대한한의학방제학회지 (Herbal Formula Science)

  • 제32권1호
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  • Pages.63-82
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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산화적 스트레스에 대한 생간건비탕가음양곽(生肝健脾湯加淫羊藿) (2:1)의 간보호효과

SKT + EKE (2:1) protects oxidative stress induced-liver damage

  • 박상미 (대구한의대학교 한의과대학) ;
  • 정대화 (대구한의대학교 제약공학과) ;
  • 진효정 (대구한의대학교 한의과대학) ;
  • 김예림 (대구한의대학교 한의과대학) ;
  • 김경순 (대구한의대학교 한의과대학) ;
  • 황보민 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Dae Hwa Jung (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Hyo Jeong Jin (College of Korean Medicine, Daegu Haany University) ;
  • Ye Lim Kim (College of Korean Medicine, Daegu Haany University) ;
  • Kyung-soon Kim (College of Korean Medicine, Daegu Haany University) ;
  • Min Hwangbo (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 투고 : 2024.01.18
  • 심사 : 2024.02.15
  • 발행 : 2024.02.28
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초록

Objective : Saengkankunbi-tang (SKT) is used as a traditional Korean herbal formula for treatment of liver diseases. We investigated the hepatoprotective effects of SKT plus Epimedium koreanum Nakai (EKE) against arachidonic acid (AA) + iron-mediated cytotoxicity in HepG2 cells and carbon tetrachloride (CCl4)-mediated acute liver damage in mice. Methods : The cyto-protective effects of SKT + EKE were determined by MTT assay, western blot and fluorescence activated cell sorting analysis. In mice, blood biochemistry and western blot were assessed in CCl4-induced acute hepatic damage. The animal groups included vehicle-treated control, CCl4, SKT (200 mg/kg/day), EKE (100 mg/kg/day), SKT (200 mg/kg/day) + EKE (100 mg/kg/day) and silymarin (200 mg/kg/day). Results : In HepG2 cells, pretreatment with SKT + EKE significantly suppressed cytotoxicity induced by AA + iron and reduced the expression of proteins related to apoptosis. In addition, pretreatment with SKT + EKE significantly prevented the increase in H2O2 production, GSH depletion, and lower mitochondrial membrane potential induced by AA + iron. In CCl4-induced liver damage mice, the administration of SKT + EKE prevented the liver damage by inhibition of hepatocyte damage and expression of apoptosis proteins in liver. More importantly, in vitro and in vivo assay, SKT + EKE showed significant effect compare with SKT alone or EKE alone in all parameters. Conclusions : These results indicated that SKT + EKE could protect against oxidative stress-induced liver damage, and SKT + EKE is more effective than SKT alone or EKE alone.

키워드

과제정보

This study was supported by the National Research Foundation of Korea funded by Korea government (MSIP) (Grant No.2018R1A5A2025272)

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