• Environmental Mutagens and Carcinogens
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• v.10 no.2
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• pp.93-106
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• 1990

The Drosophila temperature-sensitive mutant shibire (shi) is paralyzed at restrictive temperature by a reversible block in synaptic transmission. To explore the functional relationship among shi gene products, viability and temperature-sensitive paralytic behavior were quantitaively analyzed for four shi alleles, shi$^{ts1}$, shi$^{ts2}$, shi$^{ts4}$, and shi$^{ST139}$, and their heteroallelic combinations. The hemizygous combination of shi alleles over deficiency was not completely lethal. shi$^{ts2}$ exhibited distinctively higher viability than other alleles. A novel behavior, bang sensitivity, was also found in shi/Df(1). This bang-sensitive paralytic behavior was compared with that of the typical bang-sensitive mutant flies. Heterozygotes, shi/+, are more severe in temperature sensitivity than deficiency hemizygotes, Df(1)/+. Heteroallelic combinations of shi were less sensitive to high temperature than homozygotes. Among all allelic combinations, shi$^{ts2}$/shi$^{ts4}$ showed an unexpected extreme reduction in temperature sensitivity. The results of allelic interactions among 4 shi alleles suggest that the shi mutations examined behave as antimorphic alleles and that the gene product of shi are likely to function in multimeric forms.

• Polymer(Korea)
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• v.28 no.1
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• pp.59-66
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• 2004

Thermosensitive poly(N-isopropylacrylamide) gels containing temperature-sensitive liposomes showing temperature-dependent sol-gel transition were prepared. The surface of temperature-sensitive liposome was modified with copolymers of N-isopropylacrylamide and octadecylacrylate, which exhibited a lower critical solution temperature at around 30 $^{\circ}C$ After mixing the modified temperature-sensitive liposomes with poly(N-isopropylacrylamide) solution, the temperature-sensitive 1iposomes formed physically cross-linked gels through heating the solution above their lower critical solution temperatures. The release of drug from temperature-sensitive liposomes was determined by measuring fluorescence intensity. The drug release from temperature-sensitive liposomes in poly(N-isopropylacrylamide) gel gradually showed sustained-release with increasing temperature.

• Journal of Magnetics
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• v.12 no.4
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• pp.161-164
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• 2007

Constant temperature regulation of a hybrid heater which is composed of a temperature sensitive ferrite with low permeability and a Cu tube is investigated for hyperthermia. The temperature sensitive ferrite is inserted into a Cu tube and its length and diameter are 10 mm and 3 mm. Below B=0.05 T, the measured temperature and the calculated one increased with the ratio of $B^{1/2}$ and agreed well with each other. Above B=0.05 T, the measured temperatures maintained constantly almost $50^{\circ}C{\pm}1.5^{\circ}C$ because of the influence of Curie temperature of the temperature sensitive ferrite. This result shows that the hybrid heater is able to regulate the temperature constantly at the rate of $50^{\circ}C{\pm}1.5^{\circ}C$.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.30 no.2 s.245
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• pp.153-160
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• 2006

A technique for measuring surface temperature field in micro scale is newly proposed, which uses temperature-sensitive fluorescent (TSF) dye coated on the surface and is easily implemented with a fluorescence microscope and a CCD camera. The TSF dye is chosen among mixtures of various chemical compositions including rhodamine B as the fluorescent dye to be most sensitive to temperature change. In order to examine the effectiveness of this temperature measurement technique, numerical analysis and experiment on transient conduction heat transfer for two different substrate materials, i. e., silicon and glass, are performed. In the experiment, to accurately measure the temperature with high resolution temperature calibration curves were obtained with very fine spatial units. The experimental results agree qualitatively well with the numerical data in the silicon and glass substrate cases so that the present temperature measurement method proves to be quite reliable. In addition, it is noteworthy that the glass substrate is more appropriate to be used as thermally-insulating locally-heating heater in micro thermal devices. This fact is identified in the temperature measuring experiment on the locally-heating heaters made on the wafer of silicon and glass substrates. Accordingly, this technique is capable of accurate and non-intrusive high-resolution measurement of temperature field in microscale.

• Mycobiology
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• v.38 no.1
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• pp.70-73
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• 2010

Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over $30^{\circ}C$. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from $30^{\circ}C$ to $37^{\circ}C$. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.

• Journal of the Korean Chemical Society
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• v.47 no.3
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• pp.257-264
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• 2003

This study has investigated the temperature-sensitive liposomes, which release anticancer drug(doxorubicin) at the hyperthermia temperature$(~40^{\circ}C)$. The temperature-sensitive liposomes were modified with a copolymers of N-isopropylacrylamide(NIPAAm) and acrylamide(AAm), which exhibit a lower critical solution temperature (LCST) at the hyperthermia temperature. The release of doxorubicin from the modified liposomes was determined by measuring the fluorescence intensity with changing temperature and time. The release of doxorubicin from liposomes modified with poly(NIPAAm-co-AAm) copolymer was increased significantly, because poly(NIPAAm-co-AAm) could undergo the conformational transition in the narrow hyperthermia temperature region$(~40{\pm}2^{\circ}C)$. Moreover, we observed that doxorubicin released from liposomes within 5 minutes, and the size of modified liposomes was 120~170 nm. In this study, we have prepared temperature-sensitive liposomes which could be controlled by temperature. They can be applied in the field of a drug delivery system for tumor targeting by temperature control.

• The Korean Journal of Food And Nutrition
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• v.1 no.1
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• pp.25-32
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• 1988

Properties of purified isocitrate lyase from Saccharomycopsis lipolytica ATCC 44601 and MX9-11RX8 temperature-sensitive mutant were investigated. Purified isocitrate lyase from temperature-sensitive mutant was indistinguishable from the wild type enzyme with respect to the isoelectric pH(5.3), the thermostability and Km value for threo-Ds-Isocitrate(about 0.2 mM). When isocitrate lyase induced by acetate minimal medium at 33$^{\circ}C$, MX9-11RX8 mutant did not express enzyme activity but did synthesize polypeptide chain whose electrophoretic mobilities were equal to those of the purified enzymes.

• Journal of the Korean Society of Clothing and Textiles
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• v.24 no.2
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• pp.199-204
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• 2000

This study was conducted to investigate the thermoregulatory responses and wearing behavior of the cold-sensitive men when exposed to the mild cold of 14$^{\circ}C$. Two sessions of experiment were carried out and five healthy young men for the cold-sensitive group(CSG) and four healthy young for the cold-insensitive group(CIG) participated in the study as subjects CSG maintained rectal temperature lower than CIG due to their thicker clothing resulted in larger decrease of rectal temperature. CSG maintained skin temperatures higher than CIG. CSG felt cooler than CIG but wore thicker clothing for thermal comfort and this made keep their sensation warmer. These results were discussed in terms of autonomic and behavioral temperature regulation.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1225-1230
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• 2004

The effects of substrate material on the local heating performance of microheaters are studied by both numerical analysis and experiment. Transient conduction analysis shows that the substrate material with low thermal conductivity is critical to the local heating and fast response. A measurement technique for surface temperature field in microscale is newly proposed, which uses temperature sensitive fluorescent dye coated on the surface. The measured surface temperature fields on microheater arrays fabricated on different substrates are presented.

• Macromolecular Research
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• v.8 no.2
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• pp.89-94
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• 2000

A semi-interpenetrating network (IPN) was prepared for a temperature-sensitive polymer system composed of sodium alginate and poly (N, N-dimethylaminoethyl methacrylate (DMAEMA)-co-ethyl acrylamide (EAAm)). The role of sodium alginate is to provide crosslinked network and that of poly(DMAEMA-co-EAAm) is to provide temperature responsiveness to the polymer system. Semi-IPN gel shows temperature-induced swelling transition at the same temperature of the lower critical solution temperature of poly(DMAEMA-co-EAAm) and its swelling kinetics is manipulated by the control of crosslinking densitv.