• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.265-268
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• 1996

According to the research of the cultivation result of Gardenia for 5 years to investigate its cultivation conditions and problems, the development of the variety with early mature and high yielding is urgent and that of rooted cutting cultivation method by the experiment of rooted cutting, bed soil, growth regulator and cutting period are pressing. The development of cultivation method with planting year, planting density, supplementary period and amounts of supplementary of Gardenia planted on growing field are required and for the disease and insect pest control, the research of the cultivation method with disease and insect pest resistance is ne­eded by auxotrophic fertilizing and organic agricultural method, not by agriltural chemicals.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.7-11
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• 1995

Protoplasts were isolated from hypocotyl tissues of 5-day-old Brassica oleracea var capitata Green Challenger seedlings. Several media were used for protoplast culture and shoot regeneration. The shoot-regeneration rapacity of protoplast derived callus depended on the initial culture medium. Protoplasts were cultured in liquid medium (B5 medium supplemented with CaCl2, 2H2O 600mg/L, g1ucose 20g/L, D-mannito1 70g/L, NAA lmg/L, BA lmg/L, 2.4-D 0.25 mg/L)at 27$^{\circ}C$ under the dark After 5 to 10 days, cultlues were diluted with medium with a reduced osmotic stabilizer and then transferred to illuminated conditions. The culture medium was changed with the fresh medium at 7- to 10-day-intervals until the formation of microcallus. Hypocotyl protoplast-derived callus proliferated when transferred to MS medium supplemented with NAA lmg/L, BA 1mg/L and GA$_3$ 0.02mg/L. Upon transfer to MS basal medium without growth regulators, roots were produced. In an attempt to increase the regeneration frequency, 10g/L polyvinylpyrrolidone was added to the regeneration medium, but the shoot regeneration was mot improved. The regenerated whole plants were acclimated in a sterized soilless mixture(vermiculite 2;perlite 2;peat moss1) in a culture room.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.103-106
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• 1997

In order to establish a micropropagation system of Naricissus, the ability of bulblet regeneration among propagation materials was compared, and the adequate growth regulators and concentrations for each cultivar were investigated. The inorganic components were also assayed in the parts of propagation materials. In propagation materials, scape with based plates showed hightest rate of bulblet formation and rapid growth of formed bulblets in vitro, comparing to other parts of it. In comparing of varieties, 'Dutch Master' and 'Golden Harvest' showed a high ability for bulblet regeneration. The ability of bulblet regeneration was most favorable in the medium, supplemented with 5.0 mg/L BA and 2.5 mg/L NAA in 'Dutch Master', and 5.0 mg/L BA and 1.0mg/L NAA in 'Golden Harvest', respectively. In inorganic component analysis of propagation materials, the White part of scape contained 1.18 mg/L$P_2O_5$, 2.57 me Ca, 0.94 me Mg and 3.20 mg/L total N. It showed higher levels in concentration of inorganic components as compared to those of the other part of scape. In addition, leaves and yellow part of scape contained significantly high levels of Ca and Mg while scales bulb showed considerably low levels in all inorganic compounds.

• The Korean Journal of Pesticide Science
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• v.8 no.2
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• pp.129-136
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• 2004

In order to determine the amounts of impurities and to identify the chemical structures of the impurities in the technical product of the plant growth regulator Atonic, the extracts of diethyl ether and dichloromethane were analyzed with GC-FID and GC-MSD. resulting in detection of five impurities and identification of their chemical structures. The amount of the active ingredient atonic in the technical product was about 84% and those of the impurities ranged from 0.24 to 10.74%. The identified impurities in this technical product are 2-methoxyphenol (guaiacol, m/z 124), 2-chloro-6-methoxyphenol and/or 4-chloro-6-methoxyphenol (m/z 158), 1,2-dimethoxy-4-nitrobenzene (m/z 183), and 2,6-bis(1,1-dimethylethyl)-4-methylphenol (m/z 220), suggesting that they are not hazardous impurities.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.240-245
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• 1995

This study was conducted to find out environment conditions in order to increase of growth and yield of tuberlets in pinellia ternata (Thunb.) Breit. The optimal temperature conditions for the growth of tuberlets were good rather temperature fluctuations than constant one and the optimal photoperiod was a 6hr-photoperiod. The growth of tuberlets to the kinds and concentration of growth regulators was good for 0. lppm 2.4- D, 10ppm kinetin and 50ppm gibberellin treatment. In colchicine soaking treatment, the growth of tuberlets was bad, but bulblets formation was good for 0. 05% colchicine treatment for 48hr. -soaking. In colchicine dropping treatment, dry tuber yield was good for 0. 1% colchicine treatment with once dropping for one day.

• Korean Journal of Medicinal Crop Science
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• v.20 no.5
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• pp.353-358
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• 2012

This study was conducted to enhance the propagation of Acanthopanax divaricatus and Acanthopanax koreanum through different cutting practices. For propagation by cuttings, scions were taken on September 1. This already has hard skin and showed much higher values in terms of root length, root number, rooting ratio and root weight than those taken on June 30 and August 1. Regardless of cutting date, 50% shading resulted to the highest root length, root number, root ratio and root weight. Meanwhile, 95% shading significantly reduced these parameters compared with no shading. These results suggest that over-shading may inhibit root growth. Two growth regulators, IBA (concentration of 1,000, 2,000, 3,000 ppm) and Rootone-F (0.4%) were also tested of its effect to the cuttings. Rootone-F was found to be more effective than IBA. Cutting treated with Rootone-F had slightly higher root length and root number. A. koreanum which grows well in hot climatic condition showed better rooting ability than A. divaricatus.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.21-26
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• 1999

These experiments were attempted to introduce rolC gene in the Petunia hybrida cv. Titan white by Agrobacterium mediated. The maximum frequency of shoot regeneration was obtained by 60% on MS medium containing 1.0 mg/L BA, 0.1 mg/L NAA, 200 mg/L kanamycin, 500 mg/L carbenicillin, 30 g/L sucrose, and 8 g/L agar. Kanamycin-resistant calli were selected from petunia leaf discs by cocultivation with Agrobacterium suspension cultures on MS medium. The addition of AgNO$_3$ and KMnO$_4$ in the medium increased the shoot regeneration by 31.3% from leaf disc as compared with non-treated leaf disc. Among clones exhibiting kanamycin resistance, only 3 clones were confirmed by southern hybridization analysis.

• Asian Journal of Turfgrass Science
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• v.19 no.2
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• pp.125-130
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• 2005

The effects of various pretreatment in Zoysia japonica are summarized as follows: Seeds that were treated for 7 days in $4^{circ}$C showed a little higher germination rates than those that were treated 3 days in $4^{circ}$C and for 1 day, 3 days in $-20^{circ}$C. Germination rates of the seeds that were soaked in NaOC1 showed higher germination rates than control group. BA 0.1 mg$\cdot$$L^{-1}$ treatment showed a little higher germination than GA3 0.1 mg$\cdot$$L^{-1}$, NAA 0.1 mg$\cdot$$L^{-1}$. Seed germination rates increased around $20^{circ}$C when the samples treated by BA and scarification. Leaf morphological didn't different between control and pretreatment groups.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.139-145
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• 1973

Effects of Choline Chloride on growth and yields of sweet-potato cultivated with the single crop and after-barley crop were summarized as follows. 1. The greatest effect was obtained when the sprout-bases of sweet potato were soaked in the solution of Choline Chloride for 24 hours, while the optimum concentration of Choline Chloride was 32.3ppm in case of single crop and 31.1ppm in after barley crop respectively. 2. Choline Chloride restrained the growth of stem; the length shortened and the dry weight decreased. 3. The number of tubers and yields were increased by treating choline chloride. It may be thought that the translocations of assimilation substance from leaves and stem to tubers, was stimulated by treating choline chloride. The tendancy of higher yielding was shown in the early harvesting than in the usual harvesting 4. Sugar and starch content were higher, crude fiber and crude protein content were lower as compared with the control.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.53-58
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• 2003

This study was performed to investigate the effects of various media compositions in regeneration of Lilium lancifolium. The adventitious bud initiation from microscale was the best on MS medium supplemented with BAP 1.0 mg/L and NAA 0.1 mg/L after 4 weeks of culture. However, from bulbscales, adventitious bud initiation was the best in dark condition on MS medium supplemented with BAP 0.5 mg/L and NAA 0.1 mg/L. On the other hand, callus induction was found to be the best from the microscales incubated in complete dark condition for 8 weeks on MS medium supplemented with 2,4-D 1.0 mg/L and BAP 0.1 mg/L. The highest plantlet regeneration from callus was obtained after incubation in the light condition for 8 weeks on MS medium supplemented with NAA 0.5 mg/L and BAP 0.1 mg/L. Rooting of shoots was obtained easily on MS medium and the plantlets were transferred to soil pots after 8 weeks. The chromosome analysis of the root tip cells was revealed that the callus-derived plantlets had normal chromosome number, 2n=24. No variation was observed in the morphology of the plantlets.