• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.339-345
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• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• Journal of Forest and Environmental Science
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• v.30 no.2
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• pp.243-252
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• 2014

Forest tree species usually takes for long periods to be harvested and cultivated but spearmints are a good model system for woody plant because of reducing and shortening cultivation time. Spearmints are good model plants (Mentha species) for research about terpenoids production and industrial essential oil manufacture. Isopentenyl pyrophosphate isomerase (Iso) and limonene synthase (Limo) are the key enzymes of terpenoid biosynthesis pathway. Transgenic and wild spearmints (Mentha spicata, MS) were cultured in vitro and assessed for the essential oil contents. The content of essential oil of transgenic spearmint also was enhanced slightly depending on the target terpenoid genes. In an attempt to increase productivity of terpenoids further, salt and fungal elicitation strategy was adopted on transgenic Mentha spicata. The salt (800 mM NaCl) as abiotic and two fungi (Botrytis cinerea and Glomerella cingulata) as biotic were used for elicitors. In the absence of salt stress four terpenoids were detected from the spearmint extracts, all of them being monoterpenes. On the other hand, the transgenic (MSIso) extracts contained eleven terpenoids (10 monoterpenes and 1 phenylpropene) while transgenic (MSLimo) extracts contained seven monoterpenes. After 3 days of fungal infection, the resistance indices further increased to 4.38, 3.89 and 2.04 for wild type, MSIso and MSLimo, respectively. The salt and fungal elicitators proved beneficial towards modifying both the terpenoids profile and improvement in the composition of essential oil. These results have important applications for the large-scale production of essential oils and forest biotechnology with respect to spearmint.

• Journal of the Korean Institute of Landscape Architecture
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• v.27 no.3
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• pp.1-17
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• 1999

The purpose of this study is to evaluate the creation techniques of artificial wetland, one of biotopes developed to promote biodiversity in urban areas, and to look for improvement steps. Specifically, artificial wetland creation techniques were categorized into living environment and living creature classification. Being living conditions for creations, habitat environment was reviewed with a focus on water and soil environments. Living creatures were classified into plants, insects, fish, and birds. The evaluation of creation techniques was done in post-construction evaluation while considering the creation of habitats for living creatures. Intervention by users, changes in living environment and living species, and relevance of creation techniques were reviewed. Key results of this study are as follows. (1) Water environment for the living environment of creatures provides a suitable environment conditions for the living of creatures through a process easing the use of piped water. Various water depths and embankment appear to have a positive impact on the living of aquatic life. In particular, embankment covered in soil naturally played an important role as a place for the activities of aquatic insects and young fish as well as the growth of aquatic plants. (2) Various aquatic and ground plants to promote insect-diversity, shallow water, and old-tree logs had contributed greatly in increasing the types and number of insects. Aquatic insects. Aquatic insects were seen much particularly in areas where aquatic plants are rich but water is shallow than any other areas. (3) A space piled with stone to provide habitats for fish was not much used. However, it was observed that fish used embankment built with natural stones and embankment using logs in areas where water is deep. In addition, it was confirmed that 1,500 fish that had been released propagated using various depths and places for birth. (4) It was analyzed that techniques (creation of island, log setting, and creation of man-made bird nests) to provide habitats and to attract birds are not serving their roles. In such a case, it is believed that species had not increased due to the smallness as well as isolated features of the area. Based on theoretical review, they are judged to be areas that are likely to be used when a greater variety of birds is introduced. It is judged that attracting and keeping more birds at the site, such spaces need to be linked systematically in the future in terms of building eco-network while ensuring an adequate living areas. (5) In the study areas, users intervened greatly. As a result, a blockage was created preventing the normal growth of plants and non-indigenous plants were introduced. In order to limit the intervention by users, setting enough buffer zones, and environment education programs were urgently required. D/H=1>Hyangkyo> houses on the river>temples>lecture halls. D/H ratio of the backside areas is as follows. D/H=1>Hyangkyo>houses on the river>lecture halls. 4. Inner garden were planted deciduous than evergreen trees with Lagerstroemia indica. Enclosed dominant trees were planted by Pinus densiflora, Querces seuata. construct GEM strain, and examined for the expression and functional stability in microcosms.

• Journal of the Korean Data and Information Science Society
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• v.22 no.3
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• pp.515-521
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• 2011

There are increasing demands for the producing and breeding new domestic riding horses for the vitalizations of horse riding industry in Korea, according as 'Horse Industry Support Act' became. In this study, we were to develop the functional relation through the conformation comparison & body composition analysis. 76 heads of 5 breeds utilized for riding horses in Korea were used and their body measurements on 12 items were measured and cluster analysis was conducted to determine the correlation relation among them. The measurements were standardized that (height, croup height, pelvis length), and (hip width, width of pelvis) were highly correlated. In these results of the decision tree, we confirmed to classify the breed type determination by their body measurements (hip height, hip width, head length, croup height). This result can be used as basic data for the development of horse type determination (racing, riding, Riding for the Disabled, Working, or fattening) through the analysis of body composition, and be utilized as the basic data for the producing and breeding new domestic riding horses through the 3D Stereosocpic image system analyze.

• Journal of Environmental Impact Assessment
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• v.21 no.6
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• pp.823-839
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• 2012

Environmental Specimen Banks (ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystem based on the retrospective analysis of the representative samples collected regularly and stored in cryogenic condition. In Korea, National Environmental Specimen Bank (NESB) was established in 2009 and the standard operating procedures (SOPs) for sampling, and cryogenic milling and storage had been prepared during 2007-2010. Since then, the tentative SOPs for the seven kinds of specimens (shoots of Red Pine (Pinus densiflora) and Korean Pine (Pinus koraiensis), leaves of Mongolian Oak (Quercus mongolica) and Zelkova Tree (Zelkova serrata), eggs of Feral Pigeon (Columba livia var. domestica), muscles and organs of Common Carp (Cyprinus carpio), and Freshwater Bivalve (Unio (Nodularia) douglasiae)) have been put to test in the field and laboratory as well against the practicality and feasibility. The SOPs were improved by reflecting the findings from the research and the following discussion regarding the selection of specimen (Feral Pigeon suffering from a control management), sample size (a problem of decreasing number of sampling trees related to increasing sampling time) and period (a problem related to a bud growth), and sampling methods etc.. In addition, barcoding system for the management of the specimen information, and monitoring system of the cryogenic storage to regulate the optimum temperature and the liquid nitrogen level were also developed for the efficient and effective control of the samples. Lastly, the safety guide and emergency protocol were augmented to guarantee a safe work environment with the cryogenic facility. These improvements of the SOPs are expected to contribute to more stable operation of the NESB.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.4
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• pp.83-97
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• 2013

The traditional village forests in Korea such as Dangsan forests and Bibo forests form unique cultural landscape and they are national cultural assets with histories of more than several hundred years. It is important to have sustainable right to enjoy cultural heritage as well as maintaining its authenticity. Cultural assets are meaningful only when they are managed to succeed to next generations. Dangsan forests and Bibo forests need to be named properly and recognized by society as a precious cultural heritage for many generations to come in order to claim the value as traditional cultural landscapes in Korea. The traditional village forest in Dangin-ri, Wando-gun was composed of a Dangsan forest and five Bibo forests. Although this traditional village forest is large in size, it was not on the report of Cultural Heritage Administration(2005) investigated for 'Village forest of island region in Jeollanam-do'. The objective of this study was to investigate the characteristics of the traditional village forest in Dangin-ri to grant correct name and to enhance its value. In this study the characteristics of a Dangsan forest and five Bibo forests were investigated from 2006 to 2013 for physical features such as size, shape, location, tree species composition, and diameter at breast height(DBH), as well as cultural aspects and tradition. Currently the naming and designation of natural monuments by the cultural heritage administration(CHA) is based on the rule enacted in 1934. Many Dangsan forests were named as evergreen forests. This type of names does not represent the meaning of traditional village forest. It was suggested that this traditional village forest is to be named as 'Dangsan Forest and Bibo Forests at Dangin-ri, Wando-gun'. This traditional village forest needs to reclaim authenticity and rebuild its original appearance by restoration of the disturbed areas, especially in Bibo forest I.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.37 no.4
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• pp.60-70
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• 2000

An 8$\times$8+20-bit MAC is designed with low power design methodologies at each of the system design levels. At algorithm level, a new method for multipl $y_tract operation is proposed, and it saves the transistor counts over conventional methods in hardware realization. A new Booth selector circuit using NMOS pass-transistor logic is also proposed at circuit level. It is superior to other circuits designed by CMOS in power-delay-product. And at architecture level, we adopted an ELM adder that is known to be the most efficient in power consumption, operating frequency, area and design regularity as the final adder. For registers, dynamic CMOS single-edge triggered flip-flops are used because they need less transistors per bit. To increase the operating frequency 2-stage pipeline architecture is adopted, and fast 4：2 compressors are applied in Wallace tree block. As a simulation result, the designed MAC in 0.6${\mu}{\textrm}{m}$ 1-poly 3-metal CMOS process is operated at 200MHz, 3.3V and consumed 35㎽ of power in multiply operation, and operated at 100MHz consuming 29㎽ in MAC operations, respectively.ly.

• Journal of Broadcast Engineering
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• v.18 no.2
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• pp.271-282
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• 2013

Recently, ITU-T/VCEG and ISO/IEC MPEG have started a new joint standardization activity on video coding, called High Efficiency Video Coding (HEVC). This new standard gives significant improvement in terms of picture quality for high resolution video. The main challenge in this upcoming standard is the time complexity. In this paper we have focused on CU splitting algorithm. We have proposed a novel algorithm which can terminate the CU splitting process early based on the RD cost of the parent and current level and the motion vector value of the current CU. Experimental result shows that our proposed algorithm gives on average more than about 10% decrement in time over ECU [8] with on average 1.78% of BD loss on the original.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.531-539
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• 1994

We examined the in vitro rooting and survival of tissue cultured plantlets of Quercus acutissima Carr. and Pinus densiflora Sieb. et Zucc. after addition of Pisolithus tinctorius(Pt) ectomycorrhizal fungus inoculum to the medium and effects of three levels of sucrose and phosphorus in culture media. Shoots of Quercus acutissima were obtained from winter buds of a 30-year old tree and cuttings of Pinus densiflora from germinated seed, and they were inoculated with Pt in vitro. In both species, Pt enhanced shoot length, survival, number of adventitious roots, root length, and rooting percentage. Survival in Quercus acutissima was increased from 75% in control to 100% in Pt inoculation. Pt inoculation increased the percentage of rooting from 20% to 70% in Quercus acutissima cuttings and from 63% to 100% in Pinus densiflora cuttings. It is concluded that mycorrhizal inoculation to tissue cultured Quercus acutissima Carr. and to in vitro cutting of Pinus densiflora Sieb. et Zucc. has practical application to improvement of poor root development and initial period of reduced shoot growth in vitro.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.50-61
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• 2018

A precedent defense project study was carried out in the early stage of the project to support efficient determination of the acquisition method of the determined weapons system. A precedent study in the early stage of the project has been neglected, but lack of performance and increased costs are continuing problems. In a precedent study on a number of items within a limited time frame, expected risks during project implementation were not identified and reduced by failing to review the key review element in depth. Therefore, in this paper, we studied the application of the FTA/FMEA technique used in the system safety analysis process to identify key factors to be considered when carrying out the defense research project. The methodology for the development of the key review items was established through the improvement of the FTA/FMEA technique in order to meet specific conditions and given conditions. Based on the results of this study, we redefined core key factors in the precedent study stage, thereby eliminating unnecessary and anticipated risks that may arise in the future project management aspects of the defense project. It is anticipated that this will reduce costs and time in terms of the lifecycle of the weapon system and promote stable operation of the project through reduction of risk.