• 제목/요약/키워드: $\sigma$54

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P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $\sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators

  • Kim, Euhgbin;Kim, Daeyou;Lee, Joon-Haeng
    • Journal of Microbiology
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    • 제38권3호
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    • pp.176-179
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    • 2000
  • A challenge phage system was used to study the DNA-protein interaction between C4-dicarboxylic acid transport protein D(DCTD) or $\sigma$54, and a $\sigma$54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or $\sigma$54 directed this challenge phage towards lysogency, indicating that DCTD or E$\sigma$54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD(or $\sigma$54) and the dctA promoter region.

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P22-Based Challenge Phage Constructs to Study Protein-Protein Interactions between the $\sigma$$^{54}$-Dependent Promoter, dctA, and Its Transcriptional Regulators

  • Song, Jeong-Min;Kim, Eungbin;Lee, Joon H.
    • Journal of Microbiology
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    • 제40권3호
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    • pp.205-210
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    • 2002
  • To study interactions between $C_{4}$-dicarboxylic acid transport protein D and E$\sigma$$^{54}$ in the dctA promoter regulatory region, we used the challenge phage system. An ant'-`lac fusion was recombined onto the challenge phage, and this ant'-`lac fusion along with Pant and the R. meliloti dctA promoter regulatory region were cloned onto a plasmid. The plasmid bearing the ant'-`lac fusion was used as a reporter plasmid in a coupled transcription-translation system. Addition of purified $\sigma$$^{54}$ to the coupled system specifically repressed transcription of the plasmid-borne ant'-`lac fusion. When DCTD was added along with $\sigma$$^{54}$ to the coupled system, transcription of the ant'-`lac fusion was even further repressed, suggesting that DCTD may stabilize closed complexes between E$\sigma$$^{54}$ and the dctA promoter.

Confidence Intervals in Three-Factor-Nested Variance Component Model

  • Kang, Kwan-Joong
    • Journal of the Korean Statistical Society
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    • 제22권1호
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    • pp.39-54
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    • 1993
  • In the three-factor nested variance component model with equal numbers in the cells given by $y_{ijkm} = \mu + A_i + B_{ij} + C_{ijk} + \varepsilon_{ijkm}$, the exact confidence intervals of the variance component of $\sigma^2_A, \sigma^2_B, \sigma^2_C, \sigma^2_{\varepsilon}, \sigma^2_A/\sigma^2_{\varepsilon}, \sigma^2_B/\sigma^2_{\varepsilon}, \sigma^2_C/\sigma^2_{\varepsilon}, \sigma^2_A/\sigma^2_C, \sigma^2_B/\sigma^2_C$ and $\sigma^2_A/\sigma^2_B$ are not found out yet. In this paper approximate lower and upper confidence intervals are presented.

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Rhizobium meliloti와 R. leguminosarum 의 dctA 프로모터에서 DctD 및 NtrC가 중재된 초 in vitro 전사활성 (DctD- or NtrC-mediated in vitro Transcriptional Activation from Rhizobium meliloti and R. leguminosarum dctA Promoter)

  • 최상기;이준행
    • 한국미생물·생명공학회지
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    • 제32권2호
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    • pp.190-194
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    • 2004
  • The gene product of dctD (DctD) activates transcription from the dctA promoter regulatory region by the $\sigma^{54}$ -holoenzyme form ofRNA polymerase ($E\sigma^{54}$ ) in Rhizobium meliloti and R. leguminosarum. The Escherichia coli integration host factor (IHF) stimulated DctD-mediated activation from the dctA promoter regulatory region of R. leguminosarum but not R. meliloti. In the absence of UAS, IHF inhibited DctD-mediated activation from both of these promoter regulatory regions. IHF also inhibited activation from R. leguminosarum dctA by nitrogen regulatory protein C (NtrC), another activator of $E\sigma^{54}$ but not by one which lacks a specific binding site in this promoter regulatory region. IHF, however, stimulated NtrC-mediated activation from the R. meliloti dctA promoter. Upon removal of the UAS, IHF inhibited NtrC-mediated transcription activation from the R. meliloti dctA promoter regulatory region. These data suggest that IHF likely faciliates productive contacts between the activators NtrC or DctD and $E\sigma^{54}$ to stimulate activation from dctA promoter.

Redox-Dependent Modulation of RsrA, an Anti-Sigma Factor Regulating Thioredoxin Operons in Streptomyces coelicolor

  • Bae, Jae-Bum;Park, Joo-Hong;Roe, Jung-Hye
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 2002년도 제9회 학술 발표회 프로그램과 논문초록
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    • pp.54-54
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    • 2002
  • $\sigma$$\^$R/ is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in Streptomyces coelicolor. RsrA, an anti-sigma factor, specifically binds to $\sigma$$\^$R/ and inhibits $\sigma$$\^$R/-directed transcription under reducing conditions. Exposure to H$_2$O$_2$ or thiol-specific oxidant diamide dissociates $\sigma$$\^$R/-RsrA complex. The redox-dependent regulation of $\sigma$$\^$R/-RsrA binding has been reported to involve thiol-disulfide exchange in RsrA, which contains 7 cysteines in 105 amino acid residues.(omitted)

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GENERALIZED BROWDER, WEYL SPECTRA AND THE POLAROID PROPERTY UNDER COMPACT PERTURBATIONS

  • Duggal, Bhaggy P.;Kim, In Hyoun
    • 대한수학회지
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    • 제54권1호
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    • pp.281-302
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    • 2017
  • For a Banach space operator $A{\in}B(\mathcal{X})$, let ${\sigma}(A)$, ${\sigma}_a(A)$, ${\sigma}_w(A)$ and ${\sigma}_{aw}(A)$ denote, respectively, its spectrum, approximate point spectrum, Weyl spectrum and approximate Weyl spectrum. The operator A is polaroid (resp., left polaroid), if the points $iso{\sigma}(A)$ (resp., $iso{\sigma}_a(A)$) are poles (resp., left poles) of the resolvent of A. Perturbation by compact operators preserves neither SVEP, the single-valued extension property, nor the polaroid or left polaroid properties. Given an $A{\in}B(\mathcal{X})$, we prove that a sufficient condition for: (i) A+K to have SVEP on the complement of ${\sigma}_w(A)$ (resp., ${\sigma}_{aw}(A)$) for every compact operator $K{\in}B(\mathcal{X})$ is that ${\sigma}_w(A)$ (resp., ${\sigma}_{aw}(A)$) has no holes; (ii) A + K to be polaroid (resp., left polaroid) for every compact operator $K{\in}B(\mathcal{X})$ is that iso${\sigma}_w(A)$ = ∅ (resp., $iso{\sigma}_{aw}(A)$ = ∅). It is seen that these conditions are also necessary in the case in which the Banach space $\mathcal{X}$ is a Hilbert space.

APPLICATION OF CONVOLUTION SUM ∑k=1N-1σ1(k)σ1(2nN-2nk)

  • Kim, Daeyeoul;Kim, Aeran
    • Journal of applied mathematics & informatics
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    • 제31권1_2호
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    • pp.45-54
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    • 2013
  • Let $$S^{\pm}_{(n,k)}\;:=\{(a,b,x,y){\in}\mathbb{N}^4:ax+by=n,x{\equiv}{\pm}y\;(mod\;k)\}$$. From the formula $\sum_{(a,b,x,y){\in}S^{\pm}_{(n,k)}}\;ab=4\sum_{^{m{\in}\mathbb{N}}_{m<n/k}}\;{\sigma}_1(m){\sigma}_1(n-km)+\frac{1}{6}{\sigma}_3(n)-\frac{1}{6}{\sigma}_1(n)-{\sigma}_3(\frac{n}{k})+n{\sigma}_1(\frac{n}{k})$, we find the Diophantine solutions for modulo $2^{m^{\prime}}$ and $3^{m^{\prime}}$, where $m^{\prime}{\in}\mathbb{N}$.

수동형 적분기(Passive Integrator)를 이용한 저전력 이산시간 Incremental Delta Sigma ADC (Low Power Discrete-Time Incremental Delta Sigma ADC with Passive Integrator)

  • 오군석;김진태
    • 전자공학회논문지
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    • 제54권1호
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    • pp.26-32
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    • 2017
  • 본 논문에서는 설계 요구가 높고, 전력 소모가 높은 opamp를 이용하는 기존의 능동형 적분기를, 수동형 적분기로 대체하여 고속의 저전력, 고해상도 특성을 갖는 incremental delta-sigma ADC를 소개한다. 능동형 적분기에서 수동형 적분기로의 변환을 위해, 기존의 능동형 적분기의 특성을 분석하였다. 이를 바탕으로 opamp의 설계 요구를 낮추고, 더 나아가 opamp를 사용하지 않는 저전력의 수동형 적분기를 제안하였다. 65nm 공정을 이용하여 수동형 적분기로 구성된 1차 single-bit incremental delta-sigma ADC를 설계하였다. Transistor-level 시뮬레이션 결과, 이는 supply 전압이 1.2V인 상황에서 modulator만 0.6uW, digital filter를 포함한 ADC 전체에서 6.25uW를 소모하며 BW 22KHz, SNDR 71dB, dynamic range 74.6dB을 달성하였다.

Lean 6 Sigma에 의한 LCD TV의 Modular Cell Line 구축에 관한 연구 (Study on Construction of Modular Cell Line for LCD TV by Lean 6 Sigma)

  • 정영관;최성대;유종규;정선환
    • 한국산업융합학회 논문집
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    • 제13권1호
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    • pp.49-54
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    • 2010
  • Lean 6 sigma has recently been used to describe a management system which combines lean management and 6 sigma. The marriage between Lean manufacturing and 6 sigma has proven to be a powerful tool for cutting waste and improving the organization operations. Time and quality are the most important metrics in improving any company's production and profit performance. lean 6 sigma is a management innovation for improving production efficiency, process quality, cost reduction, investment efficiency and customer's satisfaction. in this paper, Advanced cell line is builded the home appliance goods of the LCD TV final assembly line of domestic company line, training the multi-skilled man and controlling the production information system based on Lean 6 sigma.

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