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Probe-based qPCR Assay for Rapid Detection of Predominant Candida glabrata Sequence Type in Korea

  • Bae, Jinyoung;Lee, Kyung Eun;Jin, Hyunwoo
    • Biomedical Science Letters
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    • v.25 no.4
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    • pp.407-416
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    • 2019
  • Recent years have seen an increase in the incidence of candidiasis caused by non-albicans Candida (NAC) species. In fact, C. glabrata is now second only to C. albicans as the most common cause of invasive candidiasis. Therefore, the rapid genotyping specifically for C. glabrata is required for early diagnosis and treatment of candidiasis. A number of genotyping assays have been developed to differentiate C. glabrata sequence types (STs), but they have several limitations. In the previous study, multi-locus sequence typing (MLST) has performed with a total of 101 C. glabrata clinical isolates to analyze the prevalent C. glabrata STs in Korea. A total of 11 different C. glabrata STs were identified and, among them, ST-138 was the most commonly classified. Thus, a novel probe-based quantitative PCR (qPCR) assay was developed and evaluated for rapid and accurate identification of the predominant C. glabrata ST-138 in Korea. Two primer pairs and hybridization probe sets were designed for the amplification of internal transcribed spacer 1 (ITS1) region and TRP1 gene. Analytical sensitivity of the probe-based qPCR assay was 100 ng to 10 pg and 100 ng to 100 pg (per 1 μL), which target ITS1 region and TRP1 gene, respectively. This assay did not react with any other Candida species and bacteria except C. glabrata. Of the 101 clinical isolates, 99 cases (98%) were concordant with MLST results. This novel probe-based qPCR assay proved to be rapid, sensitive, highly specific, reproducible, and cost-effective than other genotyping assay for C. glabrata ST-138 identification.

BERTRAND CURVES IN NON-FLAT 3-DIMENSIONAL (RIEMANNIAN OR LORENTZIAN) SPACE FORMS

  • Lucas, Pascual;Ortega-Yagues, Jose Antonio
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.4
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    • pp.1109-1126
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    • 2013
  • Let $\mathbb{M}^3_q(c)$ denote the 3-dimensional space form of index $q=0,1$, and constant curvature $c{\neq}0$. A curve ${\alpha}$ immersed in $\mathbb{M}^3_q(c)$ is said to be a Bertrand curve if there exists another curve ${\beta}$ and a one-to-one correspondence between ${\alpha}$ and ${\beta}$ such that both curves have common principal normal geodesics at corresponding points. We obtain characterizations for both the cases of non-null curves and null curves. For non-null curves our theorem formally agrees with the classical one: non-null Bertrand curves in $\mathbb{M}^3_q(c)$ correspond with curves for which there exist two constants ${\lambda}{\neq}0$ and ${\mu}$ such that ${\lambda}{\kappa}+{\mu}{\tau}=1$, where ${\kappa}$ and ${\tau}$ stand for the curvature and torsion of the curve. As a consequence, non-null helices in $\mathbb{M}^3_q(c)$ are the only twisted curves in $\mathbb{M}^3_q(c)$ having infinite non-null Bertrand conjugate curves. In the case of null curves in the 3-dimensional Lorentzian space forms, we show that a null curve is a Bertrand curve if and only if it has non-zero constant second Frenet curvature. In the particular case where null curves are parametrized by the pseudo-arc length parameter, null helices are the only null Bertrand curves.

Application of Software Quality Model and Metric for Software Product Assurance for KASS Control Station (KASS 통합운영국 소프트웨어 품질 보증을 위한 소프트웨어 품질 모델 및 메트릭 적용방안)

  • Kim, Youn-sil;Lee, Eun-sung
    • Journal of Advanced Navigation Technology
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    • v.24 no.1
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    • pp.28-36
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    • 2020
  • Korea augmentation satellite system (KASS) is the Korean satellite based augmentation system (SBAS) developed by ministry of land, infrastructure, and transport (MOLIT) since 2014. Since KASS is the safety critical system that can affect to the safety of airplane, the software of KASS is developed according to the DO178B software level induced from safety analysis. In case of KASS control station (KCS), most of the software of KCS get assigned software level E in DO178B. In that case, ECSS-Q-ST-80C category D is assigned as a software product assurance standard. In this paper, the software related standard ECSS-E-ST-40C, ECSS-Q-HB-80-04A are analyzed to satisfy ECSS-Q-ST-80C and as a result the software product assurance activities regarding software life cycle and the software quality model, metric is proposed for the product assurance of the KCS software.

Characteristics of a New Obligate Methanol-Oxidizing Bacterium (새로운 절대 메탄올 산화세균의 분리 및 특성)

  • Kim, Si-Wook;Park, Yong-Ha
    • Korean Journal of Microbiology
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    • v.31 no.4
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    • pp.261-266
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    • 1993
  • A new methyltrophic bacterium which utilizes methanol as a sole source of carbon and energy was isolated from soil. It was Gram-negative, nonmotile, nonspore-forming rod, and strictly aerobic bacterium. Catalase and oxidase activities were present. Nitrate was reduced to nitrite. Vitamins and other growth factors were not required. Generation time was 1.6 hr under the optimal condition. The isolate assimilated methanol via the ribulose mono-phosphate pathway (Enter-Doudoroff varient) and did not have .alpha.-ketoglutarate dehydrogenase. It assimilated ammonia through glutamate dehydrogenase. The guanine plus cytosine content of the DNA was 61.0 mol%. The celular fatty acid composition was primarily straight-chain saturated $C^{16 : 0}$ acids (palmitic acids) and unsaturated $C_{16 :1}$ acid (palmitoleic acids), and the isolate also contained two unidentified $C_{17}$ branched fatty acids. The major ubiquinone was Q-8, and Q-6 and Q-7 were present as minor components. Phosphatidylethanolamine and phosphatidylglycerol were predominantly present, and diphosphatidyglycerol was also detected. Based on the physiological and biochemical properties, the isolate was assigned to a novel species of the genus Methylobacillus, Methylobacillus methanolovorus sp. nov.

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A Study on the Determination of Construction Depth of Vertical Drain by Cone Resistance (콘 관입저항치를 이용한 수직배수재 타설심도 결정에 관한 연구)

  • Kim, Yeon-Jung;Kim, Nam-Ho;Shin Yun-Sup
    • 기술발표회
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    • s.2006
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    • pp.261-269
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    • 2006
  • Recently, piezocone penetration test is frequently used in order to estimate the characteristics of soft ground with standard penetration test; generally used in the past In this study, standard penetration test, piezocone penetration test, driving resistance of vertical drain were used in order to increase the confidence for determination of soft ground depth. And the compressible layer was determined by the comparison between the preconsolidation pressure and the designed increase pressure. As the results, the relation between standard penetration test and piezocone penetration test shows $q_c$=(1.09~1.63)N at the soft ground, determined by 5/30 N value. And $q_c$(1.21~1.98)N was shown at the point of compressible layer, evaluated by the preconsolidation pressure. And driving resistance of vertical drain is 70 f/$cm^2$ which is equal to 10kgf/$cm^2$ cone penetration resistance.

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PROPERTIES ON q-DIFFERENCE RICCATI EQUATION

  • Huang, Zhi-Bo;Zhang, Ran-Ran
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.6
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    • pp.1755-1771
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    • 2018
  • In this paper, we investigate a certain type of q-difference Riccati equation in the complex plane. We prove that q-difference Riccati equation possesses a one parameter family of meromorphic solutions if it has three distinct meromorphic solutions. Furthermore, we find that all meromorphic solutions of q-difference Riccati equation and corresponding second order linear q-difference equation can be expressed by q-gamma function if this q-difference Riccati equation admits two distinct rational solutions and $q{\in}{\mathbb{C}}$ such that 0 < ${\mid}q{\mid}$ < 1. The growth and value distribution of differences of meromorphic solutions of q-difference Riccati equation are also treated.

NOTES ON THE SPACE OF DIRICHLET TYPE AND WEIGHTED BESOV SPACE

  • Choi, Ki Seong
    • Journal of the Chungcheong Mathematical Society
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    • v.26 no.2
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    • pp.393-402
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    • 2013
  • For 0 < $p$ < ${\infty}$, ${\alpha}$ > -1 and 0 < $r$ < 1, we show that if $f$ is in the space of Dirichlet type $\mathfrak{D}^p_{p-1}$, then ${\int}_{1}^{0}M_{p}^{p}(r,f^{\prime})(1-r)^{p-1}rdr$ < ${\infty}$ and ${\int}_{1}^{0}M_{(2+{\alpha})p}^{(2+{\alpha})p}(r,f^{\prime})(1-r)^{(2+{\alpha})p+{\alpha}}rdr$ < ${\infty}$ where $M_p(r,f)=\[\frac{1}{2{\pi}}{\int}_{0}^{2{\pi}}{\mid}f(re^{it}){\mid}^pdt\]^{1/p}$. For 1 < $p$ < $q$ < ${\infty}$ and ${\alpha}+1$ < $p$, we show that if there exists some positive constant $c$ such that ${\parallel}f{\parallel}_{L^{q(d{\mu})}}{\leq}c{\parallel}f{\parallel}_{\mathfrak{D}^p_{\alpha}}$ for all $f{\in}\mathfrak{D}^p_{\alpha}$, then ${\parallel}f{\parallel}_{L^{q(d{\mu})}}{\leq}c{\parallel}f{\parallel}_{\mathcal{B}_p(q)}$ where $\mathcal{B}_p(q)$ is the weighted Besov space. We also find the condition of measure ${\mu}$ such that ${\sup}_{a{\in}D}{\int}_D(k_a(z)(1-{\mid}a{\mid}^2)^{(p-a-1)})^{q/p}d{\mu}(z)$ < ${\infty}$.

An Experimental Study on the Oxidation Process of Silicon (실리콘 산화공정에 대한 실험적 고찰)

  • 최연익;김충기
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.16 no.1
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    • pp.26-32
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    • 1979
  • Dry oxidation and wet oxidation processes of silicon have been examined experimentally. The oxidation temperatures were 1.10$0^{\circ}C$, 1.15$0^{\circ}C$, and 1.200 $^{\circ}C$, and oxygen flow rate was changed from 0.2 liter/min to 2.8 liter/min. From the experimental measurements, oxidation temperaturel time and oxygen flow rate have been tabutated for oxide layers 0.1$\mu$ - 1.0$\mu$ in thickness. The quality of the grown oxide layer has been investigated In terms of the dielectric constant, breakdown voltage, fixed surface charge densify (Qss/q) and mobile charge density (Q /q). From these measurements, it is concluded that the quality of the oxide layer is sufficient to expect the normal operation of MOS transistors.

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A Study on Unit Treatment Cost of Sewage Disposal Plant in the Service Area under Highway (생물학적 처리시설의 처리비 원단위 산정에 관한 연구 -고속도로 휴게소를 중심으로-)

  • 장철현;박상우;홍태석
    • Journal of Environmental Science International
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    • v.11 no.6
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    • pp.537-541
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    • 2002
  • This study aimed to obtain the relative formula with the unit treatment cost according to the treatment of a sewage plant in the service area under highway. The following results were obtained. The correlative formula connected to amount of sewage(Q)generation was as follows ; between an annual amount of sale(C) showed Q=19.113$.$C$\^$0.9294/, and between the number of users(P) showed Q=2${\times}$10$\^$-8/ $.$P$^2$- 0.0298$.$P + 75,666. The correlative formula connected to the treatment cost was as follows , according to the amount of sewage generation showed S= 3${\times}$10$\^$-6/$.$Q 0-0.2266$.$Q+29,895, according to the elimination of BOD(E) showed S=6${\times}$10$\^$-5/$.$E$^2$-0.6717$.$E + 27,744, according to the annual amount of sale showed S=0.0005 C$^2$-4.8013$.$C + 35,118, with the number, of persons(P) using the service area showed S= 2${\times}$10$\^$-8/ $.$P$^2$- 0.046$.$P + 48,803.

Enhanced Q-Algorithm for Fast Tag Identification in EPCglobal Class-1 Gen-2 RFID System (EPCglobal Class-1 Gen-2 RFID 시스템에서 고속 태그 식별을 위한 개선된 Q-알고리즘)

  • Lim, In-Taek
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.3
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    • pp.470-475
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    • 2012
  • In Q-algorithm of EPCglobal Class-1 Gen-2 RFID system, the initial value of $Q_{fp}$, which is the slot-count parameter, is not defined in the standard. And the values of weight C, which is the parameter for incrementing or decrementing the slot-count size, are not determined. Therefore, if the number of tags is small and we let the initial $Q_{fp}$ be large, the number of empty slot will be large. On the other hand, if we let the initial $Q_{fp}$ be small in spite of many tags, almost all the slots will be collided. Also, if the reader selects an inappropriate weight, there are a lot of empty or collided slots. As a result, the performance will be declined because the frame size does not converge to the optimal point quickly during the query round. In this paper, we propose a scheme to allocate the optimal initial $Q_{fp}$ through the tag number estimation and select the weight based on the slot-count size of current query round.