The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Variation in Susceptibility of Pine Species Seedlings with the Pine Wood Nematode, Bursaphelenchus xylophilus, in Greenhouse

  • Woo, Kwan-Soo (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Yeong-Sik (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Koo, Yeong-Bon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Yeo, Jin-Kie (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Moon, Yil-Soong (Southern Forest Research Center, Korea Forest Research Institute)
  • Published : 2007.06.30
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Abstract

We conducted an inoculation test using nine open-pollinated families of pine trees to evaluate their susceptibility and mortality in different densities of pine wood nematode. Three-year-old nine open-pollinated pine families were inoculated with Bursaphelenchus xylophilus at levels of 3,000, 5,000, and 7,000 nematodes/seedling in greenhouse. There were no distinct patterns in latent period among three densities of B. xylophilus in all families. Most families showed the first disease symptoms of needle discoloration within 12-15 days after inoculation. However, open-pollinated progenies of Pinus densiflora showed the longest latent period because none of one-year-old needles were wilted until 14 days after inoculation with 5,000 and 7,000 nematodes. One-year-old needles were wilted earlier than current needles in all tested families with all densities of B. xylophilus. Current needles were not wilted until 14 days after inoculation in all seedlings. The mortality of all seedlings rapidly increased from 35 days to 49 days after inoculation, and all died within 80 days except two seedlings. A 3,000 nematodes/100 ${\mu}L$ with sterilized distilled water are enough to screen 3-year-old pine seedlings for resistance to B. xylophilus.

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References

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