In this study, we constructed and tested retrovirus vectors designed to express the human thrombopoietin gene under the control of the tetracycline-inducible promoters. To increase the hTPO gene expression at him-on state, WPRE sequence was also introduced into retrovirus vector at downstream region of either the hTPO gene or the sequence encoding reverse tetracycline-controlled transactivator (rtTA). Primary culture cells (PFF, porcine fetal fibroblast; CEF, chicken embryonic fibroblast) infected with the recombinant retrovirus were cultured in the medium supplemented with or without doxycycline for 48hr, and induction efficiency was measured by comparing the hTPO gene expression level using RT-PCR, western blot and ELISA. Higher hPTO expression and tighter expression control were observed from the vector in which the WPRE sequence was placed at downstream of the hTPO (in CEF) or rtTA(in PFF) gene. This resulting tetracycline inducible vector system may be helpful in solving serious physiological disturbance problems which have been a major obstacle in successful production of transgenic animals.
Journal of Korean Classical Literature and Education
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no.38
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pp.201-238
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2018
As a way of enhancing the intercultural ability needed for diverse cultural eras, this study focuses on the "narration" of the Italian education scholar Maddalena De Carlo in order to determine the "diverse values" created by the "symbolic representation" based on the folktales narrated by immigrants living in Korea. Through this, it specifically presents educational elements and contents that can raise relative sensitivity. The authors of this paper have connected, empathized, and communicated with people of various cultures in order to go beyond Carlo's discussion. The paper discusses the expansion of cultural sensitivity as an element of education through narrative topics using the folktales of immigrant narrators in Korea. It also recognizes the limitations of a desire for a homogeneous union within an intercultural society and thus formulates educational contents for creating a relationship with heterogeneous ideas through the elimination of communication barriers through heterogeneity and a consideration of the surface and the back. This is systemized in six steps. Step 1: Listening to oral folktales of immigrants, Step 2: Finding heterogeneous motifs imprinted in the immigrants' memories, Step 3: Understanding the meaning of the opposing qualities symbolized by heterogeneous motifs, Step 4: Creating narrative topics containing the key motifs, Step 5: Generating the value of symbolic representation as a narrative topic, and Step 6: Expanding the value of life into a cultural symbol. In Chapter 3, this study focuses on educational contents using immigrants' folktales by applying these six steps. The class contents include the recognition of the limitations of desire for a homogeneous union within an intercultural society and the consideration of how to create a relationship with heterogeneous ideas through the elimination of communication barriers through heterogeneity and consideration of the surface and the back. This paper then compares the Indonesian folktale, The Inverted Ship Mountain and the Mom's Mountain, with the world-famous Oedipus myth, to determine what the symbolic representation of these heterogeneous motifs is. In Step 6, when the symbolic system is culturally extended, the incestuous desire that appears in the "inverted ship" is interpreted as a fixation that was created when the character sought to unite with homogenous idea. The Cambodian folktale, The Girl and the Tiger, is a story that is reminiscent of the Korean folktale, The Old Man with a Lump. Through the motif in "Tiger," this paper generates a narrative topic that will enhance the students' intercultural abilities by culturally expanding their skills in how to relate with a heterogeneous being that is usually represented as an animal. The Vietnamese folktale, The Coconut Bowl, similar to the Korean folktale, GureongDeongDeong SinSeonBi, is a story that draws a variety of considerations about the surface and theback, and it shows readers how to build a relationship with a heterogeneous idea and how to develop and grow with such a relationship. Thus, if a narrative topic is generated and readers are able to empathize using an opposing feature formed by the core motif of the folktale, it becomes possible, through immigrant folklore, to construct a possibility of a new life through the formation of a relationship with an unfamiliar and heterogeneous culture.
The physicochemical characteristics and sensory properties of Deodeok wine, formed by leaching of Deodeok at room temperature for 180 days, were investigated over the following range of Deodeok levels: 10, 15 and 20% (all w/v). The higher the level of Deodeok, the greater were the final values of total sugars, reducing sugars, total polyphenols, and crude saponins. The Hunter's b-value (yellowness) of Deodeok wine varied markedly with Deodeok levels, and yellowness was highest in Deodeok wine containing 20% (w/v) Deodeok. Non-volatile compounds, that form the basis of the liquor tax law, were 0.64, 1.38 and 2.11% (all w/v), respectively, at day 160. Of these values, that of 2.11% (w/v), the level of non-volatile compounds in Deodeok wine containing 20% (w/v) Deodeok, was in accord with the liquor tax law (that requires this figure to be 2.0%). Sensory evaluation showed that Deodeok wine containing 20% (w/v) Deodeok was superior to the other wines tested.
Journal of Korean Home Economics Education Association
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v.35
no.2
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pp.145-161
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2023
The purpose of this study is to develop a content system for the home economics curriculum that integrates Education for Sustainable Development(ESD) and provides basic material for ESD implementation in schools. In view of this, the content elements of the revised home economics curriculum for 2022 were analyzed, and a content system for the home economics curriculum, linked to ESD, was proposed based on the implications drawn from the analysis. The results are as follows. First, the three components of competencies, namely knowledge, values, and skills, were organized equally as a whole. However, the association between the content elements and key competencies in sustainability was found to be insufficient. Consequently, it is proposed that key competencies in sustainability should be cultivated integrally. Second, no content element was identified that can promote social participation. Therefore, it is proposed that solutions should be dealt with at the level of social participation. Third, the connection with Sustainable Development Goals(SDGs) was observed in only six of the 28 content elements. Wherever relevant, it is proposed to incorporate key issues related to SDGs. Fourth, the analysis confirmed that only the environmental dimension of sustainable development was considered. Therefore, it is proposed to pursue coexistence based on temporal and spatial relationship and consider the dimensions of environment, society, and economy in an integrated manner.
The purpose of this study was to obtain data necessary for guiding students in the future by grasping which rate of error is made how much depending on a shooting technique in the filming of periapical radiograph. 14,402 films, which were instructed targeting students for the Department of Dental Hygiene at D Health College and S Health College, were analyzed. The following results were obtained by conducting questionnaire survey targeting 263 students who completed the shooting practice lesson of bisecting angle technique and paralleling technique. 1. In case of having shot with bisecting angle technique, the ratio of error was the highest in both maxillary and mandibular first molar. 2. In case of having shot with paralleling technique, the ratio of error was the highest in the maxillary bicuspid and the mandibular first molar. 3. As for ratio of error in light of a shooting technique, both bisecting angle technique and paralleling technique were indicated to be the highest in inaccuracy of film position. The bisecting angle technique was indicated to have the higher ratio of error compared to the paralleling technique. 4. As for ratio of error in light of the processing technique, both bisecting angle technique and paralleling technique were indicated to be the highest in dark image and light image. The bisecting angle technique was indicated to have the higher ratio of error compared to the paralleling technique. 5. Students were indicated to feel it to be most difficult for grasping the processing level in the film-developing process. As the above results, to reduce ratio of error given the periapical radiography, a method of reducing ratio of error given the periapical radiography is considered to be what correctly understands the morphological and anatomical structure inside the mouth and what acquires the shooting technique by filming several times with having enough time.
Journal of The Korean Association of Information Education
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v.16
no.4
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pp.383-395
/
2012
This study investigated the recognition of teachers on the introduction and use of SMART education, which is an ICT-based customized learning method. Through the investigation, the study was to empirically examine the relationship between the use intention of SMART education and its influential factors, and analyze the difference in the use intention of SMART education by teachers, focusing on the experience of SMART education. For analysis purpose, a structural equation model, which was expanded from the theory of reasoned action, was presented. In addition, the difference in path coefficient, which affects the use intention of SMART education, was compared based on the experience with or without SMART education. The results showed that teacher efficacy in the teacher group without SMART education experience was more negatively significant in class burden. In the teacher group with SMART education experience, it was found that the attitude toward SMART education was more significant in use intention; organizational citizenship behavior was more significant in use intention; teacher efficacy was more significant in the attitude toward SMART education and; teacher efficacy was more significant in organizational citizenship behavior. No significant difference was found in the path coefficient among the groups in other hypotheses. Through these results, the factors for introducing and promoting SMART education and its invigoration measures were presented.
This paper focus on the digital media quality of one source multi-use for the vitalization of Korean classical literature. This paper examines the structural relationship between the quality of digital media and learning satisfaction/sustainable use intention through digital media. For this we used the case of The 'Encyclopedia of Korean Local Culture(www.grandculture.net)'. Thus we conducted a survey of 418 high school students attending a classical literature class which used the local culture DB. The result of this study demonstrates that quality of media content and media service quality affect the learning satisfaction even if media system quality does not affect the learning satisfaction. Learning satisfaction affect strongly. The result of multi regression showed that system quality increased the learning satisfaction in the high group, but system quality did not effect the learning satisfaction in the low group. These results indicate that if system quality is enhanced, learning satisfaction will be slightly increased, and if quality of contents and services is enhanced, learning satisfaction will be strongly increased.
Journal of Korean Home Economics Education Association
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v.31
no.3
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pp.73-90
/
2019
The purpose of this study was to explore the differences in perceptions of male and female students about population problems and to draw up implications for population education. Using text mining, the report about population problem, which had written by students in population education class, were analysed. After extracting key words, semantic networks were visualized. The results were as follows. First, the high frequency words were the same for each gender. Second, key words based on frequency did not differ depending on gender. And the key words extracted by the correlation analysis and bigram were different. That is, in the semantic network of girls' words, the network of "life"-"marriage"-"birth"-"pregnancy" appeared independently, distinguishing it from male students who showed separate objective links to population problems. Therefore, it drew suggestions that male and female students should be viewed as heterogeneous groups with different cognitive structures on population problems and that the content and methods of population education should be approached differently depending on gender.
This study aims at analyzing the course selecting standards of college students including Electives and Major courses. For the purpose of this study, we developed a survey through analyzing previous related-research and conducting interviews and pilot study. Using our survey questions, on- and off-line survey was conducted for 301 college students from November 1st to December 5th. As a result of the survey, college student's course selecting standards consist of five factors including 'convenience in learning', 'characteristics of instructors', 'practicality of courses', 'expectations of and interests in learning', and 'external factors.' With the result of factor analysis, we analyzed course selecting standards of college students divided into Electives and Major courses. For electives, 'convenience in learning' was most considered among five factors, followed by 'expectations of and interests in learning', 'external factors', 'characteristics of instructors' and 'practicality of courses.' On the other hand, 'characteristics of instructors' was the highest consideration of college students when selecting courses in Major subjects, followed by 'convenience in learning', 'expectations of and interests in learning', 'external factors' and 'practicality of courses.' This study examined what standards affect college student's course selection, considering Electives and Major courses respectively. We suggested further study on how college students select their courses and how this affect their satisfaction with college education.
Journal of the Korean Society for Library and Information Science
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v.56
no.4
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pp.239-267
/
2022
In this study, the adolescents' perception of the Information Search Process(ISP); their anxiety and the need for intervention during the process were investigated based on Kuhlthau's ISP model, and the relationship among the factors was analyzed with structural equations. In addition, the effectiveness of scaffolding strategy to the experimental group during the class was verified. The main findings are as follows. First, as a result of exploring the anxiety felt by learners and the need for intervention in the information search process, it was found that the presentation stage had a significant effect on the anxiety, and the collection, presentation, and assessment stages had significant effects on the need for intervention. Second, as a result of controlling each variable to investigate the anxiety felt by learners and necessity of intervention in the information search process, it was confirmed that among the demographic variables, the difference between the first graders and the second ones was significant; and the difference in the metropolitan area was more significant than that in the Gangwon province. Third, as a result of paired t-test and covariance analysis on the control group and the experimental group, it was confirmed that the anxiety and need for intervention of the experimental group were statistically significantly reduced. The significance of this study lies in the point that it presents a practical basis for applying scaffolding strategies appropriate to information search process in the field of education.
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