• Korean journal of applied entomology
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• v.34 no.4
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• pp.368-372
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• 1995

To develop a microbial pesticide for the control of pine gall midges. Thecodiplosis japonensis, entomopathogenc fungi were isolated from 233 soil samples in the damaged region of Thecodiplosis japonenesis, and identified with Beauveria spp. 29 strains and Paecilomyces spp. 2 strains. The morphology of entomopathogenic fungi was observed by scanning electron miroscope. In addition, the toxicity of entomopathogenic fungi was observed by scanning electron microscope. In addition, the toxicity of entomopathogenic fungi isolated from soil samples was determined by bioassay against Thecodiplosis japonensis larvae. The result showed that toxicity of relatively pathogenic strains, Beauveria spp. SFB-168-2 was 82.9%, suggesting that Beauveria spp. SFB-168-2 is effective entomopathogenic fungi for the control of pine gall midges.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.1
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• pp.170-179
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• 1996

누룩은 생소맥을 조분쇄하여 물로 혼합시켜 자연적으로 공지중의 여러 종류의 미생물을 배양시켜 만들었다. 그러므로 누룩에서는 많은 종류의 사상균, 호모와 세균이 증식하였다. 누룩의 당화력은 원료 생소맥의 당화력과 대부분의 사상균의 당화력과 알붕의 세균성 당화력에 기인하여, 발효력은 누룩효모와 극소수의 사상균애 의하여 이루어지고 있다. 1945년 이전까지 누룩으로부터 12속 59종의 사상균이, 8속 29종의 효모와 4속 16종의 세균이 분리 되었다. 누룩사상균은 Aspergillus 속이 주종을 이루었고 Rhizopus속, Absidia속, Nucor속의 순으로 분포하고 있었고, Aspergillus속과 Rhizopus속은 중요한 누룩 당화균으로 작용하였다. 누룩의 대표적 효모는 Saccharomyces cerevisiae 이며 , 이균이 알코올 발효에 가장 큰 역할을 담당하며, Saccharomyces cerevisiae도 전통 민속주의 탁주와 약주의 발효역에 깊이관여한다고 사료된다. 누룩 중에 증식하는 세균은 양조학상 특별한 역할을 담당하지않지만 Bacillus 속 과 젖산세균이 많이 분리되었으며, 젖산세균은 담금초기에 pH의 안정화에 기여할 수 있다고 추측할 수 있다.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.97.2-97
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• 1978

숙성한 퇴비에서 16종의 사상균을 분리하고 이들의 xylanase 및 cellulase의 활성을 측정하였다. 이 결과 효소활성이 강한 6 균주를 선별하고 이들의 형태학적 특성을 속까지 동정하고 선별된 균주가 생산하는 xylanase와 cellulase의 성질을 비교 검토하였다. 선별된 균주의 배양기질에 따른 효소생산량 산량을 비교하기 위해 cellulose와 xylan으로 배양한 후 이들을 분해하는 효소활성의 비 즉 xylanase/cellulase 비를 계산하고 이들 효소의 일반 성질을 검토하였다. 차후 연구에서 이들 효소를 분리, 정제하기 위해 acetone에 의한 침전성을 아울러 실험하였다.

• Korean Journal of Environmental Agriculture
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• v.14 no.3
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• pp.345-350
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• 1995

Another two different small composters with the double layer walls(Type 3 and Type 4) were made for dwelling house. One was insulated(Type 4) but the other uninsulated(Type 3). The change in microbial flora has been investigated through laboratory composting using these composters. The results were summarized as follows. 1. While the number of mesophilic bacteria decreased, that of thermophilic bacteria increased in winter. But thermophillic bacteria and mesophillic bacteria showed a tendency to increased in winter. But thermophilic bacteria and mesophillic bacteria showed a tendency to increase and decreas simultaneously in spring and summer at the early stage of composting. 2. The number of mesophilic actinomycetes ans thermophilic actinomycetes were decreased after I week in winter, while thermophilic actinomycetes rapidly increased in spring and mildly increased in summer. 3. The number of mesophilic fungi and thermophilic fungi had a tendency to increase and decrease simultaneously at an early stage of composting except after I week in winter. 4. Mesophilic bacteria, actinomycetes and fungi showed no difference in the number of microbes, but the number of fungi in spring was smaller than in other sensons. 5. At the late stage of composting process, the number of mesophilic fungi was decreased in winter and summer but increased in spring.

• Journal of the korean veterinary medical association
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• v.18 no.4
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• pp.64-65
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• 1982

• Environmental engineer
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• s.52
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• pp.18-24
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• 1990

• Environmental engineer
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• s.51
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• pp.26-31
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• 1990

• Applied Biological Chemistry
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• v.13 no.3
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• pp.231-235
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• 1970

1. Purification of amylase system produced from Humicola sp. by a submerged culture eras carried out. 2. By DEAE-Cellulose column chromatography amylase system was separated into two fractions eluted at 0.05M and 0.5M phosphate buffer solution of pH 6.0. 3. The saccharogenic amylase was mostly composed of. the fraction of 0.05M phosphate buffer solution of pH 6.0 while the dextrinogenic amylase was perseted in fraction of 0.5M phosphate buffer solution of pH 6.0 4. It was found that the optimum pH of this saccharogenic amylase was within the range of from 4.5 to 5.5, stable pH was within the range of from 4.0 to 9.0 and optimum temperature was $60-65^{\circ}C$. This amylase was stable at $70^{\circ}C$ for ten minutes but completely inactivated $80^{\circ}C$ above.

• Journal of Sericultural and Entomological Science
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• v.37 no.2
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• pp.167-171
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• 1995

To develope a microbial pesticide for the control of mulberry longicorn beetle, Apriona germari, Beauveria spp. were isolated from the infected Apriona germari larvae. The morphology of Beauveria spp. was observed by phase contrast and scanning electron microscope. In addition, the Beauveria spp. isolated from Apriona germari were identified by the random amplification of polymorphic DNA using polymerase chain reaction. The results showed that the Beauveria spp., SFB-1A and SFB-3A, isolated from Apriona germari were identified with B. bassiana and B. brongniartii, respectively, suggesting that the random amplification of polymorphic DNA is effective for the identification of Beauveria spp.

• Applied Biological Chemistry
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• v.15 no.3
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• pp.207-211
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• 1972

In order to investigate the action of thermophilic fungi on the xylan and its related substances, xylanase and laminaranase were examined using Myriococcum albomyces. In the extract from bran culture of Myriococcum albomyces, xylanase and laminaranase activity were recognized. 1) The optimum pH for xylanase and laminaranase activity were found to be pH 5.0 and pH 6.0. 2) The optimum temperature for xylanase and laminaranase activity were found to be $55^{\cicr}C$. 3) Thermal stability of xylanase for 55 minutes at $65^{\cicr}C$ and laminaranase for 60 minutes at $65^{\cicr}C$ did not influence their stability.