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Comparison of the Viability of Cryopreserved Fat Tissue in Accordance with the Thawing Temperature

  • Hwang, So-Min;Lee, Jong-Seo;Kim, Hyung-Do;Jung, Yong-Hui;Kim, Hong-Il
    • Archives of Plastic Surgery
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    • v.42 no.2
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    • pp.143-149
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    • 2015
  • Background Adipose tissue damage of cryopreserved fat after autologous fat transfer is inevitable in several processes of re-transplantation. This study aims to compare and analyze the survivability of adipocytes after thawing fat cryopreserved at $-20^{\circ}C$ by using thawing methods used in clinics. Methods The survival rates of adipocytes in the following thawing groups were measured: natural thawing at $25^{\circ}C$ for 15 minutes; natural thawing at $25^{\circ}C$ for 5 minutes, followed by rapid thawing at $37^{\circ}C$ in a water bath for 5 minutes; and rapid thawing at $37^{\circ}C$ for 10 minutes in a water bath. The survival rates of adipocytes were assessed by measuring the volume of the fat layer in the top layers separated after centrifugation, counting the number of live adipocytes after staining with trypan blue, and measuring the activity of mitochondria in the adipocytes. Results In the group with rapid thawing for 10 minutes in a water bath, it was observed that the cell count of live adipocytes and the activity of the adipocyte mitochondria were significantly higher than in the other two groups (P<0.05). The volume of the fat layer separated by centrifugation was also measured to be higher, which was, however, not statistically significant. Conclusions It was shown that the survival rate of adipocytes was higher when the frozen fat tissue was thawed rapidly at $37^{\circ}C$. It can thus be concluded that if fats thawed with this method are re-transplanted, the survival rate of cryopreserved fats in transplantation will be improved, and thus, the effect of autologous fat transfer will increase.

A study on newly developed herbal ointment for itching (새로 개발한 한방 피부소양증 치료제에 관한 연구)

  • Lee, Joon-Moo
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.21 no.3
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    • pp.132-139
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    • 2008
  • Objectives : There have been few studies on external treatment for itching. A clinical study was performed to evaluate the effect of a newly developed herbal ointment. Methods : A newly developed herbal ointment made from Rumex japonicus Houtt, Prunus persica(Linne) Batsch, Sesamum indicum L., and Sus scrota domestics Brisson., was given to 49 participants with itching to administer for approximately a month. Demographic characteristics, treatment experiences, symptom change, and other comments were self-reported on a pre-defined questionnaire. Results : Most frequent complaint was itching (24.01%), followed by sensitivity (19.60%), dryness(18.62%), frustration (13.72%) and insomnia (12.25%). Of 41 participants who had been given treatment for itching, around a half of them (n=21) have been treated with Korean Medicine, but all of them tried Western Medicine as well. The effect of the ointment was evaluated as excellent or good/fair (67.32%). Continuity was reported as excellent or good/fair (51.0%) and fragrance (73.44%) and color (75.48%) was reported as excellent or good/fair. Convenience, however, was assessed as good/fair (67.32%) and as inconvenient (22.4%). Conclusion : The newly developed herbal ointment seems promising for relieving itching and further research to standardize and develop this kind of ointment is warranted.

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Writing a Good Scientific Manuscript in Korean on the Quaternary Sciences (제4기과학 한글원고 바로 쓰기)

  • 장순근
    • The Korean Journal of Quaternary Research
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    • v.14 no.1
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    • pp.73-86
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    • 2000
  • Abstracts and papers written in Korean presented in the 26th meeting of the Korea Quaternary Association held at Kongju University in November, 1999 are reviewed with an attention to such as writing well in Korean. Paragraphs and sentences of manuscripts are dotted with words and expressions of foreign languages such as Japanese, Chinese, English, and very long sentences. Undesirable expressions include also contradictory meanings, difficult expressions using Chinese characters, and incorrect sentences and wordings, and etc. Some recommendations for good manuscripts written in Korean are suggested. They include reading of science books for general readers and drilling for writing good manuscripts in Korean. Writing manuscripts in ample time, discussions with colleagues, and open-minded mentality of authors to accept critics are included in the drilling for writing good manuscripts. Editorial board should also check the manuscripts before printing.

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Chondrogenesis of Periosteum-derived Progenitor Cells on Hyaluronic Acid Fiber Scaffold (Hyalograft $3D^{\circledR}$)

  • Shin, Hyun-Chong;Choi, Yong-Soo;Lim, Sang-Min;Lee, Chang-Woo;Kim, Dong-Il
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.239-243
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    • 2005
  • Periosteum-derived progenitor cells (PDPCs) were isolated and characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). The chondrogenesis of PDPCs was performed on hyaluronic acid fibers ($Hyalrograft^{\circledR}$) 3D) in chondrogenic induction medium. PDPCs showed the chondrogenic potential when cultured on hyaluronic acid fibers. These results showed that the characterized PDPCs were the chondrogenic progenitor cells and $Hyalrograft^{\circledR}$ 3D served as a useful carrier for PDPCs in transplantation proliferation, matrix synthesis and differentiation. Therefore, it could be used as a matrix for healing the defected cartilage.

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A study on consistency and accuracy of pulse diagnosis in Eight-Constitution Medicine

  • Shin, Yong-Sup;Nah, Seong-Su;Oh, Hwan-Sup;Park, Young-Jae;Park, Young-Bae
    • Advances in Traditional Medicine
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    • v.9 no.1
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    • pp.14-19
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    • 2009
  • The aim of this study is to evaluate appraiser's consistency and accuracy about pulse diagnosis (PD) in discrimination of eight-constitutions using Gage R&R study. Cumulative numbers of PD for discrimination of eight constitutions of three appraisers' experience were 75,000 cases, 50,000 cases, 1,100 cases, respectively. Three Appraisers diagnosed subject's eight-constitutions by PD with blinded method. Gage R&R study was used to verify the results. In the measurements of consistency, appraiser B (agreement = 80%, Value of k = 0.8276) was very good, appraiser A (agreement = 70%, Value of k = 0.7465) was good, and appraiser C (agreement = 50%, Value of k = 0.5365) was moderate. In the measurements of accuracy, appraiser B (agreement = 70%, Value of k = 0.6812) was good, appraiser A (agreement = 60%, Value of k = 0.6414) was good, and appraiser C (agreement = 0%, Value of k = -0.1000) was poor. The results suggest that accuracy of discrimination of constitutions relatively depend on experience and number of cases of PD. Further large controlled study is needed to evaluate the accuracy of PD.

Dyeing Properties and Functionality of Methanol Extract from Juniperus chinensis Heartwood (향나무 심재 메탄올추출물의 염색성과 기능성)

  • Nam, Ki Yeon;Lee, Jung Soon
    • Textile Coloration and Finishing
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    • v.25 no.3
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    • pp.194-205
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    • 2013
  • In this study, we report dyeing properties and functionality of cotton and silk fabrics dyed with methanol extract from Juniperus chinensis heartwood. Non-aqueous dye of Juniperus chinensis heartwood extracted with methanol was expressed reddish color. It showed relatively good affinity to silk than cotton. Dye uptake of cotton and silk continued to increase according to the increase of the dye concentration. Cotton was not affected by the dyeing temperature and time, however, dye uptake of silk was increased as the dyeing time and temperature were increased. Dye uptake of cotton and silk were the highest at pH 8 and pH 6 respectively. Dyed fabrics showed various colors depending on mordant types and mordant concentration. Washing, light and perspiration fastness were not good. However, rubbing and dry cleaning fastness showed relatively good grade. Dyed fabrics of ultraviolet-cut ability and deodorant ability were improved. Also, dyed fabrics showed very good antimicrobial abilities of 99.9%.

A Study on the User-contributed Reviews for the Next Generation Library Catalogs (차세대 도서관 목록의 이용자 서평에 관한 고찰)

  • Yoon, Cheong-Ok
    • Journal of the Korean Society for Library and Information Science
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    • v.46 no.2
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    • pp.115-132
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    • 2012
  • The purpose of this study is to examine the current status of user-contributed reviews for the Next Generation Library Catalogs, and the potential impact of user reviews available from the external sources, including Amazon.com and GoodReads.com. During the period of February 16th through April 4th, 2012, the number of holding libraries and user-contributed reviews, tags and reading lists of ten selected books were examined from the WorldCat. Also the user-contributed reviews for the same books available from Amazon.com and GoodReads.com were examined, and a case of reviews for one book was analyzed. The result shows that only a few users participated in the WorldCat, and user-contributed reviews were rarely used, when compared with tags or reading lists. Several hundred to thousand user-contributed reviews for the same books were available from Amazon.com and GoodReads.com directly linked with bibliographic records. A case of one book from Amazon.com reveals the possibility of distorting the function of user-contribution. With the introduction of the function of user-contribution, it is expected that its growth rate should be carefully observed and its potential impact on users should be thoroughly and systematically analyzed in the near future.

Surgically Treated Anterior Communicating Artery Aneurysm

  • Koo, Sang-Keun;Song, Yeung-Jin;Huh, Jae-Taeck
    • Journal of Korean Neurosurgical Society
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    • v.37 no.6
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    • pp.405-409
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    • 2005
  • Objective: The purpose of this study is to assess the factors related to the outcome of 84 patients who underwent surgery for anterior communicating Artery(ACoA) aneurysms. Methods: The authors review 84 patients who were undertaken from January 1998 to May 2004. In the management of ACoA aneurysms, the outcome was based on several factors: Clinical condition, Distribution of hemorrhage, Time between aneurysmal rupture and surgery, Direction and shape of the aneurysm. Results: The incidence rate of the ACoA aneurysm was 35%. Seventy four patients were classified as those having a good recovery, but 5 patients suffered from some morbidity and 5 patients died. The rate of good outcome for the patients with Hunt and Hess grade was as follows 100% in grade I, 95% in grade II, 80% in grade III, IV and V. The rate of good outcome for the patients with Fisher grade was as follows 98% in grade I, II and 81% in grade III, IV. Nineteen of 22 patients who underwent early surgery were rated as good, while twenty six of 30 patients for whom surgery was delayed showed a favorable result. The unfavorable outcomes were also attributed by vasospasm or other medical problems. Conclusion: For further improvement of the overall surgical outcome: First, early surgical intervention is recommended for good grade patients. Second, active management of poor grade patients should be scrutinized with early surgery. Third, it is also important to step up the effort to minimize the risk of medical complications to enhance surgical results on top of the mainstay of prevention efforts for vasospasm and rebleeding.

Changes in Free Amino Acid, Carotenoid, and Proline Content in Chinese Cabbage (Brassica rapa subsp. Pekinensis) in Response to Drought Stress

  • Shawon, Rayhan Ahmed;Kang, Baek Song;Kim, Ho Cheol;Lee, Sang Gyu;Kim, Sung Kyeom;Lee, Hee Ju;Bae, Jong Hyang;Ku, Yang Gyu
    • Korean Journal of Plant Resources
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    • v.31 no.6
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    • pp.622-633
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    • 2018
  • Chinese cabbage grown during autumn season is confronted with drought conditions for a certain period, especially during the early growth stage. In this study, we investigated the effects of drought stress on plant growth characteristics, as well as free amino acid, carotenoid, and proline in Chinese cabbage. Chinese cabbage seeds (Bulam Plus) were germinated, and all the seedlings were transplanted into plastic containers (28 cm diameter ${\times}$ 22 cm high) containing a commercial growth medium. The soil water content was measured and maintained at 10% for the drought-stressed plants and at 30% for the control plants, for three weeks. The results revealed that plant growth parameters were lower in the drought-stressed plants than in the control plants. The total free amino acid content tended to decrease in both drought-stressed and control plants with time. The total free amino acid content was found to be lower in the drought-stressed plants than in the control plants and the proline content was unaffected. Moreover, at three weeks after treatment, carotenoid content in drought stressed plants was significantly higher than that in the untreated plants. We believe that our study makes a significant contribution to the literature because the effects of drought stress on plant growth parameters, free amino acid, carotenoids, and proline accumulation in autumn growing cultivar of Chinese cabbage have not been widely studied in Korea, and our study provides valuable information in this regard, as Chinese cabbage is consumed throughout the year in Korea.

A Case Study on Application of Yeeki Feng Shui to The Buildings of City Center -Seoul Jongno-gu, A, B, C the Center of The Building (도심 빌딩의 이기적 풍수 적용사례에 관한 연구 -서울시 종로구 A, B, C빌딩을 중심으로-)

  • You, Chae-Myung;Cho, Sung-Je
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.7 no.2
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    • pp.92-99
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    • 2014
  • There have been many existing researches on buildings of Yangtaek that applies Hyeongki Feng Shui, but not enough case studies on buildings applying Hyungong Feng Shui. In this study, which is located in Jongno-gu, Seoul applying Hyeonggohg Feng Shui A, B, C of the building you want to study the case. The result is as follows: first, the A building turned out to have the longest earth phase among the selected buildings. This also means the A building has the longest good luck. Second, the four Shape 'Wangsanwanghyanng' were identified Building A and B buildings. This means those buildings have a good feng shui, facing water with mountains at the back. Third, the Good or bad of the buildings judged by the Yeeki feng shui turned out to be "good". This result shows that those buildings have been well-formed in time and space with a good feng shui, and they are considered to have a great development in corporate growth.