• BMB Reports
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• v.39 no.3
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• pp.263-269
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• 2006

In the genome of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, open reading frame 39 (Ha39) is the only gene predicted to encode an RNA recognition protein. Computer analysis revealed that Ha39 homologues were found in 15 NPVs, but not in GVs. Its transcripts were detected from 3 through 72 hours post infection (h p.i.) using RT-PCR and Northern blot analysis. The protein was detected in infected-cell lysates from 6 h p.i. Western blot assay of ODV and BV preparations revealed that Ha39 encodes a structural protein associated with BVs. Additionally, immunofluorescence microscopy demonstrated that the protein was present within cytoplasm in virus-infected cells, but not in the nuclear region.

• The Plant Pathology Journal
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• v.22 no.4
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• pp.334-338
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• 2006

Chrysanthemum chlorotic mottle viroid(CChMVd) isolates have been identified from chrysanthemum showing yellow spots or infected without symptom. They were 399-400 nucleotides length of RNA. CChMVd-SSHA6(GenBank accession no. DQ450682) revealed a GAAA to DUUC substitution in positions 82-85 of CChMVd-MSIN34(GenBank accession no. DQ402041). In vitro RNA transcripts with the complete CChMVd sequence were infectious and induced the typical CChMVd infection symptom of yellow spots in chrysanthemum cv. Sharotte. CChMVd caused reduction in growth in some cultivars, whereas some cultivars were not affected. This is the first report on the occurrence of CChMVd in chrysanthemum in Korea.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.5-5
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• 2002

Nucleoside diphosphate kinases (NDPKs) are conserved through evolution and have been shown to be involved in various biological phenomena. By functional screening in yeast, we identified a new member of the NDPK family, ㎚23-M5, which encodes a 211-amino acid protein with 86% identify to the human homolog, ㎚23-H5. Northern blot analysis reveals that ㎚23-M5 encodes two transcripts of 0.8 and 0.7 kb, which are highly and specifically expressed in adult testis. Reverse transcriptase polymerase chain reaction analysis shows that nm23-M5 first appears in pachytene spermatocytes and increase chain reaction in abundance through subsequent stages. (omitted)

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.82-82
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• 2002

The Wnt signaling pathway plays pivotal roles in embryonic development and oncogenesis through various signaling molecules inculding Frizzled receptor, recently characterized LRP5/6 and Dickkopf protein. Although Wnt signaling has been characterized in both developmental and oncogenic processes, little is known about its function in the normal adult. The ability of LRP5 to bind apolipoprotein E(apoE) and the abundant expression of LRP5 transcripts in hepatocytes, raise the possibility that LRP5 plays a role in the hepatic clearance of ApoE-containing chylomicron remonants, a major plasma lipoprotein carrying diet-derived cholesterol. (omitted)

• Journal of Computing Science and Engineering
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• v.3 no.4
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• pp.218-237
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• 2009

Interactions are intrinsic part of what we do. We interact when we work, when we learn, when we visit a doctor, and when we play. With the advent of information and communications technology we can collect rich data (video, audio, and various transcripts including text chat) about such interactions. This opens an opportunity to monitor the dynamics of interactions and to get deeper insights of how they unfold and deliver this information to the interacting parties. This paper presents the design of a technology for visualising information about the dynamics of unfolding of interactions and presenting it in an ambient display on mobile devices. The purpose of this technology is the delivery of such information to the point of decision making.

• Journal of the Korean Society for information Management
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• v.37 no.3
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• pp.177-202
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• 2020

In this study the author examines the health information seeking behavior of older adults from the perspective of cognitive load. Using a qualitative method, based on ground theory, data were collected using in-depth interviews. Twenty-eight participants were participating older adults living in G community city, South Korea who experienced seeking health information. Interview transcripts were analyzed using a qualitative thematic coding framework. Three themes describing older adults' health information seeking behaviors were focused: intrinsic, extraneous, and germane cognitive load. The findings are geared towards the context of older adults' information seeking to support an understanding of successful information seeking behaviors in this population and to contribute to their health and well-being. The intent is to present information on current research in the field of Library and Information Science and to demonstrate how a cognitive load approach can be used to address the problems of older adults' health information seeking behaviors.

• Annual Conference on Human and Language Technology
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• 2018.10a
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• pp.204-209
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• 2018

긴장이라는 측면은 의사소통을 하거나 글을 읽을 때 사람에게 항상 영향을 주고 있다. 긴장의 개념은 자연언어처리 분야에서 광범위한 의미로 사용되어 왔는데, 본 논문은 이런 개념 중 강연과 같은 한 방향 대화에서 화자의 말에 대하여 청중이 가지는 긴장도에 집중하여 이를 정량화하는 방법을 제안한다. 한 명의 저자에 의해 서술된 문서에 긴장도 개념을 적용함에 있어, 한 방향 대화에서의 긴장도를 정량화하는 본 연구는 긴장도 개념을 일반 문서에 적용할 때에 보다 용이하게 활용될 것으로 예상한다. 본 연구에서는 먼저 화자의 말에 대한 청중의 긴장도가 주석되어 있는 새로운 말뭉치를 구축하였다. 또한 문맥을 고려하여 긴장도를 예측할 수 있는 모델과 이에 따른 긴장도 분류 성능에 대한 실험 결과를 통하여 자동 긴장도 분류가 계산적으로 가능하다는 것을 보인다.

• Journal of Korean Academy of Nursing
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• v.31 no.6
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• pp.967-977
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• 2001

This study explored and described the living spiritual experience and was attempted to gain an understanding of spirituality. This was done by eliciting participants′ verbal descriptions of their experiences. Method: Data was obtained from in-depth interviews with one cancer patient, one pastor, and three missionaries after obtaining informed consent from each. Interviews were tape recorded and transcribed verbatim. The transcripts were analyzed using Colaizzi′s phenomenological method. Sandelowski′s evaluation criteria for qualitative research, such as reliability, suitability and auditability, were also used to establish reliability and validity of this study. Result: The five major theme clusters that designated the essence of the spiritual experience, "spiritual awareness," "interconnectedness," "love," "tran- scendent energy," "purpose and meaning in life," emerged from the analysis. Conclusion: This study revealed that spirituality was activated by awareness through introspection and interconnectedness with a Supreme Being. The interconnectedness with a Supreme Being played an important role in harmonious relationships with others and self. It also resulted in revealing the other beneticial attributes of spirituality. Love, the core concept of the interconnectedness, worked as a transcendent energy. Also, the ability to see beyond reality and resulted in finding meaning in life and accomplishing well being.

• Molecules and Cells
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• v.24 no.1
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• pp.37-44
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• 2007

Three catalase cDNA clones were isolated from the small radish (Raphanus sativus L.). Their nucleotide and deduced amino acid sequences showed the greatest homology to those of Arabidopsis. Genomic Southern blot analysis, using RsCat1 cDNA as a probe, showed that catalases are encoded by small multigene family in the small radish. Nondenaturing polyacrylamide gels revealed the presence of several catalase isozymes, the levels of which varied among the organs examined. The isozyme activities were assigned the individual catalase genes by Northern analysis using total RNA from different organs. The three catalase genes were differentially expressed in response to treatments such as white light, xenobiotics, osmoticum, and UV. Their expression in seedlings was controlled by the circadian clock under a light/dark cycle and/or in constant light. Interestingly, RsCat1 transcripts peaked in the morning, while those of RsCat2 and RsCat3 peaked in the early evening. Our results suggest that the RsCat enzymes are involved in defense against the oxidative stress induced by environmental changes.

• Journal of Genetic Medicine
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• v.10 no.1
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• pp.27-32
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• 2013

Post-transcriptional nucleotide sequence modification of transcripts by RNA editing is an important molecular mechanism in the regulation of protein function and is associated with a variety of human disease phenotypes. Identification of RNA editing sites is the basic step for studying RNA editing. Databases and bioinformatics resources are used to annotate and evaluate as well as identify RNA editing sites. No method is free of limitations. Correctly establishing an analytic pipeline and strategic application of both experimental and bioinformatics methods constitute the first step in investigating RNA editing. This review summarizes modern bioinformatics approaches and related resources for RNA editing research.