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Appropriate Cold Treatment Periods and Shading Levels on Codonopsis lanceolata for Plug Seedling Production in Summer Season

더덕 플러그묘의 하절기 생산을 위한 적정 저온처리 기간과 차광 수준

  • Eun Won Park (Division of Applied Life Science, Graduate School of Gyeongsang National University) ;
  • Jeong Hun Hwang (Division of Applied Life Science, Graduate School of Gyeongsang National University) ;
  • Hee Sung Hwang (Division of Crop Science, Graduate School of Gyeongsang National University) ;
  • Hyeon Woo Jeong (Division of Applied Life Science, Graduate School of Gyeongsang National University) ;
  • So Yeong Hwang (Division of Applied Life Science, Graduate School of Gyeongsang National University) ;
  • Jin Yu (Division of Applied Life Science, Graduate School of Gyeongsang National University) ;
  • Seung Jae Hwang (Department of Agricultural Plant Science, College of Agriculture & Life Sciences, Gyeongsang National University)
  • 박은원 (경상국립대학교 응용생명과학부) ;
  • 황정훈 (경상국립대학교 응용생명과학부) ;
  • 황희성 (경상국립대학교 작물생산과학부) ;
  • 정현우 (경상국립대학교 응용생명과학부) ;
  • 황소영 (경상국립대학교 응용생명과학부) ;
  • 유진 (경상국립대학교 응용생명과학부) ;
  • 황승재 (경상국립대학교 농업생명과학대학 농업식물과학과)
  • Received : 2023.04.06
  • Accepted : 2023.04.24
  • Published : 2023.04.30

Abstract

Codonopsis lanceolata (S. et Z.) Trautv. is mainly cultivated in Korea and China as a medicinal crop. C. lanceolata is difficult to produce plug seedlings in the summer, because C. lanceolata has a low germination rate and is vulnerable to high temperatures. Cold treatment is effective in breaking dormancy of seeds and increasing the germination rate. Shading cultivation can control the solar irradiance received by plants and reduce the damage by high temperatures and strong light. This study was conducted to examine the appropriate cold treatment period for the improving germination of C. lanceolata, and shading level during the summer seedling period. Cold treatment experiments were conducted for 0 (control), 1, 2, 3, and 4 weeks at 4℃ before sowing. In the shading experiment, C. lanceolata was grown for 45 days with 0 (non-treatment), 45, 75% shading levels. Cold treatment for one week significantly improved the germination energy. The plant height, leaf area, and fresh and dry weights of C. lanceolata seedlings were significantly increased under the 45% shading level. Total root length, root surface area, and the number of root tips were significantly higher in shading treatment (45 and 75%) than in non-treatment. The C. lanceolata seedling's compactness and Dickson's quality index were the highest at 45% shading level. Therefore, these results recommended sowing C. lanceolata after cold treatment for one week at 4℃, and 45% shading level could stably culture C. lanceolata plug seedlings during the high temperature period.

더덕은 한국과 중국에서 주로 재배되는 약용작물로 낮은 발아율과 고온에 취약한 문제점을 지니고 있어 하절기 육묘에 어려움이 있다. 저온처리는 종자의 휴면을 타파하고 발아율을 향상시키는데 효과적이다. 차광재배는 식물체가 받는 태양광을 조절함으로써 고온과 강한 광에 의한 피해를 줄일 수 있다. 본 연구는 더덕의 발아를 위한 적정 저온처리 기간과 육묘기간 중 차광 수준을 조사하기 위해 수행되었다. 저온처리는 4℃에서 0(control), 1, 2, 3, 그리고 4주간 수행되었다. 차광 시험은 더덕을 0(무처리), 45, 그리고 75% 차광 수준에서 45일간 육묘하였다. 일주일간의 저온처리는 더덕의 발아세를 유의하게 향상시켰다. 45% 차광처리에서 더덕 묘의 초장, 엽면적, 그리고 생체중과 건물중은 유의적으로 증가되었다. 총 뿌리 길이, 뿌리의 표면적과 근단 수 등의 지하부 생육은 45%와 75% 차광처리에서 무처리에 비해 향상되었다. 묘소질 지표인 충실도와 DQI는 45% 차광처리에서 가장 우수하였다. 따라서 더덕은 4℃에서 일주일간 저온처리를 거친 후 파종하고, 하절기 육묘 시 45% 차광처리를 통해 안정적인 육묘가 가능할 것이다.

Keywords

Acknowledgement

이 연구는 2020년도 경상국립대학교 연구년제 연구교수 연구지원비에 의하여 수행되었음.

References

  1. Ahn S.D., and C.Y. Yu 1996, Effect of the short-time growing seedling for bolting control in Angelica gigas Nakai. Korean J Plant Res 9:157-164. (in Korean)
  2. Baskin C.C., and J.M. Baskin 2014, Seeds: Ecology, biogeography, and evolution of dormancy and germination, Ed 2. Elsevier/Academic Press, San Diego, CA, USA, pp 39-55.
  3. Baskin J.M., and C.C. Baskin 2004, A classification system for seed dormancy. Seed Sci Res 14:1-16. doi:10.1079/SSR2003150
  4. Beadle C.L. 1993, Growth analysis. In DO Hall, ed, Photosynthesis and production in a changing environment: A field and laboratory manual. Chapman & Hall, London, UK, pp 36-46.
  5. Dickson A., A.L. Leaf, and J.F. Hosner 1960, Quality appraisal of white spruce and white pine seedling stock in nurseries. For Chron 36:10-13. doi:10.5558/tfc36010-1
  6. Ellis R.H., and E.H. Roberts 1981, The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9:373-409.
  7. Farooq M., S.M.A. Basra, N. Ahmad, and K. Hafeez 2005, Thermal hardening: A new seed vigor enhancement tool in rice. J Integr Plant Biol 47:187-193. doi:10.1111/j.1744-7909.2005.00031.x
  8. Ghimire B.K., C.M. Shin, C.H. Li, I.M. Chung, D.W. Lee, H.Y. Kim N.Y. Kim, J.D. Lim, J.K. Kim, M.J. Kim, D.H. Cho, S.J. Lee, and C.Y. Yu 2006, Effect of gibberellin and light on germination of seeds in Codonopsis lanceolata Benth. Korean J Med Crop Sci 14:303-306.
  9. Grime J.P., and D.W. Jeffrey 1965, Seedling establishment in vertical gradients of sunlight. J Ecol 53:621-642. doi:10.2307/2257624
  10. Heins R.D., H.B. Pemberton, and H.F. Wilkins 1982, The influence of light on lily (Lilium longiflorum Thunb.). I. influence of light intensity on plant development. J Am Soc Hortic Sci 107:330-335. https://doi.org/10.21273/JASHS.107.2.330
  11. Hossen M.J., M.Y. Kim, J.H. Kim, and J.Y. Cho 2016, Codonopsis lanceolata: A review of its therapeutic potentials. Phytother Res 30:347-356. doi:10.1002/ptr.5553
  12. Im D.H., J.H. Nam, J.H. Kim, M.J. Lee, and I.R. Rho 2020, Effect of pelleting treatment on seed germination in Adenophora triphylla. Korean J Med Crop Sci 28:128-135. (in Korean) doi:10.7783/KJMCS.2020.28.2.128
  13. Jang I.B., J.W. Moon, J. Yu, I.B. Jang, S.J. Suh, and C.H. Chun 2019, Analysis of microclimate responses and high-temperature injury in ginseng as affected by shading. Korean J Med Crop Sci 27:278-283. (in Korean) doi:10.7783/KJMCS.2019.27.4.278
  14. Jeong B.R. 1998, Technology and environment management for the production of plug transplants of flower crops. Korean J Hortic Sci Technol 16:282-286. (in
  15. Jeong B.R., S.J. Hwang, and N.J. Kang 2016, Plug seedling. GSpress, Jinju, Korea, pp 16-20. (in Korean)
  16. Kami C., S. Lorrain, P. Hornitschek, and C. Fankhauser 2010, Light-regulated plant growth and development. Curr Top Dev Biol 91:29-66. doi:10.1016/S0070-2153(10)91002-8
  17. Kim H.H., I. Miyajima, S.R. Lee, and C.K. Yoon 1997, Effect of temperature and light intensity on growth and yield of Codonopsis lanceolata. Korean J Plant Res 10:392-396. (in Korean)
  18. Lee C.Y. 2002, Main factors affecting on photosynthesis under shading condition of Codonopsis lanceolata Trautv. Korean J Med Crop Sci 10:409-404. (in Korean)
  19. Lee C.Y., and J.Y. Won 2007, Effects of shading treatments on photosynthetic rate and growth in Codonopsis lanceolata Trautv. Korean J Med Crop Sci 15:152-156. (in Korean)
  20. Lee J.W., I.H. Jo, J.U. Kim, C.E. Hong, Y.C. Kim, D.H. Kim, and Y.D. Park 2018, Improvement of seed dehiscence and germination in ginseng by stratification, gibberellin, and/or kinetin treatments. Hortic Environ Biotechnol 59:293-301. doi:10.1007/s13580-018-0039-6
  21. Lee K.T., J. Choi, W.T. Jung, J.H. Nam, H.J. Jung, and H.J. Park 2002, Structure of a new echinocystic acid bisdesmoside isolated from Codonopsis lanceolata roots and the cytotoxic activity of prosapogenins. J Agric Food Chem 50:4190-4193. doi:10.1021/jf011647l
  22. Lee S.R., E.S. Yoon, H.H. Kim, Y.S. Lee, and Y. Motoda 1992, The effect of components and yield with different temperature in Codonopsis lanceolata Benth. et Hook. fil. J Orient Bot Res 5:11-23. (in Korean)
  23. Nonogaki H., G.W. Bassel, and J.D. Bewley 2010, Germinationstill a mystery. Plant Sci 179:574-581. doi:10.1016/j.plantsci.2010.02.010
  24. Park K.W., G.P. Lee, K.W. Park, and J.C. Jeong 1998, Seed morphology of thirty Korean wild green species and effect of seed stratification on germination. J Korean Soc Hortic Sci 39:129-134. (in Korean)
  25. Park S.K., D.A. Rhee, and K.W. Song 1972, Study on Codonopsis lanceolata cultivation under shading condition. J Korean Soc Hortic Sci 11:25-28. (in Korean)
  26. Powles S.B., and C.B. Osmond 1978, Inhibition of the capacity and efficiency of photosynthesis in bean leaflets illuminated in a CO2-free atmosphere at low oxygen: a possible role for photorespiration. Aust J Plant Physiol 5:619-629. https://doi.org/10.1071/PP9780619
  27. Rural Development Administration (RDA) 2019, Medicinal crops. RDA, Jeonju, Korea, pp 140-149. (in Korean)
  28. Ryu S.H., Y.H. Rhie, S.Y. Lee, C.H. Ko, J.H. Lee, H.J. Lee, and K.C. Lee 2017, Effect of after-ripening, cold stratification, and GA3 treatment on Lychnis wilfordii (Regel) Maxim seed germination. Hortic Sci Technol 35:525-533. (in Korean) doi:10.12972/kjhst.20170057
  29. Schwienbacher E., J.A. Navarro-Cano, G. Neuner, and B. Erschbamer 2011, Seed dormancy in alpine species. Flora 206:845-856. doi:10.1016/j.flora.2011.05.001
  30. Seo H.T., B.K. Choi, Y.G. Moon, S.W. Kim, K.D. Park, and S.B. Kwon 2018, Effect of light conditions and wet cold treatments on seed germination in several wild vegetables. J Agric Life Environ Sci 30:64-72. (in Korean) doi:10.12972/jales.20180007
  31. Sonobe R., T. Sano, and H. Horie 2018, Using spectral reflectance to estimate leaf chlorophyll content of tea with shading treatments. Biosyst Eng 175:168-182. doi:10.1016/j.biosystemseng.2018.09.018
  32. Tang D.S., M. Hamayun, Y.M. Ko, Y.P. Zhang, S.M. Kang, and I.J. Lee 2008, Role of red light, temperature, stratification and nitrogen in breaking seed dormancy of Chenopodium album L.. J Crop Sci Biotechnol 11:199-204.
  33. Um I.S., M.J. Lee, J.H. Nam, and I.R. Rho 2019, Effects of additional fertilization after cultivating green manure crops on the growth and saponin content of Codonopsis lanceolata Trautv. Korean J Med Crop Sci 27:1-8. (in Korean) doi:10.7783/KJMCS.2019.27.1.1
  34. Woodstock L.W. 1988, Seed imbibition: a critical period for successful germination. J Seed Technol 12:1-15.
  35. Yamauchi Y., M. Ogawa, A. Kuwahara, A. Hanada, Y. Kamiya, and S. Yamaguchi 2004, Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. Plant Cell 16:367-378. doi:10.1105/tpc.018143
  36. Yang L.E., D.L. Peng, Z.M. Li, L. Huang, J. Yang, and H. Sun 2020, Cold stratification, temperature, light, GA3, and KNO3 effects on seed germination of Primula beesiana from Yunnan, China. Plant Divers 42:168-173. doi:10.1016/j.pld.2020.01.003