• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4203-4209
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• 2015

Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased miR-21 levels in BGC-823, but miR-21inhibitor loaded in exosomes exerted an opposite effect. miRNA transfected with exosomes had less cellular toxicity to host cells compared to conventional transfection methods. The miR-21inhibitor loaded exosomes promoted the migration ability and reduced apoptosis of BGC-823 gastric cancer cells. These observations indicate that miR-21 acts as a tumor promoter by targeting the PDCD4 gene and preventing apoptosis of gastric cancer cells through inhibition of PDCD4 expression. Furthermore, exosome -mediated miR-21 inhibitor delivery resulted in functionally more efficient inhibition and less cellular toxicity compared to conventional transfection methods. Similar approaches could be useful in modification of target biomolecules in vitro and in vivo. These findings contribute to our understanding of the functions of miR-21 and exosomes as a carrier for therapy of gastric cancer.

• The Journal of the Korea Contents Association
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• v.11 no.8
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• pp.59-67
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• 2011

Digital Contents have been considered as major parts in the areas, such as design, cartoon, video and etc. A number of Digital Contents are made with the works of popular computer program, Photoshop, and used in some courses of making works. Channel is obviously regarded as a high-level function among others in using Photoshop, even though it hinder a lot of users in understanding the way and the principle to use. Channel and Displacement Map have something in common, because they share the infoshade of channel. The infoshade is the information of 256 levels which consists of black and achromatic colors between white and black color. The infoshade of Channel is used as a concept of movement in Displacement Map. Displacement Map analyzes infoshade in 'Displacement map images' and transfer the pixels of original images under the application of displacement map. This means that the infoshade of Channel can be made for the application with characteristic of movement. This study was carried out to analyze the principle of application of infoshade in 'Displacement Map' with Photoshop. Thus, the central aim of this study is not so much studying engineering algorithm as studying and analyzing the principle of Channel's infoshade through studying how the infoshade of channel is applied in the work.

• The Journal of Korean Medicine
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• v.22 no.4
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• pp.10-21
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• 2001

Objective : To investigate the effect between Fructus Immaturus Ponciri (FIP, the immature fruit of Poncirus trifoliata) and Fructus Ponciri (FP, the ripe fruit of Poncirus trifoliata) on mast cell-mediated immediate-type allergic reactions. Methods : We performed anaphylactic reaction, histamine release, cAMP, $TNF-{\alpha}$, IgE. Results : The aqueous extract of FIP dose-dependently inhibited systemic and local allergic reaction was induced by compound 48/80 or anti-dinitrophenyl (DNP) IgE in a murine model. FIP also significantly inhibited mast cell-dependent ear swelling response induced by topical application of compound 48/80. When mice were orally pretreated with FIP, the plasma histamine levels were reduced in a dose-dependent manner. FIP dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) was activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMCs, when FIP was added, increased compared with that of a normal or control. In addition, FIP had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-a ($TNF-{\alpha}$) production from the RPMCs and IgE produced by lipopolysaccharide-stimulated murine whole spleen cells or U266B1 as human IgE-bearing B cells. However, FP showed the lower inhibition rate than those of FIP in above all allergic reactions. Conclusion : These data have important implications for our understanding of the clinical effects of FIP and FP on allergic diseases, and FIP is more effective than FP on the allergic reaction.

• The Journal of the Korea Contents Association
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• v.16 no.7
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• pp.566-574
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• 2016

The purpose of this study was to determine the viewing trends in eco-childhood education. Analyte is analyzed, such as from 10 March 2008 and 2015 annual target of 97 pieces of thesis until March trends, research content, research methods. As a result, first, the ecological childhood education subjects infant destination, Teachers, took place in indoor and outdoor environments subject, parent targeted net was a study of the most frequent targets 5 years of single age, 4 years old, 3 years old, It was a three-order. Second, the research method consisted of the most experimental studies. Third, research topics include ecology toddler exploring related variables of education, ecological infant was the most common educational programs academic and theoretical basis for the direction sought, ecological Early Childhood Curriculum understanding and national levels of the education process, linking childcare courses, ecology teacher education the program was in order. Fourth, training in experiential learning is the most common forest garden gardening had appeared, walking activities, ecology, art, spirituality, work, raise animals and plants, environmental education in order.

• The Journal of Society for e-Business Studies
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• v.16 no.2
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• pp.39-71
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• 2011

Lately, developing systems fully interoperable with others is considered an essential element for successful projects, as not only do e-commerce becomes ubiquitous but also distributed systems' paradigm spreads. However, since definitions of interoperability vary by viewpoints, it is still difficult to have the same understanding and evaluation criteria on interoperability. For instance, various interoperability parties in military use different definitions of interoperability, and its T&E is not conducted according to the definition, but only to levels of information exchange. In this paper, we proposed a new definition of interoperability as followsm First of all, we collected existing and various interoperability definitions, extracting key components in each of them. Second, we statistically analyzed those components and applied the association rules discovery in data mining. We compared existing interoperability definitions to ours. From this research, we found associations among the components from various definitions applying market-basketanalysis, redefining interoperability. Key findings of this research can contribute to a unified viewpoint on the definition, level, and evaluation items of interoperability.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.3
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• pp.448-457
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• 2002

Heat treatment such as quenching of a high-temperature cylinder is being used on steel to produce high strength levels. Especially, the mist cooling with the high and uniform surface heat flux rate s expected to contribute for better products. The experimental mist cooling curve is produced for better understanding, and two distinct heat transfer regions are recognized from the cooling curve produced. It is shown that the liquid film evaporation dominated region follows the film boiling-dominated region as decreasing the temperature of test cylinder by mist flow. Based on the intuitive view from some previous investigations, a simplified model with some assumptions is introduced to explain the mist cooling curve, and it is shown that the estimation agrees well with our experimental data. In the meanwhile, it is known that the wetting temperature, at which surface heat flux rate is a maximum, increases with mass flow rate ratio of water to air ($\varkappa$ < 10). However, based on our experimental data, it is explained that there exists a critical mass flow rate ratio, at which the wetting temperature is maximum, in the range of 3 < $\varkappa$ < 130. Also, it is described that despite of the same value of $\varkappa$, the wetting temperature may increase with mist velocity.

• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.78-78
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• 2009

$^1H$ NMR spectroscopy coupled with multivariate statistical analysis was used for the first time to investigate metabolic changes in musts during alcoholic fermentation and wines during ageing. Three Saccharomyces cerevisiae yeast strains (RC-212, KIV-1116 and KUBY-501) were also evaluated for their impacts on the metabolic changes in must and wine. Pattern recognition (PR) methods, including PCA, PLS-DA and OPLS-DA scores plots, showed clear differences for metabolites among musts or wines for each fermentation stage up to 6 months. Metabolites responsible for the differentiation were identified to valine, 2,3-butanediol (2,3-BD), pyruvate, succinate, proline, citrate, glycerol, malate, tartarate, glucose, N-methylnicotinic acid (NMNA), and polyphenol compounds. PCA scores plots showed continuous movements away from days 1 to 8 in all musts for all yeast strains, indicating continuous and active fermentation. During alcoholic fermentation, highest levels of 2,3-BD, succinate and glycerol were found in musts with the KIV-1116 strain, which showed the fastest fermentation or highest fermentative activity of the 3 strains, whereas the KUBY-501 strain showed the slowest fermentative activity. This study highlights the applicability of NMR-based metabolomics for monitoring wine fermentation and evaluating the fermentative characteristics of yeast strains.

• KIEAE Journal
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• v.7 no.1
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• pp.15-22
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• 2007

Indoor air quality(IAQ) problems are not limited to large office buildings that are inadequately operated. In fact, many school buildings have significant potentials of air pollution and indoor air pollutants may be of particular concern because it is estimated that most young students who are physically weak to the pollutants spend about 90% of their time in classrooms. The goal of the study is to provide academic and architectural information on the major factors that influence indoor air quality in the elementary school. In order to gain a better understanding of IAQ problems in schools, a series of measurement studies of indoor air quality were designed and 3 different elementary school buildings located in Chuncheon were selected. The levels of CO, $CO_2$, and total volatile organic compounds(TVOCs) including formaldehyde(HCHO), major concern of this study, were measured in absence of students after class. As the results, it is noticeable that most indoor air pollution comes from sources inside the building, which are, for example, adhesives, upholstery, manufactured wood products, art and scientific supplies. The level of CO was measured similarly to that of outdoors, which means no impact on the indoor air problems since it is lower than the code. Ventilation played an important role in the level of $CO_2$ of which difference was 1.7 times in rough and 230 % of difference in the level was detected among the 3 school buildings. This concludes that indoor air problems might be result of poor building design with inadequate location of corridors within space organization or occupant activity patterns of ventilation.

• Journal of Microbiology and Biotechnology
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• v.28 no.10
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• pp.1736-1748
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• 2018

Brucella abortus can survive and replicate within host macrophages, and great efforts have been made to demonstrate the genes involved in pathogenicity, such as internalization, in Brucella research. Here, intracellular responses were compared between THP-1 macrophage cells stimulated with B. abortus wild-type and four mutants (C1, C10, C27, and C32) using microarray to demonstrate the role of genes related to intracellular survival and replication. These mutants were generated by deleting genes encoding BAB_RS13225 (4-hydrobenzoate 3-monooxygenase, PHBH), BAB_RS00455 (heme exporter protein cytochrome C, CcmC), BAB_RS03675 (exopolyphosphatase, PPX), and BAB_RS13225 (peptidase M24). The results showed that mutants C1 and C10 induced significant suppression of survival levels and cytokine expression relative to wild-type in the THP-1 macrophage cells. These findings suggest that the BAB_RS13225 and BAB_RS00455 genes play important roles in survival within human macrophages. Conversely, mutants C27 and C32 induced significantly higher survival level than wild-type in the cells inhibiting cellular signal transduction. It is assumed that the BAB_RS03675 and BAB_RS13225 genes play a role in cellular resistance to B. abortus. Therefore, the disrupted genes are involved in B. abortus intracellular growth, and especially in its survival, and they could be effective targets for understanding the intracellular bacterium, B. abortus.

• Journal of the Korean Society for information Management
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• v.20 no.4 s.50
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• pp.135-158
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• 2003

The Purpose of this study is to investigate how information use contributes to learning. Conducted as part of a larger study, this study focuses on learning by analyzing students' use of cognitive skills during the Process of using information. Within the broad methodological framework of qualitative research in constructivist paradigm (Guba and Lincoln, 1998), the study applied the revised Bloom's taxonomy (Anderson and Krathwohl, 2000) as a particular framework to understand the Phenomenon. Participants included 21 high school juniors in an honors' class of persuasive speech. The study's combinational use of two techniques -concept mapping and individual interview - in a naturalistic setting Proved to be the unique methods for researching the reflection of information use in learning Products. The results revealed that changes in students' understanding occured in four types - simple, analytic, organizational, and holistic changes. The analysis using the revised Bloom's taxonomy showed that a variety of cognitive skills were used during the whole process of information use and that the use of higher levels of cognitive skills is particularly crucial.