Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Monitoring of pathogenic bacteria in cultured olive flounder (Paralichthys olivaceus) of Jeju during the summer of 2022

2022년 하절기 제주도 양식 넙치의 병원성 세균 모니터링

  • Ye Ji Kim (Jeonju Regional Office, National Fishery Products Quality Management Services) ;
  • Lyu Jin Jun (Department of Marine Life Sciences, Jeju National University) ;
  • Young Juhn Lee (Department of Marine Life Sciences, Jeju National University) ;
  • Yeong Eun Oh (Department of Marine Life Sciences, Jeju National University) ;
  • Eung Jun Lee (Department of Marine Life Sciences, Jeju National University) ;
  • Joon Bum Jeong (Department of Marine Life Sciences, Jeju National University)
  • 김예지 (국립수산물품질관리원 전주지원) ;
  • 전려진 (제주대학교 해양생명과학과) ;
  • 이영준 (제주대학교 해양생명과학과) ;
  • 오영은 (제주대학교 해양생명과학과) ;
  • 이응준 (제주대학교 해양생명과학과) ;
  • 정준범 (제주대학교 해양생명과학과)
  • Received : 2024.05.13
  • Accepted : 2024.06.10
  • Published : 2024.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, bacterial disease monitoring was conducted from July to November 2022 for three representative bacterial species (Streptococcus parauberis, Vibrio spp. and Edwardsiella piscicida) to investigate the occurrence of bacterial diseases in farmed olive flounder (Paralichthys olivaceus) on Jeju. Over a period of five months, 3,146 olive flounders were tested. The average length and weight of the olive flounder that were sampled for the disease were found to be 32.1 cm and 357.9 g, respectively. Bacteria were detected in 1,228 olive flounders, of which 24.6% were identified as Vibrio spp.. S. parauberis and E. piscicida were identified in 6.3% and 3.2% respectively, and 3% were unknown strains that could not be identified. Also, 41 (1.3%) olive flounders were found to have both S. parauberis and Vibrio spp. and 23 (0.7%) olive flounders were found to have both E. piscicida and Vibrio spp.. In particular, S. parauberis, Vibrio spp. (V. scophthalmi) and E. piscicida were detected simultaneously in one olive flounder. In fish infected with E. piscicida, the most obvious external signs were hernias and ascites.

2022년 7월부터 11월까지 제주 지역의 다양한 넙치양식장을 대상으로 세균성 질병 검사를 수행하였다. 검사를 진행한 3,146마리 중 세균이 검출되지 않은 1,918마리를 제외한 1,228마리에서 검출된 세균을 동정하였다. 그 결과, 773마리(24.6%)에서 Vibrio spp.가 검출되었으며, 그 중 34.8%가 V. harveyi로 확인되었다. S. parauberis와 Vibrio spp.가 동시에 검출된 넙치는 41마리(1.3%)로 나타났고, E. piscicida와 Vibrio spp.가 같이 검출된 넙치는 23마리(0.7%)로 확인되었다. 특히, 한 개체에서 S. parauberis와 Vibrio spp. (V. scophthalmi) 그리고 E. piscicida가 동시에 검출된 경우를 확인할 수 있었다.

Keywords

Acknowledgement

이 논문은 2023학년도 제주대학교 교원성과지원사업에 의하여 연구되었음.

References

  1. An, N., Lee, J.K., Lee, J.S. and Choi, K.H.: Effect of oxygen micro-bubble for the temperature and oxygen concentrations of fish farming facility. J. Korean Soc. Fish. Ocean Technol., 56(4): 407-418, 2020. https://doi.org/10.3796/KSFOT.2020.56.4.407
  2. Cho, K.T., Hwang, Y.J., Lee, S.W., Kim, K.I. and Jeong, H.D.: Distribution of tetracycline-resistance genes detected from isolates of cultured fishes in Gyeonggi-do. J. Fish. Pathol., 34(1): 39-46, 2021. https://doi.org/10.7847/jfp.2021.34.1.039
  3. Cho, M.Y., Kim, M.S., Choi, H.S., Park, G.H., Kim, J.W., Park, M.S., and Park, M.A.: A statistical study on infectious diseases of cultured olive flounder, Paralichthys olivaceus in Korea. J. Fish. Pathol., 21(3): 271-278, 2008.
  4. Choi, D.Y., Choi, H.J., Jo, A.H., Lee, B.Y., Seo, J.W., Na, J.H., Park, J.W., Kim, J.H. and Kang, Y.J.: Effect of disinfectant consisting of triple salt (Potassium monopersulfate) to major aqua pathogens. J. Fish. Pathol., 36(1): 161-170, 2023. http://dx.doi.org/10.7847/jfp.2023.36.1.161
  5. Food and Agriculture Organization of the United Nations (FAO). The state of world fisheries and aquaculture. towards blue transformation; Rome, Italy, 2022.
  6. Griffin, M.J., Ware, C., Quiniou, S.M., Steadman, J.M., Gaunt, P.S., Khoo, L.S. and Soto, E.: Edwardsiella piscicida identified in the southeastern USA by gyrB sequence, species-specific and repetitive sequence-mediated PCR. Dis. Aquat. Org., 108:23-35, 2014. https://doi.org/10.3354/dao02687
  7. Han, H.J., Song, J.Y., Cho, M.Y., Choi, H.S., Jung, S.H. and Seo, H.G.: Monitoring of diseases including nematode Clavinema mariae infections in the cultured Korean rockfish Sebastes schlegelii During 2013-2016. Fish Aquat. Sci., 53(3): 432-442, 2020. https://doi.org/10.5657/KFAS.2020.0432
  8. Han, J.D. and Lee, D.C.: A review of the mass-mortalities of sea-cage farm fishes. J. Fish. Pathol., 35(1): 1-25, 2022. http://dx.doi.org/10.7847/jfp.2022.35.1.001
  9. Im, C.H. and Shin, Y.H.: A comparative study on the classification and detection of disease symptoms using internal organ imaging of Paralichthys olivaceus. J. Korean Inst. Intell., 34(1): 51-57, 2024. http://dx.doi.org/10.5391/JKIIS.2024.34.1.51
  10. Jee, B.Y., Shin, K.W., Lee, D.W., Kim, Y.J. and Lee, M.K.: Monitoring of the mortalities and medications in the inland farms of olive flounder, Paralichthys olivaceus, in South Korea. J. Fish. Pathol., 27(1): 77-83, 2014. https://doi.org/10.7847/jfp.2014.27.1.077
  11. Jee, B.Y., Lee, N.S., Kim, J.W., Park, M.S. and Jeong, S.H.: The preventive effects of hydrolysis compound of cactus opuntia genus, Opuntia ficus-indica var. saboten Makino against bacterial disease on cultured flounder, Paralichthys olivaceus. J. Fish. Pathol., 20(2): 179-187, 2007.
  12. Jeong, Y.U., Kang, C.Y., Kim, M.J., Heo, M.S., Oh, D.C. and Kang, B.J.: Characterization of streptococcosis occurrence and molecular identification of the pathogens of cultured flounder in Jeju Island. Korean J. Microbiol., 42(3): 199-204, 2006.
  13. Jung, S.H, Choi, H.S., Do, J.W., Kim, M.S., Kwon, M. G., Seo, J.S., Hwang, J.Y., Kim, S.R., Cho, Y.R., Kim, J.D., Park, M.A., Jee, B.Y., Cho, M.Y. and Kim, J.W.: Monitoring of bacteria and parasites in cultured olive flounder, black rockfish, red sea bream and shrimp during summer period in Korea from 2007 to 2011. J. Fish. Pathol., 25(3): 231-241, 2012. https://doi.org/10.7847/jfp.2012.25.3.231
  14. Kang, J.Y., Son, H.S. and Choi, H.S.: A Comparison of pre-processing techniques for enhanced identification of Paralichthys olivaceus disease based on deep learning. J. Korea Content. Assoc., 22(3): 71-80, 2022. https://doi.org/10.5392/JKCA.2022.22.03.071
  15. Kim, D.H., Lim, B.S., Ki, J.S. and Park, S.W.: Disease pathogen detection status of the cultured catfish, Silurus asotus in Chungcheong and Jeolla provinces during 2004~2018. J. Fish Mar. Sci. Edu., 32(4): 879-890, 2020. https://doi.org/10.13000/JFMSE.2020.8.32.4.879
  16. Kim, J.W., Lee, H.N., Jee, B.Y., Woo, S.H., Kim, Y.J. and Lee, M.K.: Monitoring of the mortalities in the aquaculture farms of South Korea. J. Fish. Pathol., 25(3): 271-277, 2012. http://dx.doi.org/10.7847/jfp.2012.25.3.271
  17. Kim, K.I., Won, K.M., Lee, E.S., Cho, M.Y., Jung, S.H. and Kim, M.S.: Detection of Vibrio and ten Vibrio species in cage-cultured fish by multiplex polymerase chain reaction using house-keeping genes. Aquaculture 506:417-423, 2019. https://doi.org/10.1016/j.aquaculture.2019.03.073
  18. Kim, Y.C. and Jeong, H.D.: Pathogenicity of new reassortant betanodaviruses to various juvenile fishes. J. Fish. Pathol., 34(2): 133-140, 2021. https://doi.org/10.7847/jfp.2021.34.2.133
  19. Korean Statistical Information Service (KOSIS). Retrieved from http://kosis.kr. 2023.
  20. Lane, D.: 16S/23S rRNA sequencing. In: nucleic acid techniques in bacterial systematics. John Wiley and Sons, Chichester, UK, 1991.
  21. Lee, N.S., Eun, Y.M., Kim, K.I., Choi, H.S., Jung, S.H. and Cho, M.Y.: About streptococcosis of cultured olive flounder, Paralichthys olivaceus in South Korea. J. Mar. Sci., 5(1): 1-8, 2020. https://doi.org/10.23005/KSMLS.2020.5.1.1
  22. Shim, J.D., Hwang, S.D., Jang, S.Y., Kim, T.W. and Jeong, J.M.: Monitoring of the mortalities in oliver flounder (Paralichthys olivaceus) farms of Korea. J. Fish. Pathol., 32(1), 29-35, 2019. https://doi.org/10.7847/jfp.2019.32.1.029
  23. Son, H.S. and Choi, H.S.: Image Augmentation of Paralichthys olivaceus disease using SinGAN deep learning model. J. Korea Content. Assoc., 21(12), 322-330, 2021. https://doi.org/10.5392/JKCA.2021.21.12.322
  24. Woo, S.H., Kim, H.J., Lee, J.S., Kim, J.W. and Park, S.I.: Pathogenicity and classification of streptococci isolated from cultured marine fishes. J. Fish Pathol., 19:17-33, 2006.
  25. Woo, S.J. and Chung, J.K..: Pharmacokinetics of oxy-tetracycline after dipping to cultured rock fish, Sebastes schlegelii. J. Kor. Soc. Fish. Mar. Edu., 30: 2120-2129, 2018. https://doi.org/10.13000/JFMSE.2018.12.30.6.2120
  26. Yoon, M.J., Lee, J.D., Kang, K.h., Park, S.Y. and Kim, J.G.: Physicochemical properties of olive flounder Paralichthys olivaceus, red seabream pagrus major and jacopever Sebastes schlege. J. Kor. Soc. Fish. Mar. Edu., 27(5):1447-1456, 2015. https://doi.org/10.13000/JFMSE.2015.27.5.1447