• Title/Summary/Keyword: thick smear

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A survey of helminthic infections in the residents of rural areas near Ulaanbaatar, Mongolia

  • Lee, Do-Sung;Chung, Byung-Ha;Lee, Nam-Seok;Nam, Ho-Woo;Kim, Joong-Ho
    • Parasites, Hosts and Diseases
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    • v.37 no.3
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    • pp.145-147
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    • 1999
  • A total of 738 samples was collected to survey the helminthic infections of residents in two rural areas near Ulaanbaatar, Mongolia for 2 weeks from July 23 to August 2, 1998. Among 391 scotch-taped slides of anal swabs of children and of young teenagers, Enterobius vermicularis eggs were detected in 138 cases (35.3%). With the fecal samples of 206 Kato-Katz thick smear slides from adults, the eggs of E.vermicularis were observed in 9 cases and Taenia sp.in one case, respectively. And by ELISA on 141 blood samples absorbed to blood sampling paper,12 cases (8.5%)were found to be positive against the hydatid cyst antigen. Enterobiasis and hydatidosis are two major endemic diseases which are related closely to the life style of Mongolian.

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A STUDY ON MORPHOLOGIC CHANGES OF DENTINAL SURFACES BY DENTIN CONDITIONING AGENTS (상아질표면처리제에 의한 상아질표면의 형태변화에 관한 연구)

  • Lee, Eung-Hoon;Choi, Ho-Young;Min, Byung-Soon;Park, Sang-Jin;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.18 no.1
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    • pp.173-186
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    • 1993
  • The purpose of this study is to observe the morphological changes of prepared dentin surfaces by 5 dentin conditioning agents. Freshly extracted 48 healthy human molars were used in this study. The teeth were stored at $4^{\circ}C$ physiologic saline solution befor experiment. The teeth were cross-sectioned to expose dentin below 3.0mm at the cusp tip and above 2.0mm at the cemento-enamel junction with Crystal Cutter (MC411 D, Maruto Co., Japan). The specimens were then divided into 12 groups. The sectioned dentin surfaces in group 1, 3, 5, 7, 9, and 11 were prepared with No. 301 diamond point under air-water spray and those in group 2, 4, 6, 8, 10, and 12 were prepared with No. 700 carbide bur. The prepared dentin surfaces were conditioned with Nitric acid, Citric acid, Poly acrylic acid, EDTA, and Phosphoric acid. All the specimens were gold-coated with Eiko ion coater (Eiko-engineering Co.) and observed in Hitachi S-2300 Scanning electron microscope at 20 KV. The following results from this study were obtained; 1. The dentinal smear layers prepared with diamond point were compacted than those prepared with the carbide bur. 2. The dentinal smear layers prepared with diamond point or carbide bur were thick but after treatment of dentin conditioning agents smear layers were removed almost. 3. Irrespective of the uses of the diamond point or the carbide bur the morphological changes of dentin surfaces treated with the same conditioning agents were similar. 4. Treatment of nitric acid and EDTA was a little effect in removing dentinal smear layer. 5. Treatment of citric acid and phosphoric acid removed the smear layer very effectively and showed dissolution of peritubular dentin and opening of dentinal tubules.

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Curschmann's Spirals in Cervico-Vaginal Smears (자궁 경질부 도말 표본에서 관찰된 Curschmann 나선체 검색)

  • Park, In-Ae;Ham, Eui-Keun
    • The Korean Journal of Cytopathology
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    • v.2 no.1
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    • pp.56-61
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    • 1991
  • Curschmann's spirals morphologically similar to those seen in sputum were found in cervico-vaginal smears of six patients ranged from 28 to 40 years of age, during 18 months from January. 1985 to June 1986. The prevalence was 1 in 2147 smears in that period. All of them had gynecologic disorders without systemic effect such as chronic cervictis in three, leiomyoma in one, pelvic inflammatory disorder in one, and primary infertility in one. The Curshmann's spirals in the smear showed varying degrees of maturation from wavy incipient ones to highly celled mature ones, admixed with thick mucinous back-ground, suggesting of their production in the uterine cervix itself. Also the recent history of undergone cryocautery, electrocautery or parturition suggest its production in the endocervical gland due to mechanical obstruction and/or change in biochemical composition of mucus.

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Comparative Study on the Effectiveness of Modified Kato's Cellophane Thick Smear and Stoll's Dilution Egg Counting Technique for Quantitative Fecal Examination of Helminth Eggs (윤충란 정량검사를 위한 Stoll 희석충란계산법과 Kato 후층도말변법의 비교)

  • 홍성종;우호춘
    • Parasites, Hosts and Diseases
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    • v.30 no.2
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    • pp.141-146
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    • 1992
  • A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E,C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were $47.86{\times}10^{0.87{\;}logM.C.T.S.},{\;}$ in A. lumbricoides, $41.69{\times}10^{0.82{\;}logM.C.T.S.}$ in T. trichiura and $63.10{\times}10^{0.85{\;}logM.C.T.S.}$ in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.

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Diagnostic Value of the Intradermal Test for the Infection with Clonorchis sinensis (간흡충증 진단을 위한 피내반응검사의 의의)

  • Kim, Jong-Ho;Yun, Bong-Yung;Lee, Heun-Ju;Lee, Hyun-Woo
    • Journal of Yeungnam Medical Science
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    • v.5 no.2
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    • pp.47-52
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    • 1988
  • The intradermal(ID) test has been widely used in Korea and several reports about the results of the ID test are known. We examined the egg of Clonorchis sinensis(C. s.) by ID test in 443, stool's egg-counting technique in 79 and direct smear(cellophane thick smear technique) in 1304 subjects. The results are as follows.: 1. The positive rate of C. s. was 3.8% out of 1304 persons. 2. The sensitivity of ID test was 82.1% out of 39 persons and, the specificity was 64.6% out of 404 persons. 3. The false positive of ID test 35.4% out of 404 persons and, the false negative was 17.9% out of 39 persons. Intradermal test is a rapid, sensitive and useful supplementary diagnostic tool for the detection of Clonorchiasis infection and must be used as screening test with direct smear of stool but cross reaction with other helminth infections and moderate false reaction are the main disadvantages in its practical application.

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The effect of a diode laser and traditional irrigants on the bond strength of self-adhesive cement

  • Tuncdemir, Ali Riza;Yildirim, Cihan;Ozcan, Erhan;Polat, Serdar
    • The Journal of Advanced Prosthodontics
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    • v.5 no.4
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    • pp.457-463
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    • 2013
  • PURPOSE. The purpose of this study was to compare the effect of a diode laser and traditional irrigants on the bond strength of self-adhesive cement. MATERIALS AND METHODS. Fifty-five incisors extracted due to periodontal problems were used. All teeth were instrumented using a set of rotary root canal instruments. The post spaces were enlarged for a No.14 (diameter, 1.4 mm) Snowlight (Abrasive technology, OH, USA) glass fiber reinforced composite post with matching drill. The teeth were randomly divided into 5 experimental groups of 11 teeth each. The post spaces were treated with the followings: Group 1: 5 mL 0.9% physiological saline; Group 2: 5 mL 5.25% sodium hypochlorite; Group 3: 5 mL 17% ethylene diamine tetra acetic acid (EDTA), Group 4: 37% orthophosphoric acid and Group 5: Photodynamic diode laser irradiation for 1 minute after application of light-active dye solution. Snowlight posts were luted with self-adhesive resin cement. Each root was sectioned perpendicular to its long axis to create 1 mm thick specimens. The push-out bond strength test method was used to measure bond strength. One tooth from each group was processed for scanning electron microscopic analysis. RESULTS. Bond strength values were as follow: Group 1 = 4.15 MPa; Group 2 = 3.00 MPa; Group 3 = 4.45 MPa; Group 4 = 6.96 MPa; and Group 5 = 8.93 MPa. These values were analysed using one-way ANOVA and Tukey honestly significant difference test (P<.05). Significantly higher bond strength values were obtained with the diode laser and orthophosphoric acid (P<.05). There were no differences found between the other groups (P> .05). CONCLUSION. Orthophosphoric acid and EDTA were more effective methods for removing the smear layer than the diode laser. However, the diode laser and orthophosphoric acid were more effective at the cement dentin interface than the EDTA, Therefore, modifying the smear layer may be more effective when a self-adhesive system is used.

A Study on the Intestinal Helminths of the Patients in a Leprosarium in Korea (전남 소록도 주민의 장내기생충 감염실태 및 조충의 치료성적)

  • 홍성태;홍성종이순형김익상
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.102-104
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    • 1983
  • A total of 2,026 leprosy patients of the National Sorokdo Hospital was examined their infestparasites by cellophane thick smear method in January, 1983. The egg positive cases of Taenia spp. were treated with bithionol and the segments of Taenia were collected for species identification. The results were as follows: 1. Total egg Positive rate of any kind helminth was 78.2% and cumulative total was 85.2%. The egg positive rate for each helminth was as follow; Taenia spp. 3.4%, Ascaris lumbricoides 4.5%, Trichuris trichiura 72.1%, Clonorchis sinensis 2.8% and other 0.05%. 2. A total of 66 Taenia eggpPositive cases was treated; out of them proglottids of Taenia were collected from 26 cases. All of the collected worms were identified as T. saginata. The results revealed signiBicantly high egg positive rate of T. trichiura. However, A. lumbricoides was found to be controlled considerably by repeated chemotherapy during past 3 years. If chromotherapeutic agent is replaced with oxantel-pyrantel tablet, better result is expected. No clue was found for prevalence of T. sodium from both human and the pig in the island.

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An Epidemiological study on clonorcohiasis at a Gum-River Basin (금강유역(부여읍, 현북리)에 있어서의 간흡훼증의 역학적 조사)

  • 조경진;윤오섭;추상규;신현성
    • Journal of Environmental Health Sciences
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    • v.8 no.1
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    • pp.61-66
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    • 1982
  • An epidemiological survey on 136 inhabitants of a Gum-River basin, Hyunbook-ri, Puyo-up, Puyo county, South ChoongCheong-Do, conducted from July 22, 1981 to July 25, 1981 with the purpose of studying Clonorchiasis, revealed the results as follows, 1. Positive skin reaction (wheal size larger than $60mm^2$) rate was 42% (55 positive reactors among the 131 studied), and the positive rates were all high at the age groups over twenty, while low at the below twenty. 2. In the stool examination, the employed cellophane thick smear method revealed the results as follows, overall infection rate of helminth: 65% (55 infected among 84 studied) - C. sinensis 33% (28/84), A. lumbricoides 5% (4/84), Taenia Sp 7% (6/84), T. trichiurus 32% (27/84). 3. The prevalence rate of C. sincrisis was highest in 40 - 49 year-old group by age: male 39% (11 infected/ 28 studied) female 14% (3/28), and not a single case was found in the lower age groups than twenty including school children.

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한국건강관리협회의 국제협력사업 '한ㆍ라오스 기생충퇴치사업'

  • Yun, Cheong-Ha
    • Journal of Korea Association of Health Promotion
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    • v.1 no.1
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    • pp.34-37
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    • 2003
  • The NGO program of "intestinal parasite Control among primary Schoolchildren of Lao PDR", supported by KOICA and implemented by KAHP, has started in 1999 and will be executed until 2004. Under cooperation of the LAo Ministry of Health, WHO, Korean Embassy and KOCIA, for the last 4 years, KHIP has conducted parasitological survey throughout the country, supply of medical equipment and anti-helminthics and treatment of positive people. The results of the survey is as below : A total of 29,846 stool specimens were collected from primary schoolchildren from May 2000 to June 2002 and examined once with the cellphone thick smear. By species, the rate for Ascaris lumbricoides was 34.9%, hookworm 19.1%, Trichuris trihiura 25.8%, Opisthorchis iverrini 10.9%, Taenia spp. 0.6% and Hymenolepis spp. 0.1%. The northern mountainous regions showed a higher prevalence of soil-transmitted helminths. The regions along the Mekong River such as Khammuane, Saravane or Svannakhet Province showed a higher prevalence of fish-borne parasites.

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Fine Needle Aspiration Cytology of Malignant Epithelial Mesothelioma of the Peritoneum (복강에 발생한 악성 상피성 중피종의 세침흡인생검)

  • Sung, Sun-Hee;Lee, Kwang-Gil
    • The Korean Journal of Cytopathology
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    • v.2 no.1
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    • pp.43-50
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    • 1991
  • A case of malignant epithelial mesothelioma of the peritoneum diagnosed by fine needle aspiration cytology is described. The smear showed many Individually scattered or clustered large round malignant epithelial cells intermingled with relatively small nonneoplastic mesothelial and mesenchymal cells. Papillary configurations with thick fibrous core were also seen. The malignant cells were virtually reminiscent of reactive mesothelial cells but they were larger in size and had more prominent nucleoli and more frequent binucleated or multinucleated cell formations than reactive mesothelial cells. The characteristic features of malignant cell of mesothelioma compared with the metastatic adenocarcinoma were relatively uniform cellular size, prominent round nucleoli, large round vesicular nuclei with finely granular chromatin pattern, smooth nuclear membrane, abundant glassy cytoplasm rather than bubbly mucin-containing cytoplasm and fuzzy cell border.

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