The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Pathogenicity of Korean Entomopathogenic Nematodes on Larva of Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa (Coleoptera: Rutelidae) and Persistence in Golf Courses

한국산 곤충병원성 선층의 녹색콩풍뎅이(Popillia quadriguttata), 주황긴다리풍뎅이(Ectinohoplia rufipes), 연다색풍뎅이(Phyllopertha diversa) 유충에 대한 병원성과 골프장에서 지속성

  • Choi, Woo-Geun (Seowon Valley Golf Club) ;
  • Ha, Pan-Jung (SESIL Cooperation) ;
  • Lee, Sang-Myeong (Southern Forest Research Center, National Forest Science Institute) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environment Sciences, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Dong-Woon (Department of Applied Biology, Sangju National University)
  • 최우근 (서원벨리골프장) ;
  • 하판정 ((주)세실) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 추호렬 (경상대학교 응용생물환경학과, 농업생명과학연구원) ;
  • 이동운 (상주대학교 생물응용학과)
  • Published : 2006.06.30
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Abstract

Korean entomopathogenic nematode strain were evaluated against white grubs, Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa in laboratory. In addition we examined the efficacy of entomopatogenic nematode, Heterorhabditis sp. KCTC 0991BP strain against white grubs in Seaside Golf Club in Gimpo, Gyeonggii and persistence of entomopatogenic nematode in fairway of Anyang Benest Golf Club in Gunpo, Gyeonggii. In laboratory Heterorhabditis bacteriophora Hamyang strain showed 95% mortality against Popillia quadriguttata. H. bacteriophora Hamyang strain and Heterorhabditis sp. KCTC 0991BP induced 80% mortality against 3rd instar of Ectinohoplia rufipes. However, the letter showed only 60% mortality against Phyllopertha diversa. White grub density was reduced to 39% by the application of Heterorhabditis sp. KCTC 0991BP at the rate of $2.24{\times}10^9$ infective juveniles $ha^{-1}$ compared with control in Seaside Golf Club. Heterorhabditis sp. KCTC 0991BP persisted for two months at $5{\sim}10$ cm soil depth but not persisted at $0{\sim}5$ cm soil depth for the same periods when applied at the rate of $2.47{\times}10^9$ infective juveniles $ha^{-1}$.

한국산 곤충병원성 선충의 녹색콩풍뎅이(Popillia quadriguttata), 주황긴다리풍뎅이(Ectinohoplia rufipes) 및 연다색풍뎅이(Phyllopertha diversa)에 대한 병원성을 실내에서 검정하였으며 아울러 Heterorhabditis sp. KCTC 0991BP 계통을 이용하여 굼벵이 방제효과와 지속성을 조사하였다. 실내실험 결과 녹색콩풍뎅이 3령충에 대하여 H. bacteriophora Hamyang 계통은 95%의 높은 치사율을 보였으며 주황긴다리풍뎅이 3령충에 대해서도 H. bacteriophora Hamyang 계통과 Heterorhabditis sp. KCTC 0991BP 계통이 80% 이상의 치사율을 나타내었다. 그러나 연다색풍뎅이는 병원성이 가장 높은 Heterorhabditis sp. KCTC 0991BP 계통 처리 시에도 60%의 치사율을 보였다. 골프장에서 Heterorhabditis sp. KCTC 0991BP 계통을 $2.24{\times}10^9$ Ijs $ha^{-1}$ 농도로 처리하였을 때 굼벵이의 밀도는 39%까지 감소되었고, 골프장 페어웨이에서 지속성은 $5{\sim}10$ cm 깊이의 토양에서 두 달까지 지속되었으나 $0{\sim}5$ cm 깊이에서는 치사되는 미끼곤충(꿀벌부채명나방)이 없어 지속성이 낮았다.

Keywords

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