Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Simultaneous and Sequential Integration by Cre/loxP Site-Specific Recombination in Saccharomyces cerevisiae

  • Choi, Ho-Jung (Biomedical Engineering and Biotechnology Major, Divison of Applied Bioengineering, Dong-Eui University) ;
  • Kim, Yeon-Hee (Biomedical Engineering and Biotechnology Major, Divison of Applied Bioengineering, Dong-Eui University)
  • Received : 2018.02.01
  • Accepted : 2018.02.22
  • Published : 2018.05.28
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Abstract

A Cre/loxP-${\delta}$-integration system was developed to allow sequential and simultaneous integration of a multiple gene expression cassette in Saccharomyces cerevisiae. To allow repeated integrations, the reusable Candida glabrata MARKER (CgMARKER) carrying loxP sequences was used, and the integrated CgMARKER was efficiently removed by inducing Cre recombinase. The XYLP and XYLB genes encoding endoxylanase and ${\beta}$-xylosidase, respectively, were used as model genes for xylan metabolism in this system, and the copy number of these genes was increased to 15.8 and 16.9 copies/cell, respectively, by repeated integration. This integration system is a promising approach for the easy construction of yeast strains with enhanced metabolic pathways through multicopy gene expression.

Keywords

References

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