• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.102-102
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• 2018

Protaetia Brevitarsis Seulensis(white grub) has been traditionally used as medicinal stuff to treat blood stasis, occlusion of menstruation, tetanus and liver cancer in Asian countries (Korea, Japan, China, Taiwan, India and Myanmar). Especially, Donguibogam, which is traditional korean medicinal book, described the white grub as traditional medicine to treat hepatic diseases and vascular disorders. The white grub has been considered as highly nutritional food. The major constituents of white grub are rich in protein, healthy fats, iron, calcium. Recent studies announced that white grub has hepatoprotective effect and anti-microbacterial effect. However, the immuno-enhancing effect of white grub extracts in RAW 264.7 macrophage cells has not been studied yet. In this study, the various concentrations of white grub extract were examined to find immuno-enhancing effects on RAW 264.7 cells. Cytotoxicity was determined by MTT assay and immuno-enhancing effect of white grub extract was investigated by measuring nitric oxide (NO) production compared with only lipopolysaccharide (LPS) treatment. White grub extracts (0.001 - 10 mg/ml) did not show cytotoxicity. Additionally, white grub extracts (0.001 - 1mg/ml) had Immuno-enhancing effect on RAW 264.7 cells compared with only LPS treated group. These results might be provided proof to develop beneficial immuno-enhancing material for human health.

• Asian Journal of Turfgrass Science
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• v.12 no.3
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• pp.225-236
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• 1998

The white grubs in turfgrass were investigated from 15 golf clubs in 7 provinces and 2 metropolitan cities. 12 white grub species were collected and 11 species in 8 genera including oriental beetle, Anomala orientalis were identified but 1 species was not identified. The oriental beetle grub was the most serious pest in turfgrass out of them. The brown chafer, Adoretus tenuimaculatus was widely distributed species collected from 6 golf clubs, Maladera castanea was collected from 5 golf clubs and A. orientalis, Holotrichia kiotoensis, and M. orientalis were collected from 4 golf clubs. The white grubs, their density, and distribution depth were also observed at tee, fairway, and green from Yongweon, Daegu, and Dongrae golf clubs. A. orientalis was dominant species in overwintering season but A. tenuimaculatus was dominant one in unoverwintering season. The density of white grubs was low in the green compared with tee or fairway. Distribution depth was different depending on season in Daegu and Dongrae golf clubs but not different in Yongweon golf club. Although there showed no differences in white grub species depending on turfgrass but density was different, that is, A. tenuimaculatus was abundant in Zoysia matrella while A. orientalis abundant in Poa pratensis. The density of A. orientalis grubs was higher in older golf courses but there showed no differences in white grub species depending on the age of golf club.

• The Korean Journal of Soil Zoology
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• v.6 no.1_2
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• pp.37-44
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• 2001

Scarabaeids and white grubs were investigated at Halla arboretum and nursery in Jeju province in 1999 and 2001. Scarabaeid pests were surveyed from 14 tree species of 12 families at Halla arboretum in Jeju in March, 1999 and from 55 species of 27 families in March and September, 2001 and from Taxus cuspidata at nursery in Seoguipo in March, 1999 and 18 tree species of 9 families at the same nursery in March and September, 2001. Six species of white grubs and scarabaeids were collected from Halla arboretum. At Halla arboretum, Holotrichia niponensis was collected from 28 tree species of 21 families, H. diomphalia from 27 tree species of 20 families, and an unidentified white grub from 27 tree species of 19 families. Maladera orientalis was collected from 4 tree species of 4 families and Aphodius sp. and Adoretus tenuimaculatus from Punica granatum and Carpinus sieboldiana. At Halla nursery in Seoguipo, H. niponensis were collected from 9 tree species of 6 families and H. diomphalia from 10 tree species of 6 families. White grub of Minela testaceopes was collected from Ternsstraemia japonica. H. niponensis, H. diomphalia and an unidentified white grub were dominant species out of collected white grubs and scarabaeids and these were collected from Cinamomum camphrora. H. niponensis was the most serious pest on T. cuspidata.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2004.05a
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• pp.127-127
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• 2004

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.2
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• pp.77-85
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• 2006

Entomopathogenic nematodes (EPNs) have been used as biological control agents for control of various agro-forest insect pests, and are especially effective against soil-dwelling insect pests. Effect of soil moisture on pathogenicity of commercial EPNs for white grub control was evaluated in laboratory, pots, and golf courses. Pathogenicity of EPNs in sand column was variable depending on depth, soil moisture, and EPN species or strain. All tested EPNs (Heterorhabditis sp. GSNUH1, Heterorhabditis sp. GSNUH2, Steinernema carpocapsae GSN1, and S. longicaudum Nonsan strain) showed similar pathogenicity against the bait insect, great wax moth (Galleria mellonella) larva at 2 cm deep at a given soil moisture. However, pathogenicity of the Heterorhabditis sp. GSNUH1 strain was decreased with increasing soil moisture. Pathogenicity of S. carpocapsae GSN1 strain was the lowest in 3% soil moisture (v/w) at 7 cm depth. However, there was no difference in pathogenicity between Heterorhabditis sp. GSNUH2 and S. longicaudum Nonsan strain. Although pathogenicity of Heterorhabditis sp. KCTC 0991BP strain showed no difference against the 2nd instar of Exomala orientalis, that of the S. carpocapsae GSN1 strain was decreased in the laboratory depending on soil moisture. Highly pathogenic strain EPN, Heterorhabditis sp. KCTC 0991BP strain, showed higher pathogenicity at 100 mm irrigation than non-irrigation or 10 mm irrigation. However, poor pathogenic strain EPN, S. carpocapsae GSN1 strain, was not different in pathogenicity from the 2nd instar of Exomala orientalis in creeping bentgrass (Agrostis palustris) depending on irrigation amount in the pot. Pathogenicity of EPNs in field experiment at the tee of Ulsan golf club showed a similar trend to that in the pot experiment.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.129-134
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• 2015

White grub damages various crops, trees and they can also be one of the most serious pests of turf grass in golf courses. This study was conducted to determine the biocontrol of white grubs with 18 different kinds (anise, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, and turpentine) of plant essential oils in laboratory, green house and field. Anise oil (88.9%), linseed oil (100%), and tea-tree oil (88.9%) were highly effective among tested essential oils against 3rd instar of Bifurcanmala aulax in cap vial experiment. However, anise and linseed oils showed low mortality against $3^{rd}$ instar of Popillia japonica in pot greenhouse experiment. Efficacy of anise, linseed oil, and tea-tree was also different depending on target white grub in field trials. Correlated mortality showed 32.6% only in tea-tree oil treatment against pupae of Adoretus tenuimaculatus however, correlated mortality of anise, linseed and tea-tree oil were 54.8, 51.6 and 56.5% respectively against $3^{rd}$ instar of Exomala orientalis in the field trial in Adelscott Country Club in Hapcheon, Gyeongnam province.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.165-168
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• 2007

This experiment was conducted to investigate population dynamics of white grubs and its damages in sweet potato fields. There were three species of white grubs that fed on the roots of sweet potato in Honam area. Among them, Holofrichia parallela was a major insect pest. Damage rate of sweet potato by white grubs were about 2 to 40% differed with regions. In some region where it was severe the damage rate of sweet potato was about 80% or more. H. parallela overwintered as a late 3rd instar larvae in soil from late October to late-June, and the survival rate of them was 92%. The occurrence pattern of H. parallela larvae varied in different seasons. In sweet potato field, H. parallela larvae populations started being observed during late-July to mid.-August. The damage by the grub began to occur late-August in field and lasted to the harvest time.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.28-28
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• 1991

The investigation of the kinds and density of white grubs in relation to soil texture, forages, yearsafter pasture establishment, and management methods in pasture were surveyed in Livestock ExperimentStation, Suwon by digging 5 sites($100cm{\times}100cm$) in a month from April to October during 1985.The results obtained were summarized as follows; No difference was observed in white grub speciesdivesity in relation to soil texture, forages, years after pasture establishment, and management methodof pasture, but the abundance of each species was different from the environmental factors.1. On the soil texture; Miridiba kureana, Popillia uchidai, Holotrichia mmosa and Holotrichia diomphalia occurred in the clay loam field, whereas Anomala corpulenta and Anomala mfocuprea in the sandy loam. 2. On the forage; the density of all species except Popillia uchidai was higher in grass than legume. 3. On the years after pasture extablishment; both of the species diversity and abundance were increased 3 years after pasture establishment and maximized at the 5th year.4. On the management methods of pasture; species diversity and abundance were higher in meadows thanpastures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.38-42
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• 1991

The investigation of the kinds and density of white grubs in relation to soil texture, forages, years after pasture establishment, and management methods in pasture were surveyed in Livestock Experiment Station, Suwon by digging 5 sites($100cm{\times}100cm$) in a month from April to October during 1985. The results obtained were summarized as follows; No difference was observed in white grub species divesity in relation to soil texture, forages, years after pasture establishment, and management method of pasture, but the abundance of each species was different from the environmental factors.1. On the soil texture; Miridiba kureana, Popillia uchidai, Holotrichia mmosa and Holotrichia diomphalia occurred in the clay loam field, whereas Anomala corpulenta and Anomala mfocuprea in the sandy loam. 2. On the forage; the density of all species except Popillia uchidai was higher in grass than legume. 3. On the years after pasture extablishment; both of the species diversity and abundance were increased 3 years after pasture establishment and maximized at the 5th year.4. On the management methods of pasture; species diversity and abundance were higher in meadows than pastures.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.107-116
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• 2006

Korean entomopathogenic nematode strain were evaluated against white grubs, Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa in laboratory. In addition we examined the efficacy of entomopatogenic nematode, Heterorhabditis sp. KCTC 0991BP strain against white grubs in Seaside Golf Club in Gimpo, Gyeonggii and persistence of entomopatogenic nematode in fairway of Anyang Benest Golf Club in Gunpo, Gyeonggii. In laboratory Heterorhabditis bacteriophora Hamyang strain showed 95% mortality against Popillia quadriguttata. H. bacteriophora Hamyang strain and Heterorhabditis sp. KCTC 0991BP induced 80% mortality against 3rd instar of Ectinohoplia rufipes. However, the letter showed only 60% mortality against Phyllopertha diversa. White grub density was reduced to 39% by the application of Heterorhabditis sp. KCTC 0991BP at the rate of $2.24{\times}10^9$ infective juveniles $ha^{-1}$ compared with control in Seaside Golf Club. Heterorhabditis sp. KCTC 0991BP persisted for two months at $5{\sim}10$ cm soil depth but not persisted at $0{\sim}5$ cm soil depth for the same periods when applied at the rate of $2.47{\times}10^9$ infective juveniles $ha^{-1}$.