD, and 110 KD at cotyledonary stage were the most specifically synthesized However the synthesis of certain polypeptides were repressed at heart-shaped or cotyledonary stage: 42 KD, 44 KD, 59 KD, 64 KD, 101 KD, 104 KD, and 190 KD at heart-shaped stage; and 29 KD and 116 KD at cotyledonary stage. The protein pattern changes by ABA or cold treatment occurred simultaneously and mainly in acid-soluble proteins during somatic embryo development and artificial seed germination. Therefore it is suggested that the metabolic changes for adaptation to environmental change occur during somatic embryo development and the germination and growth of seedling from embryo.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.22 no.2
• /
• pp.23-26
• /
• 1977

In order to test cold tolerance of rice plants 16 varieties were treated at day/night temperature of 10/5$^{\circ}C$ at the 2nd, 4th, and 6th leaf stages. Indica$\times$japonica varieties were more sensitive to low temperature than a japonica variety. Among indica$\times$japonica varieties, "Tongil", "Suweon ＃251", and "Suweon ＃277" were less sensitive to low temperature compared to other indica$\times$japonica varieties tested. The 4th leaf stage was more sensitive to low temperature than 2nd and 6th leaf stages. than 2nd and 6th leaf stages.

• Korean Journal of Poultry Science
• /
• v.17 no.2
• /
• pp.127-133
• /
• 1990

This study was undertaken to find out the effect of heating time and temperature, pH and NaCl concentration on the heat stability of egg albumen gel during heat treatment. With the transient decrease at 110-$130^{\circ}C$, hardness of heat-set albumen gel was increased as the heating temperature increased. The cohesiveness showed similar trend as well. The lightness was decreased while the yellowness was increased as the heating time and temperature increased. Heat-set albumen gel showed maximum hardness at pH 4.5-5.0 and pH 9.0 High heat treatment($120^{\circ}C$, 30min) showed higher hardness at alkaline range compared to low heat treatment($96^{\circ}C$, 30min.). Color of the albumen gel was relatively dark at acidic range and bright at alkaline range. High heat treatment caused darker albumen gel at alkaline range and brighter albumen gel at acidic range. The addition of NaCl increased hardness and cohesiveness of the albumen gel and improved the lightness after high heat treatment regardless of NaCl concentration.

• Journal of Food Hygiene and Safety
• /
• v.32 no.1
• /
• pp.64-69
• /
• 2017

This study was conducted to investigate the effect of cold plasma combined with UV-C irradiation against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes on lettuce. E. coli O157:H7, S. Typhimurium, and L. monocytogenes, corresponding to approximately 5.82, 5.09, 5.65 log CFU/g, were inoculated on lettuce, respectively. Then, the lettuce was treated with cold plasma, UV-C and combination (cold plasma + UV-C), respectively. The treated lettuce was stored for 9 days at $4^{\circ}C$ for microbiological analysis and sensory evaluation. Cold plasma reduced the populations of E. coli O157:H7, S. Typhimurium, and L. monocytogenes by 0.26, 0.65, and 0.93 log CFU/g, respectively. Each microorganism were reduced by 0.87, 0.88, and 1.14 log CFU/g after UV-C treatment. And, the combined treatment that was treated by cold plasma after UV-C treatment reduced the populations of inoculated microorganisms by 1.44, 2.70, 1.62 log CFU/g, respectively. The all treatment significantly (p < 0.05) reduced the populations of all inoculated bacteria compared to untreated lettuce. UV-C combined with cold plasma was the most effective for reducing the pathogenic bacteria on lettuce, by showing log-reductions of ${\geq}2.0\;log\;CFU/g$. All treatment was not significantly different until 6 day storage compared to control group in terms of appearance, texture and overall acceptability. Therefore, the combined treatment will be an effective intervention method to control the bacteria on lettuce.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.28 no.2
• /
• pp.189-194
• /
• 1983

The experiment was carried out to know the ripeness effect of Gemgang when ABA and BA were sprayed at the heading stage. ABA promoted the stomatal movement, BA kept plant from senescence. Percent of filled grain, grain weight, photosynthesis, content of chlorophyll, transpiration and content of ATP were measured at 1-week interval from 2-weeks after heading.

• Journal of Bio-Environment Control
• /
• v.32 no.2
• /
• pp.157-164
• /
• 2023

Codonopsis lanceolata (S. et Z.) Trautv. is mainly cultivated in Korea and China as a medicinal crop. C. lanceolata is difficult to produce plug seedlings in the summer, because C. lanceolata has a low germination rate and is vulnerable to high temperatures. Cold treatment is effective in breaking dormancy of seeds and increasing the germination rate. Shading cultivation can control the solar irradiance received by plants and reduce the damage by high temperatures and strong light. This study was conducted to examine the appropriate cold treatment period for the improving germination of C. lanceolata, and shading level during the summer seedling period. Cold treatment experiments were conducted for 0 (control), 1, 2, 3, and 4 weeks at 4℃ before sowing. In the shading experiment, C. lanceolata was grown for 45 days with 0 (non-treatment), 45, 75% shading levels. Cold treatment for one week significantly improved the germination energy. The plant height, leaf area, and fresh and dry weights of C. lanceolata seedlings were significantly increased under the 45% shading level. Total root length, root surface area, and the number of root tips were significantly higher in shading treatment (45 and 75%) than in non-treatment. The C. lanceolata seedling's compactness and Dickson's quality index were the highest at 45% shading level. Therefore, these results recommended sowing C. lanceolata after cold treatment for one week at 4℃, and 45% shading level could stably culture C. lanceolata plug seedlings during the high temperature period.

• Journal of Life Science
• /
• v.13 no.1
• /
• pp.9-14
• /
• 2003

Seed germinability might be highly related to seedling establishment. The experiments were carried out to evaluate the effect of presowing treatments of aging, priming, $GA_3$ , prechilling and water imbibition, different temperatures and light quality during germination on germination of gourd (Lagenaria siceraria Standl.) seeds. Aging treatment with different temperatures and periods was done by the accelerated aging method. Priming using KNO$_3$ and $GA_3$ treatment for 24 hours were done at 100 mM and at 0.01 mM before a week prechilling. The germination tests using a week prechilled seeds were done at 10, 20 and $30^{\circ}C$ on the condition of darkness, and also with blue, red, far-red light and darkness as light quality treatments. FR-yongjadaemok and FR-kunghap were used as test cultivars and their daily germination rates were measured at treatment levels. Germination rates were reduced in the seeds aged at $45^{\circ}C$ for 6 days. Prechilling showed the highest and fastest rate among the four presowing treatments but the two other presowing treatments had similar or less rates than water imbibition. Prechilling done before sowing enhanced the low vigor seeds, and accelerated the germination of cv. FR-kunghap at 20 to $30^{\circ}C$ and with blue, red light or darkness during germination although far-red light inhibited their germination.

• Journal of Sericultural and Entomological Science
• /
• v.18 no.1
• /
• pp.7-10
• /
• 1976

This experiments were carried out to know hatching power on treatments of cold temperature and hydrochioric acid Iron Late January to Early February. Used silkworm variety was Jam 103${\times}$ Jam 104 laying in Spring. The results may be summarized as follows: 1. The resting of silkworm eggs activated to Late January, to longer cold treatment was, the more effects of activating increased completely. So activated eggs were possible to incubation. 2. How to the light or short hours of hycrochloric acid were compare with heavy or long each other, it was inclined that the former seems to be worse than the latter. 3. On the interaction of cold treatments and hydrochloric acid, in case of short cold treatment, hatching power was better effect all treatments of hydrochloric acid than control, but no difference between specific gravity or treatment hours. On tile contrary, in case of more longer of cold treatments, hatching power depended not only upon the specific gravity, but also upon the treatment hours.