대한한의학방제학회지 (Herbal Formula Science)

  • 제32권1호
  • /
  • Pages.51-61
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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연교의 항산화 효과 연구

Anti-oxidant effect of forsythia suspensa on cellular damage in the chronic disease

  • 김영은 (동국대학교 한의과대학 방제학교실) ;
  • 김민진 (동국대학교 한의과대학 방제학교실) ;
  • 배수진 (동국대학교 한의과대학 방제학교실) ;
  • 박선빈 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실) ;
  • 박광일 (경상대학교 수의과대학 생리학 실험실) ;
  • 김영우 (동국대학교 한의과대학 방제학교실)
  • Young-Eun Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Min-Jin Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Su-Jin Bae (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Seon Been Bak (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Sun-Dong Park (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Kwang-Il Park (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Young Woo Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University)
  • 투고 : 2024.01.31
  • 심사 : 2024.02.13
  • 발행 : 2024.02.28
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초록

Objectives : This study induced oxidative stress in HepG2 cells by treating them with AA+iron and investigated the effects of forsythia suspensa extract on this stress, as well as elucidated the molecular mechanisms underlying its hepatoprotective effects. Methods : To confirm the antioxidative effects of FSE, HepG2 cells were induced with AA+iron to induce oxidative stress, followed by MTT assay. Additionally, the effect of FSE in reducing the increased ROS levels and mitochondrial damage induced by AA+iron in HepG2 cells was confirmed using FACS. Furthermore, western blot analysis were conducted to investigate the molecular mechanisms underlying the hepatoprotective effects of FSE. Results : FSE increased the decreased cell viability induced by AA+iron. Additionally, FSE normalized the expression of apoptosis-related proteins induced by AA+iron. The elevated ROS levels in HepG2 cells induced by AA+iron were reduced by FSE, and the increase in Rh123-negative cells induced by AA+iron was attenuated by FSE. Moreover, FSE activated the protein expression of AMPK and its related phosphorylating enzymes, LKB1 and ACC. Furthermore, FSE activated YAP and its upstream phosphorylating enzyme, LATS1. Conclusions : These results demonstrate that FSE has an inhibitory effect on oxidative stress induced by AA+iron and may have potential hepatoprotective effects.

키워드

과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (number: HF20C0212). This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government [MSIT] (RS-2023-00218419) (NRF-2022R1I1A3053818) (2022R1A2C1092168). 그리고, 본 연구는 교육부와 한국연구재단의 재원으로 지원을 받아 수행된 3단계 산학협력 선도대학 육성사업(LINK 3.0) (No.202306390001)에 의하여 연구되었다.

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