• Korean journal of applied entomology
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• v.47 no.2
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• pp.149-154
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• 2008

Pyrausta panopealis is the major pest in green perilla. The larva weaves a web on the shoot of green perilla and damages. In case of extreme, The larva cuts the main branch of green perilla and the leaf of green perilla isn't harvested anymore. A field study was conducted to estimate economic injury levels (EILs) and control thresholds (CTs) for P. panopealis injuring green perilla in green-houses. Different densities of P. panopealis ranged from 1 to 20 crops (2 units per crop) per 100 crops on 13. June, early inoculation. The number of injured leaf and the rate of injured crop were increased by 23. June, on the other hand were decreased after that day. Also, the amount of yield sow the same result above. The economic loss time calculated by the ratio of cost managing this moth to market price (C/V) (C: cost managing a moth, V: Market price) was 4.0%. The economic injury level was 5.1 larval per 100 crops. The control thresholds calculated by 80% level of economic injury level was 4.1 larval per 100 crops.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.229-236
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• 2009

Cage experiments by artificial infestations with different initial densities of Frankliniella occidentalis were conducted to analyze damages and develop control thresholds of F. occidentalis on greenhouse eggplant in 2005 and on greenhouse sweet pepper in 2007. In the eggplant experiment, the infestations of F. occidentalis resulted in direct damage on fruit surface and non-marketable fruits which had several thin or thick lines or bleaching patches on the surface. F. occidentalis adults were frequently found on the flowers of eggplants, while nymphs were mainly observed on leaves. The fruit yield of eggplants was not significantly different among experimental plots with different initial density of F. occidentalis. Relationship between % non-marketable fruits among harvested fruits of eggplant and sticky trap catches of F. occidentalis (no. thrips/trap/week) at two weeks before the harvest showed a positive correlation. Using the estimated relationship, the control threshold of F. occidentalis on greenhouse eggplant was estimated at 10 adults per week at two weeks before the harvest when 5% of non-marketable fruit was applied for the gain threshold. In the experiment of sweet pepper, the direct damage by F. occidentalis was observed on the fruit surface and calyx, and the marketable grade of the damaged fruits decreased. The significant yield loss of marketable fruits was found in plots with high initial introduced-densities. There was a high relationship between thrips density and percentage of damaged fruits. Assuming 5% yield loss (non-marketable fruit) for the gain threshold, the control threshold of F. occidentalis on greenhouse sweet pepper was 4.8 adults per trap and 0.9 individuals per flower at two weeks before harvest.

• Korean journal of applied entomology
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• v.48 no.1
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• pp.29-35
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• 2009

Three lepidopteran insect pests of the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), the soybean pod worm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and an Ostrinia spp. (Lepidoptera: Pyralidae) attacking adzuki bean, Vigna angularis, were confirmed as the major insect pests during the reproductive developmental stage of adzuki bean, and M. vitrata existed dominantly. Almost all plants in the adzuki bean field were injured by at least one of the three species, and $15{\sim}60%$ of reproductive organs were injured. Unbloomed inflorescence occurred continuously through the reproductive stage of adzuki bean. While the flower was many at the early stage and its number soon decreased, the pod was few at the early stage, but its number increased soon and maintained at a constant level. The results suggested that the adzuki bean injured by insect pests compensate the flower loss by developing new inflorescence, but the compensation do not cause the development of new pods. While M. vitrata and M. phaseoli were observed in flowers, pods and stem mainly during the first half of reproductive stage of adzuki bean, Ostrinia spp. was observed only in pods and stem during the second half. In addition, while all instars of larvae of M. vitrata were observed, larvae between the third and fifth instars for M. pahseoli and Ostrinia spp. were observed.

• Journal of the korean academy of Pediatric Dentistry
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• v.47 no.3
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• pp.320-326
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• 2020

The aim of this study was to compare compressive strength and microhardness of recently introduced alkasite restorative materials with glass ionomer cement and flowable composite resin. For each material, 20 samples were prepared respectively for compressive strength and Vickers microhardness test. The compressive strength was measured with universal testing machine at crosshead speed of 1 mm/min. And microhardness was measured using Vickers Micro hardness testing machine under 500 g load and 10 seconds dwelling time at 1 hour, 1 day, 7 days, 14 days, 21 days and 35 days. The compressive strength was highest in composite resin, followed by alkasite, and glass ionomer cement. In microhardness test, composite resin, which had no change throughout experimental periods, showed highest microhardness in 1 hour, 1 day, and 7 days measurement. The glass ionomer cement showed increase in microhardness for 7 days and no difference was found with composite resin after 14 days measurement. For alkasite, maximum microhardness was measured on 14 days, but showed gradual decrease.

• Korean Journal of Organic Agriculture
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• v.17 no.2
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• pp.227-236
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• 2009

This research was carried out to investigate the seasonal occurrence of major pests and the effects of releases of natural enemies for biological control of the major pests in eggplant greenhouse. A total of 8 pest species in 7 families were identified. Among these pests, Myzus persicae, Aphis gossypii, Frankliniella occidentalis, Tetranychus urticae and Trialeurodes vaporariorum were the dominant species in eggplant greenhouse. Two aphid species occurred mainly from May to June, but their populations decreased rapidly from July. The population density of F. occidentalis was high from June to July and T. urticae and T. vaporariorum were abundant from July to September during the growing season of eggplant. In the trials of biological control of pests, aphids could be suppressed within the range of $87{\sim}97%$ by two times releases of Chrysopa pallens eggs. F. occidentalis and T. urticae could be suppressed within the range of $76{\sim}90\;and\;87{\sim}91%$ by three times releases of Orius sauteri adults, respectively. The releases of C. pallens eggs and O. sauteri adults were as effective as three times applications of insecticides for the control of aphids, F occidentalis and T. urticae from May to September.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.231-237
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• 2014

This study was carried out to investigate the pest species and their damage on Asparagus officinalis. Field surveys were conducted in asparagus greenhouse in Jeju, for four years from 2007 to 2010. Total of 16 pest species belonging to 5 orders were found as follows: Thrips tabaci, Thrips hawaiiensis, Adoxophyes honmai, Spodoptera litura, Spodoptera exigua, Helicoverpa armigera, Mamestra brassicae, Ascotis selenaria, Ectropis excellens, Geisha distinctissima, Aphis gossypii, Myzus persicae, Anomala albopilosa, Crioceris quatuordecimpunctata, Acusta despecta sieboldiana and Deroceras reticulatum. Among them, T. tabaci frequently occurred and damaged asparagus plants during the whole growing seasons. C. quatuordecimpunctata and snails caused severe damages on asparagus shoots in spring harvest season. In mid-late seasons, aphids and lepidopteran species occurred and attacked asparagus plants. Of the lepidopteran pests, S. litura, S exigua and H. armigera belonging to Noctuidae occurred moderately and fed on asparagus plants. And the pest control strategy in asparagus cultivation was discussed based on the present results.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.351-355
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• 2010

This study was conducted to determine the relationship of larval density of tobacco cutworm, Spodoptera litura (Fabricius) to damage in greenhouse sweet pepper. Laboratory experiments, cage experiments by artificial release and field investigation were carried out in 2008. The leaf consumption rate increased greatly with larval development. The damaged leaves had several round or oval shape holes on the surface or lost certain parts of them, and the fruit damaged had a conspicuous hole on the surface or scar marks around the calyx. In the field investigation, fruit damage was highly correlated with larval densities and reached 3.5% damage at maximum. Cage experiments showed that numbers of non-marketable fruit increased as increasing larval densities released. The larval density at two weeks before harvest had a high relationship with the percentage of damaged-fruit at harvest. Corresponding larval density caused 1, 3, 5% of damaged-fruit was 0.2, 0.5, 0.8 larvae per plant, respectively.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.175-182
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• 2019

The citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillaridae), is an important pest that decreases the productivity of citrus trees by attacking citrus leaves, and especially it causes a severe damage during the young growth stage. In this study, we investigated the distribution of eggs on the leaves of shoots according to the position and the survival rate of this pest in the field condition. The occurrence patterns of P. citrella eggs in citrus orchards were largely affected by the occurrence of shoots. Even if the adults were still being attracted in the pheromone traps, the eggs were not observed on leaves when the growth of shoots was stopped. The density of P. citrella eggs in the citrus shoots peaked at the fifth or sixth leaf from the terminal leaf, and over 90% of the eggs were found in the first to eighth leaves as a whole. In 2015 and 2016, the average survival rate of larvae surviving to pupa was 1.4%, and the rate of survival to adults was 0.2%, indicating that most of them died. Furthermore, we have discussed the management strategy of P. citrella considering the distribution of eggs and the larval survival rate in the citrus shoots.

• Korean Journal of Microbiology
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• v.49 no.1
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• pp.64-70
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• 2013

The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors. It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic identification using sequences of the ITS, ${\beta}$-tubulin, and EF1-${\alpha}$ regions. This fungus was named as I. fumosorosea SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the virulence increased with increasing conidial concentrations, $1{\times}10^5$ to $10^8$ conidia/ml. Conidia were stable at $35^{\circ}C$, 0.1 $J/cm^2$ of UV irradiation and germinated after 8 days at $4^{\circ}C$. Additionally, the activities of chitinases and proteases produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management programs against the greenhouse whitefly.

• Applied Microscopy
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• v.38 no.4
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• pp.307-314
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• 2008

Hydrophytes such as Spirodela polyrhiza form dormant turions to withstand cold winters. The turion is an anatomically distinct structure from which a vegetative frond arises later during germination. The turions sink to the bottom of the pond when temperatures drop and remain there throughout the winter. In the spring, they float to the surface and germinate into a new frond from the turion primordium. Unlike fronds, turions are known to possess small aerenchyma, starch grains, and relatively dense cytoplasm. These features allow the turions to survive the cold winter season at the bottom of the pond. Spirodela polyrhiza has been investigated previously to a great extent, especially in its physiological, biochemical and ecological attributes. However, a little is known about the structural features of the frond and turion during turion development. Thus, the aim of the present study was to reveal the structural characteristics of the frond and turion with regard to tissue differentiation, aerenchyma development, starch distribution, and ultrastructure, with the use of electron microscopy. A moderate degree of mesophyll tissue differentiation was found in the frond, whereas the turion did not exhibit such differentiation. Within the frond tissue, approximately $37{\sim}45%$ of the cellular volume was occupied by a large aerenchyma, but only $9{\sim}15%$ was taken up by the aerenchyma in the turion. The turion cells, especially those of the turion primordium, were derived from frond cells, and contained cytoplasm. Their cytoplasm was densely packed with plastids, mitochondria, endoplasmic reticulum, Golgi bodies, and microtubules. Plasmodesmata were also well developed within these cells. The most striking feature observed was the distribution of starch grains within the plastids of turion cells. Before the turion sank to the bottom of the pond, a considerable amount of starch accumulated in the plastid stroma. The starch grains dissolved when temperatures rose in the spring, and this promptly provided the nutrients which the primordium needed for turion germination. The turion therefore, was an appropriate dormant structure for free-floating, reduced hydrophytes like Spirodela polyhriza due to its small aerenchyma and large starch grains that aided in the purpose of sinking below the surface of the water to survive cold winters. The new fronds that arose from such turions grew rapidly in the spring, beginning the new life cycle.