• Title/Summary/Keyword: $\sigma$$^{54}$

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P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $\sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators

  • Kim, Euhgbin;Kim, Daeyou;Lee, Joon-Haeng
    • Journal of Microbiology
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    • v.38 no.3
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    • pp.176-179
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    • 2000
  • A challenge phage system was used to study the DNA-protein interaction between C4-dicarboxylic acid transport protein D(DCTD) or $\sigma$54, and a $\sigma$54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or $\sigma$54 directed this challenge phage towards lysogency, indicating that DCTD or E$\sigma$54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD(or $\sigma$54) and the dctA promoter region.

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P22-Based Challenge Phage Constructs to Study Protein-Protein Interactions between the $\sigma$$^{54}$-Dependent Promoter, dctA, and Its Transcriptional Regulators

  • Song, Jeong-Min;Kim, Eungbin;Lee, Joon H.
    • Journal of Microbiology
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    • v.40 no.3
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    • pp.205-210
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    • 2002
  • To study interactions between $C_{4}$-dicarboxylic acid transport protein D and E$\sigma$$^{54}$ in the dctA promoter regulatory region, we used the challenge phage system. An ant'-`lac fusion was recombined onto the challenge phage, and this ant'-`lac fusion along with Pant and the R. meliloti dctA promoter regulatory region were cloned onto a plasmid. The plasmid bearing the ant'-`lac fusion was used as a reporter plasmid in a coupled transcription-translation system. Addition of purified $\sigma$$^{54}$ to the coupled system specifically repressed transcription of the plasmid-borne ant'-`lac fusion. When DCTD was added along with $\sigma$$^{54}$ to the coupled system, transcription of the ant'-`lac fusion was even further repressed, suggesting that DCTD may stabilize closed complexes between E$\sigma$$^{54}$ and the dctA promoter.

Confidence Intervals in Three-Factor-Nested Variance Component Model

  • Kang, Kwan-Joong
    • Journal of the Korean Statistical Society
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    • v.22 no.1
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    • pp.39-54
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    • 1993
  • In the three-factor nested variance component model with equal numbers in the cells given by $y_{ijkm} = \mu + A_i + B_{ij} + C_{ijk} + \varepsilon_{ijkm}$, the exact confidence intervals of the variance component of $\sigma^2_A, \sigma^2_B, \sigma^2_C, \sigma^2_{\varepsilon}, \sigma^2_A/\sigma^2_{\varepsilon}, \sigma^2_B/\sigma^2_{\varepsilon}, \sigma^2_C/\sigma^2_{\varepsilon}, \sigma^2_A/\sigma^2_C, \sigma^2_B/\sigma^2_C$ and $\sigma^2_A/\sigma^2_B$ are not found out yet. In this paper approximate lower and upper confidence intervals are presented.

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DctD- or NtrC-mediated in vitro Transcriptional Activation from Rhizobium meliloti and R. leguminosarum dctA Promoter (Rhizobium meliloti와 R. leguminosarum 의 dctA 프로모터에서 DctD 및 NtrC가 중재된 초 in vitro 전사활성)

  • 최상기;이준행
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.190-194
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    • 2004
  • The gene product of dctD (DctD) activates transcription from the dctA promoter regulatory region by the $\sigma^{54}$ -holoenzyme form ofRNA polymerase ($E\sigma^{54}$ ) in Rhizobium meliloti and R. leguminosarum. The Escherichia coli integration host factor (IHF) stimulated DctD-mediated activation from the dctA promoter regulatory region of R. leguminosarum but not R. meliloti. In the absence of UAS, IHF inhibited DctD-mediated activation from both of these promoter regulatory regions. IHF also inhibited activation from R. leguminosarum dctA by nitrogen regulatory protein C (NtrC), another activator of $E\sigma^{54}$ but not by one which lacks a specific binding site in this promoter regulatory region. IHF, however, stimulated NtrC-mediated activation from the R. meliloti dctA promoter. Upon removal of the UAS, IHF inhibited NtrC-mediated transcription activation from the R. meliloti dctA promoter regulatory region. These data suggest that IHF likely faciliates productive contacts between the activators NtrC or DctD and $E\sigma^{54}$ to stimulate activation from dctA promoter.

Redox-Dependent Modulation of RsrA, an Anti-Sigma Factor Regulating Thioredoxin Operons in Streptomyces coelicolor

  • Bae, Jae-Bum;Park, Joo-Hong;Roe, Jung-Hye
    • Proceedings of the Korean Biophysical Society Conference
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    • 2002.06b
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    • pp.54-54
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    • 2002
  • $\sigma$$\^$R/ is a sigma factor responsible for inducing the thioredoxin system in response to oxidative stress in Streptomyces coelicolor. RsrA, an anti-sigma factor, specifically binds to $\sigma$$\^$R/ and inhibits $\sigma$$\^$R/-directed transcription under reducing conditions. Exposure to H$_2$O$_2$ or thiol-specific oxidant diamide dissociates $\sigma$$\^$R/-RsrA complex. The redox-dependent regulation of $\sigma$$\^$R/-RsrA binding has been reported to involve thiol-disulfide exchange in RsrA, which contains 7 cysteines in 105 amino acid residues.(omitted)

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GENERALIZED BROWDER, WEYL SPECTRA AND THE POLAROID PROPERTY UNDER COMPACT PERTURBATIONS

  • Duggal, Bhaggy P.;Kim, In Hyoun
    • Journal of the Korean Mathematical Society
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    • v.54 no.1
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    • pp.281-302
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    • 2017
  • For a Banach space operator $A{\in}B(\mathcal{X})$, let ${\sigma}(A)$, ${\sigma}_a(A)$, ${\sigma}_w(A)$ and ${\sigma}_{aw}(A)$ denote, respectively, its spectrum, approximate point spectrum, Weyl spectrum and approximate Weyl spectrum. The operator A is polaroid (resp., left polaroid), if the points $iso{\sigma}(A)$ (resp., $iso{\sigma}_a(A)$) are poles (resp., left poles) of the resolvent of A. Perturbation by compact operators preserves neither SVEP, the single-valued extension property, nor the polaroid or left polaroid properties. Given an $A{\in}B(\mathcal{X})$, we prove that a sufficient condition for: (i) A+K to have SVEP on the complement of ${\sigma}_w(A)$ (resp., ${\sigma}_{aw}(A)$) for every compact operator $K{\in}B(\mathcal{X})$ is that ${\sigma}_w(A)$ (resp., ${\sigma}_{aw}(A)$) has no holes; (ii) A + K to be polaroid (resp., left polaroid) for every compact operator $K{\in}B(\mathcal{X})$ is that iso${\sigma}_w(A)$ = ∅ (resp., $iso{\sigma}_{aw}(A)$ = ∅). It is seen that these conditions are also necessary in the case in which the Banach space $\mathcal{X}$ is a Hilbert space.

APPLICATION OF CONVOLUTION SUM ∑k=1N-1σ1(k)σ1(2nN-2nk)

  • Kim, Daeyeoul;Kim, Aeran
    • Journal of applied mathematics & informatics
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    • v.31 no.1_2
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    • pp.45-54
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    • 2013
  • Let $$S^{\pm}_{(n,k)}\;:=\{(a,b,x,y){\in}\mathbb{N}^4:ax+by=n,x{\equiv}{\pm}y\;(mod\;k)\}$$. From the formula $\sum_{(a,b,x,y){\in}S^{\pm}_{(n,k)}}\;ab=4\sum_{^{m{\in}\mathbb{N}}_{m<n/k}}\;{\sigma}_1(m){\sigma}_1(n-km)+\frac{1}{6}{\sigma}_3(n)-\frac{1}{6}{\sigma}_1(n)-{\sigma}_3(\frac{n}{k})+n{\sigma}_1(\frac{n}{k})$, we find the Diophantine solutions for modulo $2^{m^{\prime}}$ and $3^{m^{\prime}}$, where $m^{\prime}{\in}\mathbb{N}$.

Low Power Discrete-Time Incremental Delta Sigma ADC with Passive Integrator (수동형 적분기(Passive Integrator)를 이용한 저전력 이산시간 Incremental Delta Sigma ADC)

  • Oh, Goonseok;Kim, Jintae
    • Journal of the Institute of Electronics and Information Engineers
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    • v.54 no.1
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    • pp.26-32
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    • 2017
  • This paper presents a low power and high resolution incremental delta-sigma ADC that utilizes a passive integrator instead of an opamp-based active integrator. Opamp is a power-hungry block that involves tight design tradeoffs. To avoid the use of active integrator, the s-domain characteristic of an active integrator is first analyzed. Based on the analysis, an active integrator with low gain design is proposed as an alternative design method. To save power even more aggressively, a passive integrator with no static current is proposed. A 1st order single-bit incremental delta-sigma ADC using the proposed passive integrator is implemented in a 65nm CMOS process. Transistor-level simulation shows that the ADC consumes only 0.6uW under 1.2V supply while achieving SNDR of 71dB with 22kHz bandwidth. The estimated total power consumption including digital filter is 6.25uW, and resulting power efficiency is on a par with state-of-the-art A/D converters.

Study on Construction of Modular Cell Line for LCD TV by Lean 6 Sigma (Lean 6 Sigma에 의한 LCD TV의 Modular Cell Line 구축에 관한 연구)

  • Jeong, Young-Kwan;Choi, Seong-Dae;Yoo, Chong-Kyu;Cheong, Seon-Hwan
    • Journal of the Korean Society of Industry Convergence
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    • v.13 no.1
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    • pp.49-54
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    • 2010
  • Lean 6 sigma has recently been used to describe a management system which combines lean management and 6 sigma. The marriage between Lean manufacturing and 6 sigma has proven to be a powerful tool for cutting waste and improving the organization operations. Time and quality are the most important metrics in improving any company's production and profit performance. lean 6 sigma is a management innovation for improving production efficiency, process quality, cost reduction, investment efficiency and customer's satisfaction. in this paper, Advanced cell line is builded the home appliance goods of the LCD TV final assembly line of domestic company line, training the multi-skilled man and controlling the production information system based on Lean 6 sigma.

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