Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Residual Characteristics of Insecticides Used for Oriental Tobacco Budworm Control of Paprika

파프리카 재배기간 중 담배나방 방제에 사용되는 살충제의 잔류특성

  • Lee, Dong Yeol (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Yeong Jin (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Sang Gon (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kang, Kyu Young (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University)
  • 이동열 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 김영진 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 김상곤 (경상대학교 식물생명공학연구소) ;
  • 강규영 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원)
  • Received : 2013.02.12
  • Accepted : 2013.03.25
  • Published : 2013.03.31
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Abstract

BACKGROUND: This study was carried out to investigate the residual characteristics of insecticides used for Oriental Tobacco Budworm control and to establish the recommended pre-harvest residue limit leading to contribution in safety of paprika production. METHODS AND RESULTS: The recommended Pre-Harvest Residue Limits (PHRLs) of insecticides during cultivation of paprika were calculated from residue analyses of insecticides in fruits 1, 3, 5, 7, 10, 12, 15, 18 and 21 days after treatment. Paprika samples were extracted with QuEChERS method and cleaned-up with amino propyl SPE cartridge and PSA, and insecticide residues were analyzed either by HPLC/DAD or GLC/ECD. The limits of detection were 0.01 mg/kg for 5 insecticides. Average recoveries were $81.3{\pm}1.62%$-$98.3{\pm}1.58%$ of 5 insecticides at fortification levels of 0.1 and 0.5 mg/kg. The biological half-lives of the insecticides were 8.5 days for bifenthrin, 11.8 days for chlorantraniliprole, 16.8 days for chlorfenapyr, 7.1 days for lamda-cyhalothrin and 31.3 days for methoxyfenozide at recommended dosage, respectively. CONCLUSION(S): The pre-harvest residue limits for 10 days before harvest were recommended 1.05 mg/kg, 1.41 mg/kg, 0.93 mg/kg, 2.06 mg/kg and 1.08 mg/kg as bifenthrin, chlorantraniliprole, chlorfenapyr, lamda-cyhalothrin and methoxyfenozide, respectively. This study can provide good practical measures to produce safe paprika fruit by prevention of products from exceeding of MRLs at pre-harvest stage.

본 연구는 파프리카 재배 중 담배나방 방제에 사용되는 살충제의 잔류특성을 알아보고 추천 생산단계 잔류허용기준(Pre-Harvest Residue Limit, PHRL) 설정을 통하여 안전한 파프리카 생산에 기여하고자 수행되었다. 파프리카에 각각의 농약을 기준량 처리와 배량 처리로 살포한 후 1, 3, 5, 7, 10, 12, 15, 18 그리고 21일에 일정한 간격에 맞추어 파프리카 시료를 수확하여 잔류농약을 분석하고 생물학적 반감기를 산출한 다음 추천 생산단계 잔류허용기준(Pre-Harvest Residue Limit, PHRL)을 설정하였다. 시료들은 QuEChERS법으로 추출한 후 $NH_2$ SPE cartridge와 PSA을 이용하여 정제하고 HPLC/DAD와 GLC/ECD를 이용하여 기기분석을 하였다. 5개 살충제의 검출한계는 모두 0.01 mg/kg이며 회수율은 검출한계의 10배, 50배 농도인 0.1, 0.5 mg/kg가 되도록 3반복 처리하여 $81.3{\pm}1.62%$에서 $98.3{\pm}1.58%$였다. 시험 살충제의 생물학적 반감기는 bifenthrin에서 11.8일, chlorantraniliprole은 8.5일, chlorfenapyr는 16.8일, lamda-cyhalothrin은 7.1일, methoxyfenozide에서 31.3일로 나타났다. 생산단계 농약 잔류허용기준은 수확 10일 전 bifenthrin, chlorantraniliprole, chlorfenapyr, lamda-cyhalothrin 그리고 에서 methoxyfenozide에서 각각 1.05 mg/kg, 1.41 mg/kg, 0.93 mg/kg, 2.06 mg/kg 그리고 1.08 mg/kg으로 제안하였다.

Keywords

References

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