• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.102-107
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• 2001

This study was carried out to explore for the possibility of recycling the pine wood sawdusts for the substrate for enokitake (Flammulina velutipes) cultivation. The wood species of sawdusts cultivated for enokitake mushroom were identified mostly as hard pine (Pinus spp.). Distribution of enokitake hyphae was restricted to ray parenchymas and tracheids exposed to fungi. Nevertheless, degree of cell wall degradation by enokitake was slight. Light microscopic observation showed the thinning of secondary cell wall in some tracheids. Under polarized microscopy the 1()ss of birefringence was observed only in a few latewood tracheids. All the middle lamella remained intact. The present work showed clearly that pine sawdusts used as substrate for enokitake cultivation held enough cell wall materials for mushroom cultivation. The relative resistance of softwood cell walls against enokitake fungus was also discussed.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.70-75
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• 2007

We conducted an inoculation test using nine open-pollinated families of pine trees to evaluate their susceptibility and mortality in different densities of pine wood nematode. Three-year-old nine open-pollinated pine families were inoculated with Bursaphelenchus xylophilus at levels of 3,000, 5,000, and 7,000 nematodes/seedling in greenhouse. There were no distinct patterns in latent period among three densities of B. xylophilus in all families. Most families showed the first disease symptoms of needle discoloration within 12-15 days after inoculation. However, open-pollinated progenies of Pinus densiflora showed the longest latent period because none of one-year-old needles were wilted until 14 days after inoculation with 5,000 and 7,000 nematodes. One-year-old needles were wilted earlier than current needles in all tested families with all densities of B. xylophilus. Current needles were not wilted until 14 days after inoculation in all seedlings. The mortality of all seedlings rapidly increased from 35 days to 49 days after inoculation, and all died within 80 days except two seedlings. A 3,000 nematodes/100 ${\mu}L$ with sterilized distilled water are enough to screen 3-year-old pine seedlings for resistance to B. xylophilus.

• Journal of the Korean Wood Science and Technology
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• v.10 no.3
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• pp.152-164
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• 1982

1. In order to make the improvement of plywood quality, this study has been made. The pretreatments applied to the veneers are as follows. a) The soaking treatment of the veneer in 30 percent methanol solution for 24 hours was applied to remove some resinous materials which may cause many stain discolouring on the face of finished plywood. b) The preservative treatment of the veneer in 2 percent malenit solution for 2 to 3 minutes was adopted to make resistance against decay and insect damages. c) The fire retardant treatment of the veneer in 40 percent ammonium phosphate solution for 2 hours was applied to give retardation effect against fire burning. 2. The results summarized in this study are as follows. a) One percent resinous materials was extracted, after the soaking of the veneers in 30 percent diluted methanol. b) No marks of the dirty stains of resinous materials on the face of the treated plywood was shown, although many quite dirty stains on the face of untreated plywood have contrary seen. c) However, the strip shear test strength of the treated plywood was not decreased. It means that there is no difference in the strength between the treated plywood and the untreated plywood. The strength values were 25.08 kg/$cm^2$ and 24.98 kg/$cm^2$, respectively. d) The strip shear test strength of plywood made of the treated veneers in 2 percent malenit solution was not decreased. e) The slight decrease of the strip shear test strength of the treated plywood made of the treated veneer in 40 percent ammonuim phosphate solution was shown. However, the remarkable difference of the fire retardation activities between the treated specimens and the untreated specimens has seen as in Table 10, that is, the fire proofed specimens had taken about 28 seconds to start to burn, while the untreated specimens had taken 15 seconds to reach to burning. This means that the fire retardation effect of the fire proofed plywood was greater than that of the unproofed plywood.

• Journal of the Korean Wood Science and Technology
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• v.14 no.2
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• pp.29-35
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• 1986

This study was carried out to examine the effect of aluminium sulfate addition to the alkali-acid catalyst phenolic resin for the manufacture of the kapur (Dryobalanops spp.) plywood on the reduction of phenolic resin spreading. On the manufacture of plywood, Adhesive Contents such as 50g/$m^2$, 75g/$m^2$ and 150g/$m^2$ were treated. The spreading adhesive content of 50g/$m^2$ and 75g/$m^2$ had been controlled to about 150g/$m^2$ added with the water in order to get sufficient spreading and controlled to pH 4.5 with aluminium sulfate [$Al_2(SO_4)_3$]. The results are summarized as follows: 1. Specific gravities of air dried plywood manufactured from each adhesive ranged from 0.77 to 0.86 and their moisture contents met the KS requirements. 2. In dry and wet shear strengths, 150 $P_{Al{\cdot}Ac}$ adhesive showed the highest and 75 $P_{Al{\cdot}Ac{\cdot}Am}$ adhesive indicated higher value than 150 $P_{Al{\cdot}Ac}$ adhesive. 3. In case of glue shear strength after boiling test, 150 $P_{Al{\cdot}Ac}$ adhesive was the best and adding of aluminium sulfate was not effective on reinforcement of boiling water resistance of phenolic resin, but met KS requirements. 4. 75 $P_{Al{\cdot}Ac{\cdot}Am}$ adhesive showed the good shear strength and met KS requirements. Therefore, adding of aluminium sulfate was very efficient for economical plywood manufacture.

• Journal of Korea Foresty Energy
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• v.25 no.2
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• pp.28-33
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• 2006

This study was conducted to find potentialities of the leaves of gingko tree (Gingko biloba L.) which has been planted as a roadside tree in Korea because of its resistance on air pollution, insect, fungi, etc. Various amounts of the leaves were mixed with wasted wood particles to manufacture particleboard. Their influences on physical and mechanical properties and the formaldehyde emission of PB were investigated. Physical and mechanical properties, such as density, modulus of rupture (MOR), and internal bond (IB) strength, of manufactured particleboard were not much different from those of control board. Formaldehyde emission values were decreased with increasing amount of leaves in PB prepared. Especially, particleboard made with 3 percent of leaves was decreased to $1.66mg/{\ell}$ in formaldehyde emission, which is about 40% lower emission than that of control. From these results, the leaves of gingko tree may be considered as a formaldehyde emission lowering additive in a functional PB manufacturing process.

• Journal of the Korean Wood Science and Technology
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• v.20 no.2
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• pp.9-22
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• 1992

The aim of this research was to investigate physical and mechanical properties of various composition panels, each fabricated with a ratio of fiber to particle of 2 to 10. Type A consisted of fiber-faces and particle-core in layered-mat system. Type B consisted of fiberboard-faces on particleboard-core. Type C consisted of fibers and particles in mixed-mat system. The results obtained from tests of bending strength, internal bond, screw holding strength and stability were as follows: 1. The bending strength and internal bonding of both the Type A panel and the Type B panel were higher than those of the Type C panel and three-layered particle board. 2. The mechanical properties of the Type C panel showed the lowest values of all composition methods. It seems that the different compression ratios of the particle and fiber interrupted the densification of the fibers when hot pressed. 3. The dimensional stability of layered-mat system panels consising of fiber-faces and particle-core was better the than control particleboard. 4. In composition methods of particle and fiber, layered-composition method was more resonable than mixed-composition. The Type B panel had the highest mechanical properties of all the composition types. 5. The Type A panel was considered the ideal composition method because of its resistance to delamination between the particle-layer and the fiber-layer and because of its lower adhesive content and more effective manufa cturing process.

• Journal of Microbiology
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• v.44 no.2
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• pp.177-184
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• 2006

The characteristic biodegradation of monomeric styrene by Phanerochaete chrysosporium KFRI 20742, Trametes versicolor KFRI 20251 and Daldinia concentrica KFRI 40-1 was carried out to examine the resistance, its degradation efficiency and metabolites analysis. The estrogenic reduction effect of styrene by the fungi was also evaluated. The mycelium growth of fungi differentiated depending on the concentration levels of styrene. Additionally P. chrysosporium KFRI 20742 showed superior mycelium growth at less than 200 mg/l, while D. concentrica KFRI 40-1 was more than 200 mg/l. The degradation efficiency reached 99 % during one day of incubation for all the fungi. Both manganese-dependent peroxidase and laccase activities in liquid medium were the highest at the initial stage of incubation, whereas the lowest was after the addition of styrene. However, both activities were gradually recovered after. The major metabolites of styrene by P. chrysosporium KFRI 20742 were 2-phenyl ethanol, benzoic acid, cyclohexadiene-1,4-dione, butanol and succinic acid. From one to seven days of incubating the fungi, the expression of pS2 mRNA widely known as an estrogen response gene was decreased down to the level of baseline after one day. Also, the estrogenic effect of styrene completely disappeared after treatment with supernatant of P. chrysosporium KFRI 20742 from one week of culture down to the levels of vehicle.

• Korean Journal of Microbiology
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• v.50 no.4
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• pp.372-375
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• 2014

This experiment was undertaken to investigate proper conditions for protoplast isolation and genetic transformation of the white rot fungi, Polyporus brumalis. The protoplasts were formed from mycelia at a frequency of $1{\times}10^7/ml$ with 0.5% Usukizyme. The transformation vector (pHYgpt) was constructed using hygromycin resistance gene (hph) for the selectable maker. The yield was 100-160 transformants/${\mu}g$ DNA in a transformation mediated by 40% polyethylene glycol solution with aurintricarboxylic acid, heparin and supermidine. The genomic integration of the pHYgpt was confirmed by hph-specific PCR and the expected amplified band appeared only in the transformants. These results could be an efficient tool in gene engineering of the genus polyporus.

• Journal of the Korean Wood Science and Technology
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• v.25 no.4
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• pp.17-21
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• 1997

This study was carried out to develop the manufacturing technique of Korean paper(Hanji) and find out new uses of machine-made Hanji produced on a a large scale by paper machine. Six kinds of Hanji were made and laminated with backpaper by acryl-based or vinyl acetate ethylene-based adhesive. Six kinds of wallpapers were made such as WH 1, WH 2-1, WH 2-2, WH 3-1, WH 3-2 and WH 4. Physical properties and quality test on the wallpapers were examined. Physical strength of the wallpapers was better than each strength of the Hanji and backpaper. It might be a roll of adhesive. And so, Production cost of the Hanji could be brought down if the Hanji was made thin to get strength not enough to cut during lamination of two papers(Hanji and backpaper). Hanji wallpapers of WH 3-1 and WH 4 are lower than a standard in sunlight resistance and WH 1 is in properties of opacity. WH 2-1, WH 2-2 and WH 3-2 were better than Korean standard in some quality tests. WH 2-2 and WH 3-2 were superior to other wallpapers as a whole. About, twenty kinds of wallpapers with surface treatment, are now being produced on the basis of these two types of wallpapers.

• Journal of the Korean Wood Science and Technology
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• v.32 no.1
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• pp.80-87
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• 2004

This study was carried out to improve the properties of powdered tannin adhesive(PT) by adding liquid tannin resin(LT) to PT in the manufacture of particleboard. Mixing the LT to PT from 50% to 100% by weight did not show any difference in particleboard properties, but the higher the powdered tannin resin ratio, the lower the properties of the board. The proper ratio of PT to LT was 30:70 for the improvement of PT-particleboard, unless LT lower than 70%. Internal bonding strength was in proportional to the amount of LT. Mixing amino adhesives and PT did not show any improvements in mechanical and physical properties of the board but they only acted as scavenger for the free formaldehyde.Manufacturing particle board with the adhesive of 30:70 (PT:LT) and by using double blender resulted in high-performance products of E₀ level of formaldehyde emission with high water resistance (U type; below 12%, M type; below 25%), as well as saving chip drying energy.