Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Immune Gene Discovery by Expressed Sequence Tags Generated from Olive Flounder (Paralichthys olivaceus) Kidney

넙치 (Paralichthys olivaceus) 신장에서 생성된 ESTs (Expressed Sequence Tags)로부터 면역관련 유전자의 탐색

  • Lee, Jeong-Ho (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Young-Ok (Biotechnology Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Jong-Hyun (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Noh, Jae Koo (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Hyun Chul (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Kyung-Kil (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Kyu-Won (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University)
  • 이정호 (국립수산과학원 어류육종연구센터) ;
  • 김영옥 (국립수산과학원 생명공학연구단) ;
  • 김종현 (국립수산과학원 어류육종연구센터) ;
  • 노재구 (국립수산과학원 어류육종연구센터) ;
  • 김현철 (국립수산과학원 어류육종연구센터) ;
  • 김경길 (국립수산과학원 어류육종연구센터) ;
  • 김규원 (서울대학교 약학대학)
  • Received : 2006.11.20
  • Accepted : 2006.12.18
  • Published : 2006.12.31
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Abstract

Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the kidney mRNA of olive flounder (Paralichthys olivaceus). In the survey of 390 ESTs chosen from the kidney cDNA library, 250 ESTs showed significant homology to previously described genes while 140 ESTs were unidentified or novel. Comparative analysis of the 250 identified ESTs showed that 14 (5.6%) clones were representing 11 unique genes identified as homologous to the previously reported olive flounder ESTs, 198 (79.2%) clones representing 160 unique genes were identified as orthologs of known genes from other organisms, and orthologs were established for 38 (15.2%) clones representing 37 genes of known sequences with unknown functions. We also identified several kinds of immune associated proteins, indicating EST as a powerful method for identifying immunerelated genes of fish as well as identifying novel genes. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

넙치 (Paralichthys olivaceus) 신장에서 추출한 mRNA로부터 cDNA library를 제작하고 이를 이용하여 EST (Expressed sequence tag) 분석을 하였다. 넙치의 신장 cDNA library에서 무작위로 선별한 390개의 EST를 조사한 결과, 250개의 EST는 이미 밝혀진 유전자와 유사성이 있는 것으로 나타났으며, 140개의 EST는 새로운 유전자로 밝혀졌다. 유전자의 기능이 밝혀진 250개의 EST 중 14 (5.6%)개의 EST는 이미 알려진 넙치 EST와 상동성이 있는 유전자로 확인되었고, 198 (79.2%)개의 EST는 다른 생물에서 알려진 유전자와 상동성이 있는 것으로 나타났다. 그러나 38 (15.2%)개의 EST는 전혀 기능이 알려지지 않은 새로운 유전자로 밝혀졌다. EST 분석은 새로운 유전자 뿐만 아니라 기능적으로 중요한 유전자를 탐색하는데도 아주 강력한 연구 방법이다. 이에 따라 본 연구에서는 넙치 신장 EST 분석을 통해 C-, L-type lectin과 MHC class II-invariant chain like proteins (li) 같은 면역기능과 관련이 있는 여러 개의 유전자를 확인하였고, 이들 유전자들은 질병감염 후 유전자 발현에서 나타나는 차이를 분석하는데 이용되는 cDNA microarray 연구에 유용하게 사용될 것으로 보인다.

Keywords

Acknowledgement

Supported by : National Fisheries Research and Development Institute

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