• Journal of Korean Medicine Rehabilitation
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• v.33 no.3
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• pp.47-65
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• 2023

Objectives We evaluated the wound healing effects of Dangguijakyak-san (DJ) using C57BL/6 mice that were generated open wound. Methods The study was conducted with seven C57BL/6 mice assigned to each group, divided into the normal group, control group, vitamin E group, DJ low-dose group, DJ high-dose group. We measured total polyphenol, flavonoid contents, the size of the wound, liver function, pro-inflammatory cytokine activity in serum, inflammation-related proteins, adhesion molecules and chemokine proteins, collagen-related proteins in skin tissue and histopathological changes by H&E and Masson's staining. Results DJ treatment significantly reduced the area of the wound compared to the control group. Also, inflammatory cytokines were reduced and the expression of anti-inflammatory-related factors (interleukin-4 [IL-4] and IL-10) was significantly increased in the DJ treatment group. We identified that DJ treatment inhibits both pathways of inflammation, the mitogen-activated protein kinases and nuclear factor-κB pathway. Moreover, the protein expressions of Sirt1 (sirtuin 1), MCP-1 (monocyte chemoattractant protein 1), ICAM-1 (intercellular adhesion molecule 1), and VCAM-1 (vascular cell adhesion molecule 1) were decreased by DJ administration. Also, the expression of α-smooth muscle actin and collagen type I alpha 1, collagen-related proteins, that help skin recovery was significantly increased in the DJ treatment group. Histopathologically, a relatively thin epithelial layer could be observed in the DJ administration group, as well as an increase in fibroblasts and collagen fibers. Conclusions These data suggest that DJ treatment is effective in wound healing, suppressing inflammatory proteins, increasing skin repair factors and improving histopathological changes caused by wounds.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1757-1763
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• 2011

The physico-chemical properties of broccoli sprouts cultivated in a plant factory system with different lighting conditions were investigated. We reported that there were significant differences among the chemical and nutritional properties of the broccoli sprouts grown under different light sources. Two kinds of sugars (glucose and fructose) were detected in the plants. The amount of glucose was 2.94~3.12% and that of fructose was 1.54~2.04%. Total chlorophyll was $1,157{\pm}0.004$ mg% and chlorophyll-a $777{\pm}0.01$ mg%. All over the test materials, 2 kinds of organic acids (citric acid and malic acid) were detected. The level of citric acid was 908~1,136 mg% and its highest level was under the (Blue LED) light source. The level of malic acid was 514~834 mg% and its highest level was under the (Red LED) light source. Seven different minerals were also analyzed. The amount of K was 518 mg% and its amount was significantly higher than that of Mg or Na. There were also negligible amounts of Zn, Fe and Cu. The amounts of vitamin A, C and E under the Red LED were, $860.62{\pm}0.02\;{\mu}gRE$, $134.570{\pm}0.14$ mg% and $1.44{\pm}0.1$ IU. The amounts under the Blue LED were, $432.48{\pm}0.05\;{\mu}gRE$, $137.05{\pm}0.1$ mg% and $1.11{\pm}0.12$ IU. The amounts under both Red and Blue LEDs were, $667.33{\pm}0.11\;{\mu}gRE$, $118.50{\pm}0.09$ mg% and $1.47{\pm}0.1$ IU. And finally, the amounts under a White LED were, $640.25{\pm}0.08\;{\mu}gRE$, $119.87{\pm}0.07$ mg% and $1.31{\pm}0.15$ IU. In this study, significant changes were shown in the chemical and nutritional properties of the broccoli sprouts. These findings indicate that LED light sources stimulated germination of the plants.

• Horticultural Science & Technology
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• v.29 no.3
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• pp.247-254
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• 2011

The agronomic characteristics, nutritional contents, and insect response of the potato clones transformed with a glufosinate ammonium resistance gene were evaluated. Among the 4 transgenic potato clones, the Bar 3 clone was selected as a promising one for commercialization. The Bar 3 clone showed similar tuber yield capacity but higher herbicide resistance as compared with the non-transgenic potato cv. Dejima. The herbicide resistance of the Bar 3 clone was more than 5 times higher when tested with the herbicide concentration recommended by the producer. The major agronomic characteristics of the Bar 3 clone were not different from those of the non-transgenic Dejima. The annual variation in yields and agronomic characteristics showed similar tendency for 2 years from the third to fourth generation after transformation. The tubers of the Bar 3 clone also showed low occurrence in common scab and physiological disorders such as cracking and secondary growth. But the reasons for such results are yet to be studied. Also, it was considered that the Bar 3 clone have a potential of reducing not only common scab occurrence but also soil erosion during potato cultivation in field. The nutritional contents (mineral compound, vitamin C and amino acid) and response to Spodoptera exigua of the transgenic potato clones were not significantly different.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.4
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• pp.537-543
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• 2014

Three different granule powders of Citrus Hallabong were prepared with different ratios of fresh juice and citric acid (J10C3=10.0:3.08, J08C4=8.75:4.33, J07C5=7.5:5.58) at fixed quantities of juice powder (70%), pressed cake powder (10%), and additives (6.92%), and their physicochemical properties were measured and compared with targeted commercial product (CP). The moisture content of Hallabong granules (HG) was 5.08~5.44% (w/w), which was two times higher than that of CP. Titratable acidities increased with higher citric acid content and were 1.7~2.3 times lower in HG compared to that of CP. Soluble solids of HG increased with higher citrus juice content ($90{\sim}98^{\circ}Brix$) and were slightly lower than that of CP. Vitamin C contents of HG increased with higher citrus juice content and were 5.8~7.6 times lower than that of CP. In terms of color difference, L and b values of HG were higher while a value was lower than those of CP. Bulk and compaction densities of HG were 0.541~0.660 g/mL and 0.561~0.689 g/mL, respectively and were similar to those of CP. Water solubility and swelling capacity were 66.6~72.0% and 3.84~6.40 g/g, respectively, and were similar to those of CP. Hygroscopicity of HG after an hour of elapsed time was 1.6~1.8 times higher than that of CP. Sensory evaluation test showed that color, sweetness, and overall acceptance of HG were not significantly different from those of CP, whereas flavor and bitterness of HG were lower than those of CP. In conclusion, convenient food granules could be made with Citrus Hallabong juice and pressed cake, which is similar to the commercial product.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.10
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• pp.1484-1491
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• 2015

The new cultivar Lentinula edodes, which is named 'Lentinula edodes GNA01', was bred by mating strains isolated from 'L26' and 'Kyoungwon9015' obtained from Sammyungjin Research Institute, Fujian, China. L. edodes GNA01 does not have stipes like L. edodes, although it generally has a similar spherical shape. Moisture and crude protein contents of L. edodes GNA01 were lower than those of L. edodes. Meanwhile, L. edodes GNA01 contained higher levels of crude ash, crude lipid, crude fiber, and carbohydrates than L. edodes. The ${\beta}$-carotene content ($19.05{\mu}g/100g$) of L. edodes GNA01 was about three times higher than that of L. edodes. In addition, vitamin D content ($118.53{\mu}g/100g$) of L. edodes GNA01 was more than twice that of L. edodes. L. edodes GNA01 was a good source of mineral elements, with K and Mg contents of 2,277.50 mg/100 g and 203.15 mg/100 g, respectively. The major fatty acids of L. edodes GNA01 were C16:0 and C18:2, and L. edodes GNA01 had the highest linoleic acid (C18:2) content of 1,087.66 mg/100 g. Total phenol content of L. edodes GNA01 was 12.52 mg GAE/g, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) values of L. edodes GNA01 were lower than those of L. edodes at all concentrations. However, DPPH radical scavenging activities and FRAP values of L. edodes GNA01 were above 80% and 0.9 at a concentration of 10 mg/mL, respectively.

• Korean Journal of Food Science and Technology
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• v.36 no.2
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• pp.333-338
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• 2004

Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Corner officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from $2.6\;(Polygonatum\;odoratum){\sim}81.2(Epimedium\;koreanum)\;mg/g$. Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. $IC_{50}$ for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.

• Journal of agriculture & life science
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• v.46 no.4
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• pp.129-139
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• 2012

This study was carried out to evaluate the chemical properties and functional characteristics, such as general composition and bioactivity compounds contents of fresh and blanched (at $95^{\circ}C$, for 5min) garlic shoot from Namhae. Also, evaluated antioxidant and antimicrobial activities of water and ethanol extract of fresh and blanched garlic shoot. The moisture content of fresh garlic steam was $ 85.14{\pm}0.35%$, crude protein and crude lipid were $0.79{\pm}0.26%$ and $2.96{\pm}0.03%$ respectively. Vitamin C content was higher in fresh garlic shoot ($7.07{\pm}0.84mg/100g$) than blanched. Total phenol and total chlorophyll contents were respectively $16.93{\pm}1.17mM/g$ and $6.70{\pm}0.46mg/g$ in fresh garlic shoot. Allicin content of blanched garlic shoot was $128.63{\pm}1.59mM/g$. This content was 1.82 times higher than the fresh garlic shoot. Total pyruvate content was higher in fresh garlic shoot ($24.63{\pm}1.59mM/g$), but thiosulfinate was higher in fresh garlic shoot. Total flavonoide was the highest in water extract of blanched garlic shoot ($3.67{\pm}0.00mM/g$). ABTS radical scavenging activity of water extract form blanched garlic shoot was $85.09{\pm}0.28%$, which was higher than the other extracts. NO radical scavenging activity of ethanol extract from blanched garlic shoot was significantly higher than the extracts from fresh garlic shoot. Antibacterial activity to S. aureus, S. enterica, B. cereus and E. coli was only indicated in water extract of fresh garlic shoot.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.10
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• pp.1488-1495
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• 2015

This study was conducted to investigate the effects of post-harvest storage duration and wheat variety on the digestibility and energy content of new season wheat fed to finishing pigs. Two wheat varieties (Shi and Zhong) were harvested in 2013 and stored in the warehouse of the Fengning Pig Experimental Base at China Agricultural University for 3, 6, 9, or 12 mo. For each storage period, 12 barrows were placed in metabolism crates and allotted to diets containing 1 of the 2 wheat varieties in a randomized complete block design. The experimental diets contained 97.34% wheat and 2.66% of a vitamin and trace mineral premix. With an extension of storage duration from 3 mo to 12 mo, the gross energy (GE) and crude protein (CP) of the wheat decreased by 2.0% and 12.01%, respectively, while the concentration of neutral detergent fiber (NDF), acid detergent fiber (ADF) and starch content increased by 30.26%, 19.08%, and 2.46%, respectively. Total non-starch polysaccharide, total arabinose, total xylose and total mannose contents decreased by 46.27%, 45.80%, 41.71%, and 75.66%, respectively. However, there were no significant differences in the chemical composition between the two wheat varieties with the exception of ADF which was approximately 13.37% lower in Shi. With an extension of storage duration from 3 mo to 12 mo, the digestible energy (DE), metabolizable energy (ME) content and the apparent total tract digestibility of GE, CP, dry matter, organic matter, ether extract, ADF and metabolizability of energy in wheat decreased linearly (p<0.01) by 5.74%, 7.60%, 3.75%, 3.88%, 3.50%, 2.47%, 26.22%, 27.62%, and 3.94%, respectively. But the digestibility of NDF changed quadratically (p<0.01). There was an interaction between wheat variety and storage time for CP digestibility (p<0.05), such that the CP digestibility of variety Zhong was stable during 9 mo of storage, while the CP digestibility of variety Shi decreased (p<0.05). In conclusion, the GE, DE, and ME of wheat was stable during the first 3 to 6 mo of post-harvest storage, and decreased during the following 6 to 12 mo of storage under the conditions of this study.

• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.371-376
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• 2009

The fruits of sweet cherry are highly appreciated by the consumer due to their precocity and quality, such as their sweetness, color and sourness. In this study, the hot-water extract and its sequential organic solvent fractions were prepared from domestic Napoleon sweet cherry (Prunus avium L.) to investigate antimicrobial and antioxidant activity. The hot-water extract contained about 40% sugars, and the solvent fraction yields for hexane, ethylacetate (EA), butanol, and water residue were 0.01%, 3.45%, 16.30%, and 80.24%, respectively. Contents of total polyphenol and total flavonoid of the fractions were 1.24~5.24%, and 0~3.76%, respectively. Among the fractions, EA fraction showed the highest total polyphenol and total flavonoid concentrations. Evaluation of antimicrobial activity of the extract and fractions revealed that EA fraction and butanol fraction contained strong antibacterial activity against Listeria monocytogenes, Staphylococcus aureus, and Salmonella typhimurium with minimal inhibitory concentration (MIC) of 0.5~1.0 mg/mL. But the extract and fractions tested were not active to Pseudomonas aeruginosa. In a while, only hexane fraction showed anti-Candida activity with 0.5~1.0 mg/mL of MIC. The fraction showed strong activity against different multi-antibiotics resistant strains such as C. albicans CCARM 14020. Antioxidative activity assay showed that EA fraction has a strong DPPH scavenging activity and a reducing power. The $IC_{50}s$ of vitamin E and EA fraction were 15.5 and $195.5\;{\mu}g/mL$, respectively. Our results suggest that the fruit of P. avium L. has high potentials with anti-Candida and antioxidative activity.

• Journal of Nutrition and Health
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• v.25 no.6
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• pp.511-517
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• 1992

The hypoglycemic effects of Korean wild vegetables : Capsella bursa-pastoris Medicus(CBM) Commelina communis L. (CCL) Calystegics japonica Choisy(CJC) Discorea japonica Thunb(DJT) and Persicaria perfolidata Gross(PPG) in diabetic rats were determined. Sixty male Sprague-Dawley diabetic rats(130~180g) induced by the streptozotocin (45mg/kg) injection into the tail vein were fed either a control or experimental diets for four weeks. The plasma levels of glucose and cholesterol were measured. The urinary glucose levels were monitored. Crude fiber mineral(Ca, Fe, Zn and Cr) and ascorbic acid contents of the wild vegetables were analyzed. The extents of blood glucose decrement in CCL. DJT or PPG fed rats were greater than that in the control group. This extents of decrement in CBM or CJC fed rats were not significan-tly different from that in the control group. The urinary glucose was shown to be negative to Band reagent strip in CCL or DJT group at the 4th week. The plasma cholesterol levels of all the groups including control group were not essentially different. It is suggested that the intakes of CCL or DJT could be useful for prevetive and therapeutic approches to alleviate the hyperglycemic status in diabetes mellitus.