• Journal of the Korean Chemical Society
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• v.52 no.5
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• pp.530-535
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• 2008

• The Plant Pathology Journal
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• v.37 no.4
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• pp.396-403
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• 2021

Fire blight disease, caused by Erwinia amylovora, could damage rosaceous plants such as apples, pears, and raspberries. In this study, we designed to understand how E. amylovora affected other bacterial communities on apple rhizosphere; twig and fruit endosphere; and leaf, and fruit episphere. Limited studies on the understanding of the microbial community of apples and changes the community structure by occurrence of the fire blight disease were conducted. As result of these experiments, the infected trees had low species richness and operational taxonomic unit diversity when compared to healthy trees. Rhizospheric bacterial communities were stable regardless of infection. But the communities in endosphere and episphere were significanlty affected by E. amylovora infection. We also found that several metabolic pathways differ significantly between infected and healthy trees. In particular, we observed differences in sugar metabolites. The finding provides that sucrose metabolites are important for colonization of E. amylovora in host tissue. Our results provide fundamental information on the microbial community structures between E. amylovora infected and uninfected trees, which will contribute to developing novel control strategies for the fire blight disease.

• Journal of the Korean Association of Geographic Information Studies
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• v.14 no.3
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• pp.163-173
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• 2011

This paper, as a case study on the evaluation of nationwide-unit forest functions, targeting the entire Gyeongsangnam-do region, examined the integration measures for the evaluation of national, public and private forest functions, as well as GIS data problems and GIS data building methods. Also, the distribution and characteristics of Gyeongsangnam-do's forest functions were examined. First, as integration measures for forest function evaluation, evaluation unit was proposed as watershed unit, and GIS techniques were proposed to correct some patterns of errors shown in the watershed maps. Also, of GIS data used for forest function evaluation, maps of locations of saw mills to be revised, expressway interchange location maps, and population distribution maps were built nationwide. Based on watershed units, the forest functions of 20 cities and counties in Gyeongsangnam-do were evaluated, revealing that wood production function and forest recreation function potentials, high-ranking was high distributed throughout the site, while most functions potentials, low-ranking was low distributed. In forest function maps with the application of priority by city and gun, the area size was ranked in the order of forest recreation, timber production, natural conservation, water yield, living environment conservation, and prevention of natural disaster. Case analysis results for large areas can be used in evaluating nationwide forest functions.

• Journal of Korean Elementary Science Education
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• v.41 no.2
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• pp.432-442
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• 2022

This study aims to apply a maker education program in an online class for a unit in elementary science entitled "Life of a Plant." Furthermore, it aims to investigate the effect of the program on the interest of students in science and creative problem-solving. The theme of the proposed maker education program is "Making a fairy tale audio book with the theme: the life cycle of a plant." The program consists of activities intended to produce creative audiobooks using concepts learned from the TMI maker education model stage. The result points to the positive effect of maker education on the interest of elementary school students in science and creative problem-solving. Therefore, applying the maker education program to online classes for elementary science is effective in improving the interest of students. In addition, further research is required to effectively apply the maker education program to online classes in science.

• Molecules and Cells
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• v.20 no.3
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• pp.361-363
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• 2005

Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor-diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a = 56.1, b = 74.4, $c=80.0{\AA}$, ${\alpha}={\beta}={\gamma}=90^{\circ}$. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was $1.9{\AA}$ using synchrotron X-ray irradiation at beam line BL-18B at the Photon Factory, Japan.

• Journal of agriculture & life science
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• v.45 no.2
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• pp.1-7
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• 2011

Three evergreen ground-cover species were tested and evaluated for cold acclimation with different lights in central Korea to enrich the varieties of ground cover plants for over-wintering. Nandina domestica which recently started to be planted in central Korea had high chlorophyll unit with shade condition in both locations, Banpo and Gwacheon during winter. Light condition had no influence on chlorophyll unit in Banpo after April, meanwhile full sun condition decreased chlorophyll unit significantly in Gwacheon, which is exposed to cold wind from north. Ophiopogon japonicas had more than double chlorophyll unit in shade condition compared to that in full light condition in both investigation areas in February. In addition, higher value of L and b in shade condition had been maintained than in full sun condition throughout investigation period. However, leaves in Gwacheon were de-colorized in full sun condition after over-wintering. Pachysandra terminalis had more higher chlorophyll unit in shade condition compared to in full sun condition in both investigation areas in February. But light conditions had not effect the difference of chlorophyll unit of Gwacheon in April. Therefore, The present results suggest that evergreen ground covers with shade condition would be used as landscape materials in the capital area.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.171-177
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• 2012

A total of 94 tomato accessions were evaluated for the resistance to $Tomato$ $spotted$ $wilt$ $virus$ (TSWV) using a Sw5-2 SCAR marker and bioassay. PCR products of the marker were approximately 574 bp, 500 bp, and 462 bp, among which the longest was linked to TSWV resistance allele of Sw5-b. This allele was only found in three accessions (09-438, 10-318, and 10-321) in which some individuals showed apparent recovery or stem necrosis symptom to a tomato isolate of TSWV-pb1. Thirty-five individuals (one per each accession) which were non-infected by ELISA were selected for further observation. Among these, 26 individuals that did not show any symptom at 5 months after inoculation were confirmed for viral infection by RT-PCR. TSWV-specific PCR amplicon was weakly detected in all 26 individuals including 'Eureta', a commercial F1 possessing the resistance allele of Sw5-b. The resistant genes in the selected individuals may play an important role for reducing the viral concentration in tissues of inoculated tomato plants and seems to be quantitatively controlled by several factors including Sw5-b gene.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.707-710
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• 2003

A strong constitutive $P_{JH}$ promoter from Bacillus was applied to overexpress the end oxylanase gene in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the $P_{JH}$ promoter and endoxylanase promoter $(P_B)$, and introduced into Bacillus subtilis DB431 The total activities of the enzymes reached about 140 unit/ml by cultivation of B. subtilis DB431 harboring pJHKJ4 in LB glucose medium. Ultrafilteration is effective its yield is 70%.

• Animal Bioscience
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• v.35 no.1
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• pp.87-95
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• 2022

Objective: This study was conducted to investigate the effect of associating calcium butyrate with tannin extract, compared to an antimicrobial on the growth performance, incidence of diarrhea, intestinal histology, immune-expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor α (TNF-α) in piglets. Methods: Seventy-two piglets (36 barrows and 36 gilts) weaned at 28±2 d and initial body weight of 7.17±1.07 kg were allocated to 3 treatments in a randomized complete block design with 8 replicates per treatment and 3 animals per experimental unit. Treatments were composed of NC, negative control: basal diet without additives; PC, positive control: basal diet + 40 mg/kg of colistin sulfate; or BT, basal diet + calcium butyrate + tannin extract. The butyrate and tannin inclusion levels were 0.15% in the pre-starter phase and 0.075% in the starter phase. Incidence of diarrhea was monitored daily, and on d 14 and 35 of experiment, 1 animal from each experimental unit was slaughtered to collect intestinal samples. Results: No significant differences were observed for growth performance. The butyrate-and tannin-based additive resulted in reduced (p<0.05) incidence of diarrhea in piglets during d 1 to 14 and d 1 to 35 in comparison with the other treatments. Piglets that consumed the diet containing the calcium-butyrate and tannin showed a lower (p<0.05) crypt depth in the duodenum than those receiving the NC treatment at 14 d of experimentation. The BT treatment provided a lower (p<0.05) immune-expression of COX-2 at 14 d and TNF-α at 35 d in the duodenum. Conclusion: Association between calcium butyrate and tannin extract resulted in a significant decrease in the incidence of diarrhea and inflammatory process in the duodenum of piglets. Therefore, calcium-butyrate combined with tannin could be a part of an alternative program to reduce the use of antimicrobials in the diet of weaned piglets.

• Journal of The Korean Association For Science Education
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• v.17 no.1
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• pp.21-29
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• 1997

Many middle school students have difficulty in understanding biological concepts because too many concepts are presented in the textbook compared to limited school hours. So, it is necessary to select concepts which are indispensable. The purpose of this study is to select key concepts in the unit 'The Continuity of Life' by surveying students' and teachers' cognition on the concepts. In this study, 78 concepts were extracted from 'Science 3', unit II 'The Continuity of Life'. To survey how students and teachers think the concepts, Likert type questionnaires were made. 300 third grade middle school students and 34 biology teachers were selected by random sampling and the questionnaires were applied. The following results were obtained:1. Students thought 59 concepts out of 78 were important and the mean score of important level of the concepts was 3.60. Students thought 26 concepts out of 78 were difficult and the mean score of diffculty level'of the concepts was 3.26. The more they think the concepts important, the more they think them difficult (r=0.7462, p<0.001). 2. Teachers thought 55 concepts out of 78 were important and the mean score of important level of the concepts was 3.82. Teachers thought 33 concepts out of 78 were difficult and the mean score of diffculty level of the concepts was 3.31. The more they think the concepts important, the more they think them difficult (r=0.6138, p<0.001). 3. The selected concepts were considered more important by teachers than by students(t=2.0150, p<0.05). However, there was no significant difference in evaluating the difficulty level of the concepts(t=0.7327, p>0.05). 4. It was found that students have difficulty in understanding concepts when they are presented in the textbook to require formal preparation than concrete preperation(t=2.6612, p<0.05).