• Title/Summary/Keyword: 유사성 탐색

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Anlaysis of Eukaryotic Sequence Pattern using GenScan (GenScan을 이용한 진핵생물의 서열 패턴 분석)

  • Jung, Yong-Gyu;Lim, I-Suel;Cha, Byung-Heun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.11 no.4
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    • pp.113-118
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    • 2011
  • Sequence homology analysis in the substances in the phenomenon of life is to create database by sorting and indexing and to demonstrate the usefulness of informatics. In this paper, Markov models are used in GenScan program to convert the pattern of complex eukaryotic protein sequences. It becomes impossible to navigate the minimum distance, complexity increases exponentially as the exact calculation. It is used scorecard in amino acid substitutions between similar amino acid substitutions to have a differential effect score, and is applied the Markov models sophisticated concealment of the transition probability model. As providing superior method to translate sequences homologous sequences in analysis using blast p, Markov models. is secreted protein structure of sequence translations.

An Use of Dilemma Episodes in Science Teacher Education (딜레마 일화를 활용한 과학 교사 교육)

  • Yoon, Hye-Gyoung
    • Journal of The Korean Association For Science Education
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    • v.25 no.2
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    • pp.98-110
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    • 2005
  • This study aims to explore the usefulness and the way of practical use of dilemma episodes in science teacher education by the responses of teachers on the given dilemma episodes and connected discussions. The research based on the idea of constructivist teacher education, the assumption that dilemmas can provide teachers with an open investigation context, an understanding of nature of science education. The dilemmas episodes in Wallace and Louden(2002) were introduced and discussed in order during graduate course to 11 elementary teachers. Perception on the reality and importance of given dilemmas differed by topics, but many of them were very similar to their experiences, brought active discussion on the issues. Some could not arouse sympathy because of cultural differences. The teachers recognized the dilemma episodes provided thoughtful reflection on their own teaching, opportunity of sharing experience and knowledge with peers, overall view for science education. Most of them gave positive opinion on the use of dilemma episodes in teacher education. Upon these teachers' responses and exploratory research experiences, some practical suggestion were made for science teacher educators.

Screeening of Natural Plant Resources with Acetylcholine esterase inhibitory activity and Effect on Scopolamine-induced Memory Impairment (천연식물자원으로부터 Acetylcholine esterase 저해 활성 탐색 및 인지기능에 미치는 영향)

  • Choi, Jang Won;Won, Mu-Ho;Joo, Han-Seung
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.213-226
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    • 2011
  • This study was performed to investigate the effect of essential oils and ethanolic extracts of approximately 650 plant species on acetylcholine esterase (AChE) enzyme activity using Ellman's colorimetric method in 96-well microplates. The results showed that the ethanolic extracts from twig of Sophora subprostrata, twig of Phellodendron amurense, seed of Corylopsis coreana, and essential oil (EO) from Citrus paradisi, Cupressus sempervirens, Ocimum basilicum, Pinus sylvestris and Rosmarinus officinalis inhibited more than 80% of AChE activity. Among these, EO from Pinus sylvestris, C. sempervirens and C paradisi exhibited higher values of AChE inhibitory activity, which were 75, 84 and 99% at a concentration of 50 ug/ml, respectively. Finally, EO from C paradisi (grapefruit, GEO) showed the highest inhibitory activity towards AChE, which showed 91% of inhibition at a concentration of 20 ug/ml. We also examined the anti-dementia effects of GEO in mouse by passive avoidance test and Morris water maze test. The model mouse (male, ICR) of dementia (negative control) was induced by administration of scopolamine (1 mg/kg body weight). The latency time of sample group administrated with GEO (100 mg/kg, p.o.) increased significantly as compared with negative control on passive avoidance test. There were significant recovery from the scopolamine-induced deficits on learning and memory in water maze test through daily administrations with GEO (100 mg/kg, p.o.). From these results, we conclude that GEO treatment might enhance the cognitive function, suggesting that the EO of C. paradis may be a potential candidate for improvement of perceptive ability and dementia.

An Evaluation of Usability and Interface Design of Internet Shopping Mall (인터넷 쇼핑몰의 사용성 평가 및 인터페이스 설계)

  • 곽효연;신현숙
    • Journal of the Korea Society of Computer and Information
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    • v.6 no.2
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    • pp.157-162
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    • 2001
  • Internet shopping mall is done commercial trade in particular web sites, and zoomed with an important means of trade for the next generation. When users visit shopping mall, the best objective is determined to users by of offering satisfaction and pleasant experience. Therefore. this paper was selected a menu list as an evaluation factor to emphasize a usage convenience of internet shopping mall, and executed a performance and a subjective satisfaction evaluations on a screen design and a structure design. The results of all the performance and the subjective evaluations for the screen design were shown the shortest search times on left type of the main-menu and vertical type of the medium-menu. In the structure design, it was shown that users were the highest satisfaction in type that search product and shopping cart procedure were firstly processed, and the procedure of a shopping membership and paying a bill were secondly processed at the same screen with regard to a comprehensibility and convenience of purchase procedure. It was shown that the user prefered the shopping mall web site to analogy with the real purchase procedure

Analysis of Antifungal Proteins in Streptomyces sp. SAR01 (Streptomyces sp. SAR01 균주에서의 항진균 관련 단백질 분석)

  • 이영근;김재성;조규성;장병일;추철형
    • Korean Journal of Environmental Biology
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    • v.20 no.3
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    • pp.237-244
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    • 2002
  • To analyze proteins related to antifungal activity, SAR01 strain was isolated from seaweed and identified as Streptomyces sp. from the result of FAME (fatty acid methyl ester) analysis. The isolated strain had antifungal activities against T species of plant pathogenic fungi. Antifungal activity deficient mutant (SAR 535) of Streptomyces sp. SAR01 was induced by gamma radiation $(^{60}Co,\;5kGy)$. By 2 D electrophoresis analysis, 6 protein spots were found in wild strain (SAR01) but these spots disappeared in mutant strain (SAR535). Among them, 5 proteins showed similarities to heat shock protein 70(HSP70), Fe-containing superoxide dismutase II (Fe- SODII), ribosome recycling factor (RRF), 10 kDa chnperonin (GroES) and inorganic pyrophosphatase (PPAse), respectively. It suggested that the above 6 proteins could be closely related to the antifungal activity of Streptomyces sp. SAR01.

Identification of Genes Expressed during Conidial Germination of the Pepper Anthracnose Pathogen, Colletotrichum acutatum (고추 탄저병균의 포자 발아 단계 발현 유전자 동정)

  • Kim, Jeong-Hwan;Lee, Jong-Hwan;Choi, Woobong
    • Journal of Life Science
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    • v.23 no.1
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    • pp.8-14
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    • 2013
  • Genes expressed during conidial germination of the pepper anthracnose fungus Colletotrichum acutatum were identified by sequencing the 5' end of unidirectional cDNA clones prepared from the conidial germination stage. A total of 983 expressed sequence tags (ESTs) corresponding to 464 genes, 197 contigs and 267 singletons, were generated. The deduced protein sequences from half of the 464 genes showed significant matches (e value less than 10-5) to proteins in public databases. The genes with known homologs were assigned to known functional categories. The most abundantly expressed genes belonged to those encoding the elongation factor, histone protein, ATP synthease, 14-3-3 protein, and clock controlled protein. A number of genes encoding proteins such as the GTP-binding protein, MAP kinase, transaldolase, and ABC transporter were detected. These genes are thought to be involved in the development of fungal cells. A putative pathogenicity function could be assigned for the genes of ATP citrate lyase, CAP20 and manganese-superoxide dismutase.

Immune Gene Discovery by Expressed Sequence Tags Generated from Olive Flounder (Paralichthys olivaceus) Kidney (넙치 (Paralichthys olivaceus) 신장에서 생성된 ESTs (Expressed Sequence Tags)로부터 면역관련 유전자의 탐색)

  • Lee, Jeong-Ho;Kim, Young-Ok;Kim, Jong-Hyun;Noh, Jae Koo;Kim, Hyun Chul;Kim, Kyung-Kil;Kim, Kyu-Won
    • Korean Journal of Ichthyology
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    • v.18 no.4
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    • pp.283-292
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    • 2006
  • Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the kidney mRNA of olive flounder (Paralichthys olivaceus). In the survey of 390 ESTs chosen from the kidney cDNA library, 250 ESTs showed significant homology to previously described genes while 140 ESTs were unidentified or novel. Comparative analysis of the 250 identified ESTs showed that 14 (5.6%) clones were representing 11 unique genes identified as homologous to the previously reported olive flounder ESTs, 198 (79.2%) clones representing 160 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 38 (15.2%) clones representing 37 genes of known sequences with unknown functions. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immunerelated genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

A Comparative Study on Consumer Groups based on Consumers' Attitude and Purchase Intention of Luxury and Masstige Brands, and Counterfeits (명품과 매스티지 브랜드, 복제품에 대한 태도 및 구매의도에 따른 소비자집단 비교연구)

  • Bang, Junghae;Kim, Min Sun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.303-309
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    • 2021
  • This study examined how consumers in their 20s and 30s perceive luxury goods, masstige brands, and counterfeits, which have high social value, and whether the propensity for conspicuous consumption and conformity can explain them. Consumers were classified based on the similarity of attitudes toward these brands and purchase intentions. The groups were compared on the sub-dimensions of conspicuous consumption and conformity. Clustering analysis identified four groups, and the MANOVA result confirmed the differences among the groups. Personality (F=4.282, p=.006) and brand orientation (F=23.178, p=.000) were positively related to luxury and masstige brands. Fashion orientation (F=8.376, p=.000) was high for both groups, which likes luxury and masstige brands very much, and which likes counterfeits. High-price orientation did not make any significant difference among the groups. Conformity (F=3.537, p=.015) was high for all the groups, except for the group that liked luxury and masstige brands and did not like counterfeits. This study comprehensively examined the attitudes and purchase intentions of luxury and masstige brands and their counterfeits and can be the groundwork for further research on brand categorization.

A Heuristic Search Algorithm for Vehicle Routing Problem with Mixed Delivery and Pick-up (배달과 수거의 혼합적재가 허용되는 차량경로문제의 발견적 탐색해법)

  • Chung, Eun-Yong;Park, Yang-Byung
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2004.05a
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    • pp.315-318
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    • 2004
  • 지금까지 대부분의 물류시스템은 생산자에서 최종 소비자에 이르는 생산물품의 수${\cdot}$배송에만 집중되어 왔으나, 최근 산업계에서는 기존의 수송시스템에 역방향 물류시스템을 통합하려는 시도가 이루어지고 있다. 본 논문에서는 배달화물과 수거화물이 혼합 적재되는 차량경로문제를 제안하고 이를 해결하기 위한 방안으로 유전알고리듬을 적용한 해법을 제시한다. 배달과 수거의 혼합적재를 허용함에 따라 고객지점에서는 적재된 혼합화물을 재배치하는 문제가 발생할 수 있다. 이 때 소요되는 적재화물의 재배치시간은 직관적인 수식으로 표현하였다. 유전알고리듬 개발과정에서 여러 가지 교차 연산자와 돌연변이 연산자들을 새롭게 고안하였다. 제안된 유전알고리듬 해법의 성능평가를 위하여 유사한 차량경로문제를 다루는 최신 알고리듬과 비교 계산실험을 수행하였다. 다양한 문제를 구성하여 실험한 결과, 제안된 유전알고리듬해법의 효과성이 입증되었다.

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Efficient Integrity Checking using Hashed B-Tree Index (Hashed B-트리 인덱스를 이용한 효율적인 무결성 검사)

  • Park, Sun-Seob;Jeong, Jae-Mok;Kim, Hyoung-Joo
    • Journal of KIISE:Databases
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    • v.27 no.2
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    • pp.216-226
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    • 2000
  • This paper suggests a new access path, hashed B-tree which is an efficient access method for integrity checking. Hashed B-tree is based on the observation that most query patterns in enforcing integrity constraints are point queries. Hashed B-tree compresses the key by hashing procedure, which reduces the height of tree and results in fast node search. This method has the advantages such as it can be implemented easily and use the B-tree concurrency control and recovery algorithm with minor modifications.

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