Suppose that n is a positive integer. For any real number $\alpha$($\beta$ resp.) with $\alpha$ < 1 ($\beta$ > 1 resp.), let $K^{(n)}(\alpha)$ ($K^{(n)}(\beta)$ resp.) be the class of analytic functions in the unit disk $\mathbb{D}$ with f(0) = f'(0) = $\cdots$ = $f^{(n-1)}(0)$ = $f^{(n)}(0)-1\;=\;0$, Re($\frac{zf^{n+1}(z)}{f^{(n)}(z)}+1$) > $\alpha$ (Re($\frac{zf^{n+1}(z)}{f^{(n)}(z)}+1$) < $\beta$ resp.) in $\mathbb{D}$, and for any ${\lambda}\;{\in}\;\bar{\mathbb{D}}$, let $K^{(n)}({\alpha},\;{\lambda})$ $K^{(n)}({\beta},\;{\lambda})$ resp.) denote a subclass of $K^{(n)}(\alpha)$ ($K^{(n)}(\beta)$ resp.) whose elements satisfy some condition about derivatives. For any fixed $z_0\;{\in}\;\mathbb{D}$, we shall determine the two regions of variability $V^{(n)}(z_0,\;{\alpha})$, ($V^{(n)}(z_0,\;{\beta})$ resp.) and $V^{(n)}(z_0,\;{\alpha},\;{\lambda})$ ($V^{(n)}(z_0,\;{\beta},\;{\lambda})$ resp.). Also we shall determine the extreme points of the families of analytic functions which satisfy $f(\mathbb{D})\;{\subset}\;V^{(n)}(z_0,\;{\alpha})$ ($f(\mathbb{D})\;{\subset}\;V^{(n)}(z_0,\;{\beta})$ resp.) when f ranges over the classes $K^{(n)}(\alpha)$ ($K^{(n)(\beta)$ resp.) and $K^{(n)}({\alpha},\;{\lambda})$ ($K^{(n)}({\beta},\;{\lambda})$ resp.), respectively.