Objectives: The purpose of this study was to examine the association between participation in physical activities (PA) and subjective well-being. Methods: We analyzed the data from Korean Youth Risk Behavior Web-based Survey, 2013 containing 36,655 and 35,780 for male and female students respectively. Study variables included PA, participation in physical education class, participation in team sports and subjective well-being. Control variables were school type, socio-economic status, level of parent education, living with parent, academic performance, delinquent behaviors, depression and perceived health. For this study, descriptive analysis, Chi-square test, and multivariate logistic regression analysis were performed. Results: Among boys, after adjusting for control variables, PA (past 7 days 0 time reference group vs. over 4 times: OR 1.306), physical education class (past 7 days 0 time reference group vs. over 3 times: OR 1.140), and team sports (no team reference group vs. 2 team: OR 1.206) were associated with subjective well-being. Among girls, control variables were adjusted. PA (past 7 days 0 time reference group vs over 4 times: OR 1.244), physical education class (past 7 days 0 time reference group vs. over 3 times: OR 1.161), and team sports (no team reference group vs. 2 team: OR 1.181) were associated with subjective well-being. Conclusions: Participation in physical activities may help adolescent promote their life satisfaction. Thus, education experts should develop various exercise curriculums to encourage participation in physical activities.