DOI QR코드

DOI QR Code

Occurrence of Chrysanthemum chlorotic mottle viroid in Chrysanthemum(Dendranthema grandiflorum) in Korea

  • Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Dong-Chan (Yesan Chrysanthemum Research Station) ;
  • Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration) ;
  • Cho, Jeom-Deog (National Horticultural Research Institute, Rural Development Administration)
  • Published : 2006.12.01

Abstract

Chrysanthemum chlorotic mottle viroid(CChMVd) isolates have been identified from chrysanthemum showing yellow spots or infected without symptom. They were 399-400 nucleotides length of RNA. CChMVd-SSHA6(GenBank accession no. DQ450682) revealed a GAAA to DUUC substitution in positions 82-85 of CChMVd-MSIN34(GenBank accession no. DQ402041). In vitro RNA transcripts with the complete CChMVd sequence were infectious and induced the typical CChMVd infection symptom of yellow spots in chrysanthemum cv. Sharotte. CChMVd caused reduction in growth in some cultivars, whereas some cultivars were not affected. This is the first report on the occurrence of CChMVd in chrysanthemum in Korea.

Keywords

References

  1. Ambros, S. Hernandez, C. Desvignes, J. C. and Flores, R. 1998. Genomic Structure of three phenotypically different strains of Peach latent mosaic viroid: implications of the existence of constraints limiting the heterogeneity of viroid qasispecies. J. Virol. 72:7397-7406
  2. Bouwen, I. and van Zaayen, A. Z. 1995. Chrysanthemum. In: Virus and virus-like diseases of bulb and flower crops, ed. by G. Loebenstein, R.H. Lawson and A.A. Brunt. pp. 396-408. John Wiley & Sons, New York
  3. Chung, B. N., Lim, J. H., Choi, S. Y., Kim, J. S. and Lee, E. J. 2005. Occurrence of Chrysanthemum stunt viroid in chrysanthemum in Korea. Plant Pathol. J. 21:377-382 https://doi.org/10.5423/PPJ.2005.21.4.377
  4. Diener, T. O. 1993. Viroids: Smallest and simplest agents of infectious disease. How do they make plants sick? Intervirology 35:186-195 https://doi.org/10.1159/000150309
  5. Diener, T. O., Hadidi, A. and Owens, R. A. 1977. Methods for studying viroids. In: Methods in Virology. vol. 6, ed. by K. Maramorosch and H. Koprowski. pp. 185-217. Academic Press, New York
  6. Diener, T. O. and Lawson, R. H. 1973. Chrysanthemum stunt: A viroid disease. Virology 51:94-101 https://doi.org/10.1016/0042-6822(73)90369-3
  7. Dimock, A. W. and Geissinger, C. M. 1969. A newly recognized disease of chrysanthemums caused by a graft-transmissible agent. Phytopathology 59:1024 (abstr.)
  8. Dimock, A. W., Geissinger, C. M. and Horst, R. K. 1971. Chlorotic mottle: a newly recognized disease of chrysanthemum. Phytopathology 61:415-419 https://doi.org/10.1094/Phyto-61-415
  9. Dusi, A. N., Fonseca, M. E. N. and Avila, A. C. 1990. Occurrence of a viroid in chrysanthemum in Brazil. Plant Pathol. 36:363-637
  10. Hooftman, R., Arts, M. J., Shamloul, A. M., van Zaayen, A. and Hadidi, A. 1996. Detection of Chrysanthemum stunt viroid by reverse transcription-polymerase chain reaction and by tissue blot hybridization. Proceedings IX Intl. Sym. Virus Dis. Ornam. Plants. Acta Hort. 432:120-128
  11. Horst, R. K. 1987. Chrysanthemum chlorotic mottle, In: The viroids, ed by T.O. Diener, pp. 291-295. Plenum, New York
  12. Kusunoki, M., Matsumoto, Y., Nakanishi, M., Soichi, N. and Sogou, K. 1993. Occurrence of chrysanthemum stunt disease in Kagawa Prefecture. Kagawaken Agricultural Experiment Station Research Report 44:19-25
  13. Matthews, R. E. F. 1991. Viroids, satellite viruses, and satellite RNAs. In: Plant virology: 3rd ed., pp. 306-325. Academic Press, New York
  14. McGovern, R. J., Horst, R. K. and Dickey, R. S. 1988. Reduced susceptibility of florists' chrysanthemum to Erwinia chrysanthemi following viroid infection. Acta Hort. 234:99-107
  15. Monsion, M., Macquaire, G., Bachelier, J. C., Faydi, C. and Dunez, J. 1980. Detection of chrysanthemum stunt and chlorotic mottle viroids by slab gel electrophoresis. Acta Hort. 110:321-328
  16. Navarro, B. and Flores, R. 1997. Chrysanthemum chlorotic mottle viriod: Unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes. Proc. Natl. Accad. Sci. USA. 94:11262-11267
  17. Paludan, N. 1980. Chrysanthemum stunt and chlorotic mottle: Establishment of healthy chrysanthemum plants and storage at low temperature of chrysanthemum, carnation, campanula, and pelargonium in tubes. Acta Hort. 110:303-313
  18. Pena, M. D. L., Mavarro, B. and Flores, R. 1999. Mapping the molecular determinant of pathogenicity in a hammerhead viroid: A tetraloop within the in vivo branched RNA conformation. Proc. Natl. Acad. Sci. USA. 96:9960-9965
  19. Romaine, C. P. and Horst, R. K. 1975. Suggested evidence for viroid etiology of chrysanthemum chlorotic mottle disease. Virology 64:86-95 https://doi.org/10.1016/0042-6822(75)90081-1
  20. Shiwaku, K., Iwai, T. and Yamamoto, Y. 1996. Cloning and nucleotide sequence of Chrysanthemum stunt viroid. Hyogo Pre. Agric. Inst. (Agriculture). 44:1-4
  21. Singh, B. P., Gupta, R. P., Abidi, S. M. H. and Raizada, R. K. 1978. Chlorotic mottle of Chrysanthemum morifolium (Ram.). A new record from India, Curr. Sci. 47:913

Cited by

  1. Incidence of Coleus blumei viroid 1 in Seeds of Commercial Coleus in Korea vol.24, pp.3, 2008, https://doi.org/10.5423/PPJ.2008.24.3.305
  2. Loop-mediated isothermal amplification for the rapid detection of Chrysanthemum chlorotic mottle viroid (CChMVd) vol.193, pp.1, 2013, https://doi.org/10.1016/j.jviromet.2013.05.016