• Journal of fish pathology
• /
• v.23 no.1
• /
• pp.37-45
• /
• 2010

In this study, we carried out research on the level of single and multi-drug resistance of bacteria isolated from cultured flounder Paralichthys olivaceus. One hundred sixty one bacteria were isolated from cultured olive flounder Paralichthys olivaceus in Korea and the isolates consisted of Edwardsiella tarda (n=32), Vibrio ichthyoenteri (n=37), Vibrio spp. (n=54), Streptococcus parauberis (n=28) and Streptococcus spp. (n=10). These E. tarda isolates were highly resistant in the order of tetracycline (84.4%) and oxolinic acid (71.9%). V. ichthyoenteri and Vibrio spp. showed resistance ampicillin (94.6% and 81.5%) and tetracycline (56.8% and 42.6%). S. parauberis isolates were resistant ampicillin (57.1%), tetracycline (57.1%) and erythromycin (35.7%). Of the isolates, 84.4% of E. tarda, 73.0% of V. ichthyoenteri, 57.4% of Vibrio spp., 42.8% of S. parauberis and 70.0% of Streptococcus spp. isolates exhibited multi-drug resistance against more than two antibiotics.

• Fisheries and Aquatic Sciences
• /
• v.24 no.6
• /
• pp.207-218
• /
• 2021

This study evaluated the risk of foodborne illness from highly pathogenic Vibrio spp. (Vibrio vulnificus and V. cholerae) by raw whip-arm octopus (Octopus minor) consumption. In total 180 samples of raw whip-arm octopus were collected from markets and examined for the prevalence of V. vulnificus and V. cholerae. Predictive models describing the kinetic behavior of Vibrio spp. in raw whip-arm octopus were developed, and the data on amounts and frequency of raw whip-arm octopus consumption were collected. Using the collected data, a risk assessment simulation was conducted to estimate the probability of foodborne illness raw whip-arm octopus consumption using @RISK. Initial contamination levels of Vibrio spp. in raw whip-arm octopus were -3.9 Log colony-forming unit/g, as estimated by beta distribution fitting. The developed predictive models were appropriate to describe Vibrio spp. in raw whip-arm octopus during distribution and storage with R² values of 0.946-0.964. The consumption frequency and daily consumption amounts of raw whip-arm octopus per person were 0.47% and 57.65 g, respectively. The probability of foodborne illness from raw whip-arm octopus consumption was estimated to be 8.71 × 10^-15 for V. vulnificus and 7.08 × 10^-13 for V. cholerae. These results suggest that the risk of Vibrio spp. infection from raw whip-arm octopus consumption is low in South Korea.

• Journal of fish pathology
• /
• v.37 no.1
• /
• pp.61-69
• /
• 2024

In this study, bacterial disease monitoring was conducted from July to November 2022 for three representative bacterial species (Streptococcus parauberis, Vibrio spp. and Edwardsiella piscicida) to investigate the occurrence of bacterial diseases in farmed olive flounder (Paralichthys olivaceus) on Jeju. Over a period of five months, 3,146 olive flounders were tested. The average length and weight of the olive flounder that were sampled for the disease were found to be 32.1 cm and 357.9 g, respectively. Bacteria were detected in 1,228 olive flounders, of which 24.6% were identified as Vibrio spp.. S. parauberis and E. piscicida were identified in 6.3% and 3.2% respectively, and 3% were unknown strains that could not be identified. Also, 41 (1.3%) olive flounders were found to have both S. parauberis and Vibrio spp. and 23 (0.7%) olive flounders were found to have both E. piscicida and Vibrio spp.. In particular, S. parauberis, Vibrio spp. (V. scophthalmi) and E. piscicida were detected simultaneously in one olive flounder. In fish infected with E. piscicida, the most obvious external signs were hernias and ascites.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.29 no.1
• /
• pp.9-16
• /
• 1996

In order to understand microbial ecosystem in the fast china sea and Yosu coastal sea horizontal distributions of bacterial flora, Vibrio spp., coliform group, temperature, and salinity in this sea area, were studied 42 sampling stations during 6th-l4th August, 1992. From the results, salinity and temperature were $24.64\~33.78\%_{\circ}$ and $22.53\~29.18^{\circ}C$, respectively. As the open sea far from land goes on, salinity became low while temperature became high. Viable cell counts of bacteral flova, Vibrio spp., and roliform group in Yosu coastal sea water were $1.0\times10^2\~3.0\times10^4/ml,\;0.2\times10\~9.0\times10^3/ml,\;and\;0.3\times10\~3.0\times10^3/ml$ while those of these in the open sea water were $0.4\times10\~2.0\times10^3/ml,\;0.8\times10\~3.0\times10/ml,\;and\;0.9\times10\~1.3\times10/ml$, respectively.

• Journal of environmental and Sanitary engineering
• /
• v.23 no.3
• /
• pp.31-38
• /
• 2008

This study was conducted to investigate the effects of environmental factor such as temperature, salinity, turbidity, pH and dissolved oxygen on the growth of Vibrio spp.. In this survey, total 56 seawater samples were obtained from 8 different sites of the Incheon coastal area during the periods from april 2008 to october 2008. Enumeration of Vibrio spp. was determined by using the most probable number(MPN) procedure. Isolation rates of V. parahaemolyticus, V. vulnificus, V. cholerae in all samples tested were 44.0%, 21.4% and 13.1%, respectively. The enumeration of Vibrio spp. was very low correlated with water temperature and pH and negatively correlated with salinity, dissolved oxygen and turbidity. We found salinity to be the parameter most highly correlated with the enumeration of Vibrio spp. The highest rate of antibiotic resistance of V.vulnificus and V.parahaemolyticus was Cefazolin(11.5%), Ampicillin(70.8%), respectively.

• Journal of fish pathology
• /
• v.20 no.3
• /
• pp.229-235
• /
• 2007

Cell number of Vibrio spp. was examined for the surface and bottom sea water harvested from the 9 stations in the Jeonam Archipelago Marine Ranching Ground in 2006～2007. Species population composition and dominant species were analyzed as well. The largest number of Vibrio spp. was detected in summer (June, 2007) among the 4 seasons with the surface and bottom sea water samples of 5.4～93.2 cfu㎖-1 and 1.0～53.1 cfu㎖-1. respectively. In winter (January, 2007), Vibrio spp. was not detected at all except that one station from which 0.2 cfu㎖-1 of Vibrio spp. was counted in the surface sea water sample. Cell numbers were 0～11.9 cfu㎖-1 and 0～8.3 cfu㎖-1, respectively, in the surface and bottom sea water samples retrieved in spring (April, 2007), and the corresponding sea water samples in autumn(November, 2006) were contaminated with 0.1～21.4 cfu㎖-1 and 0～2.9 cfu㎖-1, respectively. Species population composition of Vibrio spp. was found to be V. parahaemolyticus, V. alginolyticus and V. cholerae non-01 among which V. parahaemolyticus and V. alginolyticus were dominant in all seasons. V. alginolyticus was detected from all stations even in the winter season indicating that this species seems to be less temperature dependent than the other Vibrio spp..

• Journal of Veterinary Clinics
• /
• v.19 no.1
• /
• pp.14-18
• /
• 2002

An epidemiological study was performed to evaluate the state of fish diseases between aquaculture fishes in the area of southern Kyeongnam, especially Tongyeong-si, Geoje-si, and Goseong-gun. Examination for a total of 91 cases was carried out for a variety of fishes, including Japanese flounder(Paralichthys olivaceus), rock fish (Sebastes schlegeli), sea bass (Lateolabrax japonicus), sea breams(Pagrus major), and red drum (Sciaenops ocellatus) from November, 1999 to October, 2001. The investigation resulted in exploring 155 infectious diseases, including 62 Vibriosis, 16 Edwasiella infection, 11 Streptococcosis, 7 Pseudotuberculosis, 15 Scuticociliatida infection, 20 Gill flukes, and 9 Trichodiniasis. When the infections were classified according to fish species, Japanese flounder was infected by 30 Vibrio spp., 16 Edwardsiella tarda, 6 Streptococcus spp., 12 Scuticociliatida infection. In case of sea bass, 10 Vibrio spp. was able to isolate out of 10 outbreaks, on the other hand, rock fish was dominantly infected by gill flukes since it was observed 12 out of a total of 20 cases. With reference to seasonal fluctuation, Vibrio spp. infection was continually observed through all seasons but Edwardsiella septicaemia, Streptococcosis and Pseudotuberculosis was mainly isolated in summer and autumn when the water temperature was raised high. Scuticociliatida infection was frequently occurred from late autumn to early spring but gill flukes were watched all the year regardless of temperature. As regards, multiple infection, 47 out of 91 cases was Identified to have involved in more than 2 pathogens, especially Vibrio spp. was highly mixed with other pathogens since 42 out of 47 cases was mixed with Vibrio spp.

• Journal of fish pathology
• /
• v.20 no.1
• /
• pp.61-70
• /
• 2007

The epidemiological study was performed to survey the prevalence of bacterial disease of cultured olive flounder, Paralichthys olivaceus from October, 2004 to August, 2006 in Korea. A total of 1,271 of fish samples were collected at random includes fish exhibiting clinical signs of the disease in question. The total 331 samples among 738 cases of infectious diseases were infected with 366 bacteria isolates including Vibrio spp. (42.1%), Streptococcus spp. (16.9%), Edwardsiella tarda (12.3%), Photobacterium damselae subsp. damselae (8.2%), Pseudomonas spp. (2.2%) or others (18.3%). Vibrio spp. and P. damselae subsp. damselae were continually isolated through all seasons but Streptococcus spp. and E. tarda were mainly isolated from May to November. The 206 cases were showed mixed infection with other bacteria (3.6%), parasites (31.4%) or virus (41.7%); Vibrio spp. (n=21), Streptococcus spp. (n=13), Trichodina (n=76), Scutica (n=31), VNNV (n=112), VHSV (n=46).

• Korean journal of food and cookery science
• /
• v.12 no.3
• /
• pp.312-319
• /
• 1996

Oyster (Crassostrea virginica) and Weakfish (Cynoscion regalis) were stored at 6, 0, -4 and -20$^{\circ}C$ for up to 45 days and examined for changes in microflora. Aerobic plate counts (incubated at 21$^{\circ}C$) were performed at selected times during storage and 495 isolates (255 isolates from oyster and 240 isolates from Weakfish) were randomly selected from the plates during the storage. Before the storage of the fishes, viable counts of oyster were 4.9${\times}$10$\^$5/ CFU/g of meat and those of Weakfish were 1.5${\times}$10$^4$ CFU/cm$^2$of skin. Microflora of oyster before storage, the major isolates identified as Pseudomonas spp. (67%) and Vibrio spp. (20%). Pseudomonas ll1/1V-H and Flavobacterium/Cytophaga were predominant genus in the microflora of oyster during cold storage at 6, 0, -4 and -20$^{\circ}C$. The composition of the microflora of Weakfish before storage, Acinetobacter (40%) and Moraxella (33%) were the major species, with Pseudomonas and Vibrio constituting a small percentage of the total isolates. The microflora shifted to predominantly Pseudomonas spp. during storage at 6. 0 and -4$^{\circ}C$, making up from 60 to 100％ of isolated strains. During frozen storage, the percentage of isolates identified as Mnraxella increased to 40-60% of the total isolates. During cold storage, halophilic bacteria (Pseudomonas lII/IV-H and Vibrio) were predominant in oyster while nonhalophilic bacteria (Pseudomonas III/IV-NH and Moraxella) were predominant in Weakfish. Vibrio spp. were higher in oyster than in Weak fish. Listeria spp. were not isolated but unidentified ${\beta}$-hemolytic bacteria were islolated from both of the fishes during cold storage.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.47 no.6
• /
• pp.740-744
• /
• 2014

In this study, the ${\beta}$-lactamase (VPA0477) gene was used as a new target for the PCR-based detection of Vibrio parahaemolyticus. Primers specific for the ${\beta}$-lactamase (VPA0477) gene of V. parahaemolyticus, were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and non-Vibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The ${\beta}$-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.