대한한의학방제학회지 (Herbal Formula Science)

  • 제32권1호
  • /
  • Pages.83-90
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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새싹인삼 추출물의 항산화 활성 및 MMP-1 저해 활성

Antioxidant activity and MMP-1 inhibitory activity of Panax Ginseng Sprout Extracts

  • 김민정 (경상국립대학교 수의과대학) ;
  • 양예진 (경상국립대학교 수의과대학) ;
  • 양주혜 (한국한의학연구원 한의기술응용센터) ;
  • 이원융 (원광대학교 한의과대학) ;
  • 김우현 (경상국립대학교 수의과대학) ;
  • 이재남 (건국대학교 산업대학원 향장학과) ;
  • 박광일 (경상국립대학교 수의과대학)
  • Min-Jung Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Ye-Jin Yang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Ju-Hye Yang (Korean Institute of Oriental Medicine Technology Application Center) ;
  • Won-Yung Lee (College of Oriental Medicine, Wonkwang University) ;
  • Woo-Hyun Kim (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jae-Nam Lee (Graduate School of Engineering Konkuk University) ;
  • Kwang-Il Park (College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2024.01.25
  • 심사 : 2024.02.16
  • 발행 : 2024.02.28
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초록

Objectives : As a substitute for high-price ginseng, this study attempted to examine a possibility of the ferment extract of Panax ginseng sprout whether leaves and roots can be used together as a cosmetic ingredient with anti-oxidative and wrinkle-care effects. Methods : In terms of a test method, antioxidant activities were confirmed through total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity and ABTS radical scavenging activity using the Panax ginseng sprout. In addition, to assess wrinkle-care effectiveness, the cytotoxicity of the extract was analyzed through MTT assay, and inhibition of collagenase activity in the cells was tested using the Panax ginseng sprout fermented by Saccharomyces cerevisiae. Resuits : The content of polyphenols and flavonoids in natural plants was highest in Panax Ginseng Sprout Extract at 100℃, which also demonstrated high DPPH, ABTS radical scavenging activity. MTT assay demonstrated that the Panax Ginseng Sprout Ferment Extract did not have a cytotoxic effect in CCD-986SK cell. Also, Panax Ginseng Sprout Ferment Extract was found to inhibit MMP-1 expression by 51.85±6.09% at a concentration of 10%. Conclusions : Therefore, this study has confirmed a possibility of Panax ginseng sprout ferment extract as a cosmetic ingredient with MMP-1-inhibitory effects.

키워드

과제정보

본 연구는 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 3단계 산학협력 선도대학 육성사업(LINK 3.0) (No.202306390001) 및 지역대학우수과학자지원사업(No. 2022R1I1A3053818)의 지원을 받아 수행되었습니다.

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