• Title/Summary/Keyword: thaw

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γ-LA-Supplementation to IVC for IVP Bovine Embryos

  • Gaja, A.;Meng, C.L.;Sato, M.;Nakajima, T.;Kubota, Chikara;Kojima, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.1
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    • pp.25-32
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    • 2010
  • The present study aimed to examine the effects of ${\gamma}$-linolenic acid (GLA) supplementation to in vitro culture (IVC) medium on in vitro developmental competence, freezability and morphology of in vitro matured and fertilized bovine embryos. In vitro produced (IVP) bovine zygotes were cultured in IVC medium supplemented with 0 (negative control), 15, 31, 62, 125, 250, 500 or 1,000 ppm GLA, 250 ppm linoleic acid albumin (LAA) and without any supplement as a control. Day 6 blastocysts derived from culture control were cultured in IVC medium containing either 62, 250 GLA or 250 LAA for 24 h, and at Day 7 were subjected to freezing or morphological examination by electron microscope. GLA 15 showed a tendency to have a higher cleavage rate at Day 2 (70.3%) than other groups. The hatching rate at Day 9 in LAA (38.2%) was significantly higher than the control and all treatment groups (p<0.05), while the blastocyst rate in LAA (32.4%) did not differ from those of 15 (30.5%), 31 (27.1%), and 62 GLA (33.1%) or the control (35.1%). GLA in concentrations of 125, 250, 500, and 1,000 ppm had significantly detrimental effect on the blastocyst rate compared to 15, 31 and 62 ppm GLA, LAA, and control groups (p<0.05). In contrast, the highest post-thaw survival rate (100%) was observed in the control group (p<0.01). Large lipid droplets were observed in the cytoplasm of trophoblastic cells, even in the control, but were abundant in GLA groups. Taking the results of the study into consideration, the addition of GLA to the culture medium for IVP bovine embryos at the dose of 15 ppm increased the developmental competence of zygotes and enhanced the cleavage rate up to Day 2. However, blastulation rate and post-thaw survival were not increased when GLA was added to the culture media.

Resistance to Freezing and Thawing of Concrete Subjected to Carbonation (탄산화를 받은 콘크리트의 동결융해 저항성)

  • Lee, Seung-Tae;Park, Kwang-Pil
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.623-631
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    • 2018
  • In this study, the degree of deterioration of concrete was investigated in the laboratory under conditions of carbonation and freeze-thaw cycling, which are the major causes of the deterioration of its performance. In this test, the carbonated concrete was subjected to combined freeze-thaw deterioration tests for up to 300 cycles, and its dynamic elastic modulus and compressive strength were measured. The evaluation of the effect of the water-binder ratio on normal concrete subjected to combined carbonization and freezing-thawing showed that its resistibility against such combined deterioration decreased more rapidly in the concrete with a water-binder ratio of 55 % compared with that having a water-binder ratio of 35 %. In the case where the concrete was blended with a mineral admixture consisting of fly ash and blast furnace slag at the same water-binder ratio, it showed an increase of its resistibility against combined deterioration.

Acid Corrosion Resistance and Durability of Alkali-Activated Fly Ash Cement-Concrete (알칼리활성 플라이 애쉬 시멘트-콘크리트의 산저항성 및 내구성)

  • Kang, Hwa-Young;Park, Sang-Sook;Han, Sang-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.1
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    • pp.61-68
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    • 2008
  • A new cementitious material has been developed, called alkali-activated fly ash cement(AAFC), which is used to produce AAFC-concrete for construction. The effect of acid attack, sodium chloride solution, carbonation, freeze-thaw cycling, and SEM, XRD analysis of the AAFC-concrete prepared using alkali-activated fly ash cement and OPC-concrete were experimentally investigated. It was found that the acid resistance of AAFC-concrete(35 MPa) prepared from alkali-activated fly ash at 85$^{\circ}C$ for 24 hrs is far better than OPC-concrete(35 MPa). Also, the AAFC-concrete(35 MPa) had a similar resistance of OPC-concrete(35 MPa) to attack, such as sodium chloride solution, carbonation and freeze-thaw cycling.

Physical Properties of Acetylated Sweet Potato Starches as Affected by Degree of Substitution (치환도가 초산 고구마전분의 물리적 특성에 미치는 영향)

  • Yoo, Byoung-Seung;Lee, Hye-Lin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.1048-1052
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    • 2011
  • This study examined the flow properties, paste clarity, freeze-thaw stability and gel strength of acetylated sweet potato starch (ASPS) pastes and gels as a function of degree of substitution (DS). ASPS showed high shear-thinning flow behaviors with high Casson yield stress ($\sigma_{oc}$). Consistency index (K), apparent viscosity ($\eta_{a,100}$) and $\sigma_{oc}$ values of ASPS increased with an increase in DS. In the temperature range of $25{\sim}70^{\circ}C$, ASPS followed an Arrhenius temperature relationship. The activation energies (Ea=13.2~14.3 kJ/mol) of the ASPS samples were much lower than that (18.1 kJ/mol) of the native sweet potato starch (SPS). ASPS gels showed better freeze-thaw stability with a significant decrease in syneresis (%) compared to SPS gel. The gel strength values of ASPS were much lower than that of SPS, and significantly decreased with an increase in DS. The clarity of native SPS paste increased after acetylation.

Changes in Insulation Performance of Organic Insulating Materials for Building Construction by Accelerated Durability Test Conditions (가속내구성 조건에 따른 건축용 유기계 단열재의 단열성능 변화)

  • Lim, Soon-Hyun;Lee, Gun-Cheol
    • Journal of the Korea Institute of Building Construction
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    • v.16 no.6
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    • pp.595-601
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    • 2016
  • The insulation performance of the insulation currently used in building structures is reflected only during design based on initial performance and the reduction in heat insulation performance due to the degradation of long-term durability is not reflected. This study reviewed the degradation of heat insulation performance due to the durability degradation of insulating materials through the accelerated durability test. The study findings showed that the foamed polystyrene insulation bead method did not show performance degradation due to aging in the standard environmental condition and laboratory accelerated test condition but the performance is degraded in the freeze-thaw test condition. On the other hand, in the case of the extrusion method, the degradation of the heat insulation performance was less in the freeze-thaw test condition, but the rapid performance degradation was caused by the release of the internal gas at the beginning of aging. In addition, the hard polyurethane foam insulation showed better initial insulation performance than other insulation materials, but the performance was found to be degraded somewhat under laboratory accelerated test conditions and freeze-thaw test conditions.

Effects of Mugwort on Physicochemical Properties, Paste, and Gel of Rice Flour (쑥이 쌀가루의 이화학적 성질, 페이스트, 겔에 미치는 영향)

  • Chung, Koo-Min
    • Korean Journal of Food Science and Technology
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    • v.25 no.6
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    • pp.626-631
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    • 1993
  • The effects of mugwort on some physicochemical properties of rice flour, on the viscosity of rice flour pastes (2 and 4%), on the hardness and the freeze-thaw stability of rice flour gels (20 and 40%), and on the degree of gelatinization (DG) of pastes and gels were investigated. By addition of mugwort, water holding capacity, swelling power, and solubility of rice flour were increased. The rice flour pastes became thicker by addition of mugwort, but viscosities of both the rice flour pastes and the rice flour-mugwort pastes, without noticeable difference between them, were decreased during storage at $25^{\circ}C\;and\;4^{\circ}C$ for 7 days. The rice flour gels became firmer by addition of mugwort and hardnesses of both the rice flour gels and the rice flour-mugwort gels were increased during storage, especially at $4^{\circ}C$. DGs, measured by glucoamylase digestion, of pastes and gels were decreased slightly during storage. Freeze-thaw stability of gels was not affected by addition of mugwort.

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On the prediction of unconfined compressive strength of silty soil stabilized with bottom ash, jute and steel fibers via artificial intelligence

  • Gullu, Hamza;Fedakar, Halil ibrahim
    • Geomechanics and Engineering
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    • v.12 no.3
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    • pp.441-464
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    • 2017
  • The determination of the mixture parameters of stabilization has become a great concern in geotechnical applications. This paper presents an effort about the application of artificial intelligence (AI) techniques including radial basis neural network (RBNN), multi-layer perceptrons (MLP), generalized regression neural network (GRNN) and adaptive neuro-fuzzy inference system (ANFIS) in order to predict the unconfined compressive strength (UCS) of silty soil stabilized with bottom ash (BA), jute fiber (JF) and steel fiber (SF) under different freeze-thaw cycles (FTC). The dosages of the stabilizers and number of freeze-thaw cycles were employed as input (predictor) variables and the UCS values as output variable. For understanding the dominant parameter of the predictor variables on the UCS of stabilized soil, a sensitivity analysis has also been performed. The performance measures of root mean square error (RMSE), mean absolute error (MAE) and determination coefficient ($R^2$) were used for the evaluations of the prediction accuracy and applicability of the employed models. The results indicate that the predictions due to all AI techniques employed are significantly correlated with the measured UCS ($p{\leq}0.05$). They also perform better predictions than nonlinear regression (NLR) in terms of the performance measures. It is found from the model performances that RBNN approach within AI techniques yields the highest satisfactory results (RMSE = 55.4 kPa, MAE = 45.1 kPa, and $R^2=0.988$). The sensitivity analysis demonstrates that the JF inclusion within the input predictors is the most effective parameter on the UCS responses, followed by FTC.

Performance Evaluation of Bridge Deck Materials based on Ordinary Portland Cement Concrete (보통 포틀랜드 콘크리트 기반 교면포장 재료 성능 평가)

  • Nam, Jeong-Hee;Jeon, Seong Il;Kwon, Soo Ahn
    • International Journal of Highway Engineering
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    • v.19 no.6
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    • pp.129-137
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    • 2017
  • PURPOSES : The purpose of this study is to develop bridge deck concrete materials based on ordinary Portland cement concrete, and to evaluate the applicability of the developed materials through material properties tests. METHODS : For field implementation, raw material (cement, fine aggregate, and coarse aggregate) properties, fresh concrete properties (slump and air content), strength (compressive, flexural and bond strength) gain, and durability (freeze-thaw resistance, scaling resistance, and rapid chloride penetrating resistance) performance were evaluated in the laboratory. RESULTS : For the selected binder content of $410kg/m^3$, W/B = 0.42, and S/a = 0.48, the following material performance results were obtained. Considering the capacity of the deck finisher, a minimum slump of 150 mm was required. At least 6 % of air content was obtained to resist freeze-thaw damage. In terms of strength, 51.28 MPa of compressive strength, 7.41 MPa of flexural strength, and 2.56 MPa of bond strength at 28 days after construction were obtained. A total of 94.9 % of the relative dynamic modulus of elasticity after 300 cycles of freeze-thaw resistance testing and $0.0056kg/m^2$ of weight loss in a scaling resistance test were measured. However, in a chloride ion penetration resistance test, the result of 3,356 Coulomb, which exceeds the threshold value of the standard specification (1000 Coulomb at 56 days) was observed. CONCLUSIONS : Instead of using high-performance modified bridge deck materials such as latex or silica fume, we developed an optimum mix design based on ordinary Portland cement concrete. A test construction was carried out at ramp bridge B (bridge length = 111 m) in Gim Jai City. Immediately after the concrete was poured, the curing compound was applied, and then wet mat curing was applied for 28 days. Considering the fact that cracks did not occur during the monitoring period, the applicability of the developed material is considered to be high.

Durability Characteristics of Limestone Powder added Concrete for Environment-Friendly Concrete (석회석미분말을 첨가한 친환경 시멘트콘크리트의 내구 특성)

  • Choi, Woo Hyeon;Park, Cheol Woo;Jung, Won Kyung;Jeon, Beom Joon;Kim, Gyu Seon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.5
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    • pp.59-67
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    • 2012
  • During the manufacturing of Portland cement, CO2 gas is also necessarily produced through both decarbonation of calcium carbonate and kiln burning. By partially replacing the Portland cement with limestone powder, which is an inert filler in a concrete mixture, CO2 consumption can be reduced in a construction field. This study is to investigate the fundamental durability characteristics of limestone powder added concrete. Experimental variable was the replacement ratio of limestone powder from 0% to 25% with 5% increment. Durability characteristics were investigated by resistance to freeze-thaw, alkali-silica reaction and de-icing chemical in addition to the properties of fresh concrete. From test results, it was observed that the addition of limestone powder did not significantly affect the resistance to freeze-thaw reaction and de-icing chemical. The addition of limestone powder reduced the occurrence potential of alkali-silica reaction by reducing an alkali content in Portland cement.

Effect of Cooking Method and Additives on the Freeze-Thaw Stability of Mung Bean Starch Paste for Preparation of Omija-eui

  • Han, Hyun-Jeong;Jang, Keum-Il;Bae, In-Young;Lee, Kwang-Yeon;Koo, Seung-Hyun;Kim, Mi-Kyung;Jun, Soo-Jin;Lee, Hyeon-Gyu
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1230-1236
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    • 2009
  • Optimum conditions for the freeze-thaw stability (FTS) of mung bean starch (MBS) paste as a main ingredient in omija-eui were investigated. For the optimization of the paste preparation condition, the FTS of MBS prepared by boiling in a shaking water bath (BMSW) or by pressure-cooking in an autoclave (PCMA) were analyzed using a response surface methodology (RSM). In addition, the effects of various additives such as gums, sugars, and emulsifier were evaluated on the FTS of MBS paste prepared under optimal conditions. The predicted maximal FTS of MBS paste prepared by the PCMA method (73%) was higher than that of the paste prepared by the BMSW method (36%). In case of additives, gellan gum and sodium alginate effectively prevented the syneresis of MBS paste in the BMSW method and in the PCMA method, respectively. The use of a fructose fatty acid ester as an emulsifier decreased syneresis in a dose-dependent, while the addition of sugars accelerated syneresis. Consequently, MBS paste for omija-eui preparation may be efficiently prepared by adding sodium alginate and fructose fatty acid ester under the optimal conditions of 4.3% MBS content, $121^{\circ}C$ heating temperature, and $89^{\circ}C$ cooling temperature by pressure-cooking in an autoclave.