• Title/Summary/Keyword: uDA

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Induction of Pacemaker Currents by DA-9701, a Prokinetic Agent, in Interstitial Cells of Cajal from Murine Small Intestine

  • Choi, Seok;Choi, Jeong June;Jun, Jae Yeoul;Koh, Jae Woong;Kim, Sang Hun;Kim, Dong Hee;Pyo, Myoung-Yun;Choi, Sangzin;Son, Jin Pub;Lee, Inki;Son, Miwon;Jin, Mirim
    • Molecules and Cells
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    • v.27 no.3
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    • pp.307-312
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    • 2009
  • The interstitial cells of Cajal (ICC) are pacemaking cells required for gastrointestinal motility. The possibility of whether DA-9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber, modulates pacemaker activities in the ICC was tested using the whole cell patch clamp technique. DA-9701 produced membrane depolarization and increased tonic inward pacemaker currents in the voltage-clamp mode. The application of flufenamic acid, a non-selective cation channel blocker, but not niflumic acid, abolished the generation of pacemaker currents induced by DA-9701. Pretreatment with a $Ca^{2+}$-free solution and thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum, abolished the generation of pacemaker currents. In addition, the tonic inward currents were inhibited by U-73122, an active phospholipase C inhibitor, but not by $GDP-{\beta}-S$, which permanently binds G-binding proteins. Furthermore, the protein kinase C inhibitors, chelerythrine and calphostin C, did not block the DA-9701-induced pacemaker currents. These results suggest that DA-9701 might affect gastrointestinal motility by the modulation of pacemaker activity in the ICC, and the activation is associated with the non-selective cationic channels via external $Ca^{2+}$ influx, phospholipase C activation, and $Ca^{2+}$ release from internal storage in a G protein-independent and protein kinase C-independent manner.

데데킨트 절단, 배중률, 관계

  • Hong, Seong-Gi
    • Korean Journal of Logic
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    • v.7 no.2
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    • pp.15-46
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    • 2004
  • Um die rationalen Zahlen auf die reellen Zahlen zu erweitern und dadurch die Stetigkeit der reellen Zahlen sicherzustellen, hat der deutsche Mathematiker R. Dedekind im Jahr 1872 in seinem Aufsatz "Stetigkeit und Irrationale Zahlen" einen neuen mathmatischen Begriff $eingef\ddot{u}hrt,\;n\ddot{a}mlich$ 'Schnitt'. Die Menge aller rationalen Zahlen Q wird durch eine rationale Zahl a zu zwei Untermengen $A_1=\{x|x{\leq}a,\;x{\in}Q\}$, $A_2=\{x|x>a,\;x{\in}Q\}$ $vollst\ddot{a}ndig$ geteilt. Wenn wir solche Teilung, d.i. solchen Schnitt mit "$(A_1,\;A_2)$" bezeichnen, ist ein $Identit\ddot{a}tssatz$ "a=$(A_1,\;A_2)$" absolut harmlos. Analog dazu glaubt Dedekind fest, $da{\beta}$ jede irrationale Zahl mit Hilfe von einem entsprechenden Schnitt $einzuf\ddot{u}hren$ ist. Zum Beispiel, falls die zwei Mengen $B_1=\{x|x^2<2,\;x{\in}Q\}$ und $B_2=\{x|x^2>2,\;x{\in}Q\}$ gegeben sind, dann $w\ddot{a}re$ die irrationale Zahl $^{\surd}2$ mit $(B_1,\;B_2)$ gleichzusetzen. Im Fall von einem Schnitt der Menge der rationalen Zahlen durch eine rationale Zahl, $(A_1,\;A_2)$, haben die beiden Untermengen $A_1$ und $A_2$ jwewils ein Supremum und ein Infimum und beide $m\ddot{u}ssen$ identisch sein, aber -wie schon Russell in seinem Buch "Introduction to Mathmatical Philosophy" dies kritisiert- hat ein Schnitt $f\ddot{u}r$ die $Einf\ddot{u}hrung$ der irrationalen Zahl, $(B_1,\;B_2)$ keine solche $gl\ddot{u}cklichen$ Eigenschaften. Dennoch glaubt Dedekind an eine streng wissenschaftliche Fundierung der irrationalen Zahl fest, und $w\ddot{u}rde$ nach dem Grund seines Glaubens befragt, $k\ddot{o}nnte$ er nur seine Behauptung wiederholen, ein klarer Fall circulus vitiosus. Mit anderen Worten, die $L\ddot{u}cke$ zwischen $B_1$ und $B_2$ durch die $Einf\ddot{u}hrung$ einer [einzigen] wissenschaftlich fundierten irrationalen Zahl $\ddot{u}berbr\ddot{u}ckt$ und das Ganze zu einem Kontinium gemacht werden sollte, bleibt dieses Vorhaben von Dedekind erst als eine Hoffnung und dessen Resultat kann $h\ddot{o}chstens$ nur als ein Postulat, aber keineswegs als ein methodisch einwandfreier Beweis betrachtet werden. Die Probleme, die mit dem Versuch der $Einf\ddot{u}hrung$ der irrationalen Zahlen mit Hilfe von Schnitt verbunden sind, sind nicht spezifisch allein im Gebient der Mathmatik, sondern betreffen immer wieder die Rechtfertigungsfrage der $Einf\ddot{u}hrung$ der letzten Bestandteile im bezug auf eine Systemerstellung, egal ob dies System ein Wissenschaftliches oder unsere $allt\ddot{a}gliche$ Sprachhandlung ist. $F\ddot{u}r$ all diese Rechtfertigungsfragen gilt das in der klassischen Logik $g\ddot{a}ngige$ logische Prinzip tertium non datur nicht mehr, aber nicht nur wegen der von praktischen $Unm\ddot{o}glichkeit$, die unendlichen vielen $Gegenst\ddot{a}nde$ durchforschen zu $m\ddot{u}ssen$, das $hei{\beta}t$, wegen der erkenntnistheoretischen $Beschr\ddot{a}nktheit$ des jetzigen Erkenntnisniveau, sondern auch wegen des speziellen ontologischen modus der $eizuf\ddot{u}hrenden$ Objekten. Der Autor des Aufsatzes analysiert $\ddot{a}nliche$ $F\ddot{a}lle$ (das Urmeterbeispiel und die Chrakterisierungen der geometrischen Axiome von Wittgenstein), und versucht mit Hilfe von beiden Begriffen, 'interne' und 'externe' Relation, zu zeigen, $da\beta$ eine gemeinsame, invariante Struktur in den eben genannten $F\ddot{a}llen$ besteht. Am Ende des Aufsatzes setzt der Autor sich mit der logischen Argumentationsstruktur des Zitates tiber 'Grenze' aus Noten von Leonardo da Vinci auseinander, und weist auf einen $m\ddot{o}glichen$ Zusammenhang der Grundidee seienes Aufsatzes mit der Philosophie der indischen Denker $N\bar{a}g\bar{a}rjuna$ hin, obwohl die zitierten Versen aus dem Hauptwerk von $N\bar{a}g\bar{a}rjuna$, dem Mittleren Weg$(Madhyamakak\bar{a}rik\bar{a})$ nur andeutend sein $m\ddot{o}gen$.

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Identification of Be Levels Correlated with Intrinsic Defect in p-GaSb Grown by Molecular Beam Epitaxy

  • Kim, Jun-O;Lee, Sang-Jun;Kim, Chang-Su;No, Sam-Gyu;Choe, Jeong-U;Park, Dong-U;Kim, Jin-Su;Kim, Jong-Su
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.167-167
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    • 2010
  • 반도체는 도핑하지 않으면 대부분 n형을 나타내는 것에 반하여 GaSb는 p형을 보이는 반도체로서, 그 근원은 명확하게 규명되어 있지 않은 상태이다. GaSb의 p형 불순물인 Be은 Ga과 치환 ([$Be_{Ga}$])되므로, p형 전도의 근원으로 추정되는 잔존결함인 [$Ga_{Sb}$]와 그 복합체인 [$Ga_{Sb}-Sb_{Ga}$]와 높은 상관관계를 가질 것으로 예측된다. 본 연구에서는 Be을 도핑한 GaSb:Be 에피층을 MBE 방법으로 성장하여, PL 스펙트럼과 Hall 효과 분석을 통하여 p형 전도의 근원을 조사하였다. 도핑하지 않은 u-GaSb는 DA (deep acceptor)와 함께 A 준위를 나타낸 반면, p-GaSb:Be의 PL 스펙트럼은 Be 도핑농도가 증가함에 따라 FWHM가 줄어들면서 점차 높은 에너지 영역으로 변위하지만 농도가 가장 높은 시료에서는 PL의 FWHM가 증가하면서 에너지는 감소함이 관측되었는데, 이것은 A 피크와 Sb 관련 피크가 경쟁적으로 중첩되어 나타난 현상으로 분석된다. Hall 효과 결과는 유효 전하밀도의 증가에 따라 이동도는 감소하는 전형적인 의존성을 나타내었으며, u-GaSb의 Hall 이동도가 p-GaSb:Be의 값보다 작은 것은 u-GaSb에 잔존하는 DA에 의한 산란 때문으로 해석된다. Gaussian 형태로 분해하여 얻은 A ([$Ga_{Sb}$])와 DA ([$Ga_{Sb}-Sb_{Ga}$]) 및 Be 관련 피크로부터 특정 도핑농도 ($1.2{\times}10^{17}cm^{-3}$)의 시료를 제외한 모든 p-GaSb:Be에는 A 피크가 중첩되고 A와 Be 준위 중간에 Be과의 복합체인 중간상태(intermediate state)인 [$Be^*$]가 존재함이 관측되었는데, 특정 도핑농도에서는 [$Be_{Ga}$]이 우세하지만 더 이상 농도가 증가하면 [$Be_{Ga}$] 준위의 강도는 오히려 감소함을 관측할 수 있었다. 이것은 적정 이상의 Be을 도핑할 경우, A ([$Be_{Ga}$])와 $Be^*([Be_{Ga}-Ga_Sb}])$가 형성 ($A[Ga_{Sb}]+Be{\rightarrow}Be^*[Be_{Ga}-Ga_{Sb}]+[Be_{Ga}]$)됨을 보여 주는 중요한 결과인 것으로 분석된다. A, [Be], [$Be^*$] PL 피크 에너지는 각각 779, 787, 794 meV (오차범위 ${\pm}3\;meV$)이고, [$Be_{Ga}$]의 활성화 에너지는 ($23{\pm}3\;meV$) (20 K)임을 밝혔다.

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PostScript Conversion of ODIF Data Stream (ODIF 데이터스림의 포스트스크립트 변환)

  • 홍온선;윤근종;이수연
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.16 no.11
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    • pp.1027-1036
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    • 1991
  • This paper proposes an implementation of ODIF(Open Document Interchange Format)decoder and PostScript converter. AS ODIF data stream based on IS 8613 is described according to ASN.1 notation it is necessary to decode ODIF data stream to the proper internal structure to PostScript format as proposed in order to make hard copies in good quality using LBP(Laser Beam Printer). Among several kinds of DA(Document Architecture) and DAP(Document Application Profile). PDA(Processable DA) and Core 26(Level 2 DAP) are selected for our study. An ODIF data stream submitted by ICL is used to show the conformance in the level of data stream.

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Molecular Mechanism of Copper Resistance in Pseudomonas syringae pv. tomato.

  • Cha, Jae-Soon;Donald A. Cooksey
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1995.06b
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    • pp.97-117
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    • 1995
  • Copper resistance in Pseudomonas syringae pv. tomato is determined by copper-resistance operon (cop) on a highly conserved 35 kilobase plasmid. Copper-resistant strains of Pseudomonas syringae containing the cop operon accumulate copper and develop blue clonies on copper-containing media. The protein products of the copper-resistance operon were characterized to provide an understanding of the copper-resistance mechanism and its relationship to copper accumulation. The Cop proteins CopA (72 kDa), CopB (39 kDa), and CopC (12 kDa) were produced only under copper induction. CopA and CopC were periplasmic proteins and CopB was an outer membrane protein. Leader peptide sequences of CopA, CopB, and CopC were confirmed by amino-terminal peptide sequencing. CopA, CopB, and CopC were purified from strain PT23.2, and their copper contents were determined. One molecule of CopA bound 10.9${\pm}$1.2 atoms of copper and one molecule of CopC bound 0.6${\pm}$0.1 atom of copper. P. syringae cells containing copCD or copBCD cloned behind the lac promoter were hypersensitive to copper. The CopD (32 kDa), a probable inner membrane protein, function in copper uptake with CopC. The Cop proteins apparently mediate sequestration of copper outside of the cytoplasm as a copper-resistance mechanism.

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A New Link-Based Single Tree Building Algorithm for Shortest Path Searching in an Urban Road Transportation Network

  • Suhng, Byung Munn;Lee, Wangheon
    • Journal of Electrical Engineering and Technology
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    • v.8 no.4
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    • pp.889-898
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    • 2013
  • The shortest-path searching algorithm must not only find a global solution to the destination, but also solve a turn penalty problem (TPP) in an urban road transportation network (URTN). Although the Dijkstra algorithm (DA) as a representative node-based algorithm secures a global solution to the shortest path search (SPS) in the URTN by visiting all the possible paths to the destination, the DA does not solve the TPP and the slow execution speed problem (SEP) because it must search for the temporary minimum cost node. Potts and Oliver solved the TPP by modifying the visiting unit from a node to the link type of a tree-building algorithm like the DA. The Multi Tree Building Algorithm (MTBA), classified as a representative Link Based Algorithm (LBA), does not extricate the SEP because the MTBA must search many of the origin and destination links as well as the candidate links in order to find the SPS. In this paper, we propose a new Link-Based Single Tree Building Algorithm in order to reduce the SEP of the MTBA by applying the breaking rule to the LBA and also prove its usefulness by comparing the proposed with other algorithms such as the node-based DA and the link-based MTBA for the error rates and execution speeds.

PROTECTIVE EFFECT OF DA-3711, A NEW ANTIWRINKLE AGENT, AGAINST UVB-INDUCED SKIN DAMAGE IN HAIRLESS MICE MODEL

  • Kim, J. M.;Y. S. Sohn;K. K. Kang;Lee, E. D.;Lee, M. Y.;B. O. Ahn;Lee, J. H.;J. W. Kwon;Kim, W. B.
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.698-699
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    • 2003
  • DA-3711 is a novel, anti wrinkle agent containing growth factors derived from human skin culture. In this study, effect of topical application of DA-3711 on chronic ultraviolet B (UVB)-induced skin damage was evaluated in hairless mice model. Exposure to UVB for 8 weeks induced apparent wrinkles on the back skin of the mice. The dorsal surfaces were exposed to UVB for a further 8 weeks, during which the surfaces where treated daily by topically application of a lotion containing either 35% or 70% of DA-3711. For comparison, lotion base, lotion base containing Cylasphere Retinol(equation omitted) (2500 I.U., Coletica) and NouriCe $l^{(R)}$ (Biozhem) were applied topically once a day for 8 weeks.(omitted))

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Purification and Biochemical Characteristics of a 45 kDa Fibrinolytic Enzyme from a Halophile (호염성균 유래 45 kDa 혈전용해효소의 순수분리와 생화학적 특성)

  • Kim, Do-Hyoung;Park, Jeong-Uck;Seo, Min-Jeong;Kim, Min-Jeong;Lee, Hye-Hyeon;Choi, Yung-Hyun;Joo, Woo-Hong;Jeong, Yong-Kee
    • Journal of Life Science
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    • v.20 no.2
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    • pp.183-189
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    • 2010
  • A fibrinolytic enzyme producing Bacillus sp. J-19 was isolated from the popular Korean seasoning, pickled anchovy. The fibrinolytic enzyme was purified to homogeneity by chromatographic methods including ethanol precipitation and gel-filtration using Sephadex G-50. Compared to the crude enzyme extract, the specific activity of the enzyme increased 1021-fold with a recovery of 23%. The purified enzyme was estimated to be approximately 45 kDa by SDS-PAGE. Especially, the amidolytic activity in the presence of the synthetic substrate for serine protease (H-D-Ile-Pro-Arg-pNA, S-2288) represented approximately 17 U/mg. In addition, more than the 60% activity of the 45 kDa fibrinolytic activity was maintained in the presence of up to 30% (w/v) sodium chloride. These findings could provide a unique fibrinolytic enzyme, leading to a potential thrombolytic agent.

Der anti-theatralische Topos im modernen Theater -Die Ästhetik der Abwesenheit und des Webens- (현대 연극의 반-연극적 지형 -부재와 직조의 미학-)

  • Lee, Kyung-Mi
    • Journal of Korean Theatre Studies Association
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    • no.40
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    • pp.277-305
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    • 2010
  • Die heutigen Experimente, die seit fast 20 Jahren in den Theaterbereiche $durchgef{\ddot{u}}hrt$ werden, sind so verschieden, $da{\ss}$ wir gar nicht voraussehen $k{\ddot{o}}nnen$, wieweit sie ihre Grenze mehr erweitern werden. Sie brechen alle vertrauten theatralischen Konventionen, ob diese Konventionen mit Text, Schauspielkunst oder Raum usw. zu tun haben. Und sie fragen sich selbst, was Theater in der heutigen simulierten Gesellschaft bedeuten soll. Die vorliegende Arbeit untersucht anhand einigen $Auff{\ddot{u}}hrungen$, die von solchen Selbstbefragen ausgehen, wieweit sie sich von den tradtionellen Theaterabeiten entfernen und welche neue $Theater{\ddot{a}}sthetik$ sie herzustellen versuchen, um der $gegenw{\ddot{a}}rtigen$ Gesellschaft, besonders nach 1990 Jahre, zu entsprechen. Nach dem Zusammenbruch des Ost-Blocks hat sich die Welt radikaler denn je $ver{\ddot{a}}ndert$. Durch die $revolution{\ddot{a}}ren$ Technologien wie Mobilfunk und Internet verwischen sich die Grenzen zwischen Wirklichkeit und Fiktion, zwischen Original und Nicht-original mehr und mehr. Die letztliche globale Finanz- und Wirtschaftskriese hat die ganze Welt in Schockzustand versetzt, aber man scheint bei den verschiedenen Schreckenzeneraien und den negativen Meldungen daraus noch keine "Lehre" gezieht zu haben. Die Experimente, die in dem Bereich der zeitigen Theaterarbeiten gewagt werden, sind die Versuche, die Theater selbst macht, um eine den radikalen gesellschaflichen $Ver{\ddot{a}}nderungen$ entsprechende neue $k{\ddot{u}}nstliche$ Aufgabe zu suchen. In dieser Arbeit $m{\ddot{o}}chte$ ich sie, $ant{\acute{i}}-theatralische$ Strategien nennen, weil sie jenseits der konventionellen theatralischen Arbeitsweise liegen. Diese $R{\ddot{a}}ume$, wo solche $Auff{\ddot{u}}hrungen$ gespielt werden und $nat{\ddot{u}}rlich$ auch den Zuschauerraum $einschlie{\ss}en$, sind, die $R{\ddot{a}}ume$ des Abwesenheit und des Abweichens,,, weil sie dem $gew{\ddot{o}}hnlichen$ Wunsch des Publikums, alles zu sehen, entgegenkommen. Sie fordern dem Zuschauer, der auch zu ihren wichtigen theatralische Element $geh{\ddot{o}}rt$, $da{\ss}$ er an ihren $Auff{\ddot{u}}hrungen$ mit den ganz anderen Sichtweise als den ${\ddot{u}}berlieferten$ aktiv, teilnehmen, $mu{\ss}$. Sie wollen ihre $Auff{\ddot{u}}hrungen$ zu den $Erfahrungsr{\ddot{a}}umen$ machen, wo der Zuschauer ${\ddot{u}}ber$ sich und sein eigenes Leben selbst reflektieren kann, indem er die wahrgenommenen Ereignisse zu seinen eigenen Erfahrungen $zusammenf{\ddot{u}}gt$. Dadurch wird die Dichtonomie zwischen den Sichtbaren und den Nicht-Sichtbaren $entsch{\ddot{a}}rft$.

Induction of Thioredoxin by Oxidative Stress and Overexpression of Thioredoxin in Lung Cancer Tissue (산화 스트레스에 의한 Thioredoxin의 발현과 폐암조직에서의 발현)

  • Lee, Jang-Hoon;Kim, Hyung-Jung;Ahn, Chul-Min;Kim, Sung-Kyu;Lee, Won-Young
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.3
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    • pp.327-337
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    • 1999
  • Background: Reactive oxygen species are involved in multi-stage process of carcinogenesis. The moot of cancer cell lines and cancer cells in tumor tissue produce reactive oxygen species and on the other hand, the activities of catalase, Mn- and CuZn-superoxide dismutase in tumor cells are usually low. These persistent oxidative stress in tumor tissue facilitates tumor invasion and metastasis. 12-kDa thioredoxin, which regulates the intracellular redox potential with glutathione and glutaredoxin is involved in cell activation, proliferation, differentiation and redox-mediated apoptosis. It is also purified as 14-kDa and 10-kDa eooinophilic cytotoxic enhancing factor(ECEF) from human histiocytic cell(U937) and 10-kDa ECEF has more than 20 times eosinophilic stimulation activity than 14-kDa ECEF. It has been reported that adult T-cell leukemia, squamous cell carcinoma of uterine cervix, and hepatocellular carcinoma show increased amounts of human thioredoxin and thioredoxin mRNA is increased in lung cancer. In this study, we investigated the expression of conventional antioxidant enzymes such as catalase, CuZn-SOD, and glutathione peroxidase and thioredoxin in lung cancer tissue compared to adjacent normal lung tissue and the induction of thioredoxin in macrophage cells after treatment of oxidative stress and endotoxin Methods: We measured the amount of conventional antioxidant enzymes such as catalase, CuZn-SOD, and glutathione peroxidase and thioredoxin in lung cancer tissue compared to adjacent normal lung tissue by immunoblot analysis and the induction of thioredoxin in mouse monocyte-macrophage cells(RAW 264.7) by treatment of 5 ${\mu}M$ menadione and 1 ${\mu}g/ml$ endotoxin Results: On immunoblot analysis, the expression of 12-kDa thioredoxin was increased in lung cancer tissue compared to paired normal lung tissue. but the expression of catalase and CuZn-SOD were decreased in lung cancer tissue compared to paired normal tissue and the expression of glutathione peroxidase in lung cancer was variable. The expression of truncated thioredoxin was also increased in lung cancer. When mouse monocyte-macrophage cells were treated with 5 ${\mu}M$ menadione and 1 ${\mu}g/ml$ endotoxin, the expression of thioredoxin was peaked at 12 hrs and sustained to 48 hrs. Conclusion: In contrast with other conventional antioxidants, the expression of 12-kDa and truncated thioredoxin in lung cancer were increased and it is closely associated with persistent oxidative stress in tumor microenvironment. Considering especially the biological functions of truncated thioredoxin, the increased amount of truncated thioredoxin has significant role in tumor growth through cell proliferation.

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