• 한국발생생물학회지:발생과생식
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• 제6권1호
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• pp.25-30
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• 2002

내분비계 장애물질은 다양한 동물의 생식과 배아의 발생에 위해한 영향을 미친다고 보고되었다. 이러한 초기 배아의 비정상 발생 중 대표적인 것이 할구파편이다. 본 연구에서는 착상 전 생쥐 초기 배아를 PCB, BPA, DDT에 체내, 체외에서 각각 노출시켰다. 내분비계 장애 물질에 노출시켰던 배아는 할구파편, 불규칙한 할구와 깨짐, 일부 파괴된 투명대 배아 등의 다양한 형태학적 비정상 양상을 보였다. 착상 전 생쥐 초기배아의 세포괴사에 관여하는 유전자를 조사하기 위하여 RT-PCR을 사용하여 mRNA수준에서 평가하였다. Bcl-2, bad 그리고 bax유전자 발현 정도를 대조군과 내분비계 장애물질 처리군으로 나누어 비교하였다. Bcl-2유전자는 내분비계 장애물질 처리군에서 낮아지는 경향을 보였고, bad와bix는 내분비계 장애물질 처리군에서 보다 높은 경향을 보였다. 이러한 연구 결과는 착상 전 생쥐 초기배아를 체내와 체외에서 내분비계 장애물질에 노출시킴으로써 발생적 독성물질을 신속히 판단할수 있는 유용한 수단이 될 수 있을 것으로 생각한다.

• 한국환경생태학회지
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• 제24권1호
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• pp.46-53
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• 2010

본 연구의 목적은 오대산국립공원 내 도로를 대상으로 2003년에서 2007년까지 로드킬 현황을 조사하고 종별 발생특성, 월별 발생분포, 토지이용 현황별 로드킬 특성을 분석하여, 로드킬 저감을 위한 기초자료를 제시하는 것이었다. 로드킬은 총 427건이 발생하였으며, 대부분 소형포유류인 것으로 나타났다. 포유류와 파충류에서는 다람쥐와 누룩뱀, 조류는 노랑턱멧새의 발생빈도가 높았다. 포유류와 파충류는 여름철과 가을철, 조류는 봄부터 여름사이의 발생빈도가 높게 나타났으며, 로드킬은 야생통물의 활통시기 및 번식시기 그리고 차량 이통량 증가와 밀접한 관련이 있는 것으로 나타났다. 야생동물 로드킬 빈도는 도로변 토지이용형태에 의해 영향을 받는 것으로 나타났으며, 도로 양측이 밭인 경우에 가장 높았다.

• Journal of Ginseng Research
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• 제40권4호
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• pp.382-394
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• 2016

Background: Ginsenosides are the characteristic and principal components which manifest a variety of the biological and pharmacological activities of the roots and rhizomes of Panax ginseng (GRR). This study was carried out to qualitatively and quantitatively determine the ginsenosides in the cultivated and forest GRR. Methods: A rapid and sensitive ultra-high-performance liquid chromatography coupled with diode-array detector and quadrupole/time of flight tandem mass spectrometry (UPLC-DAD-QTOF-MS/MS) was applied to the qualitative analysis of ginsenosides and a 4000 QTRAP triple quadrupole tandem mass spectrometer (HPLC-ESI-MS) was applied to quantitative analysis of 19 ginsenosides. Results: In the qualitative analysis, all ingredients were separated in 10 min. A total of 131 ginsenosides were detected in cultivated and forest GRR. The method for the quantitative determination was validated for linearity, precision, and limits of detection and quantification. 19 representative ginsenosides were quantitated. The total content of all 19 ginsenosides in the forest GRR were much higher than those in the cultivated GRR, and were increased with the growing ages. Conclusion: This newly developed analysis method could be applied to the quality assessment of GRR as well as the distinction between cultivated and forest GRR.

• 한국임상수의학회지
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• 제23권1호
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• pp.81-85
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• 2006

Complete ruptures of the Achilles tendon were repaired in two hunting dogs of a 3-year-old, 20 kg and a 4-year-old, 14 kg with non weight bearing lameness. Both dogs were injured by wild animals and the skin defect was showed on the caudal surface of the right limb. Surgical repair involved the attachment of the ruptured tendon with a three-loop pulley method, using nonabsorbable suture materials. After surgical procedure, a bivalved cast was applied and maintained for 6 weeks. Exercise was restricted for 2 weeks more before gradually returned to normal. Gait and standing posture of dogs were returned to normal during the follow-up of 10 weeks.

• 식물분류학회지
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• 제47권1호
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• pp.11-22
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• 2017

Prangos fedtschenkoi (Regel et Schmalh.) Korovin (Apiaceae) is an endemic species for mountainous Middle Asia, which is both a rare and useful plant. Organic extractions from this species are being used in pharmaceutics and cosmetology. In recent years, P. fedtschenkoi distribution area has considerably decreased, presumably, resulting from human activities such as agriculture, construction works, overgrazing and collection from wild for pharmaceutic purposes. Six populations were found in Uzbekistan and their genetic divergence and differentiation were studied with 10 inter-simple sequence repeat (ISSR) markers, selected out of 101. Totally 166 amplified ISSR fragments (loci) were revealed, of which 164 were polymorphic. Relatively moderate level of polymorphism was found at population level with polymorphic bands ranging from 27.71% to 47.59%. Mean P = 39.05%, $N_a=1.40$, $N_e=1.25$, S.I. = 0.21, and $H_e=0.14$ were revealed for all loci across six populations. AMOVA showed higher variation among populations (62%) than within them (38%). The Bayesian model determined 5 clusters, or genetic groups. The posteriori distribution of the Theta II estimator detected full model identifying high inbreeding, intensified by low gene flow (Nm = 0.3954). Mantel test confined population 6 as distinct cluster corresponding to geographic remoteness (R = 0.5137, $p{\leq}0.005$). Results were used as the bases for developing conserve measures to restore populations.

• 한국환경생태학회지
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• 제7권2호
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• pp.207-212
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• 1994

덕유산 국립 공원의 양서류강을 1993년 4월 10일, 1993년 8월 8일부터 9일 두차례에 걸쳐 무주 리조트 골프장건설 지역과 지봉 지역에서 조사하였다. 골프장 건설 지역에서는 도롱뇽, 무당개구리, 두꺼비, 참개구리 및 북방산개구리가 관찰되었고 지봉에서는 꼬리치 레도롱뇽과 북방산개구리가 관찰되어 덕유산 국립 공원 지역에서 발견된 양서류는 환경처가 지정한 특정야생동물 꼬리치레도롱뇽, 도롱뇽, 및 두꺼비 3종을 포함하여 총 2목 4과 5속 6종이었다. 비교적 많은 9개체의 꼬리치레도롱뇽이 관찰된 지봉은 해발고도가 높고 수온이 낮으며 자연환경의 보존상태가 양호하고 인적이 드물어 꼬리치레도롱뇽 서식에 적합한 지역으로 사료되어 그 보호가 요망된다. 다수의 도롱뇽 유생과 2개체의 두꺼비를 포함하여 5종의 양서류가 관찰된 골프장 건설 지역은 공사가 진행됨에 따른 종과 개체수의 격감현상을 볼 수 있었다. 국립공원과 같은 자연환경 지역에서의 대형공사는 생물종 다양성 보존에 크게 역행하는 것으로 자제되어야 할 것이다.

• 자원ㆍ환경경제연구
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• 제28권4호
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• pp.617-643
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• 2019

DMZ는 1953년 정전협정 이후 설정된 군사분계선의 남북으로 설정된 완충지대이다. 또한 정전협정 이후 철저히 사람의 간섭으로부터 단절된 지역이고, 67종의 멸종위기종을 포함한 2,716종의 야생동·식물이 서식하는 중요한 자연환경보전지역이다. 반면, 향후 남북경협 활성화 시 DMZ에 대한 개발압력은 증가될 수밖에 없기에, DMZ 개발 시 경제적 비용-편익 관점에서의 논의뿐 아니라, DMZ의 생물다양성을 어떻게 평가하고 보존할 것인지와 복구비용 및 예산도 종합적으로 고려해야 한다. 본 논문은 실물옵션 방법론으로 생물다양성의 가치를 고려한 DMZ 관리 모형을 제시하였다. 또한 DMZ의 정치적 리스크 및 지역적 특수성을 모형 안에 반영하여 설계하였다. 실증분석을 통해 불확실성하에서 DMZ 투자경제성을 확보할 수 있는 생물다양성 가치의 임계값(b^*)을 도출하였으며, 이때 생물다양성이 주는 편익을 도출하였다. 그리고 민감도 분석을 통해 생물다양성 가치의 임계값에 영향을 주는 요인을 분석하였으며, 이를 바탕으로 DMZ의 지속가능한 개발을 위한 정책적 시사점을 제시하였다.

• 환경영향평가
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• 제20권3호
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• pp.367-379
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• 2011

This study was conducted to develop an ecological park planning model to enhance the functions of habitats and ecological corridors in Green Belt Areas, because changing policies have resulted in the degredation of the Green Belts due to progressive fragmentation of ecosystems. The principal outcome of the study is to plan an ecological park model through the restoration of habitats. In order to evaluate the capacity of the model to enhance the ecological functions of habitats and ecological corridors in Green Belt Areas, a simulation of habitats was carried out in the Sungnam-Yusoo region. The model was developed via following steps: 1. Selection of candidate sites and selection of the study site by analyzing development factors; 2. Selection of target species that can represent the habitat at the site; 3. Analysis of the site's suitability index for the target species; 4. Establishment of a conceptual plan to enhance and expand the currently produced suitability index; 5. Creation of a master plan based on the conceptual plan; and 6. Evaluation of the enhanced and expanded suitability index of the site. The study showed that the Habitat Unit (HU) of Rana coreana, which was selected as the target species of the study, increased from $28,044m^2$(3.6%) to $224,352m^2$(28.8%), and the HU of the site as the ecological corridor for wild animals increased from $4,674m^2$(0.6%) to $152,684m^2$(19.6%). The study results show that the ecological deficits of the Green Belt Area can be overcome by enhancing the ecological functions of the region, which should be beneficial. The model could be utilized for effective enhancement and management of other Green Belt Areas.

• 한국환경복원기술학회지
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• 제12권3호
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• pp.49-58
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• 2009

This study was done to provide a case model with a concept of environmental integration based on the water circulation system. Area of interest (AOI) is the Eastern Pangyo New Town area, which has several advantages in adaptation of a water circulation system. The AOI has a potential of maintaining water resources, and has a good condition to construct the water circulation system. Research done for the purpose of the establishment of the water circulation system in the Eastern Pangyo New Town shows the following. The main sources of water supply in the water circulation system in the Eastern Pangyo New Town is from two subway stations and runoff water, along with the natural water flowing from the mountains, rain water, and stream water. It was determined that more than 35,000 tons of water would be needed for the creation of water circulation system at the Eastern Pangyo. If the creation of infrastructure for the use of runoff and rain water as well as the periodic management can be provided, it can serve as the new model for a new city with water circulation system. In addition, since the Eastern Pangyo New Town water circulation system can secure enough amount of water resources, natural drainage system (NDS) in which it can be in dry condition in non-rainy days, is applied and connected to the typical waterways. Such water circulation system has many positive aspects including the wise use of water resources, and providing wild Life animals corridors and habitats. Also, the water circulation system can lead to the environmental education to the residents and visitors on environmental awareness of the water circulation system and their environment.

• Molecules and Cells
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• 제37권1호
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• pp.24-30
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• 2014

Inductive expression of early growth response 1 (Egr-1) in neurons is associated with many forms of neuronal activity. However, only a few Egr-1 target genes are known in the brain. The results of this study demonstrate that Egr-1 knockout (KO) mice display impaired contextual extinction learning and normal fear acquisition relative to wild-type (WT) control animals. Genome-wide microarray experiments revealed 368 differentially expressed genes in the hippocampus of Egr-1 WT exposed to different phases of a fear conditioning paradigm compared to gene expression profiles in the hippocampus of KO mice. Some of genes, such as serotonin receptor 2C (Htr2c), neuropeptide B (Npb), neuronal PAS domain protein 4 (Npas4), NPY receptor Y1 (Npy1r), fatty acid binding protein 7 (Fabp7), and neuropeptide Y (Npy) are known to regulate processing of fearful memories, and promoter analyses demonstrated that several of these genes contained Egr-1 binding sites. This study provides a useful list of potential Egr-1 target genes which may be regulated during fear memory processing.