Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Combined Effect of Cold Plasma and UV-C Against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on Fresh-cut Lettuce

양상추에 인위접종된 Escherichia coli O157:H7, Salmonella Typhimurium과 Listeria monocytogenes에 대한 저온 플라즈마와 UV-C의 살균 효과

  • 성지영 (국민대학교 자연과학대학 식품영양학과) ;
  • 박미정 (국민대학교 자연과학대학 식품영양학과) ;
  • 권기현 (한국식품연구원) ;
  • 오세욱 (국민대학교 자연과학대학 식품영양학과)
  • Received : 2016.11.01
  • Accepted : 2016.11.08
  • Published : 2017.02.28
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Abstract

This study was conducted to investigate the effect of cold plasma combined with UV-C irradiation against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes on lettuce. E. coli O157:H7, S. Typhimurium, and L. monocytogenes, corresponding to approximately 5.82, 5.09, 5.65 log CFU/g, were inoculated on lettuce, respectively. Then, the lettuce was treated with cold plasma, UV-C and combination (cold plasma + UV-C), respectively. The treated lettuce was stored for 9 days at $4^{\circ}C$ for microbiological analysis and sensory evaluation. Cold plasma reduced the populations of E. coli O157:H7, S. Typhimurium, and L. monocytogenes by 0.26, 0.65, and 0.93 log CFU/g, respectively. Each microorganism were reduced by 0.87, 0.88, and 1.14 log CFU/g after UV-C treatment. And, the combined treatment that was treated by cold plasma after UV-C treatment reduced the populations of inoculated microorganisms by 1.44, 2.70, 1.62 log CFU/g, respectively. The all treatment significantly (p < 0.05) reduced the populations of all inoculated bacteria compared to untreated lettuce. UV-C combined with cold plasma was the most effective for reducing the pathogenic bacteria on lettuce, by showing log-reductions of ${\geq}2.0\;log\;CFU/g$. All treatment was not significantly different until 6 day storage compared to control group in terms of appearance, texture and overall acceptability. Therefore, the combined treatment will be an effective intervention method to control the bacteria on lettuce.

본 연구에서는 양상추에 접종된 E. coli O157:H7, S. Typhimurium과 L. monocytogene에 대하여 저온 플라즈마와 UV-C 단독처리 및 병행처리 효과를 측정하였다. E. coli O157:H7, S. Typhimurium, L. monocytogenes는 양상추에 초기 농도가 5.82, 5.09, 5.65 log CFU/g이 되도록 각각 접종하였다. 저온 플라즈마와 UV-C를 처리된 양상추는 $4^{\circ}C$에서 9일간 보관하며 미생물학적 분석과 관능평가를 실시하였다. 저온 플라즈마 처리는 E. coli O157:H7, S. Typhimurium, L. monocytogenes의 개체 수를 각각 0.26, 0.65, 0.93 log CFU/g 수준으로 감소시켰다. 또한, UV-C 처리 시 각각 0.87, 0.88, 1.14 log CFU/g 수준으로 감소되었다. 또한 UV-C 처리 후 저온 플라즈마를 처리한 병행처리에서는 각각 1.44, 2.70, 1.62 log CFU/g 수준으로 감소하였다. 저온 플라즈마와 UV-C 단독처리 보다는 병행처리가 좀 더 효과적으로 균을 저감하는 것으로 판단되었다. 관능평가 결과는 외관, 질감, 전체적인 수용도 면에서 대조구와 비교하였을 때, 보관 6일까지 유의적인 차이가 없었다. 따라서 저온 플라즈마와 UV-C 병행처리는 양상추에 존재하는 균을 저감하기 위한 효과적인 기술로 활용될 수 있을 것으로 생각되었다.

Keywords

References

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