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Computation of residue limit of organophosphorus pesticides in functional foods from citrus fruit peels (감귤과피를 이용한 기능성 식품 중 유기인계 농약의 잔류기준 산정)

  • Lee, Mi-Kyung
    • Korean Journal of Environmental Agriculture
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    • v.18 no.4
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    • pp.349-354
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    • 1999
  • This study was conducted to propose residue limits of organophosphorus pesticides in functional foods produced from peels of citrus fruits. For these, not only reduction factors of pesticides in cooking and processing were estimated, but factors needed in order to produce residue limits were identified and their application method was devised. The results were as follows: (1) reduction factors appeared as 0.09-1.60 by different processings of various foods, (2) residue limits of pesticides on dietary fiber and bioflavonoid products could be computed from maximum residue limit of pesticides on the raw agricultural produce, reduction factors, and the anticipated amount of consumption of the functional foods. (3) residue limits of 18 organophosphorus pesticides were proposed in the range of 0.01-3.0mg/Kg on the dietary fiber product and 0.1-80mg/Kg on the bioflavonoid product from citrus fruit peels.

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Residual Characteristics of Insecticides Used for Oriental Tobacco Budworm Control of Paprika (파프리카 재배기간 중 담배나방 방제에 사용되는 살충제의 잔류특성)

  • Lee, Dong Yeol;Kim, Yeong Jin;Kim, Sang Gon;Kang, Kyu Young
    • Korean Journal of Environmental Agriculture
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    • v.32 no.1
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    • pp.84-93
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    • 2013
  • BACKGROUND: This study was carried out to investigate the residual characteristics of insecticides used for Oriental Tobacco Budworm control and to establish the recommended pre-harvest residue limit leading to contribution in safety of paprika production. METHODS AND RESULTS: The recommended Pre-Harvest Residue Limits (PHRLs) of insecticides during cultivation of paprika were calculated from residue analyses of insecticides in fruits 1, 3, 5, 7, 10, 12, 15, 18 and 21 days after treatment. Paprika samples were extracted with QuEChERS method and cleaned-up with amino propyl SPE cartridge and PSA, and insecticide residues were analyzed either by HPLC/DAD or GLC/ECD. The limits of detection were 0.01 mg/kg for 5 insecticides. Average recoveries were $81.3{\pm}1.62%$-$98.3{\pm}1.58%$ of 5 insecticides at fortification levels of 0.1 and 0.5 mg/kg. The biological half-lives of the insecticides were 8.5 days for bifenthrin, 11.8 days for chlorantraniliprole, 16.8 days for chlorfenapyr, 7.1 days for lamda-cyhalothrin and 31.3 days for methoxyfenozide at recommended dosage, respectively. CONCLUSION(S): The pre-harvest residue limits for 10 days before harvest were recommended 1.05 mg/kg, 1.41 mg/kg, 0.93 mg/kg, 2.06 mg/kg and 1.08 mg/kg as bifenthrin, chlorantraniliprole, chlorfenapyr, lamda-cyhalothrin and methoxyfenozide, respectively. This study can provide good practical measures to produce safe paprika fruit by prevention of products from exceeding of MRLs at pre-harvest stage.

Criteria for diet pattern and meal management to improve cognitive function: A systematic review (체계적 문헌 고찰을 통한 인지기능 개선에 도움을 주는 식사 관리에 대한 연구)

  • Park, Young-Sook;Lee, Hyun-Jung;Choi, Kui-Jeong;Xu, Lin;Nam, Ye-Rim;Kim, Yoon-Ha;Kim, Min-Ji;Shin, Weon-Sun
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.450-458
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    • 2020
  • The purpose of this study was to conduct a systematic review of the current published research related to improvement in cognitive function. A systematic search was performed in three bibliographic databases (PubMed, Cochrane Library, and EMBASE) using "dementia", "memory", "food", "diet", and "nutrition" as keywords. Meal management intervention, including Dietary Approaches to Stop Hypertension (DASH) diet, Mediterranean (Med) diet, Diet Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet, and other studies, was also included in the analysis. Through extensive screening, 21 articles, out of 2101 papers retrieved, were used for the final systematic review. The methodological quality of the randomized controlled clinical trials (RCTs) was assessed using the Cochrane Risk of Bias tool. These articles recommended vegetables, fruits, whole grains, olive oil, fish, berries, nuts, and beans. In conclusion, this study suggests the potential use of meal management to improve cognitive function.

Development and Evaluation of PCR-Based Detection for Pseudomonas syrinage pv. tomato in Tomato Seeds (토마토 종자로부터 PCR을 이용한 Pseudomonas syringae pv. tomato의 검출)

  • Cho, Jung-Hee;Yim, Kyu-Ock;Lee, Hyok-In;Yea, Mi-Chi;Cha, Jae-Soon
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.376-380
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    • 2011
  • The bacterial speck of tomato caused by Pseudomonas syringae pv. tomato leads to serious economic losses especially on fruits of susceptible genotype. Thus, Pseudomonas syringae pv. tomato is a plant quarantine bacterium in many countries including Korea. In this study, we developed specific PCR assays for detection of the bacterium from tomato seeds. A specific primer set is designed from the hrpZ gene for specific detection of Pseudomonas syringae pv. tomato. A 501 bp PCR product corresponding to hrpZ gene was amplified only form Pseudomonas syringae pv. tomato strains, but no PCR product was amplified from other tomato bacterial pathogens, such as Pseudomonas syringae pv. glycinea, P. syringae pv. maculicola, P. syringae pv. atropurpurea, P. syringae pv. morsprunorum, and from other P. syringae pathovar strains. The nested-PCR primer set corresponding to an internal fragment of the 501 bp sequence (hrpZ) gine was used to specific detection of Pseudomonas syringae pv. tomato in tomato seed. A 119 bp PCR product using nested PCR primer was highly specific and sensitive to detect low level of Pseudomonas syrigae pv. tomato in tomato seeds. We believe that the PCR assays developed in this study is very useful to detect Pseudomonas syringae pv. tomato from the tomato seeds.

Occurrence of Apple Scar Skin viroid and Relative Quantity Analysis Using Real-time RT-PCR (Apple Scar Skin viroid 발생상황 및 Real-time RT-PCR을 이용한 상대정량 분석)

  • Kim, Dae-Hyun;Kim, Hyun-Ran;Heo, Seong;Kim, Se-Hee;Kim, Min-A;Shin, Il-Sheob;Kim, Jeong-Hee;Cho, Kang-Hee;Hwang, Jeong-Hwan
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.247-253
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    • 2010
  • Apple scar skin viroid (ASSVd) is one of the smallest viral pathogens infecting fruits, especially apple, and causes a significant damage to fruit trees. ASSVd usually induced the skin-dapple ring symptoms, but in 'Fuji' varieties, corked spot were occurred on the fruit skin in 2009. This new symptom will be of great helpful to diagnosis ASSVd in sight. ASSVd was surveyed in apple and pear from 2009 to 2010 in Korea, and ASSVd was identified in 20 out of 1,193 trees. The infection rate was 1.7%. To screen the infectivity of ASSVd among apple cultivars, real-time RT-PCR was applied followed by designing of ASSVd specific primers based on highly conserved regions of several ASSVd isolates including Korean isolate. NADH dehydrogenase subunit 5 (nad 5) gene, which is mRNA of the mitochondrial gene, was used for internal control. In this study, ASSVd infected apples were classified into 12 groups depending on different symptoms and symptom severity (scaring, rusting or malformation). Taken together, this study suggested that real-time PCR analysis was more sensitive to detect the low copy of ASSVd on early viroid infected apple skins than regular RT-PCR method.

Growth and Flowering Characteristics of Polygonatum spp. (둥굴레 속(屬) 식물(植物)의 생육(生育) 및 개화(開花) 특성(特性))

  • Jang, Key-Hyun;Park, Jung-Min;Kang, Jin-Ho;Lee, Seong-Tae
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.2
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    • pp.142-148
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    • 1998
  • The Polygonatum spp. used in this study was collected from 1994 to 1997, and planted by species in Hamyang medicinal plant experimental station field. $30{\sim}40 $ species of Polygonatum spp. have been known to be distributed in the northern hemisphere, and $14{\sim}18$ species in Korea. In this study, collected seven species of Polygonatum spp. were investigated. Plant height was highest in Polygonatum stenophyllum followed by P. falcatum, P. humile, P. lasianthum var. coreanum.. Leaf length was longest in P. falcatum and shortest in P. odoratum and leaf width was the most wide in P. odoratum var. pluriflorum. P. stenophyllum had 111 flowers per stem. The other species had $14{\sim}22$ flowers per stem. The ratio of flower setting in each species was high at two but was 95.2% at above four in P. stenophyllum. The number of fruits was 14.8, 15.4 and 17.7 in P. odoratum, P. falcatum and P. humile, respectively. Other species did not produce fruit. Fruit setting percentage was high in P. odoratum as 81.5%. The number of branched rhizome doubled every year.

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Effect of Cultural Regions or Methods on Postharvest Physiological Characteristics and Qualities of Paprika Fruits (재배지역과 재배방법이 파프리카 과실의 수확 후 생리 및 품질에 미치는 영향)

  • Kang, Ho-Min;Choi, In-Lee;Kim, Il-Seop
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.325-329
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    • 2008
  • This study was conducted to compare the postharvest physiological characteristics of 2 paprika cultivars; 'Special', 'Fiesta' grown in 2 regions: Chuncheon(plain region) and Hwacheon(semi-high land region), and by 2 cultural methods; soil and hydroponic(rock wool culture). Respiratory rate of paprika fruit was higher in soil culture, and ethylene production was higher in Chuncheon region of both cultivars. As the thickness of fruit flesh showed thicker in Hwacheon region and hydroponic culture, the firmness of fruit was in proportion to thickness. Vitamin C content was higher in red-cultivar, 'Special'. and in Chuncheon region. Soluble solids was higher in 'Special', but there was no different between cultural regions. The fruit of both cultivars was grown smaller in Chuncheon region, and the fruit shape of 'Fiesta' progressed faster in soil culture. The surface color of fruit was higher in Hwacheon, and although there was no significantly different between cultural method, both cultivars grown by hydroponics was shown vivid color. As above results, for the long term circulation and high quality of fruit, paprika may be cultivated by hydroponics in high-altitude region.

The Effect of Carrot Juice, ${\beta}$-carotene Supplementation on Plasma Antioxidant Status of Korean Smokers (당근즙 및 ${\beta}$-Carotene의 섭취가 흡연자의 혈장 항산화 영양 상태에 미치는 영향)

  • Lee, Hye-Jin;Park, Yoo-Kyoung;Kang, Myung-Hee
    • Journal of Nutrition and Health
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    • v.42 no.8
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    • pp.750-758
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    • 2009
  • Smoking is associated with an increased incidence of numerous cancers and other degenerative diseases. It has been suggested that high consumption of fruits and vegetables may give some protection. Especially carrot is the most important source of dietary ${\beta}$-carotene. Therefore, the objective of this study is to investigate whether carrot juice supplementation to smokers have different or superior effect of compared to the effect supplementing purified ${\beta}$-carotene. The study was conducted in a randomized and placebo-controlled design, after a depletion period of 14 days, 48 smokers were supplemented either carrot juice (n = 18), purified ${\beta}$-carotene (n = 16) or placebo (n = 14). Each group was supplemented for 8 weeks with approximately 20.49 mg of ${\beta}$-carotene/day and 1.2 mg of vitamin C/day, as carrot juice (300 mL/day) or purified ${\beta}$-carotene (1 capsule/day). Plasma vitamin C, vitamin E and ${\beta}$-carotene level were significantly increased after carrot juice and ${\beta}$-carotene supplementation. These results suggest that carrot juice containing ${\beta}$-carotene or ${\beta}$-carotene itself have similar antioxidative potentials by increasing the antioxidant potential in smokers. Therefore, we suggest moderate dose of vitamin supplementation (amount of two servings of vegetable intake) may help to replenish the decreased oxidative stress levels in smokers.

Food Intake and Bio-physiological indicators in Korean Rural Adults (농촌지역 일부 성인이 섭취한 식품과 관련된 생리적 지수)

  • Goo, Je-Gi;Kim, Yun-Kyung
    • Journal of agricultural medicine and community health
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    • v.34 no.3
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    • pp.375-385
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    • 2009
  • Objectives: The objective of this study was to identify the association of food intake with bio-physiological indicators: blood pressure, body mass index (BMI), waist/hip ratio (WHR), cholesterol and urine sugar among rural people. Methods: The subjects were 890 inhabitants from 14 towns of G County in Honam province. Data were collected by interview and self-reported with structured questionnaires from April 6th to 30th 2005. Data were analyzed with the frequency, percentage, t-test, $x^2$-test, ANOVA, Scheffe test and ANCOVA using SPSS 12.0 program. Results: Pre-hypertensive and hypertensive group was 27.0%, overweight and obese 27.1%, more than 0.90 in WHR 42.0%, more than 201 mg/dl of cholesterol 23.9%, and positive urine sugar was 8.7% in general. Among eleven food groups, fruit intakes were more effective in normotensive group than in the others. Vegetables, liquors, and milk products were 0.90 WHR more effective than the others. Milk products and liquors in BMI, and grains, beans and seaweed in low cholesterol group were more effective than the others. Conclusions: Bio-physiological indicators are related significantly only with fruits, milk products, meats, cereal and liquors among eleven food categories. Further study on the relationship between food intake, physical activities, smoking, drinking and lifestyle with bio-physiological indicators are suggested.

Effects of Treatment of Bag Kinds, Bagging Time and Plant Oils on Fruit Cracking and Bitter Rot in Grapevines (포도에서 봉지의 종류, 처리시기 및 식물성오일 처리가 열과와 탄저병 발생에 미치는 영향)

  • Moon, Byung-Woo;Lee, Young-Cheul;Nam, Ki-Woong;Kim, Jung-Joo
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.143-149
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    • 2008
  • The effects of fruit bagging time, bag kinds, and plant oil treatment on fruit cracking, pathogenic decay, and quality in grapevines were studied. The occurrence of cracking fruit was not affect by bag kinds in 'Campbell Early'. But, bitter rot occurrence in 'Campbell Early' and occurrence of cracking fruit and bitter rot in 'Wase Campbell Early' decreased effectively compared the ones of conventional bag, window bag, calcium coated bag to non-bagging. The cracking fruit occurrence of and 'Kyoho' decreased significantly by bagging to each before July 5 of 'Campbell Early' and before June 29 and July 5 of 'Kyoho' grapevines. The cracking fruit and bitter rot occurrence by plant oil treatment decreased significantly in 'Wase Campbell Early', and increased calcium of fruit skin in 'Campbell Early'. The soluble solids in fruits were much reduced by window bag and the Hunter b value in fruit skin was reduced by calcium coated bag. Accordingly, treatment of Ca-coated bag and plant oil was become judgment to reduction effect of cracking fruit.