Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Seasonal Distribution of Vibrio spp. in the Jeonam Archipelago Marine Ranching Ground

전남 다도해 바다목장 비브리오속 세균의 계절적 분포

  • Published : 20071200
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Abstract

Cell number of Vibrio spp. was examined for the surface and bottom sea water harvested from the 9 stations in the Jeonam Archipelago Marine Ranching Ground in 2006～2007. Species population composition and dominant species were analyzed as well. The largest number of Vibrio spp. was detected in summer (June, 2007) among the 4 seasons with the surface and bottom sea water samples of 5.4～93.2 cfu㎖-1 and 1.0～53.1 cfu㎖-1. respectively. In winter (January, 2007), Vibrio spp. was not detected at all except that one station from which 0.2 cfu㎖-1 of Vibrio spp. was counted in the surface sea water sample. Cell numbers were 0～11.9 cfu㎖-1 and 0～8.3 cfu㎖-1, respectively, in the surface and bottom sea water samples retrieved in spring (April, 2007), and the corresponding sea water samples in autumn(November, 2006) were contaminated with 0.1～21.4 cfu㎖-1 and 0～2.9 cfu㎖-1, respectively. Species population composition of Vibrio spp. was found to be V. parahaemolyticus, V. alginolyticus and V. cholerae non-01 among which V. parahaemolyticus and V. alginolyticus were dominant in all seasons. V. alginolyticus was detected from all stations even in the winter season indicating that this species seems to be less temperature dependent than the other Vibrio spp..

2006～2007년 전남 다도해 바다목장 내 9개 정점을 대상으로 표층수와 저층수에서 비브리오속 세균의 개체수를 측정하고, 군집의 종조성 및 우점종을 분석하였다. 계절별 비브리오속 세균의 개체수는 여름(2007년 6월) 표층수에서 5.4～93.2 cfu㎖-1, 저층수에서 1.0～53.1 cfu㎖-1로 4계절 중 가장 많이 검출되었으며, 해수온도가 낮은 겨울(2007년 1월)의 경우에는 1개 정점의 표층수에서만 0.2 cfu㎖-1의 비브리오속 세균이 검출되었다. 봄(2007년 4월) 조사에서 표층수는 0～11.9 cfu㎖-1, 저층수에서 0～8.3 cfu㎖-1, 가을(2006년 11월) 조사의 표층수는 0.1～21.4 cfu㎖-1, 저층수에서는 0～2.9 cfu㎖-1의 비브리오속 세균이 검출되었다. 비브리오속 세균 군집의 종조성은 V. parahaemolyticus, V. alginolyticus 및 V. cholerae non-01으로 조사되었으며, V. parahaemolyticus와 V. alginolyticus가 4계절 모두 분리되어 우점종으로 나타났다. V. alginolyticus는 수온이 낮은 겨울에도 대부분의 정점에서 분리되어 다른 비브리오속 세균에 비하여 온도에 덜 민감한 종인 것으로 조사되었다.

Keywords

References

  1. Baffone, W., Vittoria, E., Campana, R., Citterio, B., Casaroli A. and Pierfelici, L.: Occurrence and expression of virulence-related properties by environmental halophilic Vibrio spp. in vitro and in vivo systems. Food Control. 16:451-457, 2005 https://doi.org/10.1016/j.foodcont.2004.05.006
  2. Bej. A. K., Patterson, D. P., Brasher, C. Y., Vickery, M. C. L., Jones. D. D. and Kaysner, C. A.: Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J. Microbiol. Med., 36:215-222, 1999 https://doi.org/10.1016/S0167-7012(99)00037-8
  3. Byun, K. D., Lee. J. H., Lee. K. J. and Kim. S. J.: Rapid and specific detection of virulent Vibrio vulnificus in tidal flat sediments. Kor. J. Microbiol., 41: 178-176. 2005
  4. Charles. A. K. ancl DePaola. J. A.: V. cholerae, V. parahaemolyticus. V. vulnificus and Other Vibrio spp. In: Food and Drug Administration, Bacteriological Analytical Manual. 8th ed., revision A(l998). Ch, 9. 2004
  5. Choi, Y. J. and Tak, R.: Survival of Vibrio parahaemolyticus in marine environmentals suring winter season. Kor. J. Pub. Health. 15(3): 219-230. 1991
  6. Eddy, S. D. and Jones. S. H.: Microbiology of summer flounder Paralichthys dentatus fingerling production at a marine fish hatchery. Aquaculture.211: 9-28, 2002 https://doi.org/10.1016/S0044-8486(01)00882-1
  7. Hijarrubia, M. J., Lazaro, B., Sunen, E. and Fernandez- Astorga, A.: Survival for Vibiro vulnificus under pH, salinity and temperature combined stress. Food Microbiol., 13: 193-199, 1996 https://doi.org/10.1006/fmic.1996.0024
  8. Jung, K. J. and Shin, S. U.: Bacterial flora of East China sea and Yosu coastal areas. l. Horizontal distributions according to number of bacteria, Vibrio spp. and coliform group. J. Kor. Fish. Soc., 29, 9-16, 1996
  9. Kang, B. J.: A study on the characteristics of bacteria isolated from cultured flouders(Paralichthys olivaceus) showing disease symptoms in Jeju area of Korea. Jeju Natl. Univ. Ph.D. thesis. 2003
  10. Kim, S. M., Won, K. M., Woo, S. H., Li, H., Kim, E. J., Choi, K. J., Cho, M. Y., Kim, M. S. and Park. S. I.: Vibrios isolated from diseased marine culturing fishes in Korea. J. Fish Pathol., 18: 133-145. 2005
  11. Lee, H., Oh, Y. H., Park, S. G. and Choi. S. M.: Antibiotic susceptibility and Distribution of Vibrio parahaemoiyticus isolated from the seafood. Kor. J. Environ. Health., 33(1): 16-20, 2007 https://doi.org/10.5668/JEHS.2007.33.1.016
  12. Lee, S. K. Y., Wang, H. Z., Law, S. H. W., Wu, R. S. S. and Kong, R. Y. C.: Analysis of the 16S23S rDNA intergenic spacers(IGSs) of marine vibriosis for species-specific signature DNA sequences. Mar. Poll. Bull., 44:412-420, 2002 https://doi.org/10.1016/S0025-326X(01)00256-9
  13. Nhung, P. H., Shah, M. M., Ohkusu, K., Hata, M. N. H., Sun. X. S., Iihara, H., Goto, K., Masaki, T., Miyasaka, J. and Ezaki, T.: The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species. Syst. Appl. Microbiol., 30: 309-315, 2007 https://doi.org/10.1016/j.syapm.2006.11.004
  14. Panicker, G., Douglas, R. C. Melissa, J. K. and Bej, A. K.: Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays. Appl. Environ. Microbiol., 70: 7436-7444, 2004 https://doi.org/10.1128/AEM.70.12.7436-7444.2004
  15. Raghunath, P., Karunasagar, I. and Karunasagar, I.: Evaluation of an alkaline phosphataselabeled oligonucleotide probe for detection and enumeration of Vibrio spp. from shrimp hatchery environment. Mol. and Cell. Probes, 21: 312-315, 2007 https://doi.org/10.1016/j.mcp.2007.03.003
  16. Shin, S. U, Jeong. W. W., Kang, T. J. and Kang, S. K.: Studies on seasonal variation of the bacterial flora in sea-water of Kamak bay, l. On the pathogenic Vibrio and Salmonella. Bull. Mar. Sci. Inst., 1:29-37, 1992
  17. Son, K. T, Oh, E. G., Lee, T. S., Lee, H. J., Kim, P. H. and Kim, J. H.: Antimicriobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from farms on the southern coast of Korea. J. Kor. Fish Soc., 38: 365-371, 2005
  18. Stabili, L., Acquaviva, M. I. and Cavallo, R. A.: Mytilus galloprovincialis filter feeding on the bacterial community in a Mediterranean coastal area (Northern Ionian Sea. Italy). Water Res., 39:469-477, 2005 https://doi.org/10.1016/j.watres.2004.10.010
  19. Su, Y. C. and Liu, C.: Vibrio parahaemolyticus: A concern of seafood safety. Food Microbiol., 24: 549-558. 2007 https://doi.org/10.1016/j.fm.2007.01.005
  20. Thompson, F. L., Austin, B. and Swings, J.: The Biology of Vibrios. Amer. Society for Microbiology. 1st ed, pp. 384, 2006