• Journal of Life Science
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• v.24 no.10
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• pp.1046-1054
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• 2014

Beta-1,3-glucanase is widely used in various biotechnological and industrial processes, with over-production required to enable versatile utilization. We examined the overexpression of ${\beta}$-1,3-glucanase (EXGA) from Aspergillus oryzae using ${\delta}$-sequence-mediated integration. We constructed $pRS{\delta}$-exgA and $pRS{\delta}K$-exgA plasmids for integration of the EXGA gene into various chromosomes of Saccharomyces cerevisiae. These plasmids contain the ADH1 promoter for constitutive expression, a signal sequence (exoinulinase signal sequence [INU1 s.s]) for secretory production, and a ${\delta}$-sequence for integration of ${\beta}$-1,3-glucanase. The $pRS{\delta}$-exgA plasmid was transformed into the S. cerevisiae $BY4742{\Delta}exg1$ strain, and ${\beta}$-1.3-glucanase was stably overexpressed and secreted. Another plasmid, $pRS{\delta}K$-exgA, was introduced into the S. cerevisiae $BY4742{\Delta}exg1$ (YKY082) strain, and overexpression of ${\beta}$-1,3-glucanase was examined by inducible integration under geneticin selection. The activity of ${\beta}$-1,3-glucanase increased in accordance with a rise in the geneticin concentration, with 0.8 mg/ml of geneticin suitable for overexpression of ${\beta}$-1,3-glucanase. Subsequently, $pRS{\delta}K$-exgA was repeatedly transformed for sequential ${\delta}$-integration. The activity of ${\beta}$-1,3-glucanase reached about 0.063 unit/ml/$OD_{600}$, 0.095 unit/ml/$OD_{600}$, 0.131 unit/ml/$OD_{600}$ and 0.165 unit/ml/$OD_{600}$ by the first, second, third, and fourth round of integration, respectively. According to the increase in the activity of ${\beta}$-1,3-glucanase by sequential ${\delta}$-integration, the copy number (integration rate) of the EXGA gene also increased in various chromosomes. These results suggest that recombinant ${\beta}$-1,3-glucanase activity can be sequentially increased by repeated ${\delta}$-sequence integration.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.61-64
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• 2001

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double $\delta$ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single $\delta$ system.

• Journal of the Korean Mathematical Society
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• v.38 no.6
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• pp.1191-1204
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• 2001

The paper is devoted to the study of fractional integration and differentiation on a finite interval [a, b] of the real axis in the frame of Hadamard setting. The constructions under consideration generalize the modified integration $\int_{a}^{x}(t/x)^{\mu}f(t)dt/t$ and the modified differentiation ${\delta}+{\mu}({\delta}=xD,D=d/dx)$ with real $\mu$, being taken n times. Conditions are given for such a Hadamard-type fractional integration operator to be bounded in the space $X^{p}_{c}$(a, b) of Lebesgue measurable functions f on $R_{+}=(0,{\infty})$ such that for c${\in}R=(-{\infty}{\infty})$, in particular in the space $L^{p}(0,{\infty})\;(1{\le}{\le}{\infty})$. The existence almost every where is established for the coorresponding Hadamard-type fractional derivative for a function g(x) such that $x^{p}$g(x) have $\delta$ derivatives up to order n-1 on [a, b] and ${\delta}^{n-1}[x^{\mu}$g(x)] is absolutely continuous on [a, b]. Semigroup and reciprocal properties for the above operators are proved.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.826-830
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• 2018

A Cre/loxP-${\delta}$-integration system was developed to allow sequential and simultaneous integration of a multiple gene expression cassette in Saccharomyces cerevisiae. To allow repeated integrations, the reusable Candida glabrata MARKER (CgMARKER) carrying loxP sequences was used, and the integrated CgMARKER was efficiently removed by inducing Cre recombinase. The XYLP and XYLB genes encoding endoxylanase and ${\beta}$-xylosidase, respectively, were used as model genes for xylan metabolism in this system, and the copy number of these genes was increased to 15.8 and 16.9 copies/cell, respectively, by repeated integration. This integration system is a promising approach for the easy construction of yeast strains with enhanced metabolic pathways through multicopy gene expression.

• Journal of the Korean Society of Safety
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• v.11 no.2
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• pp.16-24
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• 1996

In this study we simulate the fatigue test of a compact tension specimen and obtain the displacements, stresses and strains by using the finite element method. And we examine the path independency of $\Delta$J integral values and compare it with $\Delta$J integral values calculated from load-load line displacement curve. From the results of this study, we can find that $\Delta$J integral show the path Independency for saturated materials. We can also find that the path independency of $\Delta$J Is not satisfied when different material Is assumed near the crack tip, but the difference in $\Delta$J is small. And $\Delta$J integral values calculated from load-load line displacement is very analogous with those from integration path but always have lower values than those from integration paths. In the case of crack closing, we found that $\Delta$J integral values from load-load line displacement should be calculated with the load Increment values based on the crack opening point. The unsaturated material is also simulated and its $\Delta$J shows different values according to the path, but the difference is small.

• 한국전산유체공학회:학술대회논문집
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• 2006.10a
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• pp.31-35
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• 2006

The delta-formulation of the Navier-Stokes equations has been popularly used in the aerodynamics area. Implicit algorithm can be easily implemented in that by using Taylor series expansion. This formulation is extended for an unsteady analysis by using a dual-time integration. In the meanwhile, the incompressible flows with heat transfers which occur in the area of thermo-hydraulics have been solved by a segregated algorithm such as the SIMPLE method, where each equation is discretised by using an under-relaxed deferred correction method and solved sequentially. In this study, the dual-time delta formulation is implemented in the segregated Navier-Stokes solver which is based on the collocated cell-centerd scheme with un unstructured mesh FVM. The pressure correction equation is derived by the SIMPLE method. From this study, it was found that the Euler dual-time method in the delta formulation can be combined with the SIMPLE method.

• Journal of the Chungcheong Mathematical Society
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• v.27 no.4
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• pp.689-695
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• 2014

Let M be an N-function satisfies the ${\Delta}_2$-condition and let $O_M$ be the Orlicz space associated with M. Let $C(O_M)$ be the space of all continuous functions defined on the interval [0, T] with values in $O_M$. In this note, we define the analogue of Wiener measure $m^M_{\phi}$ on $C(O_M)$, establish the Wiener integration formulae for the cylinder functions on $C(O_M)$ and give some examples related to our formulae.

• Korean Journal of Optics and Photonics
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• v.16 no.3
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• pp.196-200
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• 2005

A 40 channel arrayed-waveguide grating (AWG) filter operating in C-band and L-band wavelength regions has been fabricated using PLC (Planar Lightwave Circuit) processes with 0.75 refractive index difference. Its design was optimized for matching the center wavelength with the ITU-recommended wavelength. The characteristics of the fabricated C-band AWG are as follows; average insertion loss < 2.5 dB, polarization-dependent loss < 0.3 dB, non-adjacent crosstalk >35dB, and the loss uniformity of 0.8 dB. In the L-band AWG, wavelength accuracy is below 0.02nm.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.9 no.3
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• pp.120-126
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• 1984

In this paper, there is presented the novel encoder circuit design method in the realization of exponential adaption process on the delta modulation coding of speech signals. The digital implementation has been adapted for the illustration of above, especially using a rate multiplier end a double integration circuit. The use of a double integration of the local decoder included in the ADM encoder in prove the undesirable characteristics which the low switching speed of the ratemultiplier couses the SQNR to decreuse, and the SQNR of the decoding signal by above realization is relatively uniformed in wide range of signal levels. The validity of the above design is verified by laboratory experiments.

• The Journal of the Korea Contents Association
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• v.11 no.9
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• pp.1-8
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• 2011

A fuzzy inference technique for real-time textile animation without integration at textile model based Mass-Spring model is introduced. Until now many techniques have used the Mass-Spring model to describe elastically deformable objects like textile. A textile object is able to represent as a deformable surface composed of spring and masses, the movement of textile surface which is analysed through the numerical integration by the fundamental law of dynamics such as Hooke's law. However, the integration methods have 'instability problems' if the explicit Euler's method is applied or 'large amounts of calculation' if the implicit Euler's method is applied. A simple and fast animation technique for Mass-Spring model of a textile with fuzzy inference is proposed. The stabilized simulation result is obtained the state of each mass-point in real-time for the n of mass-points by a relatively simple calculation.