• Title/Summary/Keyword: 변형시기

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Construction and Analysis of Binary Vectors for Co-Overexpression, Tissue- or Development-Specific Expression and Stress-Inducible Expression in Plant (식물에서 표적 유전자의 동시 과발현, 조직/발달 특이적 발현 및 스트레스 유도성 발현을 위한 binary 벡터의 제작과 분석)

  • Lee, Young-Mi;Park, Hee-Yeon;Woo, Dong-Hyuk;Seok, Hye-Yeon;Lee, Sun-Young;Moon, Yong-Hwan
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1314-1323
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    • 2010
  • In this study, we constructed various kinds of binary vectors with the pPZP backbone for co-overexpression, tissue- or development-specific expression and stress-inducible expression, and validated them for ectopic expression of target genes. Using a modified CaMV 35S promoter, a binary vector was generated for co-overexpression of two different genes and was confirmed to be efficient for overexpressing two different target genes at the same time and place. Binary vectors containing At2S3, KNAT1 or LFY promoters were constructed for tissue-specific or development-specific gene expression, and the binary vectors were suited for embryo/young seedling stage-, shoot apical meristem- or leaf primordia-specific expressions. Furthermore, the binary vectors containing RD29A or AtNCED3 promoters were validated as suitable vectors for gene expression induced by abiotic stresses such as high salt, ABA, MV and low temperature. Taken together, the binary vectors constructed in this study would be very useful for analyzing the biological functions of target genes and molecular mechanisms through ectopic expression.

A Study on the Mushrooms Cultivation Using Ozon (오존을 이용한 버섯재배에 관한 연구(1))

  • Chang, Hyun-You;Byun, Jae-Myun;Nho, Moon-Gee;Park, Soon-Ae
    • Journal of Agricultural Extension & Community Development
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    • v.7 no.1
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    • pp.181-191
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    • 2000
  • This experiment was conducted to find out the effects of mushroom cultivation on ozone susceptibility. The mushroom media in cultivation became softening well without bad Knell when it was soaked for 120 hours. The mushroom media softening on cultivating of oyster mushroom was good and fast when the concentration of gas condition ozone is high. The concentration of gas condition ozone must control at less than 0.02 ppm at the innoculation room, 0.03 ppm at the incubating room, must not use at the sprouting mom, less than 0.02 ppm at the growing room respectively. Also at the growing room, it removes the smell of mushroom, so has a protective effects of coming into mushroom fees and disease. When the concentration of 0.02 ppm liquid condition ozone was sprinkled, it was very useful in disease protection If it was input, the storage period of mushroom fruiting body was prolonged.

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Studies on the Life History of Three Spotted Plusia (Chrysoideixis agnata S.) in the laboratory (콩은무늬밤나방의 생활사에 관한 연구)

  • Hwang C.Y.;Uhm K.B.;Choi K.M.;Hyun J.S.
    • Korean journal of applied entomology
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    • v.19 no.2 s.43
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    • pp.85-89
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    • 1980
  • This experiment was carried out to investigate the life history of the Three spotted plusia (Chrysodeixis agnata Staudinger) in the laboratory in 1976. (1) Female deposited $1202{\pm}150.6$ eggs during the oviposition period which was $13.3{\pm}1.2$ days. (2) Especially, larvae were distinguished with 5-instar type and 6-instar type. The egg period was 2 days and adult longevity was $21.29{\pm}3.15$ days for female and $19.14{\pm}2.47$ days for male. Larval period and pupal period were $15.36{\pm}1.59,\;7.82{\pm}1.01$ days for the 5-instar type and $16.92{\pm}1.08,\;8.23{\pm}1.09$ days for the 6-instar type. (3) Width of head capsule of the 5-instar type was $2.77\~2189mm$ and this of the 6-instar type was $2.78\~22.38mm$. (4) A highly significant correlation was recognized between the log-width of head capsule and the number of instar.

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Morphotectectics of the Shackleton Fracture Zone around the Antarctic-Scotia plate boundary off the northern Antarctic Peninsula (남극반도 북부 남극-스코시아 판경계부에서의 셰클턴 파쇄대의 지형지체구조)

  • Jin, Young-Keun;Kim, Yea-Dong;Nam, Sang-Heon;Kim, Kyu-Joong
    • Journal of the Korean Geophysical Society
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    • v.3 no.3
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    • pp.141-152
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    • 2000
  • In the vicinity of the Antarctic-Scotia plate boundary off Elephant Island(EI), geophysical data(multichannel seismic and gravity data) reveal rapid structural variation of the Shackleton Fracture Zone(SFZ) along its strike. The SFZ ridge terminates in front of the Antarctic Peninsula margin, whereas the transform fault of the SFZ continues farther southeast near EI and the width of the SFZ broadens toward the southeast. Accordingly, the SFZ transform fault changes its morphology along its strike as (1) a graben structure along the high Shackleton ridge in Drake Passage, (2) a half-graben structure in oceanic crust just southeast of the Antarctic-Scotia plate boundary, and (3) splay faults deforming the margin of EI. Two phases of tectonic deformation are clearly observed along the transform fault. Major extensional deformation had formed a large-scale half-graben during roughly about $10{\sim}20$ Ma when Drake Passage had opened. And then, the Shackleton fault has been reactivated with reverse sense, which has been caused by recent convergence between Antarctic and Scotia plates due to westward movement of the Scotia plate since 6 Ma.

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A Numerical and Experimental Study on Structural Performance of Noncomposite and Composite Eco-Arch Structures subjected to Concentrated Loads (집중하중을 받는 비합성.합성 생태아치구조물의 성능평가를 위한 수치해석 및 모형실험 연구)

  • Kim, Yong-Hee;Park, Jong-Sup;Lee, Young-Ho;Oh, Min-Su
    • Journal of Korean Society of Steel Construction
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    • v.22 no.2
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    • pp.173-183
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    • 2010
  • In this study, noncomposite and composite eco-arch structures with I-beams and precast concrete(PC) decks were investigated. Four finite-element models(a steel-girder model, a steel-girder-and-several-PC-panels model, a three-steel-girder model, and a three-steel-girder-and-several-PC-panels model) using a general finite-element program, ABAQUS, were reviewed to predict the strength of the noncomposite and composite arch structures. Based on the results of the finite- element analysis, the behaviors of the four models were investigated, and deflection and strain gauges for the experimental specimen consisting of three steel girders and several PC panels were set up to obtain the ultimate strength. The ultimate strength of the specimen was estimated to be 1,961kN. The ultimate strength was much larger than the 1,380-kN load calculated using AASHTO LRFD Bridge Design Specifications(2007). The noncomposite and composite arch bridges were found to have enough strength for safety.

The Diagnosis and Treatment of Anterior Openbite Malocclusion (전치부 개방교합의 진단과 치료)

  • Chang, Young-Il;Moon, Seong-Cheol
    • The korean journal of orthodontics
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    • v.28 no.6 s.71
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    • pp.893-904
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    • 1998
  • There are varieties of severe malocclusions, which can be treated orthodontically, but with a great deal of effort. Anterior openbite, in particular, is one malocclusion thought to be more difficult to treat, and therefore, most of them have to be corrected by means of surgical intervention. To solve these problems, numerous studies pertinent to treatment modalities have been introduced with controversies on the effectiveness of treatment. Suggested treatment modalities for anterior openbite are based directly or indirectly on the neuromuscular and morphological features and on the etiologic and/or the environmental factors. Even though the vertical relationship of the face is increased due to the growth variation, the normal occlusal relationship can be achieved by the adequate dentoalveolar compensatory mechanism, but in the case of inadequate or negative dentoalveolar compensation, openbite is likely to be present. If the skeletal dysplasia is too severe to be solved by orthodontic treatment alone, combined treatment with surgery should be done to restore the function and the esthetics of the orofacial complex. In many cases, however, orthodontic alteration of the dentition pertinent to the given skeletal pattern with the proper diagnosis and treatment planning can bring satisfactory results. The treatment changes with the Multiloop Edgewise Archwire(MEAW) therapy occurred mainly in the dentoalveolar region and showed a considerable similarity to the natural dentoalveolar compensatory mechanism. In other words, the MEAW technique allows orthodontists to produce the natural dentoalveolar compensation orthodontically. Even if an openbite is corrected by the orthodontic dentoalveolar compensation suitable for the skeletal pattern, relapse may still occur by the persisting etiologic factors which originally prohibited the natural dentoalveolar compensation. The etiologic factors should be determined at the time of initial diagnosis and should be controlled during treatment and retention.

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Comparison of slim bristled and V-shaped orthodontic toothbrushes in patients with fixed orthodontic appliances (고정식 교정장치 장착환자에서 슬림모 칫솔과 V형 교정칫솔 간의 구강위생관리 효과 비교)

  • Choi, Jin-Hugh
    • The korean journal of orthodontics
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    • v.39 no.6
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    • pp.383-392
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    • 2009
  • Objective: The purpose of this study was to evaluate the efficacy of a slim bristled toothbrush compared with a V-shaped orthodontic toothbrush in patients with fixed orthodontic appliances. Methods: Thirty four orthodontic patients receiving edgewise treatment were randomly assigned to two groups, a slim bristled toothbrush (Nano silver slim care) and a V-shaped toothbrush (Oral-B). Plaque index, gingival index and bleeding index were recorded at the beginning of the study (baseline), 2 weeks, 4 weeks and 6 weeks after new toothbrushes were supplied and the results were analyzed statistically. Results: No statistically significant differences were found for plaque index, gingival index and bleeding index between toothbrush groups during the 6 weeks. Plaque and gingival indices were decreased at 2 weeks and 4 weeks but increased at 6 weeks in both toothbrush groups. Bleeding index in the V-shaped toothbrush group showed the lowest value at 2 weeks then increased at 4 weeks and 6 weeks but in the slim bristled toothbrush group decreased from 2 weeks through 6 weeks to under baseline levels. Patients in their twenties had significantly lower values than teenagers in the slim bristled toothbrush group (p < 0.013). Conclusions: The results would suggest that both of the toothbrushes are equally effective but the use of a slim bristled toothbrush may be of benefit in promoting gingival health for fixed orthodontic appliance patients in their twenties and over.

Effectiveness of Stretching Exercise in The Treatment of The Iliotibial Band Friction Syndrome (장경대 마찰 증후군의 치료에서 스트레칭 운동의 유용성)

  • Suh, Jeung-Tak;Hong, Seong-Hwak;Lee, Choon-Key
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.5 no.2
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    • pp.147-154
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    • 2006
  • Purpose: To analyze the effectiveness of the treatment with the stretching exercise in the patients with iliotibial band (ITB) friction syndrome. Materials and Methods: 22 patients with ITB friction syndrome were treated with the stretching exercise of the ITB. The criterion for determining the good result is more than 75% recovery according to the classification of success of treatment. We analyzed the differences of the duration of treatment at which the good result it achieved according to sincerity of the stretching exercise. The sincerity of the exercise is defined at performing more than 50% of the exercise that we prescribed. Results: Of 22 patients, there were good results in 19 cases (87%) within 8 weeks after the treatment. Except for 3 cases who did not achieved good results, the average duration of the treatment is 28.1 days. In patients with good results there was no statistical significance for the relationship between the duration of symptoms before diagnosis (average 25.6 days) and the duration of the treatment (average 28.1 days). In comparison with the group who performed the exercise sincerely and the group who did not, the average duration of the treatment if 23 days and 32.7 days respectively and there was significant difference between the two groups. Conclusion: The stretching exercise in ITB friction syndrome was helpful in reducing the duration of treatment.

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Structural Layers and History of Folding in the Western Part of the Baegunsan Syncline, Samcheog Coalfield, South Korea (백운산향사(白雲山向斜) 서단부(西端部)의 구조계층(構造階層)과 습곡작용(褶曲作用)의 과정(過程))

  • RHI, Jae-Young;PARK, Bong-Soon
    • Economic and Environmental Geology
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    • v.15 no.1
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    • pp.41-47
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    • 1982
  • Typical stratigraphic sequences of Paleozoic form the Baegunsan Syncline (equal to Hambaeg Syncline) and Taebaegsan Group is the basement in the southern part of Kangweon Province. Deformation pattern depends on their stratigraphic site and their internal properties. In the biotite schists and meta-sandstones of Pre-Cambrian sequences, flow folds and ptygmatic folds are developed, and high strain deformation is pervasive. Deformational patterns of Cambro-Ordovician are variable because of their various formations. Fracturing is pervasive in the Jangsan Quartzite and Daegi and Maggol Limestone. Welldeveloped slaty cleavage and angular folds of kink and chevron types are the prevailing structures of pelitic rocks. The most characteristic feature of limestone alternated with argillite is the "Compositional cleavage (Author's proposal)" known as the "Worm-eatern" structures. It was known that this structures have a sedimentary origin. But their preferred orienation of long axis of erosional lime holes, originated from tectonic deformation of folding. And this structures have the same character as the axial plane cleavages. Fracturing and long wave concentric folds are dominant characters of the Upper Paleozic sequences. In this area, two folds are superimposed. Field studying and analysis of structures show that the cross-folds of NS-direction are pervasive and were deformed by the EW-directional Baegunsan Syncline. The cross-folding is the major and penetrative deformation and prior to Baegunsan Syncline.

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Fine Structural Modification of Mouse Ovarian Tissue by Irradiation of 6 MeV LINAC Radiation (6 MeV LINAC 방사선 조사에 의한 생쥐 난소조직의 미세구조 변화)

  • Yoon, Chul-Ho;Moon, Myung-Jin
    • Applied Microscopy
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    • v.33 no.2
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    • pp.117-130
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    • 2003
  • This research investigates the fine structural as well as the morphological changes of the mouse ovarian tissue after irradiation of various dose rates of 6 MeV LINAC radiation. The normal structure of the ovarian tissue is consisted of various stages of follicles including primordial and growing follicles, and ovarian stromal connectives. When we observed the ovarian tissues irradiated with a dose rate of 200 cGy/min using light and electron microscopes, granular cells in growing follicles are in irregular shape unlike normal follicles. Small segments of cells scattered in follicular antrum among granular cells. We could observe neutrophils and macrophages around the segments, which means the cells already got in the process of decease owing to the effects radiation. With coincident to the increase of the dose rate of x-ray irradiation as 400 or 600 cGy/min, the mature follicles appeared as an irregular form and the granular cells surrounding oocyte also deformed comparing to their normal counterparts. The granulosa cells within mature follicle are already occurred necrotic change and apoptosis. The nuclei in some cells got so fragmented that the segments formed the shape of a horseshoe or scattered in small and condensed pieces. All the cells at a granular layer irradiated with a dose rate of 600 cGy/min show typical characteristics of apoptosis. The neutrophils involved in inflammatory reaction appear evidently in follicular antrum of growing follicles, and macrophage scattered with residual and apoptotic bodies.