• Journal of Agricultural Extension & Community Development
• /
• v.7 no.1
• /
• pp.181-191
• /
• 2000

This experiment was conducted to find out the effects of mushroom cultivation on ozone susceptibility. The mushroom media in cultivation became softening well without bad Knell when it was soaked for 120 hours. The mushroom media softening on cultivating of oyster mushroom was good and fast when the concentration of gas condition ozone is high. The concentration of gas condition ozone must control at less than 0.02 ppm at the innoculation room, 0.03 ppm at the incubating room, must not use at the sprouting mom, less than 0.02 ppm at the growing room respectively. Also at the growing room, it removes the smell of mushroom, so has a protective effects of coming into mushroom fees and disease. When the concentration of 0.02 ppm liquid condition ozone was sprinkled, it was very useful in disease protection If it was input, the storage period of mushroom fruiting body was prolonged.

• Journal of Mushroom
• /
• v.3 no.3
• /
• pp.115-118
• /
• 2005

This study was carried out to investigate the cultural charateristics of Fomitopsis pinicola in log woods. The optimal conditions for log cultivation as follows; 1) Light effect, natural light 2) $CO_2$, 0.2% below 3) Low temperature shock, $15^{\circ}C$ for 5 days 4) log-length, 100~120cm 5) It takes 52~65 days for primodia induction is possible to harvest fruiting bodies for 5~6 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.5 no.1
• /
• pp.8-12
• /
• 2003

The optimal log conditions of cultivation are as follows. 1) Log-length, 100~120cm 2) Hole-depth, 1~1.5cm 3) It takes 55~67 days for primodia induction is possible to harvest fruiting bodies for 6~7 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• The Korean Journal of Mycology
• /
• v.27 no.4 s.91
• /
• pp.289-292
• /
• 1999

We investigated the isolation method of mushroom infesting pests, Lycoriella mali, Coboldia fuscipes, Histiostoma sp. from mushroom-growing compost. Sugar solution of different densities (0, 10, 20, 30, 40, 50%) was tested to provide a means of seperating mushroom pests from the compost media. Thus, 40% sugar solution was suitable for isolation. The sieve size to entrap the pests was $30{\sim}140$ mesh; Lycoriella mali was trappped mainly $30{\sim}65$ mesh sieve, Coboldia fuscipes was caught mainly $30{\sim}100$ mesh sieve, Histiostoma sp. was trapped mainly $65{\sim}140$ mesh sieve. An isolation procedure was as follows; The infested compost was disintegrated in water and poured onto a set of 16, 30, 80, 140-mesh sieve. The material caught in the sieve is added in 40% sugar solution and then most compost particle were massed at the bottom while the supernatant contains mushroom pests. The upperlayer material was poured into a Seperatory funnel and the sediment at the bottom is drained off. The remaining material are washed off examination dish for study.

• Korean journal of applied entomology
• /
• v.37 no.2
• /
• pp.171-178
• /
• 1998

This study was conducted to investigate the effect of temperature on the development of amushroom-infesting sciarid fly, Bradysia sp. (Diptera: Sciaridae). Egg period was 12.1, 7.0, 4.4, 3.4, and3.2 days, larval period was 38.3, 26.5, 13.4, 13.2, 12.7 days and pupal period was 10.4, 7.1, 4.4, 3.3, 3.2days, and total development period from egg to adult emergence was 60.8,40.6, 22.2, 19.9, and 19.1 daysat 10, 15, 20,25, 28"C, respectively. Development threshold temperature (DT) and effective accumulativetemperatures (ET) were 3.8"C, 74.8DD in eggs, 1.2"C, 321.8DD in larva, and 3.1$^{\circ}$C, 76.5DD in pupa,respectively. The number of eggs laid per female was 107.9, 129.7, 131.8, 86.9, and 82.7 at respectivetemperatures. Preoviposition period was 6.6, 4.4, 2.2, 1.3, 1.8 days, oviposition period 1.5, 1.5, 1.1, 1.1,1.1 days, postoviposition period 2.0, 1.1, 0.9, 0.6, and 0.3 days at th'e temperature of 10, 15, 20, 25 and 28"C, respectively. The longevity of male and female at the temperature was 13.3, 7.8, 5.9,4.1, 3.4 days and10.4, 7.0, 4.2, 3.0, 3.3days, respectively. The optimum temperature for hatchability was estimated to the20$^{\circ}$C and adult emergence was highest at 20$^{\circ}$C. Pupation rate was 50.7, 68.4, 84.3, 86.5, 45.4% at 10, 15,20, 25, and 28"C, respectively. at 10, 15,20, 25, and 28"C, respectively.tively.

• The Korean Journal of Mycology
• /
• v.31 no.3
• /
• pp.134-140
• /
• 2003

Wild Mushrooms collected in Mt. Halla of Jeiu Island around altitude of $200{\sim}1000\;m$ from 1999 to 2000 were studied to investigate utilization as the natural breeding sites by wild drosophilids. Thirty-five mushroom species belonging to the 19 genera of 12 families from 2 orders were used as natural breeding sites by drosophilids. Adult flies emerged from 14 species of mushrooms belonging to the 11 genera of 8 families from 2 orders were identified. From the rest of 21 species of mushrooms, only larvae or/and pupae were reaped. Among 35 species of mushrooms, 34 species belonged to order Agaricales and only one species, Sarcodon scabrosum, belonged to order Aphyllophorales. In terms of composition of mushroom families, total 35 species belonging to 12 families were composed of 10 species of Russulaceae, 6 species of Amanitaceae, 4 species of each Tricholomataceae and Agaricaceae, 3 species of Boletaceae, 2 species of Strophariaceae and each one species of rest 6 families. Concerning the composition of the genus, 35 species belonging to 11 genera were composed of 8 species of Russula, 6 species of Amanita, 4 species of Agaricus, 2 species of Lactarius, and each one species of rest 7 genera. In terms of the mushroomsize preference of drosophilid flies for breeding sites, most flies preferred comparatively large mushrooms above 5 cm of pileus size in diameter, Fourteen species of drosophilids were identified as mushroom-breeding species. Among these, one and five species belonged to genera Mycodrosophila and Hirtodrosophila respectively, The genus Drosophila included the most abundant 8 species. Drosophila bizonata and Hirtodrosophila sexvittata bred on 6 species of mushrooms respectively, D. unispina bred on 3 species of mushrooms, rest species of Drosophila bred on one or two species of mushrooms. From the result of this study and other literatures, it could be presumed that much more mushroom species could serve as the natural breeding sites for greater number of drosophilid species than those examined in this study on Jeju Island.

• Journal of Mushroom
• /
• v.18 no.1
• /
• pp.72-78
• /
• 2020

During the monitoring of fungal pests in 2016 and 2017, Acrodontium crateriforme, Naganishia friedmannii, Pestalotiopsis trachicarpicola, Penicillium wollemiicola, and Trichoderma thailandicum were isolated from indoor air, mushroom flies (Phytosciara flavipes), and media materials in the cultivation houses of oak wood mushroom (Lentinula edodes) located in Seocheon, Jangheung, Buyeo, and Yeoju, Korea. These fungal species were identified based on their morphological characteristics after their growth on PDA and subsequent molecular analyses of the 26S rDNA, 28S rDNA, β-tubulin gene, and translation elongation factor 1-α gene using PCR amplification and nucleotide sequencing were performed. The results showed that these fungi were previously undocumented in Korea. This study reports descriptions of their taxonomical and known properties.

• Korean Journal of Plant Resources
• /
• v.12 no.3
• /
• pp.179-185
• /
• 1999

The study which was conducted to determine the effects of the polyethylene film on the culture of Pleurotus ostreatus is summarized in the following: 1) The fresh weight of Pleurotus ostreatus cultured by the box culture method mulching with black polyethylene film was 2,237g/box and, 2,028g/box by white polyethylene film mulching, and 1,695g/box at the conventional culture which was by 24.2% and 16.4% higher than that of the conventional culture.2) The fresh weight of P. ostreatus cultured by the shelf culture method mulching with the black polyethylene film was 17.4kg/m$^2$ at the white polyethylene film culture, 14.6kg/m$^2$ at the conventional culture which was by 16.2%, 7.5% higher than that of the conventional culture. 3) The best shape appearance in terms of the diameter in P. ostreatus was 5~30mm and the intervals were 10cm respectively. 4) The black polyethylene film mulching in P. ostreatus observed in good protection against Pseudomonas spp., Trichoderma, or mushroom flies. 5) The black polyethylene film mulching method for the culture of P. ostreatus was much better than that of the shelf-culture, box-culture or the sack culture in terms of total yield and quality. 6) The length of harvesting implement of P. ostreatus was suitable for 45~120cm in order to use on the harvest of the mulching.

• Journal of Korean Society of Forest Science
• /
• v.90 no.4
• /
• pp.543-547
• /
• 2001

An fungus gnat, Bradysia agrestis in the family Sciaridae was collected from Pinus spp. seedlings. This pest is a newly recorded insect from P. thunbergii, P. rigida, P. rigitaeda, and P. densiflora seedling. Brief morphological characters, damage symptom, host plants associated with the B. agrestis were recored. Significant variation in damaged of Pinus spp. seedling was attributed to differences between pine species(P. thunbergii : 15.4-20.0%, P. rigida : 23.1-48.8%, and P. rigitaeda : 13.8-67.5%, respectively) and sowing times(May : P. densiflora, 0%; P. rigida, 0%, July : P. densiflora, 20.0%; P. rigida, 49.4%, respectively).

• Journal of Life Science
• /
• v.10 no.1
• /
• pp.45-51
• /
• 2000

Ovarian development and yolk protein (YP) of mushroom fly, Coboldia fuscipes, were characterized. C. fuscipes has a pair of ovaries, composed of 130∼140 ovarioles, respectively. Ovarian development begins at 1 day of pupa, and growth of the ovaries continued to become a matured shape at 1 day after emergence. The YP of C. fuscipes identified by SDS-PAGE analysis revealed that the protein is composed of three subunits, designated YP1 (61 kDa), YPS(50 kDa), and YP3 (47 kDa). These three subunits of YP gradually decreased during embryogenesis. The YP was first detected in the 3 day-old pupal ovary and was continually detected up to 2 day-old adult, but not in the hemolymph. Furthermore, Western blot analysis of male and female adult hemolymph and ovary revealed that the antibodies against YP1, YP2, and YP3 reacted with three YP bands in ovary and egg extract, respectively. However, this reactivity was not observed in the male and female hemolymps. Therefore, it is assumed that the UP of C. fuscipes is synthesized in the ovary at 3 days after pupation.